Corynebacterium imitans
Average proteome isoelectric point is 5.79
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2011 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A076NIH4|A0A076NIH4_9CORY Phosphotransacetylase OS=Corynebacterium imitans OX=156978 GN=eutD PE=4 SV=1
MM1 pKa = 7.37 GKK3 pKa = 10.51 KK4 pKa = 8.89 MLPVSLGLAAAVLAGCASDD23 pKa = 3.75 PVEE26 pKa = 4.29 PAAEE30 pKa = 4.2 VAEE33 pKa = 4.39 PEE35 pKa = 3.95 APAFHH40 pKa = 6.77 FASGTLEE47 pKa = 5.09 LGDD50 pKa = 4.87 FDD52 pKa = 5.5 PATLGDD58 pKa = 5.13 DD59 pKa = 4.74 LFDD62 pKa = 4.3 PCAEE66 pKa = 4.02 ISAEE70 pKa = 4.08 EE71 pKa = 3.95 YY72 pKa = 10.61 AAAGMTGVEE81 pKa = 4.36 PEE83 pKa = 4.02 PVIAEE88 pKa = 4.22 SSNGLALGCDD98 pKa = 4.06 SDD100 pKa = 5.53 PIDD103 pKa = 3.83 HH104 pKa = 7.01 GIDD107 pKa = 3.14 RR108 pKa = 11.84 SIVSSRR114 pKa = 11.84 TPAAGVSEE122 pKa = 4.21 TAGYY126 pKa = 9.02 EE127 pKa = 3.68 LSYY130 pKa = 11.16 PEE132 pKa = 4.49 TEE134 pKa = 4.41 VPDD137 pKa = 4.14 AYY139 pKa = 11.22 LMKK142 pKa = 10.62 NPVVSPYY149 pKa = 10.88 LCAAQVDD156 pKa = 4.43 TTRR159 pKa = 11.84 GALGVSVSVSGIKK172 pKa = 9.97 KK173 pKa = 9.97 EE174 pKa = 4.55 SIDD177 pKa = 3.75 ACVLALDD184 pKa = 3.95 NLEE187 pKa = 4.33 RR188 pKa = 11.84 LYY190 pKa = 10.87 KK191 pKa = 10.83 ASGQEE196 pKa = 3.83 AAEE199 pKa = 4.22 EE200 pKa = 4.22 MM201 pKa = 4.42
Molecular weight: 20.64 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.781
IPC2_protein 3.948
IPC_protein 3.91
Toseland 3.719
ProMoST 4.037
Dawson 3.872
Bjellqvist 4.024
Wikipedia 3.757
Rodwell 3.745
Grimsley 3.63
Solomon 3.859
Lehninger 3.821
Nozaki 3.986
DTASelect 4.139
Thurlkill 3.745
EMBOSS 3.77
Sillero 4.024
Patrickios 1.888
IPC_peptide 3.859
IPC2_peptide 3.999
IPC2.peptide.svr19 3.918
Protein with the highest isoelectric point:
>tr|A0A076NK21|A0A076NK21_9CORY Ribonuclease J OS=Corynebacterium imitans OX=156978 GN=rnj PE=3 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.0 RR4 pKa = 11.84 TFQPNNRR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 ARR15 pKa = 11.84 KK16 pKa = 8.24 HH17 pKa = 4.59 GFRR20 pKa = 11.84 ARR22 pKa = 11.84 MRR24 pKa = 11.84 TRR26 pKa = 11.84 AGRR29 pKa = 11.84 AIVSARR35 pKa = 11.84 RR36 pKa = 11.84 KK37 pKa = 9.62 KK38 pKa = 10.53 GRR40 pKa = 11.84 AKK42 pKa = 10.73 LSAA45 pKa = 3.92
Molecular weight: 5.29 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.316
IPC_protein 12.925
Toseland 13.086
ProMoST 13.583
Dawson 13.086
Bjellqvist 13.086
Wikipedia 13.568
Rodwell 12.735
Grimsley 13.13
Solomon 13.583
Lehninger 13.481
Nozaki 13.086
DTASelect 13.086
Thurlkill 13.086
EMBOSS 13.583
Sillero 13.086
Patrickios 12.457
IPC_peptide 13.583
IPC2_peptide 12.574
IPC2.peptide.svr19 9.237
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2011
0
2011
654327
40
3024
325.4
35.22
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.259 ± 0.08
0.672 ± 0.014
6.206 ± 0.05
6.831 ± 0.051
3.246 ± 0.035
8.375 ± 0.048
2.093 ± 0.026
4.678 ± 0.038
3.274 ± 0.052
9.409 ± 0.068
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.171 ± 0.028
2.781 ± 0.038
5.081 ± 0.04
3.306 ± 0.03
6.279 ± 0.058
5.221 ± 0.034
6.18 ± 0.049
8.302 ± 0.049
1.346 ± 0.02
2.291 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here