Amino acid dipepetide frequency for Lachnoclostridium sp. An196

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.896AlaAla: 8.896 ± 0.14
1.377AlaCys: 1.377 ± 0.044
5.096AlaAsp: 5.096 ± 0.081
6.648AlaGlu: 6.648 ± 0.105
3.107AlaPhe: 3.107 ± 0.067
7.18AlaGly: 7.18 ± 0.094
1.171AlaHis: 1.171 ± 0.039
4.098AlaIle: 4.098 ± 0.071
4.062AlaLys: 4.062 ± 0.075
7.555AlaLeu: 7.555 ± 0.113
2.37AlaMet: 2.37 ± 0.058
2.236AlaAsn: 2.236 ± 0.06
2.206AlaPro: 2.206 ± 0.058
2.456AlaGln: 2.456 ± 0.057
3.816AlaArg: 3.816 ± 0.069
4.176AlaSer: 4.176 ± 0.069
2.74AlaThr: 2.74 ± 0.062
7.122AlaVal: 7.122 ± 0.097
0.784AlaTrp: 0.784 ± 0.03
2.861AlaTyr: 2.861 ± 0.059
0.0AlaXaa: 0.0 ± 0.0
Cys
1.218CysAla: 1.218 ± 0.036
0.311CysCys: 0.311 ± 0.021
0.739CysAsp: 0.739 ± 0.032
0.894CysGlu: 0.894 ± 0.036
0.605CysPhe: 0.605 ± 0.027
1.685CysGly: 1.685 ± 0.053
0.325CysHis: 0.325 ± 0.019
1.081CysIle: 1.081 ± 0.036
0.68CysLys: 0.68 ± 0.028
1.418CysLeu: 1.418 ± 0.037
0.528CysMet: 0.528 ± 0.024
0.443CysAsn: 0.443 ± 0.022
0.733CysPro: 0.733 ± 0.029
0.395CysGln: 0.395 ± 0.019
1.127CysArg: 1.127 ± 0.038
0.911CysSer: 0.911 ± 0.031
0.735CysThr: 0.735 ± 0.03
1.117CysVal: 1.117 ± 0.037
0.156CysTrp: 0.156 ± 0.012
0.548CysTyr: 0.548 ± 0.024
0.0CysXaa: 0.0 ± 0.0
Asp
4.635AspAla: 4.635 ± 0.082
0.829AspCys: 0.829 ± 0.032
2.84AspAsp: 2.84 ± 0.059
4.753AspGlu: 4.753 ± 0.08
2.501AspPhe: 2.501 ± 0.059
4.861AspGly: 4.861 ± 0.105
0.985AspHis: 0.985 ± 0.039
3.858AspIle: 3.858 ± 0.065
2.611AspLys: 2.611 ± 0.053
5.224AspLeu: 5.224 ± 0.078
1.863AspMet: 1.863 ± 0.041
1.795AspAsn: 1.795 ± 0.051
2.203AspPro: 2.203 ± 0.057
1.857AspGln: 1.857 ± 0.039
3.208AspArg: 3.208 ± 0.065
3.027AspSer: 3.027 ± 0.065
3.155AspThr: 3.155 ± 0.069
3.762AspVal: 3.762 ± 0.067
0.642AspTrp: 0.642 ± 0.029
2.771AspTyr: 2.771 ± 0.06
0.0AspXaa: 0.0 ± 0.0
Glu
6.441GluAla: 6.441 ± 0.099
0.88GluCys: 0.88 ± 0.03
4.804GluAsp: 4.804 ± 0.085
8.774GluGlu: 8.774 ± 0.138
2.508GluPhe: 2.508 ± 0.055
5.075GluGly: 5.075 ± 0.089
1.507GluHis: 1.507 ± 0.044
5.575GluIle: 5.575 ± 0.085
6.246GluLys: 6.246 ± 0.1
7.34GluLeu: 7.34 ± 0.096
2.469GluMet: 2.469 ± 0.048
3.842GluAsn: 3.842 ± 0.069
2.24GluPro: 2.24 ± 0.054
3.429GluGln: 3.429 ± 0.07
4.309GluArg: 4.309 ± 0.085
3.557GluSer: 3.557 ± 0.064
4.215GluThr: 4.215 ± 0.093
4.547GluVal: 4.547 ± 0.071
0.782GluTrp: 0.782 ± 0.03
3.462GluTyr: 3.462 ± 0.065
0.0GluXaa: 0.0 ± 0.0
Phe
2.882PheAla: 2.882 ± 0.055
0.81PheCys: 0.81 ± 0.033
2.357PheAsp: 2.357 ± 0.048
2.427PheGlu: 2.427 ± 0.052
1.666PhePhe: 1.666 ± 0.047
2.978PheGly: 2.978 ± 0.06
0.795PheHis: 0.795 ± 0.031
2.227PheIle: 2.227 ± 0.062
1.345PheLys: 1.345 ± 0.041
4.29PheLeu: 4.29 ± 0.082
1.027PheMet: 1.027 ± 0.035
1.234PheAsn: 1.234 ± 0.037
1.509PhePro: 1.509 ± 0.039
1.428PheGln: 1.428 ± 0.037
2.307PheArg: 2.307 ± 0.053
2.746PheSer: 2.746 ± 0.053
2.161PheThr: 2.161 ± 0.057
2.532PheVal: 2.532 ± 0.053
0.513PheTrp: 0.513 ± 0.028
1.615PheTyr: 1.615 ± 0.045
0.0PheXaa: 0.0 ± 0.0
Gly
5.57GlyAla: 5.57 ± 0.091
1.335GlyCys: 1.335 ± 0.044
3.809GlyAsp: 3.809 ± 0.064
5.306GlyGlu: 5.306 ± 0.085
2.947GlyPhe: 2.947 ± 0.057
5.059GlyGly: 5.059 ± 0.086
1.27GlyHis: 1.27 ± 0.035
6.166GlyIle: 6.166 ± 0.083
5.199GlyLys: 5.199 ± 0.079
6.123GlyLeu: 6.123 ± 0.088
2.588GlyMet: 2.588 ± 0.055
2.897GlyAsn: 2.897 ± 0.062
1.432GlyPro: 1.432 ± 0.045
2.375GlyGln: 2.375 ± 0.05
3.895GlyArg: 3.895 ± 0.069
4.436GlySer: 4.436 ± 0.085
4.552GlyThr: 4.552 ± 0.091
5.054GlyVal: 5.054 ± 0.084
0.852GlyTrp: 0.852 ± 0.03
3.307GlyTyr: 3.307 ± 0.068
0.0GlyXaa: 0.0 ± 0.0
His
1.242HisAla: 1.242 ± 0.036
0.313HisCys: 0.313 ± 0.016
0.896HisAsp: 0.896 ± 0.035
1.137HisGlu: 1.137 ± 0.036
0.837HisPhe: 0.837 ± 0.034
1.318HisGly: 1.318 ± 0.038
0.449HisHis: 0.449 ± 0.024
1.25HisIle: 1.25 ± 0.032
0.748HisLys: 0.748 ± 0.029
1.613HisLeu: 1.613 ± 0.047
0.613HisMet: 0.613 ± 0.023
0.621HisAsn: 0.621 ± 0.023
0.973HisPro: 0.973 ± 0.033
0.556HisGln: 0.556 ± 0.024
0.929HisArg: 0.929 ± 0.036
0.836HisSer: 0.836 ± 0.026
1.016HisThr: 1.016 ± 0.034
1.159HisVal: 1.159 ± 0.034
0.169HisTrp: 0.169 ± 0.013
0.753HisTyr: 0.753 ± 0.03
0.0HisXaa: 0.0 ± 0.0
Ile
5.037IleAla: 5.037 ± 0.093
1.323IleCys: 1.323 ± 0.04
3.549IleAsp: 3.549 ± 0.066
4.113IleGlu: 4.113 ± 0.072
2.592IlePhe: 2.592 ± 0.055
4.636IleGly: 4.636 ± 0.081
1.314IleHis: 1.314 ± 0.038
3.792IleIle: 3.792 ± 0.083
2.781IleLys: 2.781 ± 0.053
7.085IleLeu: 7.085 ± 0.094
1.751IleMet: 1.751 ± 0.043
2.066IleAsn: 2.066 ± 0.047
3.162IlePro: 3.162 ± 0.06
2.42IleGln: 2.42 ± 0.051
4.704IleArg: 4.704 ± 0.082
4.209IleSer: 4.209 ± 0.077
3.342IleThr: 3.342 ± 0.069
4.252IleVal: 4.252 ± 0.086
0.687IleTrp: 0.687 ± 0.028
2.582IleTyr: 2.582 ± 0.06
0.0IleXaa: 0.0 ± 0.0
Lys
4.565LysAla: 4.565 ± 0.079
0.563LysCys: 0.563 ± 0.027
3.406LysAsp: 3.406 ± 0.065
5.774LysGlu: 5.774 ± 0.083
1.405LysPhe: 1.405 ± 0.042
3.701LysGly: 3.701 ± 0.068
0.862LysHis: 0.862 ± 0.03
3.899LysIle: 3.899 ± 0.061
4.896LysLys: 4.896 ± 0.081
4.57LysLeu: 4.57 ± 0.065
1.839LysMet: 1.839 ± 0.049
2.694LysAsn: 2.694 ± 0.055
1.758LysPro: 1.758 ± 0.043
1.813LysGln: 1.813 ± 0.05
2.932LysArg: 2.932 ± 0.055
2.72LysSer: 2.72 ± 0.062
3.166LysThr: 3.166 ± 0.058
3.562LysVal: 3.562 ± 0.068
0.537LysTrp: 0.537 ± 0.029
2.421LysTyr: 2.421 ± 0.055
0.0LysXaa: 0.0 ± 0.0
Leu
7.565LeuAla: 7.565 ± 0.109
1.625LeuCys: 1.625 ± 0.048
5.523LeuAsp: 5.523 ± 0.073
7.518LeuGlu: 7.518 ± 0.097
3.724LeuPhe: 3.724 ± 0.077
6.205LeuGly: 6.205 ± 0.098
1.605LeuHis: 1.605 ± 0.044
5.703LeuIle: 5.703 ± 0.085
5.599LeuLys: 5.599 ± 0.081
9.368LeuLeu: 9.368 ± 0.155
2.689LeuMet: 2.689 ± 0.055
3.426LeuAsn: 3.426 ± 0.059
3.851LeuPro: 3.851 ± 0.064
3.059LeuGln: 3.059 ± 0.057
4.686LeuArg: 4.686 ± 0.085
6.163LeuSer: 6.163 ± 0.088
5.212LeuThr: 5.212 ± 0.078
5.578LeuVal: 5.578 ± 0.098
0.864LeuTrp: 0.864 ± 0.034
3.504LeuTyr: 3.504 ± 0.074
0.0LeuXaa: 0.0 ± 0.0
Met
2.54MetAla: 2.54 ± 0.063
0.312MetCys: 0.312 ± 0.019
2.034MetAsp: 2.034 ± 0.044
2.895MetGlu: 2.895 ± 0.062
0.973MetPhe: 0.973 ± 0.033
2.191MetGly: 2.191 ± 0.054
0.467MetHis: 0.467 ± 0.021
1.999MetIle: 1.999 ± 0.049
2.362MetLys: 2.362 ± 0.045
2.68MetLeu: 2.68 ± 0.063
0.886MetMet: 0.886 ± 0.033
1.402MetAsn: 1.402 ± 0.041
1.185MetPro: 1.185 ± 0.029
1.003MetGln: 1.003 ± 0.035
1.443MetArg: 1.443 ± 0.04
1.681MetSer: 1.681 ± 0.041
1.785MetThr: 1.785 ± 0.039
1.869MetVal: 1.869 ± 0.049
0.235MetTrp: 0.235 ± 0.017
0.821MetTyr: 0.821 ± 0.028
0.0MetXaa: 0.0 ± 0.0
Asn
2.771AsnAla: 2.771 ± 0.057
0.582AsnCys: 0.582 ± 0.027
1.708AsnAsp: 1.708 ± 0.04
2.403AsnGlu: 2.403 ± 0.056
1.324AsnPhe: 1.324 ± 0.039
3.146AsnGly: 3.146 ± 0.072
0.715AsnHis: 0.715 ± 0.03
2.631AsnIle: 2.631 ± 0.053
1.647AsnLys: 1.647 ± 0.049
3.469AsnLeu: 3.469 ± 0.063
1.214AsnMet: 1.214 ± 0.037
1.313AsnAsn: 1.313 ± 0.042
1.814AsnPro: 1.814 ± 0.042
1.327AsnGln: 1.327 ± 0.036
2.248AsnArg: 2.248 ± 0.052
1.82AsnSer: 1.82 ± 0.046
1.973AsnThr: 1.973 ± 0.057
2.517AsnVal: 2.517 ± 0.06
0.397AsnTrp: 0.397 ± 0.023
1.592AsnTyr: 1.592 ± 0.041
0.0AsnXaa: 0.0 ± 0.0
Pro
2.948ProAla: 2.948 ± 0.059
0.496ProCys: 0.496 ± 0.022
2.792ProAsp: 2.792 ± 0.062
4.136ProGlu: 4.136 ± 0.085
1.614ProPhe: 1.614 ± 0.046
2.725ProGly: 2.725 ± 0.057
0.612ProHis: 0.612 ± 0.029
1.694ProIle: 1.694 ± 0.046
1.609ProLys: 1.609 ± 0.042
2.922ProLeu: 2.922 ± 0.055
0.899ProMet: 0.899 ± 0.033
1.064ProAsn: 1.064 ± 0.04
0.907ProPro: 0.907 ± 0.028
1.065ProGln: 1.065 ± 0.035
1.34ProArg: 1.34 ± 0.033
1.862ProSer: 1.862 ± 0.045
1.452ProThr: 1.452 ± 0.05
3.363ProVal: 3.363 ± 0.067
0.357ProTrp: 0.357 ± 0.022
1.452ProTyr: 1.452 ± 0.036
0.0ProXaa: 0.0 ± 0.0
Gln
2.8GlnAla: 2.8 ± 0.062
0.398GlnCys: 0.398 ± 0.023
1.794GlnAsp: 1.794 ± 0.051
3.306GlnGlu: 3.306 ± 0.063
1.123GlnPhe: 1.123 ± 0.035
2.135GlnGly: 2.135 ± 0.047
0.423GlnHis: 0.423 ± 0.023
2.722GlnIle: 2.722 ± 0.048
2.501GlnLys: 2.501 ± 0.057
2.68GlnLeu: 2.68 ± 0.057
1.255GlnMet: 1.255 ± 0.037
1.589GlnAsn: 1.589 ± 0.044
0.982GlnPro: 0.982 ± 0.036
1.196GlnGln: 1.196 ± 0.039
1.59GlnArg: 1.59 ± 0.051
1.577GlnSer: 1.577 ± 0.041
1.848GlnThr: 1.848 ± 0.047
2.343GlnVal: 2.343 ± 0.051
0.336GlnTrp: 0.336 ± 0.017
1.418GlnTyr: 1.418 ± 0.041
0.0GlnXaa: 0.0 ± 0.0
Arg
3.564ArgAla: 3.564 ± 0.072
0.761ArgCys: 0.761 ± 0.03
2.69ArgAsp: 2.69 ± 0.056
5.022ArgGlu: 5.022 ± 0.087
2.214ArgPhe: 2.214 ± 0.048
3.054ArgGly: 3.054 ± 0.068
0.947ArgHis: 0.947 ± 0.031
4.24ArgIle: 4.24 ± 0.068
3.896ArgLys: 3.896 ± 0.068
4.981ArgLeu: 4.981 ± 0.082
2.025ArgMet: 2.025 ± 0.05
2.123ArgAsn: 2.123 ± 0.048
1.784ArgPro: 1.784 ± 0.047
2.203ArgGln: 2.203 ± 0.055
3.542ArgArg: 3.542 ± 0.077
2.682ArgSer: 2.682 ± 0.057
2.839ArgThr: 2.839 ± 0.055
3.135ArgVal: 3.135 ± 0.067
0.549ArgTrp: 0.549 ± 0.025
2.323ArgTyr: 2.323 ± 0.051
0.0ArgXaa: 0.0 ± 0.0
Ser
4.491SerAla: 4.491 ± 0.076
0.889SerCys: 0.889 ± 0.029
3.09SerAsp: 3.09 ± 0.077
3.905SerGlu: 3.905 ± 0.076
2.455SerPhe: 2.455 ± 0.052
5.341SerGly: 5.341 ± 0.091
0.996SerHis: 0.996 ± 0.037
3.328SerIle: 3.328 ± 0.063
2.305SerLys: 2.305 ± 0.056
5.19SerLeu: 5.19 ± 0.079
1.817SerMet: 1.817 ± 0.044
1.688SerAsn: 1.688 ± 0.049
1.895SerPro: 1.895 ± 0.044
1.785SerGln: 1.785 ± 0.038
3.465SerArg: 3.465 ± 0.067
3.332SerSer: 3.332 ± 0.074
2.457SerThr: 2.457 ± 0.061
4.236SerVal: 4.236 ± 0.061
0.673SerTrp: 0.673 ± 0.032
2.315SerTyr: 2.315 ± 0.052
0.0SerXaa: 0.0 ± 0.0
Thr
4.729ThrAla: 4.729 ± 0.086
0.655ThrCys: 0.655 ± 0.026
3.42ThrAsp: 3.42 ± 0.078
4.145ThrGlu: 4.145 ± 0.082
1.994ThrPhe: 1.994 ± 0.049
4.995ThrGly: 4.995 ± 0.078
0.801ThrHis: 0.801 ± 0.03
3.166ThrIle: 3.166 ± 0.066
2.258ThrLys: 2.258 ± 0.053
4.763ThrLeu: 4.763 ± 0.076
1.393ThrMet: 1.393 ± 0.037
1.591ThrAsn: 1.591 ± 0.046
2.163ThrPro: 2.163 ± 0.049
1.406ThrGln: 1.406 ± 0.042
2.228ThrArg: 2.228 ± 0.054
2.621ThrSer: 2.621 ± 0.061
2.462ThrThr: 2.462 ± 0.066
4.771ThrVal: 4.771 ± 0.112
0.526ThrTrp: 0.526 ± 0.024
1.959ThrTyr: 1.959 ± 0.062
0.0ThrXaa: 0.0 ± 0.0
Val
4.525ValAla: 4.525 ± 0.075
1.377ValCys: 1.377 ± 0.042
3.711ValAsp: 3.711 ± 0.064
4.774ValGlu: 4.774 ± 0.074
2.982ValPhe: 2.982 ± 0.064
4.119ValGly: 4.119 ± 0.08
1.138ValHis: 1.138 ± 0.039
4.717ValIle: 4.717 ± 0.069
3.83ValLys: 3.83 ± 0.07
7.085ValLeu: 7.085 ± 0.109
2.044ValMet: 2.044 ± 0.05
2.576ValAsn: 2.576 ± 0.049
2.772ValPro: 2.772 ± 0.05
2.245ValGln: 2.245 ± 0.059
3.827ValArg: 3.827 ± 0.06
4.649ValSer: 4.649 ± 0.072
4.175ValThr: 4.175 ± 0.091
4.429ValVal: 4.429 ± 0.093
0.764ValTrp: 0.764 ± 0.031
2.744ValTyr: 2.744 ± 0.061
0.0ValXaa: 0.0 ± 0.0
Trp
0.621TrpAla: 0.621 ± 0.026
0.147TrpCys: 0.147 ± 0.014
0.619TrpAsp: 0.619 ± 0.024
0.824TrpGlu: 0.824 ± 0.031
0.479TrpPhe: 0.479 ± 0.024
0.673TrpGly: 0.673 ± 0.03
0.163TrpHis: 0.163 ± 0.012
0.727TrpIle: 0.727 ± 0.029
0.828TrpLys: 0.828 ± 0.032
1.058TrpLeu: 1.058 ± 0.033
0.384TrpMet: 0.384 ± 0.019
0.574TrpAsn: 0.574 ± 0.025
0.243TrpPro: 0.243 ± 0.015
0.418TrpGln: 0.418 ± 0.021
0.493TrpArg: 0.493 ± 0.024
0.512TrpSer: 0.512 ± 0.026
0.514TrpThr: 0.514 ± 0.029
0.531TrpVal: 0.531 ± 0.025
0.099TrpTrp: 0.099 ± 0.01
0.453TrpTyr: 0.453 ± 0.021
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.917TyrAla: 2.917 ± 0.061
0.67TyrCys: 0.67 ± 0.029
2.506TyrAsp: 2.506 ± 0.058
3.244TyrGlu: 3.244 ± 0.07
1.799TyrPhe: 1.799 ± 0.043
3.096TyrGly: 3.096 ± 0.066
0.878TyrHis: 0.878 ± 0.031
2.525TyrIle: 2.525 ± 0.049
1.681TyrLys: 1.681 ± 0.046
4.05TyrLeu: 4.05 ± 0.073
1.113TyrMet: 1.113 ± 0.034
1.438TyrAsn: 1.438 ± 0.042
1.494TyrPro: 1.494 ± 0.035
1.583TyrGln: 1.583 ± 0.036
2.485TyrArg: 2.485 ± 0.054
2.134TyrSer: 2.134 ± 0.056
2.247TyrThr: 2.247 ± 0.066
2.607TyrVal: 2.607 ± 0.06
0.437TyrTrp: 0.437 ± 0.022
1.898TyrTyr: 1.898 ± 0.057
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3081 proteins (977824 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski