Marinoscillum furvescens DSM 4134

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Cytophagia; Cytophagales; Reichenbachiellaceae; Marinoscillum; Marinoscillum furvescens

Average proteome isoelectric point is 6.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4702 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3D9L985|A0A3D9L985_9BACT Uncharacterized protein OS=Marinoscillum furvescens DSM 4134 OX=1122208 GN=C7460_103139 PE=4 SV=1
MM1 pKa = 7.41NNMKK5 pKa = 10.31YY6 pKa = 9.93IYY8 pKa = 10.11SALFLGLFSIALAQKK23 pKa = 9.7PIVVSLDD30 pKa = 3.28KK31 pKa = 11.46YY32 pKa = 10.68SATVDD37 pKa = 3.08EE38 pKa = 5.19TIFINGANFPTNVANVSVFFGNGKK62 pKa = 8.8ATVTSAAEE70 pKa = 3.87NQLQVTVTNTATFGPVRR87 pKa = 11.84VVNTSNGLAGASGQPFMLSYY107 pKa = 11.06SGDD110 pKa = 3.56ALDD113 pKa = 3.91ASKK116 pKa = 10.3FVKK119 pKa = 10.45RR120 pKa = 11.84STTTSEE126 pKa = 3.34QGTYY130 pKa = 10.06DD131 pKa = 3.59VCVCDD136 pKa = 5.59FNDD139 pKa = 4.3NGKK142 pKa = 10.02LDD144 pKa = 3.68AAVANFNNRR153 pKa = 11.84NISIFNNLTTFGNQASFSKK172 pKa = 10.83SSLDD176 pKa = 3.49NVSPTISVEE185 pKa = 4.05CGDD188 pKa = 4.52LNGDD192 pKa = 4.04GLPDD196 pKa = 3.78LVATSFSGSAPQHH209 pKa = 5.26VKK211 pKa = 10.34VYY213 pKa = 10.46QNNGTAGISFTKK225 pKa = 10.38VLEE228 pKa = 4.02FRR230 pKa = 11.84LPNLSASVPRR240 pKa = 11.84NPRR243 pKa = 11.84KK244 pKa = 9.81IKK246 pKa = 10.31IADD249 pKa = 3.4MDD251 pKa = 4.63LDD253 pKa = 4.23GKK255 pKa = 10.98SDD257 pKa = 4.76LIIGSEE263 pKa = 3.97NDD265 pKa = 3.06NSIFIYY271 pKa = 10.81QNTTTGGTLSFASSPEE287 pKa = 4.17SITVSGADD295 pKa = 3.07NAASIEE301 pKa = 4.08IGDD304 pKa = 4.53LNNDD308 pKa = 3.9GLPDD312 pKa = 3.62IAAVALNIPNSSVFVIRR329 pKa = 11.84NQSSPGSFNFIQASTINDD347 pKa = 3.34SNQRR351 pKa = 11.84TNVSLVDD358 pKa = 3.21IDD360 pKa = 5.34GDD362 pKa = 4.06FKK364 pKa = 11.58PEE366 pKa = 4.19IITTNNLKK374 pKa = 10.45NDD376 pKa = 2.82VDD378 pKa = 3.5IFEE381 pKa = 4.67NTTSGIEE388 pKa = 3.74ITFDD392 pKa = 3.37NSPTTLSVQRR402 pKa = 11.84PWSVASGDD410 pKa = 3.79LNGDD414 pKa = 3.58GKK416 pKa = 11.39VDD418 pKa = 4.01LSVATLSADD427 pKa = 2.95IYY429 pKa = 10.64VLEE432 pKa = 4.4NTTSNGSIAFTSSTFSATPDD452 pKa = 3.12NRR454 pKa = 11.84NIEE457 pKa = 4.06LADD460 pKa = 3.36IDD462 pKa = 4.99GDD464 pKa = 3.96AKK466 pKa = 10.86PDD468 pKa = 3.73VITTNNTGEE477 pKa = 4.27TATGSLLVMLNQNCTVSKK495 pKa = 10.49ILPEE499 pKa = 4.59DD500 pKa = 3.21LTFCLNEE507 pKa = 4.87PITLRR512 pKa = 11.84ATNTRR517 pKa = 11.84NATYY521 pKa = 10.16SWAITSGNGNITGSGQEE538 pKa = 3.89VQVTVTSGEE547 pKa = 4.21TATVQVTTAANDD559 pKa = 4.39GSCSEE564 pKa = 4.63ASSQVFSLTGGTPPPTPSISNSASGTICSGDD595 pKa = 3.76SFTLTASTATADD607 pKa = 3.35EE608 pKa = 5.13YY609 pKa = 11.79YY610 pKa = 9.83WVTPSGDD617 pKa = 3.62QITTTTNTLEE627 pKa = 4.19FSDD630 pKa = 4.74VSSADD635 pKa = 3.01AGNYY639 pKa = 6.2TVRR642 pKa = 11.84TFTSGSCTSAEE653 pKa = 4.25SPALTVAIDD662 pKa = 3.79EE663 pKa = 4.78PPSVVIASSASTTFCANSTINLIVPDD689 pKa = 4.1YY690 pKa = 11.21DD691 pKa = 3.57GYY693 pKa = 11.22SYY695 pKa = 10.9QWHH698 pKa = 7.25KK699 pKa = 11.42DD700 pKa = 3.29GTDD703 pKa = 3.54LTGEE707 pKa = 4.1TANTFTANASGSYY720 pKa = 9.12TVTLTSNATSCSKK733 pKa = 10.02TSGAIALTAVEE744 pKa = 4.73LPVGNIASEE753 pKa = 4.88DD754 pKa = 4.09EE755 pKa = 4.09ICVNAEE761 pKa = 3.42ISFDD765 pKa = 3.82GSGSTSDD772 pKa = 3.69PNGEE776 pKa = 4.24LTYY779 pKa = 11.0SWDD782 pKa = 3.73FGDD785 pKa = 5.41GNTADD790 pKa = 4.13TEE792 pKa = 4.79TANHH796 pKa = 7.08TYY798 pKa = 7.88TTDD801 pKa = 2.9GSYY804 pKa = 9.46TVTLTTGYY812 pKa = 11.01SNVNSCEE819 pKa = 3.87STTQKK824 pKa = 9.43TVEE827 pKa = 4.02IAALPSNDD835 pKa = 3.98EE836 pKa = 3.74IAAALTPDD844 pKa = 3.62PSTTQKK850 pKa = 10.72CPEE853 pKa = 4.54DD854 pKa = 4.1SLTLSLPTNYY864 pKa = 9.87LAYY867 pKa = 9.65EE868 pKa = 4.03WTVDD872 pKa = 3.19GDD874 pKa = 4.31VVSTTNSAKK883 pKa = 10.38IGTAPKK889 pKa = 10.39SSSTDD894 pKa = 2.98VTIDD898 pKa = 3.07VTTDD902 pKa = 3.48LGCVVDD908 pKa = 3.77ATVITVSNLANAGFTFSSPDD928 pKa = 3.32AQIVNDD934 pKa = 5.2TITLDD939 pKa = 3.5DD940 pKa = 3.62RR941 pKa = 11.84TAEE944 pKa = 4.06VTLQVSNGTDD954 pKa = 3.96FTWSPEE960 pKa = 3.92EE961 pKa = 4.41VISSTSGEE969 pKa = 4.35MIDD972 pKa = 3.59VYY974 pKa = 10.82PRR976 pKa = 11.84SQYY979 pKa = 8.05TTVTVTGTDD988 pKa = 3.38TEE990 pKa = 4.94GCTNTSQITIVSPGLIPRR1008 pKa = 11.84KK1009 pKa = 9.8SFSPNGDD1016 pKa = 3.58GIGYY1020 pKa = 7.54EE1021 pKa = 4.06CWEE1024 pKa = 4.17ILNSEE1029 pKa = 4.53SLTGCTIFILDD1040 pKa = 3.46QKK1042 pKa = 10.72GSHH1045 pKa = 5.19VLKK1048 pKa = 10.98AEE1050 pKa = 3.92APFVDD1055 pKa = 3.24NCVWNGNIDD1064 pKa = 3.69NGTSPVPEE1072 pKa = 3.76GVYY1075 pKa = 10.53YY1076 pKa = 10.62YY1077 pKa = 10.11ILKK1080 pKa = 10.38CDD1082 pKa = 4.47DD1083 pKa = 4.37GADD1086 pKa = 3.79SSTGTIMLARR1096 pKa = 4.46

Molecular weight: 114.93 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3D9L4V2|A0A3D9L4V2_9BACT Uridylate kinase OS=Marinoscillum furvescens DSM 4134 OX=1122208 GN=pyrH PE=3 SV=1
MM1 pKa = 7.82PLFYY5 pKa = 10.22WCALVFKK12 pKa = 10.4LRR14 pKa = 11.84TGLEE18 pKa = 4.0DD19 pKa = 3.36TFVKK23 pKa = 10.26IFPVAAIFRR32 pKa = 11.84VNAATVRR39 pKa = 11.84PNAGVHH45 pKa = 5.85PMNAAIPHH53 pKa = 6.17INAEE57 pKa = 4.25TFRR60 pKa = 11.84ISAIIVRR67 pKa = 11.84VNAEE71 pKa = 4.22TLRR74 pKa = 11.84MNAAIVRR81 pKa = 11.84PNTVVHH87 pKa = 6.42PMNVAIPHH95 pKa = 6.26INAEE99 pKa = 4.37TFRR102 pKa = 11.84MNAIAVHH109 pKa = 5.93VNAEE113 pKa = 4.3TLRR116 pKa = 11.84MNAAIFRR123 pKa = 11.84ISSTIGLSEE132 pKa = 3.93

Molecular weight: 14.59 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4702

0

4702

1798820

29

6013

382.6

42.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.379 ± 0.035

0.724 ± 0.01

5.982 ± 0.043

6.613 ± 0.034

4.667 ± 0.026

7.188 ± 0.041

2.071 ± 0.019

6.363 ± 0.03

5.736 ± 0.042

9.413 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.227 ± 0.016

4.967 ± 0.033

3.892 ± 0.022

3.922 ± 0.022

4.132 ± 0.029

6.724 ± 0.037

5.872 ± 0.048

6.673 ± 0.028

1.358 ± 0.014

4.097 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski