Siculibacillus lacustris

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Ancalomicrobiaceae; Siculibacillus

Average proteome isoelectric point is 6.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4425 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Q9VL92|A0A4Q9VL92_9RHIZ YkuD domain-containing protein OS=Siculibacillus lacustris OX=1549641 GN=EYW49_14175 PE=3 SV=1
MM1 pKa = 8.1DD2 pKa = 4.95GPVDD6 pKa = 3.64EE7 pKa = 6.32AIGGDD12 pKa = 3.59PCDD15 pKa = 4.56PYY17 pKa = 11.71DD18 pKa = 5.0DD19 pKa = 4.05GVIEE23 pKa = 4.64IRR25 pKa = 11.84VTDD28 pKa = 3.93FAGEE32 pKa = 3.9VHH34 pKa = 6.71ALKK37 pKa = 10.69AIDD40 pKa = 3.48GWRR43 pKa = 11.84VMEE46 pKa = 5.17VIRR49 pKa = 11.84DD50 pKa = 3.14WGLPIKK56 pKa = 10.33AEE58 pKa = 4.16CGGACACGTCHH69 pKa = 7.09VYY71 pKa = 10.6VDD73 pKa = 4.2PQWVDD78 pKa = 3.35KK79 pKa = 11.01LPPPTDD85 pKa = 3.34EE86 pKa = 6.3EE87 pKa = 4.31MDD89 pKa = 3.65QLDD92 pKa = 3.45QIYY95 pKa = 10.22AVQEE99 pKa = 3.9NSRR102 pKa = 11.84LSCQILTAQEE112 pKa = 3.98LDD114 pKa = 3.61GLEE117 pKa = 4.22LQLAPGSEE125 pKa = 4.3VDD127 pKa = 3.51GG128 pKa = 4.44

Molecular weight: 13.9 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Q9VUI5|A0A4Q9VUI5_9RHIZ Putative manganese efflux pump MntP OS=Siculibacillus lacustris OX=1549641 GN=mntP PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.09QPSKK9 pKa = 8.8IVRR12 pKa = 11.84KK13 pKa = 9.24RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.56GFRR19 pKa = 11.84ARR21 pKa = 11.84SATVGGRR28 pKa = 11.84VVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 8.98RR41 pKa = 11.84LSAA44 pKa = 4.03

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4425

0

4425

1441686

40

3455

325.8

34.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.221 ± 0.056

0.786 ± 0.012

5.88 ± 0.03

5.299 ± 0.033

3.593 ± 0.023

8.936 ± 0.045

1.994 ± 0.017

4.939 ± 0.027

2.633 ± 0.035

10.147 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.113 ± 0.016

1.945 ± 0.02

5.453 ± 0.03

2.307 ± 0.022

7.828 ± 0.05

4.821 ± 0.03

5.644 ± 0.049

8.354 ± 0.031

1.316 ± 0.017

1.787 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski