Lactococcus phage ASCC284

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Skunavirus; unclassified Skunavirus

Average proteome isoelectric point is 6.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 57 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|H9EDH7|H9EDH7_9CAUD Uncharacterized protein OS=Lactococcus phage ASCC284 OX=1165137 GN=LLAPH_284_0003 PE=4 SV=1
MM1 pKa = 6.72KK2 pKa = 9.92QYY4 pKa = 10.25WVIEE8 pKa = 4.07SHH10 pKa = 7.07LDD12 pKa = 3.01GGLYY16 pKa = 10.5VMPEE20 pKa = 3.91DD21 pKa = 4.02TPEE24 pKa = 3.9EE25 pKa = 3.99EE26 pKa = 4.03LRR28 pKa = 11.84EE29 pKa = 4.04VEE31 pKa = 5.18VYY33 pKa = 10.89CDD35 pKa = 3.26TCGDD39 pKa = 3.72SDD41 pKa = 4.28SVIGLFSNWEE51 pKa = 3.87QLKK54 pKa = 10.64KK55 pKa = 11.07EE56 pKa = 4.12MTDD59 pKa = 3.64DD60 pKa = 4.7EE61 pKa = 4.81DD62 pKa = 3.4WCPYY66 pKa = 9.63SDD68 pKa = 5.57EE69 pKa = 4.19YY70 pKa = 11.25LQSLFEE76 pKa = 4.15

Molecular weight: 9.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|H9EDI7|H9EDI7_9CAUD Neck passage structure protein OS=Lactococcus phage ASCC284 OX=1165137 GN=LLAPH_284_0013 PE=4 SV=1
MM1 pKa = 7.64AVNLLNTSSIAKK13 pKa = 9.58EE14 pKa = 3.88MQTKK18 pKa = 7.47VTEE21 pKa = 4.24RR22 pKa = 11.84MGDD25 pKa = 2.9WFEE28 pKa = 5.92AEE30 pKa = 4.62FKK32 pKa = 11.01VKK34 pKa = 10.6ANSASRR40 pKa = 11.84RR41 pKa = 11.84TRR43 pKa = 11.84LIRR46 pKa = 11.84SHH48 pKa = 5.25GHH50 pKa = 5.22TYY52 pKa = 9.85TYY54 pKa = 11.35ARR56 pKa = 11.84YY57 pKa = 9.69QNTGQLSSNLKK68 pKa = 8.86QVKK71 pKa = 10.12KK72 pKa = 10.27GDD74 pKa = 3.72KK75 pKa = 9.93VVVNAGTRR83 pKa = 11.84ANYY86 pKa = 8.51TSGYY90 pKa = 8.75HH91 pKa = 6.14GMYY94 pKa = 10.51FLVEE98 pKa = 4.16KK99 pKa = 10.74KK100 pKa = 10.71GMQDD104 pKa = 3.28VKK106 pKa = 9.28TTLKK110 pKa = 10.68KK111 pKa = 10.0GANYY115 pKa = 9.69ANSMKK120 pKa = 10.65LL121 pKa = 3.26

Molecular weight: 13.68 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

57

0

57

9679

39

996

169.8

19.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.941 ± 0.569

0.796 ± 0.212

5.682 ± 0.306

7.707 ± 0.704

4.639 ± 0.284

6.53 ± 0.652

1.219 ± 0.157

6.891 ± 0.241

8.73 ± 0.463

8.503 ± 0.42

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.418 ± 0.142

6.778 ± 0.316

2.283 ± 0.236

3.513 ± 0.208

3.151 ± 0.242

6.468 ± 0.499

6.478 ± 0.363

6.478 ± 0.368

1.519 ± 0.133

4.277 ± 0.4
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski