Streptococcus phage Javan143

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 55 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZZS8|A0A4D5ZZS8_9CAUD Integrase OS=Streptococcus phage Javan143 OX=2548004 GN=Javan143_0028 PE=3 SV=1
MM1 pKa = 6.97NWKK4 pKa = 8.45HH5 pKa = 5.42TYY7 pKa = 9.79LRR9 pKa = 11.84NATDD13 pKa = 3.5EE14 pKa = 4.29EE15 pKa = 4.49KK16 pKa = 10.9KK17 pKa = 10.79EE18 pKa = 4.08LGCEE22 pKa = 4.15EE23 pKa = 4.13IWDD26 pKa = 4.18GEE28 pKa = 4.47LPCEE32 pKa = 4.34DD33 pKa = 3.91NQILVYY39 pKa = 10.57KK40 pKa = 10.28NGYY43 pKa = 7.52FTIDD47 pKa = 3.22DD48 pKa = 3.91WVNDD52 pKa = 4.01LGEE55 pKa = 4.85CGLTEE60 pKa = 4.65NYY62 pKa = 10.64DD63 pKa = 3.23FTDD66 pKa = 4.79FYY68 pKa = 10.44WCEE71 pKa = 3.81PEE73 pKa = 5.48APDD76 pKa = 4.73VGKK79 pKa = 10.64

Molecular weight: 9.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6A0V4|A0A4D6A0V4_9CAUD Clp protease-like protein OS=Streptococcus phage Javan143 OX=2548004 GN=Javan143_0034 PE=3 SV=1
MM1 pKa = 7.92EE2 pKa = 6.22DD3 pKa = 3.86NEE5 pKa = 4.41TCIARR10 pKa = 11.84LKK12 pKa = 10.26QSNIEE17 pKa = 4.01LTQKK21 pKa = 10.34VVDD24 pKa = 4.17VTWKK28 pKa = 10.59DD29 pKa = 3.23MRR31 pKa = 11.84RR32 pKa = 11.84VARR35 pKa = 11.84ARR37 pKa = 11.84LSRR40 pKa = 11.84RR41 pKa = 11.84KK42 pKa = 10.01YY43 pKa = 9.5GVKK46 pKa = 9.84IGG48 pKa = 3.54

Molecular weight: 5.66 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

55

0

55

11559

38

1366

210.2

23.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.618 ± 0.612

0.51 ± 0.101

6.843 ± 0.377

7.873 ± 0.346

4.023 ± 0.266

5.961 ± 0.299

1.367 ± 0.138

7.016 ± 0.319

9.127 ± 0.375

8.695 ± 0.365

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.362 ± 0.164

5.234 ± 0.199

2.535 ± 0.155

4.378 ± 0.24

4.542 ± 0.26

6.298 ± 0.351

5.606 ± 0.294

6.056 ± 0.281

1.055 ± 0.102

3.902 ± 0.368
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski