Rinderpest morbillivirus
Average proteome isoelectric point is 6.69
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0H5BN48|A0A0H5BN48_9MONO RNA-directed RNA polymerase L OS=Rinderpest morbillivirus OX=11241 GN=L PE=3 SV=1
MM1 pKa = 7.87 ASLLKK6 pKa = 10.78 SLALFKK12 pKa = 10.79 KK13 pKa = 10.55 NKK15 pKa = 9.77 DD16 pKa = 3.72 KK17 pKa = 11.19 PPLAAGSGGAIRR29 pKa = 11.84 GIKK32 pKa = 9.67 HH33 pKa = 4.91 VVIVPIPGDD42 pKa = 3.45 SSITTRR48 pKa = 11.84 SRR50 pKa = 11.84 LLDD53 pKa = 3.8 CLVKK57 pKa = 10.26 MVGDD61 pKa = 4.29 PDD63 pKa = 3.28 ISGPKK68 pKa = 8.56 LTGALISILSLFVEE82 pKa = 4.97 SPGQLIQRR90 pKa = 11.84 ITDD93 pKa = 3.95 DD94 pKa = 3.86 PDD96 pKa = 3.35 ISIKK100 pKa = 10.5 LVEE103 pKa = 4.51 VVQSDD108 pKa = 3.6 KK109 pKa = 10.51 TQSGLTFASRR119 pKa = 11.84 GASMDD124 pKa = 4.08 DD125 pKa = 3.13 EE126 pKa = 5.21 ADD128 pKa = 3.54 RR129 pKa = 11.84 YY130 pKa = 8.44 FTYY133 pKa = 10.53 DD134 pKa = 3.23 EE135 pKa = 4.83 PNGGEE140 pKa = 3.99 EE141 pKa = 3.93 RR142 pKa = 11.84 QSYY145 pKa = 7.97 WFEE148 pKa = 3.59 NRR150 pKa = 11.84 EE151 pKa = 3.84 IQDD154 pKa = 3.55 IEE156 pKa = 4.3 VQDD159 pKa = 3.84 PEE161 pKa = 4.65 GFNMILATILAQIWILLAKK180 pKa = 10.35 AVTTPDD186 pKa = 3.1 TAADD190 pKa = 3.58 SEE192 pKa = 4.07 LRR194 pKa = 11.84 RR195 pKa = 11.84 WVKK198 pKa = 8.71 YY199 pKa = 5.31 TQQRR203 pKa = 11.84 RR204 pKa = 11.84 VIGEE208 pKa = 3.69 FRR210 pKa = 11.84 LDD212 pKa = 3.82 KK213 pKa = 11.05 GWLDD217 pKa = 3.56 TVRR220 pKa = 11.84 NRR222 pKa = 11.84 IAEE225 pKa = 4.26 DD226 pKa = 3.22 LSLRR230 pKa = 11.84 RR231 pKa = 11.84 FMVALILDD239 pKa = 4.4 IKK241 pKa = 9.43 RR242 pKa = 11.84 TPGNKK247 pKa = 8.91 PRR249 pKa = 11.84 IAEE252 pKa = 4.48 MICDD256 pKa = 3.34 IDD258 pKa = 3.96 TYY260 pKa = 10.84 IVEE263 pKa = 4.69 AGLASFILTIKK274 pKa = 10.64 FGIEE278 pKa = 3.56 TMYY281 pKa = 10.26 PALGLHH287 pKa = 6.29 EE288 pKa = 4.47 FAGEE292 pKa = 4.11 LSTIEE297 pKa = 4.35 SLMNLYY303 pKa = 9.91 QQMGEE308 pKa = 4.06 LAPYY312 pKa = 9.25 MVILEE317 pKa = 4.19 NSIQNKK323 pKa = 9.08 FSAGAYY329 pKa = 7.48 PLLWSYY335 pKa = 12.09 AMGVGVEE342 pKa = 4.94 LEE344 pKa = 4.14 SSMGGLNFGRR354 pKa = 11.84 SYY356 pKa = 11.19 FDD358 pKa = 3.04 PAYY361 pKa = 10.4 FRR363 pKa = 11.84 LGQEE367 pKa = 3.88 MVRR370 pKa = 11.84 RR371 pKa = 11.84 SAGKK375 pKa = 9.88 VSSNLASEE383 pKa = 4.65 LGITEE388 pKa = 4.25 EE389 pKa = 4.02 EE390 pKa = 3.97 AKK392 pKa = 10.57 LVSEE396 pKa = 4.48 IAAYY400 pKa = 9.24 TGDD403 pKa = 3.9 DD404 pKa = 3.6 RR405 pKa = 11.84 NSRR408 pKa = 11.84 TSGPKK413 pKa = 7.44 QTQVSFLRR421 pKa = 11.84 TDD423 pKa = 3.05 QGGEE427 pKa = 3.85 IQHH430 pKa = 6.17 NASKK434 pKa = 10.52 KK435 pKa = 10.04 DD436 pKa = 3.57 EE437 pKa = 4.14 ARR439 pKa = 11.84 VPQVRR444 pKa = 11.84 KK445 pKa = 7.92 EE446 pKa = 4.05 TWASSRR452 pKa = 11.84 LDD454 pKa = 3.4 RR455 pKa = 11.84 YY456 pKa = 10.79 KK457 pKa = 11.05 EE458 pKa = 3.97 DD459 pKa = 3.65 TDD461 pKa = 3.91 NEE463 pKa = 4.3 AVSPSVKK470 pKa = 9.93 TLIDD474 pKa = 3.24 VDD476 pKa = 4.11 TTPEE480 pKa = 4.29 GDD482 pKa = 3.59 TDD484 pKa = 4.05 PLGSKK489 pKa = 10.16 KK490 pKa = 10.31 SAEE493 pKa = 4.69 AILKK497 pKa = 7.94 LQAMASILGDD507 pKa = 3.46 STLGNDD513 pKa = 4.77 SPRR516 pKa = 11.84 TYY518 pKa = 10.98 NDD520 pKa = 3.51 KK521 pKa = 11.24 DD522 pKa = 3.73 LLSS525 pKa = 3.86
Molecular weight: 57.99 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.972
IPC2_protein 4.94
IPC_protein 4.889
Toseland 4.736
ProMoST 4.991
Dawson 4.838
Bjellqvist 4.978
Wikipedia 4.711
Rodwell 4.736
Grimsley 4.647
Solomon 4.838
Lehninger 4.787
Nozaki 4.94
DTASelect 5.118
Thurlkill 4.736
EMBOSS 4.724
Sillero 5.003
Patrickios 4.342
IPC_peptide 4.838
IPC2_peptide 4.991
IPC2.peptide.svr19 5.025
Protein with the highest isoelectric point:
>tr|A0A0H5B5J8|A0A0H5B5J8_9MONO Hemagglutinin glycoprotein OS=Rinderpest morbillivirus OX=11241 GN=H PE=3 SV=1
MM1 pKa = 8.04 AEE3 pKa = 4.59 IYY5 pKa = 10.89 DD6 pKa = 3.97 FDD8 pKa = 4.53 KK9 pKa = 11.39 SSWDD13 pKa = 3.5 VKK15 pKa = 11.09 GSIAPIRR22 pKa = 11.84 PTTYY26 pKa = 10.35 NDD28 pKa = 2.86 GRR30 pKa = 11.84 LIPQVRR36 pKa = 11.84 VIDD39 pKa = 4.23 PGLGDD44 pKa = 4.23 RR45 pKa = 11.84 KK46 pKa = 10.47 DD47 pKa = 3.46 EE48 pKa = 4.05 CFMYY52 pKa = 10.31 IFLLGIVEE60 pKa = 4.84 DD61 pKa = 4.71 SDD63 pKa = 4.01 PLSPPRR69 pKa = 11.84 GRR71 pKa = 11.84 TFGSLPLGVGKK82 pKa = 8.51 STAKK86 pKa = 10.45 PEE88 pKa = 4.09 EE89 pKa = 4.1 LLKK92 pKa = 10.8 EE93 pKa = 4.18 ATDD96 pKa = 3.72 LDD98 pKa = 3.63 IVVRR102 pKa = 11.84 RR103 pKa = 11.84 TAGLNEE109 pKa = 3.91 KK110 pKa = 10.39 LVFYY114 pKa = 11.06 NNTPLSLLTPWKK126 pKa = 10.42 KK127 pKa = 10.27 ILTKK131 pKa = 10.83 GSVFSANQVCSAVNLIPLDD150 pKa = 3.51 TPQRR154 pKa = 11.84 FRR156 pKa = 11.84 VVYY159 pKa = 9.03 MSITRR164 pKa = 11.84 LSDD167 pKa = 3.2 SGCYY171 pKa = 8.94 NVPRR175 pKa = 11.84 KK176 pKa = 8.81 MLEE179 pKa = 3.85 FRR181 pKa = 11.84 SANAMAFNLLVTLKK195 pKa = 9.77 IDD197 pKa = 3.35 TGTEE201 pKa = 3.6 PGRR204 pKa = 11.84 PEE206 pKa = 3.8 VGAVGLSEE214 pKa = 4.15 ATFMVHH220 pKa = 6.08 IGNFRR225 pKa = 11.84 RR226 pKa = 11.84 KK227 pKa = 9.89 KK228 pKa = 9.91 NEE230 pKa = 3.86 AYY232 pKa = 10.46 SADD235 pKa = 3.68 YY236 pKa = 11.12 CKK238 pKa = 10.39 MKK240 pKa = 10.01 IEE242 pKa = 4.25 KK243 pKa = 9.11 MGLVFALGGIGGTSLHH259 pKa = 6.26 IRR261 pKa = 11.84 STGKK265 pKa = 9.26 MSKK268 pKa = 8.35 TLHH271 pKa = 5.95 AQLGFKK277 pKa = 9.06 KK278 pKa = 8.43 TLCYY282 pKa = 10.37 PLMDD286 pKa = 4.35 INEE289 pKa = 4.46 DD290 pKa = 3.64 LNRR293 pKa = 11.84 LLWRR297 pKa = 11.84 SKK299 pKa = 10.71 CKK301 pKa = 9.75 IVRR304 pKa = 11.84 IQAVIQPSVPQEE316 pKa = 3.3 FRR318 pKa = 11.84 IYY320 pKa = 11.01 DD321 pKa = 3.56 DD322 pKa = 5.15 VIINDD327 pKa = 3.69 DD328 pKa = 3.35 QGLFKK333 pKa = 11.11 VLL335 pKa = 3.52
Molecular weight: 37.46 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.552
IPC2_protein 8.419
IPC_protein 8.317
Toseland 8.931
ProMoST 8.843
Dawson 9.268
Bjellqvist 9.165
Wikipedia 9.399
Rodwell 9.472
Grimsley 9.238
Solomon 9.355
Lehninger 9.326
Nozaki 9.341
DTASelect 9.048
Thurlkill 9.18
EMBOSS 9.428
Sillero 9.355
Patrickios 4.838
IPC_peptide 9.341
IPC2_peptide 7.863
IPC2.peptide.svr19 7.707
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
4705
335
2183
784.2
87.34
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.1 ± 0.497
1.679 ± 0.225
5.845 ± 0.487
5.292 ± 0.329
3.103 ± 0.487
6.652 ± 0.724
2.232 ± 0.51
7.736 ± 0.219
5.547 ± 0.435
10.797 ± 0.633
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.147 ± 0.148
3.953 ± 0.264
4.612 ± 0.465
3.337 ± 0.195
5.59 ± 0.453
8.714 ± 0.526
6.015 ± 0.247
6.036 ± 0.226
1.02 ± 0.09
3.592 ± 0.496
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here