Amino acid dipepetide frequency for Lactococcus phage ASCC284

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
0.62AlaAla: 0.62 ± 0.282
0.207AlaCys: 0.207 ± 0.17
3.41AlaAsp: 3.41 ± 0.634
4.443AlaGlu: 4.443 ± 0.756
3.72AlaPhe: 3.72 ± 0.78
4.34AlaGly: 4.34 ± 0.828
0.93AlaHis: 0.93 ± 0.348
5.373AlaIle: 5.373 ± 0.832
5.27AlaLys: 5.27 ± 0.851
5.786AlaLeu: 5.786 ± 0.59
1.757AlaMet: 1.757 ± 0.579
4.443AlaAsn: 4.443 ± 0.76
1.033AlaPro: 1.033 ± 0.327
2.17AlaGln: 2.17 ± 0.504
2.17AlaArg: 2.17 ± 0.384
2.583AlaSer: 2.583 ± 0.637
3.513AlaThr: 3.513 ± 0.888
4.34AlaVal: 4.34 ± 1.206
1.653AlaTrp: 1.653 ± 0.652
1.653AlaTyr: 1.653 ± 0.456
0.0AlaXaa: 0.0 ± 0.0
Cys
0.31CysAla: 0.31 ± 0.182
0.413CysCys: 0.413 ± 0.289
0.62CysAsp: 0.62 ± 0.318
0.413CysGlu: 0.413 ± 0.219
0.31CysPhe: 0.31 ± 0.164
0.827CysGly: 0.827 ± 0.376
0.207CysHis: 0.207 ± 0.182
0.723CysIle: 0.723 ± 0.277
0.62CysLys: 0.62 ± 0.329
0.31CysLeu: 0.31 ± 0.166
0.0CysMet: 0.0 ± 0.0
0.517CysAsn: 0.517 ± 0.251
0.207CysPro: 0.207 ± 0.161
0.207CysGln: 0.207 ± 0.122
0.62CysArg: 0.62 ± 0.236
0.207CysSer: 0.207 ± 0.168
0.207CysThr: 0.207 ± 0.131
0.517CysVal: 0.517 ± 0.285
0.103CysTrp: 0.103 ± 0.095
0.62CysTyr: 0.62 ± 0.327
0.0CysXaa: 0.0 ± 0.0
Asp
1.55AspAla: 1.55 ± 0.345
0.413AspCys: 0.413 ± 0.208
3.41AspAsp: 3.41 ± 0.772
4.856AspGlu: 4.856 ± 0.921
3.72AspPhe: 3.72 ± 0.516
3.1AspGly: 3.1 ± 0.581
0.413AspHis: 0.413 ± 0.303
3.306AspIle: 3.306 ± 0.596
5.58AspLys: 5.58 ± 0.639
5.786AspLeu: 5.786 ± 0.699
1.033AspMet: 1.033 ± 0.326
4.133AspAsn: 4.133 ± 0.673
1.343AspPro: 1.343 ± 0.441
0.827AspGln: 0.827 ± 0.358
1.86AspArg: 1.86 ± 0.524
3.306AspSer: 3.306 ± 0.6
4.856AspThr: 4.856 ± 0.746
3.1AspVal: 3.1 ± 0.752
1.137AspTrp: 1.137 ± 0.391
3.1AspTyr: 3.1 ± 0.466
0.0AspXaa: 0.0 ± 0.0
Glu
3.72GluAla: 3.72 ± 0.581
0.723GluCys: 0.723 ± 0.325
3.203GluAsp: 3.203 ± 0.661
5.89GluGlu: 5.89 ± 1.025
3.823GluPhe: 3.823 ± 0.658
2.996GluGly: 2.996 ± 0.525
1.033GluHis: 1.033 ± 0.309
5.89GluIle: 5.89 ± 0.7
7.13GluLys: 7.13 ± 1.435
10.436GluLeu: 10.436 ± 1.911
2.893GluMet: 2.893 ± 0.659
4.96GluAsn: 4.96 ± 0.597
1.447GluPro: 1.447 ± 0.377
3.203GluGln: 3.203 ± 0.714
2.893GluArg: 2.893 ± 0.626
3.616GluSer: 3.616 ± 0.504
4.546GluThr: 4.546 ± 0.683
4.546GluVal: 4.546 ± 0.564
1.24GluTrp: 1.24 ± 0.408
2.893GluTyr: 2.893 ± 0.618
0.0GluXaa: 0.0 ± 0.0
Phe
3.823PheAla: 3.823 ± 0.655
0.31PheCys: 0.31 ± 0.19
3.513PheAsp: 3.513 ± 0.651
2.79PheGlu: 2.79 ± 0.539
2.17PhePhe: 2.17 ± 0.778
2.48PheGly: 2.48 ± 0.461
0.31PheHis: 0.31 ± 0.145
3.306PheIle: 3.306 ± 0.502
4.443PheLys: 4.443 ± 0.667
2.893PheLeu: 2.893 ± 0.52
0.723PheMet: 0.723 ± 0.279
3.823PheAsn: 3.823 ± 0.807
1.033PhePro: 1.033 ± 0.34
1.55PheGln: 1.55 ± 0.455
1.033PheArg: 1.033 ± 0.252
4.236PheSer: 4.236 ± 0.96
2.893PheThr: 2.893 ± 0.485
2.48PheVal: 2.48 ± 0.461
0.31PheTrp: 0.31 ± 0.153
2.273PheTyr: 2.273 ± 0.449
0.0PheXaa: 0.0 ± 0.0
Gly
3.306GlyAla: 3.306 ± 0.814
0.413GlyCys: 0.413 ± 0.203
3.513GlyAsp: 3.513 ± 0.676
3.823GlyGlu: 3.823 ± 0.614
2.377GlyPhe: 2.377 ± 0.546
5.27GlyGly: 5.27 ± 0.967
0.62GlyHis: 0.62 ± 0.23
4.856GlyIle: 4.856 ± 1.669
5.476GlyLys: 5.476 ± 0.675
6.2GlyLeu: 6.2 ± 1.097
1.343GlyMet: 1.343 ± 0.464
3.926GlyAsn: 3.926 ± 0.665
0.207GlyPro: 0.207 ± 0.148
2.48GlyGln: 2.48 ± 0.513
1.86GlyArg: 1.86 ± 0.334
5.063GlySer: 5.063 ± 0.972
3.616GlyThr: 3.616 ± 0.881
5.993GlyVal: 5.993 ± 1.192
1.447GlyTrp: 1.447 ± 0.321
3.513GlyTyr: 3.513 ± 0.651
0.0GlyXaa: 0.0 ± 0.0
His
0.62HisAla: 0.62 ± 0.289
0.62HisCys: 0.62 ± 0.283
0.62HisAsp: 0.62 ± 0.232
0.827HisGlu: 0.827 ± 0.303
0.517HisPhe: 0.517 ± 0.248
1.033HisGly: 1.033 ± 0.42
0.103HisHis: 0.103 ± 0.108
0.827HisIle: 0.827 ± 0.277
0.62HisLys: 0.62 ± 0.255
1.343HisLeu: 1.343 ± 0.484
0.0HisMet: 0.0 ± 0.0
1.757HisAsn: 1.757 ± 0.554
0.103HisPro: 0.103 ± 0.084
0.413HisGln: 0.413 ± 0.196
0.103HisArg: 0.103 ± 0.099
0.517HisSer: 0.517 ± 0.205
0.827HisThr: 0.827 ± 0.346
0.827HisVal: 0.827 ± 0.334
0.103HisTrp: 0.103 ± 0.096
0.413HisTyr: 0.413 ± 0.206
0.0HisXaa: 0.0 ± 0.0
Ile
4.133IleAla: 4.133 ± 0.808
0.31IleCys: 0.31 ± 0.183
4.133IleAsp: 4.133 ± 0.745
5.993IleGlu: 5.993 ± 0.889
3.203IlePhe: 3.203 ± 0.775
4.133IleGly: 4.133 ± 0.841
1.033IleHis: 1.033 ± 0.33
4.96IleIle: 4.96 ± 0.592
6.096IleLys: 6.096 ± 0.744
4.236IleLeu: 4.236 ± 0.883
1.447IleMet: 1.447 ± 0.319
5.476IleAsn: 5.476 ± 0.667
1.757IlePro: 1.757 ± 0.428
2.377IleGln: 2.377 ± 0.454
1.757IleArg: 1.757 ± 0.465
4.03IleSer: 4.03 ± 0.8
