Erysipelothrix larvae
Average proteome isoelectric point is 6.03
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2225 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0X8GZ37|A0A0X8GZ37_9FIRM Polyprenyl glycosylphosphotransferase OS=Erysipelothrix larvae OX=1514105 GN=AOC36_02510 PE=3 SV=1
MM1 pKa = 7.67 LLLLLIITGCAQSNPSTDD19 pKa = 2.88 ATGFLCEE26 pKa = 4.09 KK27 pKa = 9.98 QDD29 pKa = 3.67 YY30 pKa = 10.51 GLVEE34 pKa = 4.26 VRR36 pKa = 11.84 EE37 pKa = 4.9 LILDD41 pKa = 3.47 SDD43 pKa = 4.1 GTIKK47 pKa = 10.86 KK48 pKa = 10.1 LISTSRR54 pKa = 11.84 IEE56 pKa = 4.16 FDD58 pKa = 3.24 ASQSADD64 pKa = 3.4 VTIEE68 pKa = 4.09 DD69 pKa = 3.73 YY70 pKa = 11.81 QMFLAQLYY78 pKa = 8.9 EE79 pKa = 4.37 SLVSQDD85 pKa = 4.32 DD86 pKa = 4.63 GINYY90 pKa = 9.78 SSQIIEE96 pKa = 4.51 NIIEE100 pKa = 4.28 VIMTFDD106 pKa = 3.46 MVQISDD112 pKa = 3.93 STLVWLGLPIEE123 pKa = 4.33 NTQNRR128 pKa = 11.84 IIVEE132 pKa = 3.8 QYY134 pKa = 10.53 IDD136 pKa = 3.57 NLEE139 pKa = 4.37 SNGYY143 pKa = 6.99 QCKK146 pKa = 7.66 TTTATLGG153 pKa = 3.4
Molecular weight: 17.07 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.724
IPC2_protein 3.795
IPC_protein 3.745
Toseland 3.541
ProMoST 3.91
Dawson 3.732
Bjellqvist 3.897
Wikipedia 3.668
Rodwell 3.579
Grimsley 3.465
Solomon 3.719
Lehninger 3.668
Nozaki 3.859
DTASelect 4.05
Thurlkill 3.605
EMBOSS 3.668
Sillero 3.872
Patrickios 0.896
IPC_peptide 3.719
IPC2_peptide 3.846
IPC2.peptide.svr19 3.786
Protein with the highest isoelectric point:
>tr|A0A109UHS8|A0A109UHS8_9FIRM Uncharacterized protein OS=Erysipelothrix larvae OX=1514105 GN=AOC36_11715 PE=4 SV=1
MM1 pKa = 7.29 NFAPIFGLIALSMMFFVIGSLRR23 pKa = 11.84 NINVLFGLKK32 pKa = 10.25 AILNQSVVVAIVATGAIFIYY52 pKa = 10.62 SLGSFDD58 pKa = 3.66 ISLGASVTVSALLGAITFNATNSILLMFLVCILVAVSIALFNSVLASIFNLPVFVTTIAMLSVLNAIVLLLISVNGTGSTVSISASSVQNLNTEE152 pKa = 3.98 WFKK155 pKa = 10.98 IATLMVFAGLCTFLFNFTRR174 pKa = 11.84 LGRR177 pKa = 11.84 MQKK180 pKa = 10.49 FIGGNPICARR190 pKa = 11.84 LTGISSKK197 pKa = 10.33 KK198 pKa = 9.7 ISIISFALSGLGVGIGAFLLIVYY221 pKa = 10.05 APTLTRR227 pKa = 11.84 NSASSVGMDD236 pKa = 3.32 VIIAIVFGGMPISGGARR253 pKa = 11.84 SKK255 pKa = 10.38 IYY257 pKa = 10.37 SAIVGSFSMTFLSQIMVMFNMNSGINQIVKK287 pKa = 10.48 AIIFILVVYY296 pKa = 9.84 LATSNQRR303 pKa = 11.84 RR304 pKa = 11.84 KK305 pKa = 9.85 ILPRR309 pKa = 4.54
Molecular weight: 32.73 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.333
IPC2_protein 9.97
IPC_protein 10.687
Toseland 10.833
ProMoST 10.73
Dawson 10.921
Bjellqvist 10.628
Wikipedia 11.125
Rodwell 11.257
Grimsley 10.979
Solomon 11.023
Lehninger 10.994
Nozaki 10.818
DTASelect 10.613
Thurlkill 10.833
EMBOSS 11.228
Sillero 10.862
Patrickios 11.008
IPC_peptide 11.038
IPC2_peptide 9.721
IPC2.peptide.svr19 8.364
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2225
0
2225
709240
46
4515
318.8
35.88
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.125 ± 0.045
0.908 ± 0.018
6.025 ± 0.043
6.435 ± 0.057
4.599 ± 0.037
6.298 ± 0.05
2.213 ± 0.026
8.259 ± 0.05
6.246 ± 0.047
9.572 ± 0.058
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.75 ± 0.029
5.114 ± 0.043
3.159 ± 0.032
3.54 ± 0.03
3.913 ± 0.042
6.672 ± 0.046
5.938 ± 0.076
7.357 ± 0.05
0.794 ± 0.018
4.084 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here