Hibiscus latent Fort Pierce virus
Average proteome isoelectric point is 6.04
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A097ZNB1|A0A097ZNB1_9VIRU 31 kDa movement protein OS=Hibiscus latent Fort Pierce virus OX=233051 PE=4 SV=1
MM1 pKa = 7.57 SYY3 pKa = 11.7 SNITALNLIYY13 pKa = 10.45 SSPTFAPYY21 pKa = 10.45 DD22 pKa = 3.59 VLLEE26 pKa = 3.86 ILIKK30 pKa = 10.67 SRR32 pKa = 11.84 SNSFQTQAGRR42 pKa = 11.84 DD43 pKa = 3.44 ILRR46 pKa = 11.84 EE47 pKa = 3.79 QLVNALQIVVTPTTRR62 pKa = 11.84 FPADD66 pKa = 2.77 RR67 pKa = 11.84 WYY69 pKa = 10.26 VWSRR73 pKa = 11.84 NPTLGPVFEE82 pKa = 5.67 ALLQATDD89 pKa = 3.35 TKK91 pKa = 11.33 NRR93 pKa = 11.84 IIEE96 pKa = 4.26 TEE98 pKa = 3.84 EE99 pKa = 3.89 DD100 pKa = 3.67 SRR102 pKa = 11.84 PTTAEE107 pKa = 3.93 TLNATQRR114 pKa = 11.84 VDD116 pKa = 3.35 DD117 pKa = 4.06 ATVAIHH123 pKa = 7.12 KK124 pKa = 9.93 EE125 pKa = 3.3 IDD127 pKa = 3.73 NILGLLQGGTAVYY140 pKa = 9.81 NQASFEE146 pKa = 4.19 IASGWTWTAPANN158 pKa = 3.7
Molecular weight: 17.6 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.823
IPC2_protein 4.851
IPC_protein 4.698
Toseland 4.558
ProMoST 4.813
Dawson 4.647
Bjellqvist 4.8
Wikipedia 4.52
Rodwell 4.546
Grimsley 4.469
Solomon 4.647
Lehninger 4.596
Nozaki 4.762
DTASelect 4.914
Thurlkill 4.558
EMBOSS 4.533
Sillero 4.813
Patrickios 4.101
IPC_peptide 4.647
IPC2_peptide 4.813
IPC2.peptide.svr19 4.788
Protein with the highest isoelectric point:
>tr|B6E277|B6E277_9VIRU 128 kDa replicase OS=Hibiscus latent Fort Pierce virus OX=233051 PE=4 SV=1
MM1 pKa = 6.62 TTSTTDD7 pKa = 2.67 IKK9 pKa = 10.91 QQITNVRR16 pKa = 11.84 DD17 pKa = 3.73 DD18 pKa = 4.12 AAHH21 pKa = 6.6 GAASLIKK28 pKa = 10.28 GLATRR33 pKa = 11.84 RR34 pKa = 11.84 VYY36 pKa = 11.08 DD37 pKa = 3.58 EE38 pKa = 4.64 AVNTLTLLDD47 pKa = 4.25 KK48 pKa = 10.73 RR49 pKa = 11.84 PKK51 pKa = 10.21 FSFSNLVSTEE61 pKa = 3.67 NAKK64 pKa = 9.42 MVTEE68 pKa = 5.5 AYY70 pKa = 9.73 PEE72 pKa = 4.04 FNISFVGNRR81 pKa = 11.84 NTVHH85 pKa = 6.58 SLAGGLRR92 pKa = 11.84 KK93 pKa = 9.93 LEE95 pKa = 4.02 MEE97 pKa = 4.48 YY98 pKa = 11.29 LMTLVPYY105 pKa = 10.68 GSATFDD111 pKa = 2.71 IGGNYY116 pKa = 8.98 AQHH119 pKa = 6.51 LLKK122 pKa = 10.75 GRR124 pKa = 11.84 AYY126 pKa = 8.8 VHH128 pKa = 6.65 CCNPCLDD135 pKa = 3.98 FRR137 pKa = 11.84 DD138 pKa = 