Lactococcus phage bIL311

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 7.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 21 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q9AZH7|Q9AZH7_9CAUD Orf4 OS=Lactococcus phage bIL311 OX=151534 GN=orf4 PE=4 SV=1
MM1 pKa = 7.59TEE3 pKa = 5.23FITIDD8 pKa = 3.37EE9 pKa = 4.53AKK11 pKa = 10.81LLFTEE16 pKa = 5.02CLAKK20 pKa = 10.22SRR22 pKa = 11.84HH23 pKa = 6.12HH24 pKa = 7.59IEE26 pKa = 3.96VDD28 pKa = 3.41DD29 pKa = 4.12VTDD32 pKa = 3.6KK33 pKa = 11.54LFDD36 pKa = 4.14GKK38 pKa = 10.91SVATFTFMGNGYY50 pKa = 7.75QVEE53 pKa = 4.38LEE55 pKa = 4.1AA56 pKa = 6.66

Molecular weight: 6.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q9AZG9|Q9AZG9_9CAUD Orf12 OS=Lactococcus phage bIL311 OX=151534 GN=orf12 PE=4 SV=1
MM1 pKa = 7.58LWQLKK6 pKa = 8.11SQNVGHH12 pKa = 7.28LVQYY16 pKa = 10.29QNNKK20 pKa = 8.77GEE22 pKa = 4.33IMRR25 pKa = 11.84VRR27 pKa = 11.84HH28 pKa = 5.6PPKK31 pKa = 10.55SVVLTHH37 pKa = 6.88LL38 pKa = 3.89

Molecular weight: 4.48 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

21

0

21

2927

38

489

139.4

16.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.535 ± 0.406

0.478 ± 0.204

5.364 ± 0.498

8.2 ± 0.565

4.681 ± 0.516

4.612 ± 0.363

2.05 ± 0.191

7.687 ± 0.448

10.489 ± 0.577

9.122 ± 0.512

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.597 ± 0.214

5.91 ± 0.462

2.221 ± 0.261

3.519 ± 0.417

4.851 ± 0.47

7.209 ± 0.65

5.159 ± 0.369

5.364 ± 0.432

1.093 ± 0.145

3.861 ± 0.295
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski