Amino acid dipepetide frequency for Lactococcus phage bIL311

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.734AlaAla: 2.734 ± 0.654
0.684AlaCys: 0.684 ± 0.626
3.076AlaAsp: 3.076 ± 1.448
7.519AlaGlu: 7.519 ± 2.078
3.076AlaPhe: 3.076 ± 0.784
2.392AlaGly: 2.392 ± 0.754
1.025AlaHis: 1.025 ± 0.459
3.076AlaIle: 3.076 ± 0.988
5.126AlaLys: 5.126 ± 1.159
5.81AlaLeu: 5.81 ± 0.967
2.392AlaMet: 2.392 ± 0.75
2.051AlaAsn: 2.051 ± 0.612
1.709AlaPro: 1.709 ± 0.573
1.709AlaGln: 1.709 ± 0.601
2.734AlaArg: 2.734 ± 1.492
3.076AlaSer: 3.076 ± 0.747
1.709AlaThr: 1.709 ± 0.973
3.076AlaVal: 3.076 ± 0.768
1.367AlaTrp: 1.367 ± 0.784
1.025AlaTyr: 1.025 ± 0.444
0.0AlaXaa: 0.0 ± 0.0
Cys
0.0CysAla: 0.0 ± 0.0
0.0CysCys: 0.0 ± 0.0
0.0CysAsp: 0.0 ± 0.0
0.0CysGlu: 0.0 ± 0.0
0.0CysPhe: 0.0 ± 0.0
0.0CysGly: 0.0 ± 0.0
0.342CysHis: 0.342 ± 0.313
0.0CysIle: 0.0 ± 0.0
1.025CysLys: 1.025 ± 0.726
1.025CysLeu: 1.025 ± 0.52
0.0CysMet: 0.0 ± 0.0
0.0CysAsn: 0.0 ± 0.0
0.0CysPro: 0.0 ± 0.0
0.684CysGln: 0.684 ± 0.36
0.684CysArg: 0.684 ± 0.417
0.684CysSer: 0.684 ± 0.417
0.0CysThr: 0.0 ± 0.0
0.0CysVal: 0.0 ± 0.0
0.0CysTrp: 0.0 ± 0.0
0.342CysTyr: 0.342 ± 0.313
0.0CysXaa: 0.0 ± 0.0
Asp
1.709AspAla: 1.709 ± 0.75
0.684AspCys: 0.684 ± 0.368
4.443AspAsp: 4.443 ± 0.965
4.785AspGlu: 4.785 ± 1.29
2.051AspPhe: 2.051 ± 0.866
3.076AspGly: 3.076 ± 1.07
0.684AspHis: 0.684 ± 0.525
4.101AspIle: 4.101 ± 1.005
7.519AspLys: 7.519 ± 1.58
6.152AspLeu: 6.152 ± 1.713
0.342AspMet: 0.342 ± 0.287
3.076AspAsn: 3.076 ± 0.838
1.025AspPro: 1.025 ± 0.519
1.025AspGln: 1.025 ± 0.457
0.684AspArg: 0.684 ± 0.51
3.076AspSer: 3.076 ± 0.992
2.392AspThr: 2.392 ± 0.815
2.051AspVal: 2.051 ± 0.74
1.025AspTrp: 1.025 ± 0.488
3.759AspTyr: 3.759 ± 0.714
0.0AspXaa: 0.0 ± 0.0
Glu
6.152GluAla: 6.152 ± 1.252
1.025GluCys: 1.025 ± 0.534
3.418GluAsp: 3.418 ± 1.097
5.468GluGlu: 5.468 ± 1.721
3.076GluPhe: 3.076 ± 1.18
1.367GluGly: 1.367 ± 0.683
0.684GluHis: 0.684 ± 0.465
7.177GluIle: 7.177 ± 1.384
6.152GluLys: 6.152 ± 1.321
8.544GluLeu: 8.544 ± 2.437
3.076GluMet: 3.076 ± 1.029
3.076GluAsn: 3.076 ± 0.851
1.709GluPro: 1.709 ± 0.806
3.418GluGln: 3.418 ± 1.034
6.494GluArg: 6.494 ± 1.956
6.835GluSer: 6.835 ± 1.093
4.443GluThr: 4.443 ± 1.229
4.785GluVal: 4.785 ± 1.012
0.684GluTrp: 0.684 ± 0.541
4.443GluTyr: 4.443 ± 0.613
0.0GluXaa: 0.0 ± 0.0
Phe
1.025PheAla: 1.025 ± 0.529
0.0PheCys: 0.0 ± 0.0
5.126PheAsp: 5.126 ± 1.136
5.81PheGlu: 5.81 ± 1.765
1.367PhePhe: 1.367 ± 0.653
2.392PheGly: 2.392 ± 0.64
1.025PheHis: 1.025 ± 0.554
4.101PheIle: 4.101 ± 1.582
4.443PheLys: 4.443 ± 0.977
2.734PheLeu: 2.734 ± 1.033
1.709PheMet: 1.709 ± 0.86
3.076PheAsn: 3.076 ± 1.468
1.367PhePro: 1.367 ± 0.69
2.051PheGln: 2.051 ± 1.184
1.367PheArg: 1.367 ± 0.722
2.392PheSer: 2.392 ± 0.851
3.076PheThr: 3.076 ± 1.113
2.392PheVal: 2.392 ± 0.944
0.0PheTrp: 0.0 ± 0.0
1.025PheTyr: 1.025 ± 0.382
0.0PheXaa: 0.0 ± 0.0
Gly
2.392GlyAla: 2.392 ± 0.485
0.0GlyCys: 0.0 ± 0.0
3.076GlyAsp: 3.076 ± 1.16
4.101GlyGlu: 4.101 ± 1.338
2.051GlyPhe: 2.051 ± 0.711
2.051GlyGly: 2.051 ± 0.784
1.367GlyHis: 1.367 ± 0.637
2.392GlyIle: 2.392 ± 0.607
5.126GlyLys: 5.126 ± 1.091
3.076GlyLeu: 3.076 ± 0.961
1.367GlyMet: 1.367 ± 0.715
4.443GlyAsn: 4.443 ± 1.269
1.025GlyPro: 1.025 ± 0.516
2.051GlyGln: 2.051 ± 0.705
1.709GlyArg: 1.709 ± 0.557
2.392GlySer: 2.392 ± 0.905
2.734GlyThr: 2.734 ± 0.822
3.076GlyVal: 3.076 ± 0.903
0.0GlyTrp: 0.0 ± 0.0
1.709GlyTyr: 1.709 ± 0.709
0.0GlyXaa: 0.0 ± 0.0
His
1.367HisAla: 1.367 ± 0.682
0.0HisCys: 0.0 ± 0.0
0.342HisAsp: 0.342 ± 0.313
0.684HisGlu: 0.684 ± 0.456
1.367HisPhe: 1.367 ± 0.681
3.418HisGly: 3.418 ± 0.828
0.684HisHis: 0.684 ± 0.445
1.367HisIle: 1.367 ± 0.576
1.709HisLys: 1.709 ± 0.689
2.051HisLeu: 2.051 ± 0.834
0.0HisMet: 0.0 ± 0.0
1.367HisAsn: 1.367 ± 0.416
0.342HisPro: 0.342 ± 0.359
1.025HisGln: 1.025 ± 0.592
0.342HisArg: 0.342 ± 0.313
0.684HisSer: 0.684 ± 0.403
1.025HisThr: 1.025 ± 0.654
1.367HisVal: 1.367 ± 0.591
0.342HisTrp: 0.342 ± 0.324
1.025HisTyr: 1.025 ± 0.638
0.0HisXaa: 0.0 ± 0.0
Ile
4.443IleAla: 4.443 ± 1.535
0.0IleCys: 0.0 ± 0.0
4.101IleAsp: 4.101 ± 1.158
6.835IleGlu: 6.835 ± 1.347
2.392IlePhe: 2.392 ± 0.94
5.468IleGly: 5.468 ± 1.047
0.342IleHis: 0.342 ± 0.307
5.468IleIle: 5.468 ± 1.312
10.253IleLys: 10.253 ± 1.585
7.861IleLeu: 7.861 ± 2.612
1.709IleMet: 1.709 ± 0.656
4.443IleAsn: 4.443 ± 0.776
2.051IlePro: 2.051 ± 0.895
1.709IleGln: 1.709 ± 0.91
4.101IleArg: 4.101 ± 0.988
6.835IleSer: 6.835 ± 1.157
2.734IleThr: 2.734 ± 1.046
2.734IleVal: 2.734 ± 1.176
0.684IleTrp: 0.684 ± 0.444
2.734IleTyr: 2.734 ± 0.661
0.0IleXaa: 0.0 ± 0.0
Lys
7.177LysAla: 7.177 ± 1.303
0.0LysCys: 0.0 ± 0.0
3.076LysAsp: 3.076 ± 1.108
6.494LysGlu: 6.494 ± 1.431
5.126LysPhe: 5.126 ± 1.231
5.126LysGly: 5.126 ± 1.161
1.709LysHis: 1.709 ± 0.601
6.835LysIle: 6.835 ± 1.124
9.228LysLys: 9.228 ± 1.781
9.911LysLeu: 9.911 ± 2.086
4.101LysMet: 4.101 ± 1.466
8.202LysAsn: 8.202 ± 2.164
1.025LysPro: 1.025 ± 0.425
3.759LysGln: 3.759 ± 1.1
5.81LysArg: 5.81 ± 1.118
10.595LysSer: 10.595 ± 1.254
5.81LysThr: 5.81 ± 1.128
6.494LysVal: 6.494 ± 1.33
1.709LysTrp: 1.709 ± 0.548
2.734LysTyr: 2.734 ± 0.947
0.0LysXaa: 0.0 ± 0.0
Leu
5.468LeuAla: 5.468 ± 1.258
0.342LeuCys: 0.342 ± 0.301
4.785LeuAsp: 4.785 ± 1.239
7.861LeuGlu: 7.861 ± 1.513
5.126LeuPhe: 5.126 ± 1.049
4.101LeuGly: 4.101 ± 1.016
3.759LeuHis: 3.759 ± 1.824
5.126LeuIle: 5.126 ± 1.11
8.544LeuLys: 8.544 ± 1.393
7.177LeuLeu: 7.177 ± 0.9
3.076LeuMet: 3.076 ± 0.946
5.126LeuAsn: 5.126 ± 1.035
2.392LeuPro: 2.392 ± 0.637
2.392LeuGln: 2.392 ± 0.747
5.126LeuArg: 5.126 ± 0.881
8.202LeuSer: 8.202 ± 1.594
3.076LeuThr: 3.076 ± 0.828
6.152LeuVal: 6.152 ± 1.355
0.342LeuTrp: 0.342 ± 0.359
3.076LeuTyr: 3.076 ± 0.636
0.0LeuXaa: 0.0 ± 0.0
Met
1.709MetAla: 1.709 ± 0.601
0.0MetCys: 0.0 ± 0.0
2.734MetAsp: 2.734 ± 0.79
0.684MetGlu: 0.684 ± 0.565
0.342MetPhe: 0.342 ± 0.307
1.025MetGly: 1.025 ± 0.61
0.0MetHis: 0.0 ± 0.0
0.684MetIle: 0.684 ± 0.565
4.785MetLys: 4.785 ± 1.199
1.709MetLeu: 1.709 ± 0.804
0.0MetMet: 0.0 ± 0.0
2.392MetAsn: 2.392 ± 0.923
0.342MetPro: 0.342 ± 0.393
1.025MetGln: 1.025 ± 0.656
1.367MetArg: 1.367 ± 0.68
2.734MetSer: 2.734 ± 0.776
2.051MetThr: 2.051 ± 0.77
1.367MetVal: 1.367 ± 0.699
0.0MetTrp: 0.0 ± 0.0
1.025MetTyr: 1.025 ± 0.514
0.0MetXaa: 0.0 ± 0.0
Asn
4.101AsnAla: 4.101 ± 0.883
0.342AsnCys: 0.342 ± 0.313
2.392AsnAsp: 2.392 ± 1.072
4.443AsnGlu: 4.443 ± 1.312
4.101AsnPhe: 4.101 ± 1.398
3.076AsnGly: 3.076 ± 0.708
1.025AsnHis: 1.025 ± 1.096
5.126AsnIle: 5.126 ± 1.289
6.835AsnLys: 6.835 ± 1.333
5.126AsnLeu: 5.126 ± 1.397
0.342AsnMet: 0.342 ± 0.292
5.468AsnAsn: 5.468 ± 1.46
2.392AsnPro: 2.392 ± 0.803
2.051AsnGln: 2.051 ± 0.849
2.051AsnArg: 2.051 ± 0.699
3.076AsnSer: 3.076 ± 1.192
2.734AsnThr: 2.734 ± 0.609
1.025AsnVal: 1.025 ± 0.52
0.684AsnTrp: 0.684 ± 0.439
2.734AsnTyr: 2.734 ± 0.744
0.0AsnXaa: 0.0 ± 0.0
Pro
1.025ProAla: 1.025 ± 0.659
0.342ProCys: 0.342 ± 0.301
1.367ProAsp: 1.367 ± 0.789
2.734ProGlu: 2.734 ± 0.922
0.684ProPhe: 0.684 ± 0.495
0.0ProGly: 0.0 ± 0.0
0.0ProHis: 0.0 ± 0.0
2.051ProIle: 2.051 ± 0.751
2.392ProLys: 2.392 ± 0.725
2.392ProLeu: 2.392 ± 0.613
0.342ProMet: 0.342 ± 0.325
0.684ProAsn: 0.684 ± 0.423
0.684ProPro: 0.684 ± 0.503
1.367ProGln: 1.367 ± 0.6
1.367ProArg: 1.367 ± 0.59
0.684ProSer: 0.684 ± 0.392
1.367ProThr: 1.367 ± 0.761
0.684ProVal: 0.684 ± 0.418
0.684ProTrp: 0.684 ± 0.392
1.367ProTyr: 1.367 ± 0.676
0.0ProXaa: 0.0 ± 0.0
Gln
3.076GlnAla: 3.076 ± 1.199
0.0GlnCys: 0.0 ± 0.0
1.025GlnAsp: 1.025 ± 0.461
2.051GlnGlu: 2.051 ± 0.843
0.684GlnPhe: 0.684 ± 0.36
0.0GlnGly: 0.0 ± 0.0
1.025GlnHis: 1.025 ± 0.444
3.418GlnIle: 3.418 ± 0.841
3.759GlnLys: 3.759 ± 0.76
3.759GlnLeu: 3.759 ± 0.852
2.051GlnMet: 2.051 ± 0.835
2.392GlnAsn: 2.392 ± 0.9
0.342GlnPro: 0.342 ± 0.313
2.051GlnGln: 2.051 ± 1.077
2.734GlnArg: 2.734 ± 0.856
2.734GlnSer: 2.734 ± 1.206
1.025GlnThr: 1.025 ± 0.521
2.051GlnVal: 2.051 ± 0.892
0.342GlnTrp: 0.342 ± 0.301
0.684GlnTyr: 0.684 ± 0.516
0.0GlnXaa: 0.0 ± 0.0
Arg
2.051ArgAla: 2.051 ± 0.785
0.342ArgCys: 0.342 ± 0.373
3.076ArgAsp: 3.076 ± 1.083
4.443ArgGlu: 4.443 ± 1.023
2.051ArgPhe: 2.051 ± 0.786
3.418ArgGly: 3.418 ± 0.618
2.051ArgHis: 2.051 ± 0.785
4.785ArgIle: 4.785 ± 1.395
6.152ArgLys: 6.152 ± 1.897
4.101ArgLeu: 4.101 ± 1.17
0.342ArgMet: 0.342 ± 0.314
1.709ArgAsn: 1.709 ± 1.021
0.684ArgPro: 0.684 ± 0.626
1.367ArgGln: 1.367 ± 0.685
1.367ArgArg: 1.367 ± 0.794
2.392ArgSer: 2.392 ± 0.616
3.759ArgThr: 3.759 ± 0.917
2.051ArgVal: 2.051 ± 0.933
0.684ArgTrp: 0.684 ± 0.437
1.709ArgTyr: 1.709 ± 0.734
0.0ArgXaa: 0.0 ± 0.0
Ser
2.734SerAla: 2.734 ± 0.725
0.0SerCys: 0.0 ± 0.0
3.759SerAsp: 3.759 ± 0.936
4.785SerGlu: 4.785 ± 1.609
4.101SerPhe: 4.101 ± 0.899
2.051SerGly: 2.051 ± 0.656
1.709SerHis: 1.709 ± 0.825
7.861SerIle: 7.861 ± 2.54
6.494SerLys: 6.494 ± 1.147
6.494SerLeu: 6.494 ± 1.561
1.025SerMet: 1.025 ± 0.588
5.81SerAsn: 5.81 ± 0.954
1.709SerPro: 1.709 ± 0.721
1.367SerGln: 1.367 ± 0.618
3.759SerArg: 3.759 ± 1.081
4.443SerSer: 4.443 ± 1.304
5.468SerThr: 5.468 ± 1.29
4.785SerVal: 4.785 ± 0.945
1.025SerTrp: 1.025 ± 0.638
2.734SerTyr: 2.734 ± 1.106
0.0SerXaa: 0.0 ± 0.0
Thr
2.734ThrAla: 2.734 ± 0.759
0.684ThrCys: 0.684 ± 0.417
3.418ThrAsp: 3.418 ± 0.922
4.443ThrGlu: 4.443 ± 1.42
3.418ThrPhe: 3.418 ± 1.52
3.759ThrGly: 3.759 ± 1.072
0.684ThrHis: 0.684 ± 0.478
3.418ThrIle: 3.418 ± 1.472
5.468ThrLys: 5.468 ± 1.246
5.126ThrLeu: 5.126 ± 1.11
1.367ThrMet: 1.367 ± 0.703
0.684ThrAsn: 0.684 ± 0.43
1.025ThrPro: 1.025 ± 0.444
0.684ThrGln: 0.684 ± 0.476
2.392ThrArg: 2.392 ± 0.982
3.418ThrSer: 3.418 ± 1.271
3.076ThrThr: 3.076 ± 1.18
3.759ThrVal: 3.759 ± 1.29
0.342ThrTrp: 0.342 ± 0.325
1.709ThrTyr: 1.709 ± 0.704
0.0ThrXaa: 0.0 ± 0.0
Val
3.418ValAla: 3.418 ± 0.916
0.0ValCys: 0.0 ± 0.0
2.392ValAsp: 2.392 ± 0.851
4.443ValGlu: 4.443 ± 0.988
2.734ValPhe: 2.734 ± 1.145
0.684ValGly: 0.684 ± 0.475
0.684ValHis: 0.684 ± 0.483
6.835ValIle: 6.835 ± 1.432
5.126ValLys: 5.126 ± 1.058
3.418ValLeu: 3.418 ± 0.839
1.709ValMet: 1.709 ± 0.842
2.392ValAsn: 2.392 ± 1.255
0.684ValPro: 0.684 ± 0.4
2.051ValGln: 2.051 ± 1.065
2.734ValArg: 2.734 ± 1.2
4.443ValSer: 4.443 ± 0.954
3.418ValThr: 3.418 ± 1.267
4.101ValVal: 4.101 ± 1.217
0.342ValTrp: 0.342 ± 0.389
2.051ValTyr: 2.051 ± 1.125
0.0ValXaa: 0.0 ± 0.0
Trp
0.342TrpAla: 0.342 ± 0.313
0.0TrpCys: 0.0 ± 0.0
0.0TrpAsp: 0.0 ± 0.0
1.709TrpGlu: 1.709 ± 0.959
0.342TrpPhe: 0.342 ± 0.324
0.342TrpGly: 0.342 ± 0.301
0.342TrpHis: 0.342 ± 0.365
1.025TrpIle: 1.025 ± 0.403
0.342TrpLys: 0.342 ± 0.324
0.684TrpLeu: 0.684 ± 0.517
0.0TrpMet: 0.0 ± 0.0
0.342TrpAsn: 0.342 ± 0.365
0.0TrpPro: 0.0 ± 0.0
1.025TrpGln: 1.025 ± 0.545
0.684TrpArg: 0.684 ± 0.423
1.367TrpSer: 1.367 ± 0.704
0.342TrpThr: 0.342 ± 0.324
0.684TrpVal: 0.684 ± 0.479
0.342TrpTrp: 0.342 ± 0.313
1.025TrpTyr: 1.025 ± 0.515
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.709TyrAla: 1.709 ± 0.656
0.342TyrCys: 0.342 ± 0.373
2.051TyrAsp: 2.051 ± 0.806
2.734TyrGlu: 2.734 ± 0.886
2.734TyrPhe: 2.734 ± 0.629
2.392TyrGly: 2.392 ± 1.085
1.367TyrHis: 1.367 ± 0.541
2.051TyrIle: 2.051 ± 0.862
4.443TyrLys: 4.443 ± 1.545
4.101TyrLeu: 4.101 ± 1.225
0.684TyrMet: 0.684 ± 0.745
2.392TyrAsn: 2.392 ± 0.962
1.709TyrPro: 1.709 ± 0.538
2.392TyrGln: 2.392 ± 0.926
1.025TyrArg: 1.025 ± 0.488
2.051TyrSer: 2.051 ± 1.018
1.367TyrThr: 1.367 ± 0.469
1.025TyrVal: 1.025 ± 0.515
0.342TyrTrp: 0.342 ± 0.313
1.709TyrTyr: 1.709 ± 0.686
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 21 proteins (2927 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski