Sulfurovum sp. UBA12169
Average proteome isoelectric point is 6.54
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1860 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2D3WS76|A0A2D3WS76_9PROT Two-component system response regulator OS=Sulfurovum sp. UBA12169 OX=2015906 GN=CFH81_07960 PE=4 SV=1
MM1 pKa = 7.74 KK2 pKa = 9.02 KK3 pKa = 8.18 TLLLSVVASTMIMAGGDD20 pKa = 3.49 IAPVEE25 pKa = 4.32 PVVEE29 pKa = 4.79 TPAPAVSGWEE39 pKa = 4.06 FSGQAALYY47 pKa = 8.58 YY48 pKa = 8.49 QTHH51 pKa = 7.01 DD52 pKa = 3.2 AFGYY56 pKa = 10.61 SDD58 pKa = 6.04 LFDD61 pKa = 3.99 QDD63 pKa = 3.44 SSAANAGLQLRR74 pKa = 11.84 AVNADD79 pKa = 4.08 LYY81 pKa = 10.56 NGIGAGVEE89 pKa = 4.16 VSGLATFDD97 pKa = 4.23 LEE99 pKa = 4.26 NSVVSDD105 pKa = 3.17 VMQGFGTDD113 pKa = 3.89 DD114 pKa = 4.92 LDD116 pKa = 4.79 GGWISQMYY124 pKa = 8.19 LTYY127 pKa = 10.99 GFDD130 pKa = 3.29 NTSFKK135 pKa = 10.77 LGRR138 pKa = 11.84 QEE140 pKa = 4.22 LPKK143 pKa = 10.51 ALSPFAFSEE152 pKa = 4.03 EE153 pKa = 3.96 WNVFKK158 pKa = 10.37 NTYY161 pKa = 9.88 DD162 pKa = 3.24 ALLVVNSDD170 pKa = 2.98 ISNTTLVGAWVRR182 pKa = 11.84 SANQNGYY189 pKa = 9.98 GLGAMNGFNPSFNFQAGMPPILGTPASDD217 pKa = 3.0 MNSFEE222 pKa = 5.18 EE223 pKa = 4.07 INGDD227 pKa = 3.25 HH228 pKa = 6.43 GVFMLTAQNKK238 pKa = 9.58 SIEE241 pKa = 4.18 NLTLTGSWYY250 pKa = 10.46 YY251 pKa = 11.39 SNEE254 pKa = 3.45 FLTNDD259 pKa = 4.51 DD260 pKa = 5.95 LNILWGDD267 pKa = 3.35 AQYY270 pKa = 11.77 NFMQNYY276 pKa = 8.01 TLGLQGGTVMHH287 pKa = 7.25 DD288 pKa = 2.98 AFEE291 pKa = 4.49 TFNSGDD297 pKa = 3.47 DD298 pKa = 3.67 TVAFGAMIGGKK309 pKa = 9.88 YY310 pKa = 10.27 DD311 pKa = 3.29 IFHH314 pKa = 6.93 GALAFSSVDD323 pKa = 3.31 DD324 pKa = 4.16 GGFGVFNVGGQQTALYY340 pKa = 7.88 TQMMFNEE347 pKa = 4.08 RR348 pKa = 11.84 AIAFDD353 pKa = 3.54 SDD355 pKa = 3.36 TFMVKK360 pKa = 10.14 VGADD364 pKa = 3.25 VFGGKK369 pKa = 8.76 VCAAYY374 pKa = 10.29 GMSDD378 pKa = 3.25 MGMGDD383 pKa = 3.63 YY384 pKa = 11.47 NEE386 pKa = 4.96 FDD388 pKa = 3.51 LSYY391 pKa = 10.24 SAKK394 pKa = 9.05 VTDD397 pKa = 5.53 SIDD400 pKa = 3.11 LTAAYY405 pKa = 10.33 AFMDD409 pKa = 4.16 ADD411 pKa = 4.29 VEE413 pKa = 5.38 DD414 pKa = 5.68 FDD416 pKa = 5.04 DD417 pKa = 4.04 TNNVIRR423 pKa = 11.84 VIGRR427 pKa = 11.84 YY428 pKa = 8.34 NFF430 pKa = 3.39
Molecular weight: 46.51 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.702
IPC2_protein 3.745
IPC_protein 3.783
Toseland 3.541
ProMoST 3.948
Dawson 3.783
Bjellqvist 3.935
Wikipedia 3.732
Rodwell 3.605
Grimsley 3.452
Solomon 3.783
Lehninger 3.732
Nozaki 3.897
DTASelect 4.177
Thurlkill 3.605
EMBOSS 3.745
Sillero 3.91
Patrickios 0.846
IPC_peptide 3.77
IPC2_peptide 3.884
IPC2.peptide.svr19 3.8
Protein with the highest isoelectric point:
>tr|A0A2D3WHJ4|A0A2D3WHJ4_9PROT MFS transporter OS=Sulfurovum sp. UBA12169 OX=2015906 GN=CFH81_06545 PE=4 SV=1
MM1 pKa = 7.28 KK2 pKa = 9.39 RR3 pKa = 11.84 TYY5 pKa = 10.06 QPHH8 pKa = 4.56 TTPRR12 pKa = 11.84 KK13 pKa = 7.32 RR14 pKa = 11.84 THH16 pKa = 6.24 GFRR19 pKa = 11.84 TRR21 pKa = 11.84 MKK23 pKa = 8.87 TKK25 pKa = 10.11 NGRR28 pKa = 11.84 KK29 pKa = 9.33 VISARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.1 GRR39 pKa = 11.84 KK40 pKa = 8.77 KK41 pKa = 10.27 LAAA44 pKa = 4.49
Molecular weight: 5.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.391
IPC2_protein 11.052
IPC_protein 12.398
Toseland 12.559
ProMoST 13.042
Dawson 12.559
Bjellqvist 12.544
Wikipedia 13.027
Rodwell 12.384
Grimsley 12.603
Solomon 13.056
Lehninger 12.954
Nozaki 12.559
DTASelect 12.544
Thurlkill 12.559
EMBOSS 13.056
Sillero 12.559
Patrickios 12.106
IPC_peptide 13.056
IPC2_peptide 12.032
IPC2.peptide.svr19 8.993
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1860
0
1860
564002
37
1807
303.2
34.05
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.87 ± 0.056
0.939 ± 0.023
5.403 ± 0.042
7.136 ± 0.054
4.749 ± 0.047
6.551 ± 0.043
2.132 ± 0.025
7.959 ± 0.054
7.957 ± 0.054
9.607 ± 0.067
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.819 ± 0.029
4.3 ± 0.04
3.311 ± 0.034
3.34 ± 0.032
3.791 ± 0.036
6.0 ± 0.042
5.195 ± 0.046
6.275 ± 0.046
0.844 ± 0.018
3.821 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here