Torque teno tamarin virus (isolate So-TTV2)
Average proteome isoelectric point is 7.12
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|Q9DUC2|ORF2_TTVE1 Uncharacterized ORF2 protein OS=Torque teno tamarin virus (isolate So-TTV2) OX=766186 GN=ORF2 PE=4 SV=1
MM1 pKa = 7.29 QRR3 pKa = 11.84 RR4 pKa = 11.84 TQEE7 pKa = 4.62 LIWKK11 pKa = 8.36 EE12 pKa = 3.83 LVDD15 pKa = 3.81 NSHH18 pKa = 7.26 KK19 pKa = 10.77 LFCNCMDD26 pKa = 4.01 PQNHH30 pKa = 5.59 YY31 pKa = 11.05 RR32 pKa = 11.84 LICQNLNRR40 pKa = 11.84 EE41 pKa = 4.19 PGEE44 pKa = 3.8 PWRR47 pKa = 11.84 TAGGIGEE54 pKa = 4.63 GGAGGDD60 pKa = 3.54 GAAAGGEE67 pKa = 4.29 GDD69 pKa = 3.77 VHH71 pKa = 6.12 GRR73 pKa = 11.84 PAGAEE78 pKa = 3.99 DD79 pKa = 4.72 GEE81 pKa = 5.3 DD82 pKa = 3.66 GADD85 pKa = 3.23 AAMAAALAAFEE96 pKa = 4.87 DD97 pKa = 4.23 ATGG100 pKa = 3.26
Molecular weight: 10.42 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.542
IPC2_protein 4.546
IPC_protein 4.431
Toseland 4.279
ProMoST 4.546
Dawson 4.393
Bjellqvist 4.533
Wikipedia 4.266
Rodwell 4.279
Grimsley 4.19
Solomon 4.38
Lehninger 4.342
Nozaki 4.507
DTASelect 4.66
Thurlkill 4.291
EMBOSS 4.291
Sillero 4.546
Patrickios 3.783
IPC_peptide 4.393
IPC2_peptide 4.546
IPC2.peptide.svr19 4.477
Protein with the highest isoelectric point:
>sp|Q9DUC2|ORF2_TTVE1 Uncharacterized ORF2 protein OS=Torque teno tamarin virus (isolate So-TTV2) OX=766186 GN=ORF2 PE=4 SV=1
MM1 pKa = 7.89 AYY3 pKa = 9.97 GRR5 pKa = 11.84 RR6 pKa = 11.84 YY7 pKa = 6.99 WRR9 pKa = 11.84 RR10 pKa = 11.84 RR11 pKa = 11.84 RR12 pKa = 11.84 WRR14 pKa = 11.84 RR15 pKa = 11.84 RR16 pKa = 11.84 GGRR19 pKa = 11.84 WWRR22 pKa = 11.84 RR23 pKa = 11.84 RR24 pKa = 11.84 RR25 pKa = 11.84 PWKK28 pKa = 8.65 TRR30 pKa = 11.84 WRR32 pKa = 11.84 RR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 RR37 pKa = 11.84 WRR39 pKa = 11.84 RR40 pKa = 11.84 RR41 pKa = 11.84 RR42 pKa = 11.84 YY43 pKa = 8.87 GRR45 pKa = 11.84 RR46 pKa = 11.84 ARR48 pKa = 11.84 RR49 pKa = 11.84 FRR51 pKa = 11.84 RR52 pKa = 11.84 RR53 pKa = 11.84 HH54 pKa = 4.67 RR55 pKa = 11.84 VGRR58 pKa = 11.84 WRR60 pKa = 11.84 KK61 pKa = 8.63 LFRR64 pKa = 11.84 RR65 pKa = 11.84 VKK67 pKa = 10.42 SRR69 pKa = 11.84 VVRR72 pKa = 11.84 QWDD75 pKa = 3.5 PNVTRR80 pKa = 11.84 RR81 pKa = 11.84 CMINGYY87 pKa = 9.56 DD88 pKa = 3.59 HH89 pKa = 7.21 ALFWGQQAQHH99 pKa = 6.95 RR100 pKa = 11.84 ILYY103 pKa = 10.26 DD104 pKa = 3.46 NLPWPIKK111 pKa = 10.56 KK112 pKa = 10.25 EE113 pKa = 4.01 GTQEE117 pKa = 4.05 GGSMNMMQLTLQFLYY132 pKa = 10.67 KK133 pKa = 10.75 DD134 pKa = 3.51 NLAGRR139 pKa = 11.84 NRR141 pKa = 11.84 WTRR144 pKa = 11.84 SNWDD148 pKa = 3.03 MDD150 pKa = 3.79 LAKK153 pKa = 10.78 YY154 pKa = 10.56 HH155 pKa = 5.9 EE156 pKa = 4.56 TTLLFPRR163 pKa = 11.84 HH164 pKa = 5.88 RR165 pKa = 11.84 NYY167 pKa = 11.02 SYY169 pKa = 11.46 VVFITRR175 pKa = 11.84 GPNDD179 pKa = 3.4 ILDD182 pKa = 3.54 EE183 pKa = 4.09 HH184 pKa = 6.88 TYY186 pKa = 10.65 PGLHH190 pKa = 6.53 PEE192 pKa = 3.96 KK193 pKa = 10.23 MLGRR197 pKa = 11.84 RR198 pKa = 11.84 KK199 pKa = 10.29 KK200 pKa = 9.95 IVVWSKK206 pKa = 10.68 DD207 pKa = 3.48 LRR209 pKa = 11.84 PHH211 pKa = 6.03 GKK213 pKa = 8.75 NYY215 pKa = 9.64 IRR217 pKa = 11.84 VRR219 pKa = 11.84 VPPPQVFKK227 pKa = 10.84 NQWYY231 pKa = 7.66 FQRR234 pKa = 11.84 DD235 pKa = 3.33 ICQMPILTLGFAAFDD250 pKa = 3.93 PVNVTLAGNMANSSIILWGIPYY272 pKa = 9.5 WSRR275 pKa = 11.84 PMPYY279 pKa = 8.55 NTWYY283 pKa = 10.23 DD284 pKa = 3.54 YY285 pKa = 7.61 WTKK288 pKa = 10.7 CAGPQNIPWSDD299 pKa = 3.22 RR300 pKa = 11.84 QTNIAAGITQKK311 pKa = 10.73 SAQTCLKK318 pKa = 10.32 LKK320 pKa = 10.2 NKK322 pKa = 10.0 HH323 pKa = 6.3 FGLTNTAIVSDD334 pKa = 4.09 YY335 pKa = 11.64 DD336 pKa = 4.16 KK337 pKa = 11.8 DD338 pKa = 4.79 NIDD341 pKa = 4.61 KK342 pKa = 8.75 NTKK345 pKa = 8.41 QIKK348 pKa = 8.14 TIVMSLNRR356 pKa = 11.84 WYY358 pKa = 9.95 PKK360 pKa = 10.34 WPMKK364 pKa = 10.15 WKK366 pKa = 10.38 DD367 pKa = 3.21 AGDD370 pKa = 3.87 INTQTPFPYY379 pKa = 9.62 RR380 pKa = 11.84 YY381 pKa = 9.37 SWRR384 pKa = 11.84 EE385 pKa = 3.58 DD386 pKa = 3.27 QGWGNKK392 pKa = 6.97 VRR394 pKa = 11.84 LWTRR398 pKa = 11.84 EE399 pKa = 3.69 CRR401 pKa = 11.84 TDD403 pKa = 3.36 IPEE406 pKa = 4.01 EE407 pKa = 3.84 TLGIEE412 pKa = 4.19 NMPLYY417 pKa = 11.12 VLMNGYY423 pKa = 9.4 IDD425 pKa = 4.31 YY426 pKa = 8.66 VTNHH430 pKa = 5.52 STHH433 pKa = 6.59 SPLNWVVSVFCPYY446 pKa = 10.16 TDD448 pKa = 3.69 PPMTNVIPVGKK459 pKa = 9.79 DD460 pKa = 2.65 WFIQNVEE467 pKa = 4.11 PGEE470 pKa = 4.05 NKK472 pKa = 10.25 YY473 pKa = 10.83 PSDD476 pKa = 3.59 NADD479 pKa = 3.07 NTFAPGEE486 pKa = 3.99 KK487 pKa = 9.43 TKK489 pKa = 10.62 IKK491 pKa = 10.73 NYY493 pKa = 10.24 DD494 pKa = 3.23 SSKK497 pKa = 11.51 DD498 pKa = 3.51 EE499 pKa = 3.86 FTGNVIRR506 pKa = 11.84 APDD509 pKa = 3.68 VYY511 pKa = 11.17 DD512 pKa = 4.13 SLPAQQALYY521 pKa = 9.65 IASPFSLKK529 pKa = 9.23 WAQTTGSIVFFYY541 pKa = 10.06 QSKK544 pKa = 7.01 WTWGGDD550 pKa = 3.79 FPRR553 pKa = 11.84 QRR555 pKa = 11.84 PIIDD559 pKa = 3.41 PCNRR563 pKa = 11.84 PKK565 pKa = 10.41 WGGLPVTGYY574 pKa = 11.11 DD575 pKa = 3.38 EE576 pKa = 5.02 TGLLLQNPEE585 pKa = 3.92 KK586 pKa = 11.11 AEE588 pKa = 3.77 ARR590 pKa = 11.84 ARR592 pKa = 11.84 GHH594 pKa = 7.04 LGDD597 pKa = 4.78 LRR599 pKa = 11.84 RR600 pKa = 11.84 GDD602 pKa = 3.74 LTKK605 pKa = 10.22 TALKK609 pKa = 10.51 RR610 pKa = 11.84 LMEE613 pKa = 4.17 LTSTEE618 pKa = 3.92 EE619 pKa = 4.2 GSPQKK624 pKa = 10.45 KK625 pKa = 9.23 KK626 pKa = 10.76 RR627 pKa = 11.84 RR628 pKa = 11.84 DD629 pKa = 3.27 KK630 pKa = 10.8 EE631 pKa = 3.99 VRR633 pKa = 11.84 TTADD637 pKa = 3.43 LLRR640 pKa = 11.84 PEE642 pKa = 6.0 DD643 pKa = 4.21 VYY645 pKa = 11.76 CWMSPTPEE653 pKa = 4.18 TPPKK657 pKa = 10.29 AGKK660 pKa = 8.13 TPEE663 pKa = 3.82 SSFAEE668 pKa = 4.67 TYY670 pKa = 10.91 DD671 pKa = 3.41 NHH673 pKa = 6.02 TKK675 pKa = 10.03 LAKK678 pKa = 9.58 RR679 pKa = 11.84 VKK681 pKa = 10.61 KK682 pKa = 10.17 EE683 pKa = 3.16 IHH685 pKa = 5.25 NQNRR689 pKa = 11.84 RR690 pKa = 11.84 HH691 pKa = 6.04 RR692 pKa = 11.84 LLLAHH697 pKa = 7.2 LRR699 pKa = 11.84 RR700 pKa = 11.84 EE701 pKa = 3.92 RR702 pKa = 11.84 DD703 pKa = 3.22 RR704 pKa = 11.84 LLGHH708 pKa = 6.66 HH709 pKa = 7.0 LLRR712 pKa = 6.03
Molecular weight: 84.93 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.319
IPC2_protein 9.706
IPC_protein 10.35
Toseland 10.496
ProMoST 10.204
Dawson 10.643
Bjellqvist 10.335
Wikipedia 10.833
Rodwell 10.935
Grimsley 10.701
Solomon 10.701
Lehninger 10.657
Nozaki 10.482
DTASelect 10.321
Thurlkill 10.511
EMBOSS 10.891
Sillero 10.555
Patrickios 10.57
IPC_peptide 10.701
IPC2_peptide 9.194
IPC2.peptide.svr19 8.668
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
1041
70
712
260.3
30.54
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.572 ± 2.285
1.345 ± 0.392
5.86 ± 0.48
4.803 ± 1.394
2.498 ± 0.739
6.82 ± 2.189
2.498 ± 0.441
3.554 ± 1.147
6.34 ± 1.154
6.82 ± 0.606
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.209 ± 0.433
4.803 ± 1.07
7.493 ± 1.303
3.65 ± 0.311
11.72 ± 2.387
4.899 ± 1.643
7.685 ± 1.835
3.362 ± 1.392
4.227 ± 0.999
3.842 ± 0.992
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here