Flavobacteriaceae bacterium MAR_2010_72

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; unclassified Flavobacteriaceae

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2943 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A397L7I9|A0A397L7I9_9FLAO Uncharacterized protein OS=Flavobacteriaceae bacterium MAR_2010_72 OX=1250029 GN=OE09_0180 PE=4 SV=1
MM1 pKa = 7.57KK2 pKa = 10.05SIKK5 pKa = 10.04ILTVLILALIGFNSCEE21 pKa = 3.96KK22 pKa = 10.95DD23 pKa = 3.32EE24 pKa = 5.02LVFTAQEE31 pKa = 3.81QGEE34 pKa = 4.5FTFTNTFLAQYY45 pKa = 9.45ILTSTASANIGEE57 pKa = 4.54RR58 pKa = 11.84FTWTSVNFDD67 pKa = 3.3VPTAVSYY74 pKa = 10.82DD75 pKa = 3.72LQGSISGDD83 pKa = 3.47FTDD86 pKa = 3.65MTVIGSTSGNDD97 pKa = 2.97IAVTIGKK104 pKa = 9.06LMSMATTAGLDD115 pKa = 3.34NDD117 pKa = 4.97PNTEE121 pKa = 4.0NPNTGQLFFRR131 pKa = 11.84LRR133 pKa = 11.84AYY135 pKa = 10.28AGNDD139 pKa = 3.45AIEE142 pKa = 4.47TLSSVQSLTVVLPEE156 pKa = 4.03IVDD159 pKa = 3.6TGGSGITVSNWGVVGSAYY177 pKa = 10.64NDD179 pKa = 3.3WGNAGLDD186 pKa = 3.45GAFYY190 pKa = 9.87STNNPDD196 pKa = 3.61VIVSYY201 pKa = 9.63VTLLAGAMKK210 pKa = 10.25FRR212 pKa = 11.84LDD214 pKa = 3.89NDD216 pKa = 3.1WTTNYY221 pKa = 10.05GDD223 pKa = 3.71NGADD227 pKa = 3.43GTLEE231 pKa = 3.97EE232 pKa = 4.89GGADD236 pKa = 2.71IVVTAGTYY244 pKa = 10.28KK245 pKa = 9.45ITVDD249 pKa = 3.92TNAKK253 pKa = 7.97TYY255 pKa = 9.92TMEE258 pKa = 4.73PYY260 pKa = 10.31AWGIVGSAYY269 pKa = 10.69NDD271 pKa = 3.38WGNAGPDD278 pKa = 2.96AKK280 pKa = 10.54LYY282 pKa = 10.83YY283 pKa = 10.35DD284 pKa = 3.67YY285 pKa = 8.48TTDD288 pKa = 3.27TFKK291 pKa = 11.38VGVKK295 pKa = 10.5LLDD298 pKa = 3.54GAMKK302 pKa = 10.3FRR304 pKa = 11.84FNNDD308 pKa = 1.68WTTNYY313 pKa = 10.41GDD315 pKa = 3.63TGADD319 pKa = 3.33GSLDD323 pKa = 3.59EE324 pKa = 5.49GGDD327 pKa = 4.06DD328 pKa = 3.94IIVTAGHH335 pKa = 5.78YY336 pKa = 10.74NITFDD341 pKa = 4.71LNNGEE346 pKa = 4.15YY347 pKa = 10.09SIEE350 pKa = 4.08SAEE353 pKa = 3.92VLGIVGSAYY362 pKa = 10.71NDD364 pKa = 3.52WGNGGPDD371 pKa = 3.58FSLTQVSPDD380 pKa = 3.35IYY382 pKa = 11.04VGDD385 pKa = 3.83IATLIGGAMKK395 pKa = 10.34FRR397 pKa = 11.84VNNDD401 pKa = 1.96WTTNYY406 pKa = 10.41GDD408 pKa = 3.59TGADD412 pKa = 3.31GVLDD416 pKa = 4.21AGGDD420 pKa = 4.14DD421 pKa = 3.45IVVTAGKK428 pKa = 10.02YY429 pKa = 8.44RR430 pKa = 11.84VRR432 pKa = 11.84IDD434 pKa = 3.83LTDD437 pKa = 3.42GSYY440 pKa = 9.53TLNKK444 pKa = 9.24IQQ446 pKa = 4.41

Molecular weight: 47.64 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A397LES0|A0A397LES0_9FLAO O-antigen ligase-like membrane protein OS=Flavobacteriaceae bacterium MAR_2010_72 OX=1250029 GN=OE09_2104 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.01RR4 pKa = 11.84TFQPSKK10 pKa = 9.13RR11 pKa = 11.84KK12 pKa = 9.48RR13 pKa = 11.84RR14 pKa = 11.84NKK16 pKa = 9.49HH17 pKa = 3.94GFRR20 pKa = 11.84EE21 pKa = 4.19RR22 pKa = 11.84MATANGRR29 pKa = 11.84KK30 pKa = 8.93VLARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.09GRR40 pKa = 11.84KK41 pKa = 8.0KK42 pKa = 10.65LSVSTEE48 pKa = 3.95SRR50 pKa = 11.84HH51 pKa = 6.01KK52 pKa = 10.6KK53 pKa = 9.6

Molecular weight: 6.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2943

0

2943

1037183

30

4502

352.4

39.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.467 ± 0.049

0.785 ± 0.026

5.808 ± 0.062

6.218 ± 0.048

5.154 ± 0.039

6.482 ± 0.053

1.867 ± 0.023

7.875 ± 0.037

7.301 ± 0.067

9.329 ± 0.061

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.154 ± 0.023

6.254 ± 0.046

3.476 ± 0.029

3.473 ± 0.026

3.362 ± 0.028

6.567 ± 0.039

6.021 ± 0.073

6.273 ± 0.034

1.088 ± 0.016

4.046 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski