Helicoverpa zea nudivirus 2 (HzNV-2)
Average proteome isoelectric point is 6.56
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 113 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|G9I040|G9I040_HZNV2 Uncharacterized protein orf14 OS=Helicoverpa zea nudivirus 2 OX=1128424 GN=orf14 PE=4 SV=1
MM1 pKa = 7.69 SIQGGGCGADD11 pKa = 2.83 SSEE14 pKa = 5.12 CNDD17 pKa = 3.69 CQFDD21 pKa = 3.77 KK22 pKa = 11.33 AGTGEE27 pKa = 3.98 NVRR30 pKa = 11.84 RR31 pKa = 11.84 GQSCSTDD38 pKa = 3.21 AEE40 pKa = 4.29 PVLGSFEE47 pKa = 4.18 YY48 pKa = 10.47 TDD50 pKa = 4.85 CNPADD55 pKa = 4.1 TIAAGNIVDD64 pKa = 4.97 LKK66 pKa = 9.45 NVRR69 pKa = 11.84 YY70 pKa = 9.92 GSINTSVSSDD80 pKa = 3.43 CSEE83 pKa = 4.62 DD84 pKa = 3.36 SSNVYY89 pKa = 10.21 SNRR92 pKa = 11.84 ATISATPVVIQDD104 pKa = 3.91 SEE106 pKa = 4.83 DD107 pKa = 3.52 EE108 pKa = 4.14 QFMDD112 pKa = 5.1 DD113 pKa = 3.74 SKK115 pKa = 11.53 SVHH118 pKa = 6.21 KK119 pKa = 10.78 GSFIEE124 pKa = 4.11 YY125 pKa = 9.7 RR126 pKa = 11.84 AISKK130 pKa = 10.54 NEE132 pKa = 3.74 YY133 pKa = 8.89 NQNEE137 pKa = 4.37 YY138 pKa = 9.92 NQNEE142 pKa = 4.39 YY143 pKa = 9.93 NQNEE147 pKa = 4.4 YY148 pKa = 11.11 NKK150 pKa = 10.46 NEE152 pKa = 4.05 YY153 pKa = 8.77 NQNEE157 pKa = 4.36 YY158 pKa = 11.07 NKK160 pKa = 10.51 NEE162 pKa = 4.05 YY163 pKa = 8.68 TSKK166 pKa = 10.97 NDD168 pKa = 3.21 YY169 pKa = 9.91 NSTMRR174 pKa = 11.84 RR175 pKa = 11.84 CKK177 pKa = 9.41 LHH179 pKa = 5.6 RR180 pKa = 11.84 VKK182 pKa = 10.8 RR183 pKa = 11.84 SIVEE187 pKa = 4.22 SFEE190 pKa = 4.41 STDD193 pKa = 3.03 ATPNVDD199 pKa = 2.61 VDD201 pKa = 3.26 AMEE204 pKa = 4.98 RR205 pKa = 11.84 GRR207 pKa = 11.84 GASAIEE213 pKa = 4.29 KK214 pKa = 10.32 ANTMDD219 pKa = 3.73 NSNKK223 pKa = 10.43 DD224 pKa = 3.48 GVVEE228 pKa = 3.83 EE229 pKa = 4.98 SIVVVNGSTIDD240 pKa = 3.62 GSIVDD245 pKa = 4.56 GGTIDD250 pKa = 4.43 GGIIDD255 pKa = 4.11 TSNVIEE261 pKa = 4.33 ASIIDD266 pKa = 3.65 VDD268 pKa = 4.59 SEE270 pKa = 4.39 VSQVNNTNTGSGNVGVEE287 pKa = 4.07 SSNVEE292 pKa = 4.16 SNNVEE297 pKa = 3.85 RR298 pKa = 11.84 SNVEE302 pKa = 3.77 TSNVDD307 pKa = 3.36 VNVDD311 pKa = 3.55 AEE313 pKa = 4.35 SSNAEE318 pKa = 3.56 VDD320 pKa = 3.32 VDD322 pKa = 4.07 VDD324 pKa = 4.79 AEE326 pKa = 4.33 YY327 pKa = 10.95 TNNDD331 pKa = 3.29 DD332 pKa = 3.6 ATNVGEE338 pKa = 4.25 TTEE341 pKa = 5.8 DD342 pKa = 3.21 NTEE345 pKa = 4.06 YY346 pKa = 9.99 NTDD349 pKa = 3.1 EE350 pKa = 4.36 TTNEE354 pKa = 3.89 NTDD357 pKa = 3.28 EE358 pKa = 4.2 NTTDD362 pKa = 3.8 DD363 pKa = 4.09 TDD365 pKa = 3.07 EE366 pKa = 4.49 HH367 pKa = 6.4 TDD369 pKa = 3.31 EE370 pKa = 4.55 NTDD373 pKa = 3.25 EE374 pKa = 4.34 NTNEE378 pKa = 4.04 DD379 pKa = 3.42 TDD381 pKa = 3.94 EE382 pKa = 4.43 NTDD385 pKa = 3.08 EE386 pKa = 4.14 TTEE389 pKa = 4.17 YY390 pKa = 9.63 NTEE393 pKa = 4.47 DD394 pKa = 3.79 YY395 pKa = 11.44 NSTDD399 pKa = 3.32 VNIDD403 pKa = 2.93 ASRR406 pKa = 11.84 IEE408 pKa = 4.42 TINTSGNCFVHH419 pKa = 7.14 FLLTDD424 pKa = 3.51 TRR426 pKa = 11.84 FLNYY430 pKa = 8.52 TLSMHH435 pKa = 7.11 ASLIHH440 pKa = 6.44 IVYY443 pKa = 8.87 TCMYY447 pKa = 9.18 KK448 pKa = 10.56
Molecular weight: 49.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.743
IPC2_protein 3.846
IPC_protein 3.859
Toseland 3.656
ProMoST 3.999
Dawson 3.834
Bjellqvist 3.986
Wikipedia 3.732
Rodwell 3.681
Grimsley 3.554
Solomon 3.834
Lehninger 3.783
Nozaki 3.935
DTASelect 4.151
Thurlkill 3.681
EMBOSS 3.745
Sillero 3.973
Patrickios 1.875
IPC_peptide 3.834
IPC2_peptide 3.961
IPC2.peptide.svr19 3.875
Protein with the highest isoelectric point:
>tr|G9I0D4|G9I0D4_HZNV2 Baculovirus 38k protein OS=Helicoverpa zea nudivirus 2 OX=1128424 GN=orf108 PE=4 SV=1
MM1 pKa = 7.25 QILMRR6 pKa = 11.84 SSTYY10 pKa = 8.29 EE11 pKa = 3.55 TFMTISSFVNKK22 pKa = 10.0 RR23 pKa = 11.84 LFSTATMCDD32 pKa = 3.5 ANLSLYY38 pKa = 10.11 RR39 pKa = 11.84 VALLKK44 pKa = 10.94 KK45 pKa = 7.97 NTASVVHH52 pKa = 6.77 FIHH55 pKa = 6.14 NLSYY59 pKa = 10.85 TSTSNTTSTTTTTSTTSTTTHH80 pKa = 5.98 HH81 pKa = 7.01 RR82 pKa = 11.84 GLPILVIFQSCNINN96 pKa = 3.1
Molecular weight: 10.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.223
IPC2_protein 9.311
IPC_protein 9.472
Toseland 9.692
ProMoST 9.545
Dawson 9.984
Bjellqvist 9.78
Wikipedia 10.204
Rodwell 10.189
Grimsley 10.087
Solomon 10.072
Lehninger 10.043
Nozaki 9.853
DTASelect 9.736
Thurlkill 9.823
EMBOSS 10.116
Sillero 9.94
Patrickios 7.483
IPC_peptide 10.058
IPC2_peptide 8.916
IPC2.peptide.svr19 8.032
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
113
0
113
52527
63
1955
464.8
52.35
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.612 ± 0.274
1.959 ± 0.146
6.701 ± 0.244
6.3 ± 0.335
3.621 ± 0.14
4.245 ± 0.154
2.189 ± 0.103
5.344 ± 0.176
7.014 ± 0.299
7.828 ± 0.297
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.61 ± 0.109
6.996 ± 0.404
4.512 ± 0.221
3.115 ± 0.167
4.636 ± 0.157
9.085 ± 0.374
7.343 ± 0.355
6.509 ± 0.159
0.52 ± 0.043
3.861 ± 0.199
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here