Arthronema virus TR020
Average proteome isoelectric point is 5.96
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 55 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A7G5AWZ8|A0A7G5AWZ8_9CAUD Putative endopeptidase OS=Arthronema virus TR020 OX=2736280 PE=4 SV=1
MM1 pKa = 7.37 PNSVRR6 pKa = 11.84 VLWDD10 pKa = 3.58 DD11 pKa = 4.6 DD12 pKa = 3.45 FTGYY16 pKa = 7.57 VTCDD20 pKa = 3.42 LQDD23 pKa = 3.38 GTLHH27 pKa = 6.01 YY28 pKa = 11.31 GEE30 pKa = 5.02 VPLEE34 pKa = 3.63 LMTYY38 pKa = 9.56 HH39 pKa = 6.19 NQPIEE44 pKa = 4.95 DD45 pKa = 4.34 IYY47 pKa = 11.62 DD48 pKa = 3.83 DD49 pKa = 4.04 YY50 pKa = 12.25
Molecular weight: 5.9 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.891
IPC2_protein 3.668
IPC_protein 3.592
Toseland 3.389
ProMoST 3.808
Dawson 3.617
Bjellqvist 3.783
Wikipedia 3.617
Rodwell 3.439
Grimsley 3.312
Solomon 3.579
Lehninger 3.541
Nozaki 3.77
DTASelect 3.999
Thurlkill 3.49
EMBOSS 3.617
Sillero 3.732
Patrickios 0.006
IPC_peptide 3.567
IPC2_peptide 3.694
IPC2.peptide.svr19 3.698
Protein with the highest isoelectric point:
>tr|A0A7G3WH16|A0A7G3WH16_9CAUD Uncharacterized protein OS=Arthronema virus TR020 OX=2736280 PE=4 SV=1
MM1 pKa = 6.73 NTRR4 pKa = 11.84 VLLTFLVFLMFSAHH18 pKa = 6.38 AALALRR24 pKa = 11.84 HH25 pKa = 5.67 MLGSHH30 pKa = 6.31 LQRR33 pKa = 11.84 LNTIEE38 pKa = 3.8 QSEE41 pKa = 4.49 RR42 pKa = 11.84 KK43 pKa = 8.68 HH44 pKa = 4.91 QQ45 pKa = 3.63
Molecular weight: 5.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.161
IPC2_protein 9.853
IPC_protein 11.374
Toseland 11.564
ProMoST 12.032
Dawson 11.564
Bjellqvist 11.535
Wikipedia 12.018
Rodwell 11.33
Grimsley 11.608
Solomon 12.047
Lehninger 11.945
Nozaki 11.564
DTASelect 11.535
Thurlkill 11.564
EMBOSS 12.047
Sillero 11.564
Patrickios 11.199
IPC_peptide 12.047
IPC2_peptide 11.023
IPC2.peptide.svr19 9.579
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
55
0
55
13728
31
1506
249.6
28.01
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.561 ± 0.374
0.386 ± 0.095
6.119 ± 0.259
6.52 ± 0.399
3.795 ± 0.246
6.439 ± 0.243
1.632 ± 0.199
4.939 ± 0.245
4.924 ± 0.273
9.521 ± 0.297
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.426 ± 0.221
5.507 ± 0.259
4.159 ± 0.392
4.538 ± 0.378
5.704 ± 0.259
6.6 ± 0.376
6.993 ± 0.431
7.059 ± 0.327
1.151 ± 0.124
4.028 ± 0.293
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here