Cafeteria roenbergensis virus (strain BV-PW1) (CroV)

Taxonomy: Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Megaviricetes; Imitervirales; Mimiviridae; Cafeteriavirus; Cafeteria roenbergensis virus

Average proteome isoelectric point is 7.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 544 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E3T4M9|E3T4M9_CROVB ATP-dependent helicase VETFS OS=Cafeteria roenbergensis virus (strain BV-PW1) OX=693272 GN=crov109 PE=3 SV=1
MM1 pKa = 6.99FFEE4 pKa = 5.19YY5 pKa = 9.17ITLTHH10 pKa = 7.19LAQFALKK17 pKa = 10.13HH18 pKa = 6.26HH19 pKa = 6.68ILSDD23 pKa = 3.47FWYY26 pKa = 8.33MGLSIFEE33 pKa = 4.13YY34 pKa = 10.45LFRR37 pKa = 11.84TIFIGMLLPINLYY50 pKa = 10.15GNRR53 pKa = 11.84LAFIKK58 pKa = 9.78FIIIIILLLNSFNNGLDD75 pKa = 3.63FYY77 pKa = 11.5FNDD80 pKa = 3.58TFGFYY85 pKa = 10.38PFQAVFGFWIIGLFINSIINNIISSTQTDD114 pKa = 4.44RR115 pKa = 11.84INYY118 pKa = 8.78DD119 pKa = 3.17NNTYY123 pKa = 10.15FYY125 pKa = 11.34DD126 pKa = 4.03MEE128 pKa = 6.61DD129 pKa = 3.46STIKK133 pKa = 8.87PTNISTEE140 pKa = 3.85EE141 pKa = 3.94STEE144 pKa = 3.89EE145 pKa = 3.81STEE148 pKa = 3.93EE149 pKa = 3.78STEE152 pKa = 3.92EE153 pKa = 3.78LNEE156 pKa = 3.96EE157 pKa = 4.28SNEE160 pKa = 3.89EE161 pKa = 3.99STEE164 pKa = 3.89EE165 pKa = 3.88STEE168 pKa = 3.89EE169 pKa = 4.0SIEE172 pKa = 4.05EE173 pKa = 4.2STEE176 pKa = 3.77EE177 pKa = 4.02TTEE180 pKa = 3.89EE181 pKa = 4.0TTEE184 pKa = 3.99EE185 pKa = 4.06SSEE188 pKa = 4.1KK189 pKa = 10.79LNEE192 pKa = 3.85

Molecular weight: 22.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E3T5V9|E3T5V9_CROVB Uncharacterized protein OS=Cafeteria roenbergensis virus (strain BV-PW1) OX=693272 GN=crov538 PE=4 SV=1
MM1 pKa = 8.07PYY3 pKa = 9.19TRR5 pKa = 11.84KK6 pKa = 10.45NNTRR10 pKa = 11.84NSKK13 pKa = 9.32HH14 pKa = 6.44RR15 pKa = 11.84KK16 pKa = 6.59PKK18 pKa = 10.41KK19 pKa = 9.85NLFQKK24 pKa = 10.52RR25 pKa = 11.84LSKK28 pKa = 10.55EE29 pKa = 4.0FYY31 pKa = 10.55EE32 pKa = 4.4SFTYY36 pKa = 10.39DD37 pKa = 2.7SYY39 pKa = 11.29LAQMTSNPKK48 pKa = 10.15LFSNLFMMKK57 pKa = 9.84LRR59 pKa = 11.84SGFPPSQNILKK70 pKa = 10.24RR71 pKa = 11.84VMEE74 pKa = 4.47SKK76 pKa = 10.83NPDD79 pKa = 3.3MQPVRR84 pKa = 11.84NFIEE88 pKa = 4.23YY89 pKa = 10.93NRR91 pKa = 11.84MQFKK95 pKa = 10.46NN96 pKa = 3.46

Molecular weight: 11.71 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

544

0

544

184963

47

3337

340.0

39.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

2.592 ± 0.125

1.249 ± 0.048

5.698 ± 0.089

6.099 ± 0.088

5.145 ± 0.083

3.834 ± 0.096

1.94 ± 0.045

10.21 ± 0.139

10.383 ± 0.137

9.581 ± 0.107

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.936 ± 0.053

9.741 ± 0.183

3.513 ± 0.084

3.216 ± 0.056

2.388 ± 0.067

6.333 ± 0.102

5.856 ± 0.094

4.051 ± 0.071

0.907 ± 0.043

5.327 ± 0.087
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski