[Collinsella] massiliensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Coriobacteriia; Coriobacteriales; Coriobacteriaceae; Enorma

Average proteome isoelectric point is 5.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1896 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Y3XZ00|A0A1Y3XZ00_9ACTN Uncharacterized protein OS=[Collinsella] massiliensis OX=1232426 GN=B5G02_06680 PE=4 SV=1
MM1 pKa = 6.6YY2 pKa = 10.09QPSISRR8 pKa = 11.84RR9 pKa = 11.84GLIATLAAASALPLAACGSEE29 pKa = 3.99DD30 pKa = 3.47AKK32 pKa = 11.0EE33 pKa = 4.24GSSTGKK39 pKa = 10.58ASASDD44 pKa = 3.01GKK46 pKa = 8.9TYY48 pKa = 10.58KK49 pKa = 10.26IGVLQLTQHH58 pKa = 6.84AALDD62 pKa = 3.68QTNEE66 pKa = 3.9GFIKK70 pKa = 10.76ALDD73 pKa = 3.98EE74 pKa = 5.03AGLSYY79 pKa = 11.16DD80 pKa = 4.79ADD82 pKa = 3.67QQNASNDD89 pKa = 3.32QTACQTIAQTFVNDD103 pKa = 3.79GSDD106 pKa = 3.79LIFAIGTPAAQAVAAATKK124 pKa = 8.64EE125 pKa = 3.91IPIVGSAITDD135 pKa = 4.17FEE137 pKa = 4.88SVGLVSSNDD146 pKa = 3.36APGGNVTGSSDD157 pKa = 3.75LTPVSDD163 pKa = 5.32QIDD166 pKa = 4.33LLVKK170 pKa = 10.0LVPDD174 pKa = 4.62CKK176 pKa = 10.19TAGILYY182 pKa = 7.7CTAEE186 pKa = 4.46SNSTIQVDD194 pKa = 3.94LAKK197 pKa = 10.28EE198 pKa = 3.83ALEE201 pKa = 5.1SYY203 pKa = 10.44DD204 pKa = 3.75LPYY207 pKa = 11.3EE208 pKa = 4.13EE209 pKa = 5.07FTVSSSNEE217 pKa = 3.62IQSVVEE223 pKa = 3.98TAVGKK228 pKa = 10.57VDD230 pKa = 5.84AIYY233 pKa = 10.77APTDD237 pKa = 3.38NTIAAAMAQVSSIADD252 pKa = 3.51EE253 pKa = 4.64AGVPVIVGCDD263 pKa = 3.28TMVEE267 pKa = 4.21DD268 pKa = 5.55GGTASYY274 pKa = 10.36SINYY278 pKa = 8.85VDD280 pKa = 4.96LGHH283 pKa = 7.19KK284 pKa = 9.68AGEE287 pKa = 3.98MAVRR291 pKa = 11.84ILTEE295 pKa = 4.16GEE297 pKa = 4.16NPADD301 pKa = 3.75MPIEE305 pKa = 3.94YY306 pKa = 10.01LDD308 pKa = 4.33ASEE311 pKa = 4.73CTLIANQASADD322 pKa = 3.61ACGVDD327 pKa = 5.94LGILDD332 pKa = 3.64NPKK335 pKa = 9.85IVV337 pKa = 3.15

Molecular weight: 34.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Y3XW37|A0A1Y3XW37_9ACTN Transcription termination factor Rho OS=[Collinsella] massiliensis OX=1232426 GN=rho PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.42RR3 pKa = 11.84TYY5 pKa = 10.34QPNKK9 pKa = 8.62RR10 pKa = 11.84KK11 pKa = 9.56RR12 pKa = 11.84AKK14 pKa = 8.48THH16 pKa = 5.36GFRR19 pKa = 11.84SRR21 pKa = 11.84MATKK25 pKa = 10.37GGRR28 pKa = 11.84AVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.73GRR39 pKa = 11.84KK40 pKa = 8.88RR41 pKa = 11.84LTVV44 pKa = 3.11

Molecular weight: 5.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1896

0

1896

653081

31

3985

344.5

37.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.496 ± 0.083

1.482 ± 0.026

6.389 ± 0.055

6.846 ± 0.052

3.608 ± 0.033

8.256 ± 0.049

1.919 ± 0.027

5.186 ± 0.049

3.058 ± 0.054

9.171 ± 0.078

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.59 ± 0.028

2.582 ± 0.043

4.489 ± 0.036

2.777 ± 0.031

6.558 ± 0.082

5.396 ± 0.043

5.44 ± 0.088

7.903 ± 0.049

1.016 ± 0.021

2.84 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski