Lachnoclostridium sp. An196

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; Lachnoclostridium; unclassified Lachnoclostridium

Average proteome isoelectric point is 5.96

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3081 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Y4IPK1|A0A1Y4IPK1_9FIRM Probable cell division protein WhiA OS=Lachnoclostridium sp. An196 OX=1965583 GN=whiA PE=3 SV=1
MM1 pKa = 7.13KK2 pKa = 10.43RR3 pKa = 11.84KK4 pKa = 9.15LAVMAMAGVLAVSAVACGSSTTSTDD29 pKa = 3.38SADD32 pKa = 3.56TATEE36 pKa = 4.01STDD39 pKa = 3.28AAEE42 pKa = 4.53EE43 pKa = 4.3STDD46 pKa = 3.51AAAADD51 pKa = 4.0SASSGEE57 pKa = 4.22VANADD62 pKa = 4.03KK63 pKa = 10.82PLVWFNRR70 pKa = 11.84QPSNSSTGEE79 pKa = 3.63LDD81 pKa = 3.17MAALNFNEE89 pKa = 3.6NTYY92 pKa = 11.09YY93 pKa = 11.02VGFDD97 pKa = 3.41ANQGAEE103 pKa = 4.18LQGTMVLDD111 pKa = 3.99YY112 pKa = 10.96IEE114 pKa = 5.64ANIDD118 pKa = 3.7SIDD121 pKa = 3.8RR122 pKa = 11.84NGDD125 pKa = 3.54GIIGYY130 pKa = 9.13VLAIGDD136 pKa = 3.91IGHH139 pKa = 6.8NDD141 pKa = 3.2SIARR145 pKa = 11.84TRR147 pKa = 11.84GVRR150 pKa = 11.84SALGTGVDD158 pKa = 3.84ADD160 pKa = 3.82GSINSEE166 pKa = 3.81PVGTNTDD173 pKa = 3.2GSSSVVQDD181 pKa = 2.87GTIEE185 pKa = 4.29VNGQTYY191 pKa = 7.35TVRR194 pKa = 11.84EE195 pKa = 4.1LASQEE200 pKa = 4.09MKK202 pKa = 10.84NSAGATWDD210 pKa = 3.21AATAGNAINTWSASFGDD227 pKa = 3.84QIDD230 pKa = 3.96VVVSNNDD237 pKa = 3.14GMGMSMFNAWSKK249 pKa = 11.45DD250 pKa = 3.24NSVPTFGYY258 pKa = 10.14DD259 pKa = 3.22ANSDD263 pKa = 3.32AVAAIAEE270 pKa = 4.99GYY272 pKa = 10.39GGTISQHH279 pKa = 6.65ADD281 pKa = 2.86VQAYY285 pKa = 7.43LTLRR289 pKa = 11.84VLRR292 pKa = 11.84NALDD296 pKa = 3.9GVDD299 pKa = 3.46IDD301 pKa = 4.49TGIGTPDD308 pKa = 3.04EE309 pKa = 4.64AGNVLSSDD317 pKa = 3.51VFTYY321 pKa = 10.81NADD324 pKa = 3.32EE325 pKa = 4.15RR326 pKa = 11.84SYY328 pKa = 11.15YY329 pKa = 10.26ALNVAVTADD338 pKa = 3.58NYY340 pKa = 11.01QDD342 pKa = 4.45FLDD345 pKa = 4.02STVVYY350 pKa = 10.65APVSNQLDD358 pKa = 3.96AEE360 pKa = 4.2AHH362 pKa = 5.04PTKK365 pKa = 10.73NVWLNIYY372 pKa = 9.75NAADD376 pKa = 3.49NFLSATYY383 pKa = 10.31QPLLQNYY390 pKa = 9.65DD391 pKa = 3.59DD392 pKa = 4.97LLNLNVEE399 pKa = 4.63YY400 pKa = 10.44IGGDD404 pKa = 3.26GQTEE408 pKa = 4.46SNITNRR414 pKa = 11.84LGNPSQYY421 pKa = 11.16DD422 pKa = 3.19AFAINMVKK430 pKa = 9.6TDD432 pKa = 3.32NATSYY437 pKa = 7.53TTLLSQQ443 pKa = 4.58

Molecular weight: 46.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Y4IPP2|A0A1Y4IPP2_9FIRM ABC transporter ATP-binding protein OS=Lachnoclostridium sp. An196 OX=1965583 GN=B5F29_02840 PE=4 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 9.06KK9 pKa = 8.03RR10 pKa = 11.84SRR12 pKa = 11.84SKK14 pKa = 9.44VHH16 pKa = 6.17GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTKK25 pKa = 10.15GGRR28 pKa = 11.84KK29 pKa = 8.69VLAARR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 10.61GRR39 pKa = 11.84KK40 pKa = 8.87KK41 pKa = 10.59LSAA44 pKa = 3.95

Molecular weight: 5.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3081

0

3081

977824

25

5186

317.4

35.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.996 ± 0.041

1.565 ± 0.021

5.738 ± 0.037

8.2 ± 0.048

3.885 ± 0.03

7.417 ± 0.043

1.742 ± 0.021

6.54 ± 0.037

5.708 ± 0.048

9.346 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.077 ± 0.029

3.661 ± 0.031

3.531 ± 0.023

3.402 ± 0.028

5.457 ± 0.046

5.565 ± 0.041

5.205 ± 0.051

6.848 ± 0.039

1.036 ± 0.016

4.08 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski