Dyella tabacisoli
Average proteome isoelectric point is 6.66
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4066 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A369UKQ3|A0A369UKQ3_9GAMM MoxR family ATPase OS=Dyella tabacisoli OX=2282381 GN=DVJ77_12515 PE=4 SV=1
MM1 pKa = 7.07 SQSSTEE7 pKa = 4.05 TKK9 pKa = 10.62 ALSKK13 pKa = 9.67 WMCVVCGFIYY23 pKa = 10.65 DD24 pKa = 3.97 EE25 pKa = 5.19 AEE27 pKa = 4.0 GLPDD31 pKa = 3.62 EE32 pKa = 5.37 GIEE35 pKa = 4.8 PGTRR39 pKa = 11.84 WADD42 pKa = 3.34 VPAAWTCPDD51 pKa = 3.81 CGATKK56 pKa = 10.7 DD57 pKa = 3.88 DD58 pKa = 4.33 FEE60 pKa = 5.12 MIEE63 pKa = 3.83 IDD65 pKa = 3.43
Molecular weight: 7.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.769
IPC2_protein 3.834
IPC_protein 3.732
Toseland 3.541
ProMoST 3.808
Dawson 3.719
Bjellqvist 3.948
Wikipedia 3.656
Rodwell 3.567
Grimsley 3.465
Solomon 3.694
Lehninger 3.643
Nozaki 3.859
DTASelect 4.024
Thurlkill 3.605
EMBOSS 3.668
Sillero 3.846
Patrickios 1.863
IPC_peptide 3.694
IPC2_peptide 3.821
IPC2.peptide.svr19 3.832
Protein with the highest isoelectric point:
>tr|A0A369ULK8|A0A369ULK8_9GAMM MFS transporter OS=Dyella tabacisoli OX=2282381 GN=DVJ77_10760 PE=4 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.01 RR4 pKa = 11.84 TFQPSKK10 pKa = 10.06 LKK12 pKa = 10.38 RR13 pKa = 11.84 ARR15 pKa = 11.84 THH17 pKa = 5.89 GFRR20 pKa = 11.84 ARR22 pKa = 11.84 MATADD27 pKa = 3.2 GRR29 pKa = 11.84 KK30 pKa = 9.07 ILNARR35 pKa = 11.84 RR36 pKa = 11.84 AKK38 pKa = 9.77 GRR40 pKa = 11.84 KK41 pKa = 8.92 RR42 pKa = 11.84 LIPP45 pKa = 4.02
Molecular weight: 5.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.403
IPC2_protein 10.862
IPC_protein 12.442
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.34
Grimsley 12.647
Solomon 13.1
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.076
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.067
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4066
0
4066
1484847
22
13240
365.2
39.72
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.013 ± 0.054
0.83 ± 0.012
5.622 ± 0.028
4.82 ± 0.054
3.407 ± 0.026
8.268 ± 0.058
2.467 ± 0.021
4.534 ± 0.024
2.992 ± 0.04
10.859 ± 0.069
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.182 ± 0.021
3.078 ± 0.05
5.214 ± 0.032
4.157 ± 0.029
6.635 ± 0.056
6.034 ± 0.046
5.502 ± 0.063
7.185 ± 0.036
1.51 ± 0.021
2.69 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here