3.57 IARR141 pKa = 11.84 NEE143 pKa = 4.27 SYY145 pKa = 11.08 KK146 pKa = 10.53 DD147 pKa = 3.53 TVDD150 pKa = 3.8 SYY152 pKa = 11.66 LKK154 pKa = 10.69 RR155 pKa = 11.84 FTTPDD160 pKa = 3.61 CGWFSGAVRR169 pKa = 11.84 AITARR174 pKa = 11.84 PLPSFQKK181 pKa = 10.5 EE182 pKa = 3.52 AFEE185 pKa = 5.24 RR186 pKa = 11.84 YY187 pKa = 8.8 RR188 pKa = 11.84 QVPSDD193 pKa = 3.86 VTCSQPFQDD202 pKa = 4.24 CAMRR206 pKa = 11.84 TPPNQDD212 pKa = 2.69 CFAISVHH219 pKa = 5.68 SLYY222 pKa = 10.65 DD223 pKa = 3.34 IPVDD227 pKa = 3.75 EE228 pKa = 5.53 LGCALLRR235 pKa = 11.84 KK236 pKa = 9.07 NVKK239 pKa = 9.15 ILYY242 pKa = 9.05 AALHH246 pKa = 5.77 FSEE249 pKa = 5.72 DD250 pKa = 3.8 LLLGATDD257 pKa = 4.19 APLGNIGARR266 pKa = 11.84 FYY268 pKa = 11.36 RR269 pKa = 11.84 NGDD272 pKa = 3.24 EE273 pKa = 4.23 VTFGFDD279 pKa = 3.67 GEE281 pKa = 4.6 STLLYY286 pKa = 9.15 THH288 pKa = 6.46 SFKK291 pKa = 11.15 NICGYY296 pKa = 7.53 ITRR299 pKa = 11.84 SFFYY303 pKa = 10.6 AGAKK307 pKa = 7.48 YY308 pKa = 10.44 AYY310 pKa = 9.09 MKK312 pKa = 10.55 EE313 pKa = 4.12 FMVKK317 pKa = 10.01 RR318 pKa = 11.84 VDD320 pKa = 3.44 TVFCKK325 pKa = 10.42 FVRR328 pKa = 11.84 IDD330 pKa = 3.49 TQCLYY335 pKa = 11.03 KK336 pKa = 10.61 SVFHH340 pKa = 5.82 TQIDD344 pKa = 3.6 SDD346 pKa = 4.19 AVLEE350 pKa = 4.53 GMDD353 pKa = 3.48 SAFTARR359 pKa = 11.84 RR360 pKa = 11.84 QAAMLNAARR369 pKa = 11.84 PLFKK373 pKa = 10.72 DD374 pKa = 3.17 KK375 pKa = 11.17 AAFSVWFPNAIGKK388 pKa = 8.43 VLIPIFRR395 pKa = 11.84 GTTCLGAKK403 pKa = 9.55 IKK405 pKa = 9.64 MEE407 pKa = 3.89 KK408 pKa = 10.27 RR409 pKa = 11.84 LVDD412 pKa = 3.45 EE413 pKa = 4.89 DD414 pKa = 3.97 FVYY417 pKa = 10.39 TILNHH422 pKa = 5.29 IRR424 pKa = 11.84 TYY426 pKa = 9.69 TGKK429 pKa = 9.52 QLSYY433 pKa = 10.83 EE434 pKa = 4.14 NVLSFVEE441 pKa = 4.76 SIRR444 pKa = 11.84 SRR446 pKa = 11.84 VVINGANVRR455 pKa = 11.84 SEE457 pKa = 3.84 WNIPKK462 pKa = 10.14 SDD464 pKa = 3.44 IQDD467 pKa = 2.98 IAMSLFLITKK477 pKa = 9.88 LRR479 pKa = 11.84 TLQDD483 pKa = 3.56 SAVLSHH489 pKa = 7.2 FDD491 pKa = 3.13 IKK493 pKa = 11.08 KK494 pKa = 10.37 KK495 pKa = 10.94 GFFDD499 pKa = 4.11 CVRR502 pKa = 11.84 EE503 pKa = 4.18 TIMEE507 pKa = 4.13 VTGSIFEE514 pKa = 4.82 PLTQICIDD522 pKa = 3.94 HH523 pKa = 6.46 GWFKK527 pKa = 10.83 IKK529 pKa = 10.06 EE530 pKa = 4.19 DD531 pKa = 3.52 KK532 pKa = 11.21 LKK534 pKa = 11.3 VEE536 pKa = 4.51 VPDD539 pKa = 3.56 TSRR542 pKa = 11.84 TFMSYY547 pKa = 8.14 MQTEE551 pKa = 4.64 FSEE554 pKa = 4.67 SKK556 pKa = 10.63 AIEE559 pKa = 4.32 SLDD562 pKa = 3.07 LWEE565 pKa = 5.68 YY566 pKa = 11.16 LDD568 pKa = 5.44 QSNQLYY574 pKa = 7.8 THH576 pKa = 6.26 VSKK579 pKa = 11.03 LMEE582 pKa = 5.03 RR583 pKa = 11.84 YY584 pKa = 9.49 SLPDD588 pKa = 3.42 FDD590 pKa = 4.71 VEE592 pKa = 4.97 KK593 pKa = 11.03 FKK595 pKa = 11.05 DD596 pKa = 3.46 LCAHH600 pKa = 6.62 LKK602 pKa = 10.17 VGPEE606 pKa = 4.0 VITAVIEE613 pKa = 4.07 AVMDD617 pKa = 3.62 KK618 pKa = 11.02 SLGFTVVGGKK628 pKa = 9.86 AEE630 pKa = 4.1 HH631 pKa = 6.32 QFTDD635 pKa = 3.13 EE636 pKa = 3.98 MKK638 pKa = 10.68 AAVAVSADD646 pKa = 3.86 YY647 pKa = 10.57 GQHH650 pKa = 5.59 CTDD653 pKa = 3.49 KK654 pKa = 11.01 KK655 pKa = 10.33 QVASAACKK663 pKa = 10.76 NEE665 pKa = 3.67 VTKK668 pKa = 10.18 PLKK671 pKa = 9.97 KK672 pKa = 9.95 QWVEE676 pKa = 3.68 KK677 pKa = 7.99 TTAALPLQATTEE689 pKa = 4.3 KK690 pKa = 9.46 TQHH693 pKa = 5.08 FHH695 pKa = 7.43 FNDD698 pKa = 3.4 EE699 pKa = 4.51 DD700 pKa = 3.92 EE701 pKa = 5.12 SISVEE706 pKa = 3.96 NLHH709 pKa = 6.87 LKK711 pKa = 10.39 RR712 pKa = 11.84 ADD714 pKa = 3.29 TFKK717 pKa = 11.05 ASKK720 pKa = 10.92 ACMLYY725 pKa = 9.49 TGTVRR730 pKa = 11.84 EE731 pKa = 4.01 QQMKK735 pKa = 10.79 NFLDD739 pKa = 3.7 YY740 pKa = 11.22 LSASICATISNIEE753 pKa = 4.28 KK754 pKa = 10.37 VLSDD758 pKa = 3.27 YY759 pKa = 9.97 WVSGTQTYY767 pKa = 7.2 QTYY770 pKa = 10.94 GLWDD774 pKa = 3.78 CKK776 pKa = 10.14 KK777 pKa = 10.28 KK778 pKa = 10.49 KK779 pKa = 9.12 WVLQPPTAGHH789 pKa = 5.64 SWGIASIKK797 pKa = 10.26 GEE799 pKa = 4.24 HH800 pKa = 6.76 FIVMLSFNEE809 pKa = 4.14 NVEE812 pKa = 4.65 PICDD816 pKa = 3.87 SSWDD820 pKa = 3.9 MVCVSNDD827 pKa = 3.19 TKK829 pKa = 11.02 LFSAMKK835 pKa = 9.65 ILEE838 pKa = 4.52 NLTPLPVKK846 pKa = 10.22 EE847 pKa = 4.43 SNAKK851 pKa = 7.91 ITLVDD856 pKa = 4.08 GVPGCGKK863 pKa = 8.25 TAEE866 pKa = 4.39 ILAKK870 pKa = 10.84 ANFKK874 pKa = 10.59 KK875 pKa = 10.67 DD876 pKa = 3.98 LILTQGKK883 pKa = 8.53 QAAQMIRR890 pKa = 11.84 KK891 pKa = 8.52 RR892 pKa = 11.84 ANAIDD897 pKa = 3.78 CTKK900 pKa = 10.2 PANTSNVRR908 pKa = 11.84 TVDD911 pKa = 3.54 SFLMNPKK918 pKa = 9.83 PFTYY922 pKa = 8.69 DD923 pKa = 3.26 TLWIDD928 pKa = 3.74 EE929 pKa = 4.32 GLMLHH934 pKa = 6.37 TGMVNYY940 pKa = 10.12 CVLLSHH946 pKa = 6.35 CTKK949 pKa = 10.59 CFIYY953 pKa = 10.7 GDD955 pKa = 3.79 TQQIPFINRR964 pKa = 11.84 VMNFDD969 pKa = 3.77 YY970 pKa = 10.49 PGHH973 pKa = 6.82 LRR975 pKa = 11.84 TIVVDD980 pKa = 3.55 DD981 pKa = 3.81 TEE983 pKa = 4.24 KK984 pKa = 10.82 RR985 pKa = 11.84 RR986 pKa = 11.84 ITSRR990 pKa = 11.84 CPLDD994 pKa = 3.19 VTFYY998 pKa = 10.03 LTQRR1002 pKa = 11.84 YY1003 pKa = 8.2 KK1004 pKa = 11.04 GPVMSTSSVTRR1015 pKa = 11.84 SVEE1018 pKa = 4.18 TKK1020 pKa = 10.34 FVAGPARR1027 pKa = 11.84 FEE1029 pKa = 4.15 PRR1031 pKa = 11.84 ATPLPGKK1038 pKa = 10.06 IVTFTQADD1046 pKa = 3.86 KK1047 pKa = 10.14 EE1048 pKa = 4.58 TLKK1051 pKa = 11.04 KK1052 pKa = 10.25 KK1053 pKa = 10.45 GYY1055 pKa = 9.04 NDD1057 pKa = 2.88 VHH1059 pKa = 5.93 TVHH1062 pKa = 7.38 EE1063 pKa = 4.44 IQGEE1067 pKa = 4.47 TFNEE1071 pKa = 4.09 VSLVRR1076 pKa = 11.84 LTATPLNIISPEE1088 pKa = 4.11 SPHH1091 pKa = 7.52 VLVGLSRR1098 pKa = 11.84 HH1099 pKa = 5.26 TNRR1102 pKa = 11.84 LTYY1105 pKa = 8.44 YY1106 pKa = 9.69 TVVHH1110 pKa = 6.72 DD1111 pKa = 5.38 CITYY1115 pKa = 10.33 SISEE1119 pKa = 4.34 LSRR1122 pKa = 11.84 LSDD1125 pKa = 4.15 YY1126 pKa = 11.22 IFDD1129 pKa = 3.84 IYY1131 pKa = 11.17 SVEE1134 pKa = 4.08 GQKK1137 pKa = 10.71 AA1138 pKa = 2.99
Molecular weight: 128.48 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.749
IPC2_protein 7.044
IPC_protein 6.898
Toseland 6.722
ProMoST 7.497
Dawson 7.556
Bjellqvist 7.936
Wikipedia 7.468
Rodwell 7.571
Grimsley 6.81
Solomon 7.571
Lehninger 7.585
Nozaki 8.2
DTASelect 7.761
Thurlkill 7.805
EMBOSS 7.805
Sillero 8.097
Patrickios 4.164
IPC_peptide 7.571
IPC2_peptide 7.176
IPC2.peptide.svr19 7.073
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
3226
158
1646
806.5
90.92
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.789 ± 0.412
2.356 ± 0.348
6.603 ± 0.267
5.053 ± 0.146
5.332 ± 0.415
5.115 ± 0.242
2.294 ± 0.208
6.045 ± 0.228
7.347 ± 0.559
8.586 ± 0.392
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.232 ± 0.223
4.371 ± 0.243
3.72 ± 0.154
3.565 ± 0.2
4.278 ± 0.204
6.913 ± 0.145
7.44 ± 0.541
7.161 ± 0.277
1.085 ± 0.142
3.689 ± 0.311
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here