Klebsiella phage Kund-ULIP54
Average proteome isoelectric point is 6.56
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 46 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P6PHU1|A0A4P6PHU1_9CAUD Uncharacterized protein OS=Klebsiella phage Kund-ULIP54 OX=2307017 GN=D3A57_0013 PE=4 SV=1
MM1 pKa = 5.82 VTYY4 pKa = 9.61 YY5 pKa = 11.12 VYY7 pKa = 10.86 VNQMEE12 pKa = 4.51 YY13 pKa = 11.07 FEE15 pKa = 6.82 ADD17 pKa = 3.33 TLEE20 pKa = 4.08 EE21 pKa = 4.75 AIRR24 pKa = 11.84 LCDD27 pKa = 3.87 SVPGSHH33 pKa = 6.89 VEE35 pKa = 4.2 DD36 pKa = 3.71 SVLDD40 pKa = 3.69 EE41 pKa = 4.3 VIYY44 pKa = 10.88 ANRR47 pKa = 3.66
Molecular weight: 5.48 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.924
IPC2_protein 3.999
IPC_protein 3.821
Toseland 3.656
ProMoST 3.986
Dawson 3.795
Bjellqvist 3.961
Wikipedia 3.719
Rodwell 3.668
Grimsley 3.579
Solomon 3.757
Lehninger 3.719
Nozaki 3.935
DTASelect 4.05
Thurlkill 3.719
EMBOSS 3.732
Sillero 3.935
Patrickios 0.121
IPC_peptide 3.77
IPC2_peptide 3.91
IPC2.peptide.svr19 3.85
Protein with the highest isoelectric point:
>tr|A0A4P6PMT4|A0A4P6PMT4_9CAUD Uncharacterized protein OS=Klebsiella phage Kund-ULIP54 OX=2307017 GN=D3A57_0015 PE=4 SV=1
MM1 pKa = 7.29 SQRR4 pKa = 11.84 TNLKK8 pKa = 9.79 KK9 pKa = 10.34 AFKK12 pKa = 8.77 IARR15 pKa = 11.84 MVTSYY20 pKa = 11.38 GAGEE24 pKa = 4.19 KK25 pKa = 8.87 RR26 pKa = 11.84 TKK28 pKa = 10.51 RR29 pKa = 11.84 ILAASAGRR37 pKa = 11.84 LPARR41 pKa = 11.84 HH42 pKa = 6.32 RR43 pKa = 11.84 KK44 pKa = 8.1 WVMQQVYY51 pKa = 9.5 VEE53 pKa = 4.94 LYY55 pKa = 8.87 HH56 pKa = 6.92 PEE58 pKa = 4.93 RR59 pKa = 11.84 IPDD62 pKa = 4.01 GYY64 pKa = 10.13 IWSPVGWKK72 pKa = 9.97 RR73 pKa = 11.84 VATNN77 pKa = 3.3
Molecular weight: 8.96 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.28
IPC2_protein 10.218
IPC_protein 11.125
Toseland 11.184
ProMoST 11.199
Dawson 11.242
Bjellqvist 11.052
Wikipedia 11.55
Rodwell 11.403
Grimsley 11.286
Solomon 11.491
Lehninger 11.433
Nozaki 11.155
DTASelect 11.052
Thurlkill 11.184
EMBOSS 11.623
Sillero 11.199
Patrickios 11.155
IPC_peptide 11.506
IPC2_peptide 10.175
IPC2.peptide.svr19 8.204
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
46
0
46
12426
40
1321
270.1
30.04
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.062 ± 0.528
0.934 ± 0.154
6.293 ± 0.18
7.09 ± 0.46
3.589 ± 0.169
7.951 ± 0.396
1.714 ± 0.23
4.861 ± 0.178
6.599 ± 0.383
8.048 ± 0.326
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.599 ± 0.16
4.201 ± 0.271
3.694 ± 0.167
4.338 ± 0.341
5.633 ± 0.254
6.06 ± 0.348
5.746 ± 0.31
6.784 ± 0.369
1.392 ± 0.187
3.412 ± 0.21
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here