Marseillevirus marseillevirus (GBM)

Taxonomy: Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Megaviricetes; Pimascovirales; Marseilleviridae; Marseillevirus

Average proteome isoelectric point is 7.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 428 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D2XA93|D2XA93_GBMV Uncharacterized protein OS=Marseillevirus marseillevirus OX=694581 GN=MAR_ORF085 PE=4 SV=1
MM1 pKa = 7.16SLVRR5 pKa = 11.84GFFKK9 pKa = 10.57DD10 pKa = 3.24IPIVGSYY17 pKa = 10.72SVLPPVLPRR26 pKa = 11.84GSLVFLTTDD35 pKa = 2.98QNLYY39 pKa = 10.15ISNGQIWAVAGGDD52 pKa = 3.84VGPLAAQVAQNTADD66 pKa = 3.38IATLQTDD73 pKa = 3.63VTTLDD78 pKa = 3.56GQVATNTSNITALQGQVATNTSDD101 pKa = 2.77IGTLQGQVATNTTDD115 pKa = 2.52ITTLQGQVATNTSNITTLQGQVATNTSDD143 pKa = 2.76ITSNTNSINNIIVSQSQGFTIADD166 pKa = 3.64SAQLRR171 pKa = 11.84TFTNPITRR179 pKa = 11.84IDD181 pKa = 3.71VSSTSPTVIYY191 pKa = 10.0TLGTPLDD198 pKa = 3.85GSFNGVYY205 pKa = 10.54LIQWNILAHH214 pKa = 5.22YY215 pKa = 7.21QTANVFYY222 pKa = 10.62AVGQSSVLSNGIGALVSVFGTSTNQTNSSGSSVITVTADD261 pKa = 2.85INLNNYY267 pKa = 9.39RR268 pKa = 11.84LIVTSNSAVTTTYY281 pKa = 10.04SGYY284 pKa = 10.92VIVTRR289 pKa = 11.84ILTISPP295 pKa = 3.78

Molecular weight: 30.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D2XB80|D2XB80_GBMV Ankyrin repeat-containing protein OS=Marseillevirus marseillevirus OX=694581 GN=MAR_ORF445 PE=4 SV=1
MM1 pKa = 7.64EE2 pKa = 4.99FFGVNIFKK10 pKa = 10.22RR11 pKa = 11.84QKK13 pKa = 8.88IFEE16 pKa = 4.34SSVVANIWSFYY27 pKa = 9.64FYY29 pKa = 10.75EE30 pKa = 4.22HH31 pKa = 7.0AVRR34 pKa = 11.84SARR37 pKa = 11.84RR38 pKa = 11.84CDD40 pKa = 3.5VEE42 pKa = 4.21EE43 pKa = 4.03LLEE46 pKa = 4.35VGSRR50 pKa = 11.84RR51 pKa = 11.84WKK53 pKa = 9.99RR54 pKa = 11.84NEE56 pKa = 3.66AKK58 pKa = 10.3FPRR61 pKa = 11.84LFRR64 pKa = 11.84VQQRR68 pKa = 11.84QRR70 pKa = 11.84NPDD73 pKa = 3.47RR74 pKa = 11.84EE75 pKa = 4.31FSASPRR81 pKa = 11.84ASRR84 pKa = 11.84GIQGSRR90 pKa = 11.84HH91 pKa = 5.34RR92 pKa = 11.84RR93 pKa = 11.84PEE95 pKa = 3.33RR96 pKa = 11.84HH97 pKa = 5.01FCVFRR102 pKa = 11.84EE103 pKa = 3.95HH104 pKa = 6.21LQAPFEE110 pKa = 4.53GEE112 pKa = 3.64DD113 pKa = 3.38AQRR116 pKa = 11.84IGDD119 pKa = 3.84FGKK122 pKa = 9.86HH123 pKa = 4.4SRR125 pKa = 11.84KK126 pKa = 8.55TGEE129 pKa = 3.69TAKK132 pKa = 11.06NKK134 pKa = 9.27GAKK137 pKa = 9.09SRR139 pKa = 11.84NPLSSGCDD147 pKa = 3.14ARR149 pKa = 11.84KK150 pKa = 7.79TRR152 pKa = 11.84RR153 pKa = 11.84NDD155 pKa = 3.69ALNTDD160 pKa = 3.89NPDD163 pKa = 3.41SFRR166 pKa = 11.84SAALKK171 pKa = 10.68YY172 pKa = 10.53FEE174 pKa = 4.58

Molecular weight: 20.44 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

428

0

428

102414

52

1537

239.3

27.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.135 ± 0.124

2.39 ± 0.11

4.619 ± 0.073

9.039 ± 0.189

5.765 ± 0.095

6.357 ± 0.119

1.825 ± 0.07

4.802 ± 0.073

8.809 ± 0.199

8.892 ± 0.114

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.068 ± 0.05

3.726 ± 0.099

3.992 ± 0.087

3.364 ± 0.084

5.856 ± 0.11

7.643 ± 0.168

4.851 ± 0.119

6.354 ± 0.09

1.582 ± 0.057

2.933 ± 0.067
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski