Chlamydia virus CPAR39
Average proteome isoelectric point is 7.07
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q9MBM5|Q9MBM5_9VIRU Uncharacterized protein OS=Chlamydia virus CPAR39 OX=1986029 GN=CPA0005 PE=4 SV=1
MM1 pKa = 7.92 DD2 pKa = 5.32 ASLMQFLKK10 pKa = 10.7 KK11 pKa = 10.9 VFDD14 pKa = 4.34 FYY16 pKa = 11.48 VAEE19 pKa = 4.07 VCIFFVPFLTSFF31 pKa = 3.63
Molecular weight: 3.68 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.743
IPC2_protein 5.118
IPC_protein 4.393
Toseland 4.228
ProMoST 4.584
Dawson 4.393
Bjellqvist 4.558
Wikipedia 4.368
Rodwell 4.24
Grimsley 4.164
Solomon 4.355
Lehninger 4.304
Nozaki 4.558
DTASelect 4.711
Thurlkill 4.329
EMBOSS 4.368
Sillero 4.52
Patrickios 3.719
IPC_peptide 4.355
IPC2_peptide 4.495
IPC2.peptide.svr19 4.508
Protein with the highest isoelectric point:
>tr|Q9MBM8|Q9MBM8_9VIRU Uncharacterized protein OS=Chlamydia virus CPAR39 OX=1986029 GN=CPA0001 PE=3 SV=1
MM1 pKa = 7.42 NPEE4 pKa = 4.11 QLTSTLGSAVSGVAQGLSFLPGIASGVLGYY34 pKa = 10.88 LGAQKK39 pKa = 10.61 QNATAKK45 pKa = 10.27 QIARR49 pKa = 11.84 EE50 pKa = 3.76 QMAFQEE56 pKa = 4.44 RR57 pKa = 11.84 MSNTAYY63 pKa = 9.83 QRR65 pKa = 11.84 AMEE68 pKa = 4.44 DD69 pKa = 3.17 MKK71 pKa = 11.23 KK72 pKa = 10.66 AGLNPMLAFSKK83 pKa = 10.84 GGASSPAGASWSPNNPVEE101 pKa = 4.26 SAMNSGLAVQRR112 pKa = 11.84 LTYY115 pKa = 9.8 EE116 pKa = 4.07 RR117 pKa = 11.84 KK118 pKa = 9.96 KK119 pKa = 9.85 MQAEE123 pKa = 4.06 LQNLRR128 pKa = 11.84 EE129 pKa = 4.1 QNRR132 pKa = 11.84 LIRR135 pKa = 11.84 NQAIRR140 pKa = 11.84 EE141 pKa = 4.47 GYY143 pKa = 9.02 LAEE146 pKa = 4.21 RR147 pKa = 11.84 DD148 pKa = 3.19 KK149 pKa = 11.42 YY150 pKa = 10.07 MRR152 pKa = 11.84 VAGVPVATEE161 pKa = 3.79 MLDD164 pKa = 3.44 RR165 pKa = 11.84 TSGLLSSSAKK175 pKa = 10.16 AFKK178 pKa = 10.81 NLFSRR183 pKa = 11.84 KK184 pKa = 8.8 GRR186 pKa = 3.67
Molecular weight: 20.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.235
IPC2_protein 9.56
IPC_protein 9.867
Toseland 10.423
ProMoST 10.145
Dawson 10.555
Bjellqvist 10.204
Wikipedia 10.716
Rodwell 10.965
Grimsley 10.599
Solomon 10.599
Lehninger 10.57
Nozaki 10.379
DTASelect 10.204
Thurlkill 10.423
EMBOSS 10.804
Sillero 10.467
Patrickios 10.672
IPC_peptide 10.599
IPC2_peptide 8.741
IPC2.peptide.svr19 8.763
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
1280
31
553
213.3
24.35
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.641 ± 1.411
1.875 ± 0.611
4.844 ± 0.671
6.016 ± 1.142
5.469 ± 0.639
6.172 ± 0.948
1.328 ± 0.315
3.75 ± 0.81
6.563 ± 2.08
8.047 ± 0.517
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.813 ± 0.749
4.766 ± 0.395
6.094 ± 0.577
5.078 ± 0.377
6.406 ± 0.66
7.344 ± 0.661
4.922 ± 1.28
5.781 ± 0.373
1.406 ± 0.409
4.688 ± 0.808
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here