Candidatus Syntrophoarchaeum butanivorans
Average proteome isoelectric point is 6.06
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1592 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1F2P337|A0A1F2P337_9EURY Uncharacterized protein OS=Candidatus Syntrophoarchaeum butanivorans OX=1839936 GN=SBU_001370 PE=4 SV=1
MM1 pKa = 8.01 DD2 pKa = 4.12 NFVRR6 pKa = 11.84 NSLILLVVCTAVMCVAAYY24 pKa = 10.07 AGYY27 pKa = 10.84 ASGAGMEE34 pKa = 4.34 GTDD37 pKa = 4.84 GIVEE41 pKa = 3.91 EE42 pKa = 5.11 HH43 pKa = 6.48 AASTGGLEE51 pKa = 4.88 AVDD54 pKa = 3.18 IVPNVGAFGDD64 pKa = 3.4 MGEE67 pKa = 4.39 YY68 pKa = 10.55 VGFTLAGIIAGFIFGYY84 pKa = 9.6 FWIDD88 pKa = 2.79 IKK90 pKa = 11.29 EE91 pKa = 4.41 GGGQRR96 pKa = 11.84 AA97 pKa = 3.31
Molecular weight: 9.99 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.891
IPC2_protein 4.062
IPC_protein 3.91
Toseland 3.745
ProMoST 4.075
Dawson 3.897
Bjellqvist 4.05
Wikipedia 3.821
Rodwell 3.757
Grimsley 3.656
Solomon 3.872
Lehninger 3.821
Nozaki 4.024
DTASelect 4.164
Thurlkill 3.795
EMBOSS 3.821
Sillero 4.037
Patrickios 0.401
IPC_peptide 3.872
IPC2_peptide 4.012
IPC2.peptide.svr19 3.939
Protein with the highest isoelectric point:
>tr|A0A1F2P420|A0A1F2P420_9EURY Acetoin utilization protein AcuC OS=Candidatus Syntrophoarchaeum butanivorans OX=1839936 GN=ENI32_00880 PE=4 SV=1
MM1 pKa = 7.17 GKK3 pKa = 10.23 KK4 pKa = 9.18 MKK6 pKa = 10.33 GKK8 pKa = 10.23 KK9 pKa = 9.37 LRR11 pKa = 11.84 LAKK14 pKa = 9.97 AVKK17 pKa = 9.1 QNRR20 pKa = 11.84 RR21 pKa = 11.84 VPVWVIVKK29 pKa = 7.42 TNRR32 pKa = 11.84 GVLTHH37 pKa = 6.3 PKK39 pKa = 8.75 RR40 pKa = 11.84 RR41 pKa = 11.84 NWRR44 pKa = 11.84 RR45 pKa = 11.84 SSLKK49 pKa = 10.17 VV50 pKa = 3.13
Molecular weight: 5.94 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.493
IPC2_protein 11.14
IPC_protein 12.691
Toseland 12.866
ProMoST 13.349
Dawson 12.866
Bjellqvist 12.852
Wikipedia 13.334
Rodwell 12.735
Grimsley 12.91
Solomon 13.349
Lehninger 13.261
Nozaki 12.866
DTASelect 12.852
Thurlkill 12.866
EMBOSS 13.364
Sillero 12.866
Patrickios 12.457
IPC_peptide 13.349
IPC2_peptide 12.34
IPC2.peptide.svr19 9.071
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1592
0
1592
442627
34
2514
278.0
31.13
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.028 ± 0.067
1.258 ± 0.034
5.96 ± 0.043
8.627 ± 0.06
3.738 ± 0.046
8.091 ± 0.06
1.771 ± 0.025
8.389 ± 0.05
5.465 ± 0.056
9.549 ± 0.072
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.773 ± 0.028
2.862 ± 0.04
4.125 ± 0.038
1.784 ± 0.027
6.965 ± 0.066
5.541 ± 0.046
4.616 ± 0.05
7.4 ± 0.049
0.969 ± 0.021
3.09 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here