5.27IleThr: 5.27 ± 0.843
4.856IleVal: 4.856 ± 0.767
1.137IleTrp: 1.137 ± 0.376
2.583IleTyr: 2.583 ± 0.497
0.0IleXaa: 0.0 ± 0.0
Lys
6.716LysAla: 6.716 ± 0.98
0.413LysCys: 0.413 ± 0.266
4.236LysAsp: 4.236 ± 0.543
8.576LysGlu: 8.576 ± 1.373
2.583LysPhe: 2.583 ± 0.546
5.063LysGly: 5.063 ± 0.763
1.55LysHis: 1.55 ± 0.444
5.166LysIle: 5.166 ± 0.729
8.473LysLys: 8.473 ± 1.137
7.44LysLeu: 7.44 ± 0.931
3.616LysMet: 3.616 ± 0.603
4.546LysAsn: 4.546 ± 0.64
1.447LysPro: 1.447 ± 0.367
3.1LysGln: 3.1 ± 0.743
3.1LysArg: 3.1 ± 0.698
5.166LysSer: 5.166 ± 0.742
4.96LysThr: 4.96 ± 0.681
6.613LysVal: 6.613 ± 1.019
1.033LysTrp: 1.033 ± 0.28
3.41LysTyr: 3.41 ± 0.764
0.0LysXaa: 0.0 ± 0.0
Leu
5.166LeuAla: 5.166 ± 0.616
0.103LeuCys: 0.103 ± 0.138
5.27LeuAsp: 5.27 ± 0.764
6.2LeuGlu: 6.2 ± 0.878
4.34LeuPhe: 4.34 ± 0.629
4.753LeuGly: 4.753 ± 0.805
1.137LeuHis: 1.137 ± 0.437
6.303LeuIle: 6.303 ± 0.843
8.06LeuLys: 8.06 ± 0.969
5.89LeuLeu: 5.89 ± 1.1
1.55LeuMet: 1.55 ± 0.472
5.58LeuAsn: 5.58 ± 0.792
2.996LeuPro: 2.996 ± 0.516
2.996LeuGln: 2.996 ± 0.488
2.687LeuArg: 2.687 ± 0.511
4.96LeuSer: 4.96 ± 0.798
5.58LeuThr: 5.58 ± 0.749
5.476LeuVal: 5.476 ± 0.674
1.447LeuTrp: 1.447 ± 0.394
4.546LeuTyr: 4.546 ± 0.754
0.0LeuXaa: 0.0 ± 0.0
Met
2.687MetAla: 2.687 ± 0.603
0.207MetCys: 0.207 ± 0.166
1.447MetAsp: 1.447 ± 0.411
1.757MetGlu: 1.757 ± 0.568
0.31MetPhe: 0.31 ± 0.221
0.93MetGly: 0.93 ± 0.297
0.207MetHis: 0.207 ± 0.148
2.273MetIle: 2.273 ± 0.459
3.1MetLys: 3.1 ± 0.656
1.137MetLeu: 1.137 ± 0.364
0.413MetMet: 0.413 ± 0.223
1.653MetAsn: 1.653 ± 0.437
0.62MetPro: 0.62 ± 0.271
1.343MetGln: 1.343 ± 0.379
0.413MetArg: 0.413 ± 0.237
1.55MetSer: 1.55 ± 0.459
1.757MetThr: 1.757 ± 0.432
1.137MetVal: 1.137 ± 0.229
0.0MetTrp: 0.0 ± 0.0
1.24MetTyr: 1.24 ± 0.345
0.0MetXaa: 0.0 ± 0.0
Asn
5.476AsnAla: 5.476 ± 1.219
0.103AsnCys: 0.103 ± 0.098
4.03AsnAsp: 4.03 ± 0.599
4.96AsnGlu: 4.96 ± 0.84
2.48AsnPhe: 2.48 ± 0.627
6.613AsnGly: 6.613 ± 0.725
0.827AsnHis: 0.827 ± 0.27
4.236AsnIle: 4.236 ± 0.687
6.096AsnLys: 6.096 ± 0.992
5.476AsnLeu: 5.476 ± 0.749
1.447AsnMet: 1.447 ± 0.313
3.1AsnAsn: 3.1 ± 0.643
2.17AsnPro: 2.17 ± 0.449
2.067AsnGln: 2.067 ± 0.413
1.757AsnArg: 1.757 ± 0.382
5.373AsnSer: 5.373 ± 0.922
4.133AsnThr: 4.133 ± 0.577
3.513AsnVal: 3.513 ± 0.566
1.343AsnTrp: 1.343 ± 0.342
2.583AsnTyr: 2.583 ± 0.644
0.0AsnXaa: 0.0 ± 0.0
Pro
1.137ProAla: 1.137 ± 0.354
0.207ProCys: 0.207 ± 0.149
1.757ProAsp: 1.757 ± 0.561
2.48ProGlu: 2.48 ± 0.589
1.137ProPhe: 1.137 ± 0.316
0.827ProGly: 0.827 ± 0.524
0.103ProHis: 0.103 ± 0.113
1.447ProIle: 1.447 ± 0.357
1.55ProLys: 1.55 ± 0.404
1.86ProLeu: 1.86 ± 0.378
0.723ProMet: 0.723 ± 0.243
2.17ProAsn: 2.17 ± 0.592
0.723ProPro: 0.723 ± 0.283
0.62ProGln: 0.62 ± 0.279
0.31ProArg: 0.31 ± 0.144
1.343ProSer: 1.343 ± 0.401
2.067ProThr: 2.067 ± 0.39
1.757ProVal: 1.757 ± 0.482
0.103ProTrp: 0.103 ± 0.084
0.517ProTyr: 0.517 ± 0.238
0.0ProXaa: 0.0 ± 0.0
Gln
3.203GlnAla: 3.203 ± 0.7
0.103GlnCys: 0.103 ± 0.132
1.757GlnAsp: 1.757 ± 0.533
2.48GlnGlu: 2.48 ± 0.532
0.93GlnPhe: 0.93 ± 0.336
3.203GlnGly: 3.203 ± 0.605
0.413GlnHis: 0.413 ± 0.197
1.137GlnIle: 1.137 ± 0.256
2.79GlnLys: 2.79 ± 0.63
2.996GlnLeu: 2.996 ± 0.606
0.827GlnMet: 0.827 ± 0.243
2.273GlnAsn: 2.273 ± 0.385
1.137GlnPro: 1.137 ± 0.339
1.447GlnGln: 1.447 ± 0.41
1.963GlnArg: 1.963 ± 0.521
2.273GlnSer: 2.273 ± 0.372
1.963GlnThr: 1.963 ± 0.316
2.17GlnVal: 2.17 ± 0.491
0.62GlnTrp: 0.62 ± 0.214
1.447GlnTyr: 1.447 ± 0.395
0.0GlnXaa: 0.0 ± 0.0
Arg
2.48ArgAla: 2.48 ± 0.659
0.31ArgCys: 0.31 ± 0.2
1.343ArgAsp: 1.343 ± 0.398
2.48ArgGlu: 2.48 ± 0.563
0.723ArgPhe: 0.723 ± 0.256
1.86ArgGly: 1.86 ± 0.436
0.517ArgHis: 0.517 ± 0.231
2.067ArgIle: 2.067 ± 0.477
3.203ArgLys: 3.203 ± 0.626
3.203ArgLeu: 3.203 ± 0.641
0.517ArgMet: 0.517 ± 0.308
2.583ArgAsn: 2.583 ± 0.489
0.31ArgPro: 0.31 ± 0.192
1.343ArgGln: 1.343 ± 0.337
1.24ArgArg: 1.24 ± 0.374
1.447ArgSer: 1.447 ± 0.374
2.17ArgThr: 2.17 ± 0.393
1.86ArgVal: 1.86 ± 0.453
0.517ArgTrp: 0.517 ± 0.211
1.343ArgTyr: 1.343 ± 0.441
0.0ArgXaa: 0.0 ± 0.0
Ser
4.443SerAla: 4.443 ± 1.01
0.723SerCys: 0.723 ± 0.329
3.926SerAsp: 3.926 ± 0.515
4.133SerGlu: 4.133 ± 0.636
3.513SerPhe: 3.513 ± 0.766
5.683SerGly: 5.683 ± 1.284
0.62SerHis: 0.62 ± 0.273
4.133SerIle: 4.133 ± 0.75
4.236SerLys: 4.236 ± 0.611
5.58SerLeu: 5.58 ± 0.679
2.17SerMet: 2.17 ± 0.381
3.926SerAsn: 3.926 ± 0.746
1.137SerPro: 1.137 ± 0.361
2.377SerGln: 2.377 ± 0.553
2.17SerArg: 2.17 ± 0.394
5.993SerSer: 5.993 ± 1.064
2.79SerThr: 2.79 ± 0.561
3.823SerVal: 3.823 ± 0.681
0.93SerTrp: 0.93 ± 0.344
2.377SerTyr: 2.377 ± 0.514
0.0SerXaa: 0.0 ± 0.0
Thr
4.34ThrAla: 4.34 ± 0.726
0.517ThrCys: 0.517 ± 0.267
3.926ThrAsp: 3.926 ± 0.764
5.373ThrGlu: 5.373 ± 0.758
3.513ThrPhe: 3.513 ± 0.686
5.27ThrGly: 5.27 ± 0.784
0.413ThrHis: 0.413 ± 0.178
4.443ThrIle: 4.443 ± 0.8
4.856ThrLys: 4.856 ± 0.571
5.476ThrLeu: 5.476 ± 0.732
0.827ThrMet: 0.827 ± 0.293
4.34ThrAsn: 4.34 ± 0.788
2.377ThrPro: 2.377 ± 0.478
2.067ThrGln: 2.067 ± 0.447
1.137ThrArg: 1.137 ± 0.388
3.926ThrSer: 3.926 ± 0.614
4.65ThrThr: 4.65 ± 0.847
4.753ThrVal: 4.753 ± 0.922
0.723ThrTrp: 0.723 ± 0.354
1.86ThrTyr: 1.86 ± 0.442
0.0ThrXaa: 0.0 ± 0.0
Val
3.41ValAla: 3.41 ± 0.582
0.517ValCys: 0.517 ± 0.236
3.926ValAsp: 3.926 ± 0.755
4.65ValGlu: 4.65 ± 0.651
3.306ValPhe: 3.306 ± 0.809
3.41ValGly: 3.41 ± 0.631
0.413ValHis: 0.413 ± 0.189
4.856ValIle: 4.856 ± 0.651
6.406ValLys: 6.406 ± 0.786
3.513ValLeu: 3.513 ± 0.507
1.86ValMet: 1.86 ± 0.469
3.306ValAsn: 3.306 ± 0.698
1.653ValPro: 1.653 ± 0.492
1.757ValGln: 1.757 ± 0.451
2.893ValArg: 2.893 ± 0.765
5.786ValSer: 5.786 ± 1.288
5.373ValThr: 5.373 ± 0.938
3.616ValVal: 3.616 ± 0.834
0.723ValTrp: 0.723 ± 0.219
3.41ValTyr: 3.41 ± 0.563
0.0ValXaa: 0.0 ± 0.0
Trp
0.62TrpAla: 0.62 ± 0.176
0.517TrpCys: 0.517 ± 0.266
0.827TrpAsp: 0.827 ± 0.3
1.033TrpGlu: 1.033 ± 0.343
1.137TrpPhe: 1.137 ± 0.435
0.723TrpGly: 0.723 ± 0.291
0.207TrpHis: 0.207 ± 0.168
0.723TrpIle: 0.723 ± 0.286
0.93TrpLys: 0.93 ± 0.298
1.447TrpLeu: 1.447 ± 0.585
0.31TrpMet: 0.31 ± 0.211
1.447TrpAsn: 1.447 ± 0.544
0.0TrpPro: 0.0 ± 0.0
0.93TrpGln: 0.93 ± 0.245
0.517TrpArg: 0.517 ± 0.263
1.24TrpSer: 1.24 ± 0.318
0.517TrpThr: 0.517 ± 0.241
0.93TrpVal: 0.93 ± 0.33
0.207TrpTrp: 0.207 ± 0.152
0.93TrpTyr: 0.93 ± 0.276
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.653TyrAla: 1.653 ± 0.481
0.827TyrCys: 0.827 ± 0.338
1.963TyrAsp: 1.963 ± 0.633
3.926TyrGlu: 3.926 ± 0.727
2.583TyrPhe: 2.583 ± 0.497
2.79TyrGly: 2.79 ± 0.505
1.137TyrHis: 1.137 ± 0.374
2.893TyrIle: 2.893 ± 0.73
2.377TyrLys: 2.377 ± 0.525
4.03TyrLeu: 4.03 ± 0.714
0.62TyrMet: 0.62 ± 0.295
3.823TyrAsn: 3.823 ± 0.44
1.137TyrPro: 1.137 ± 0.386
1.86TyrGln: 1.86 ± 0.511
1.033TyrArg: 1.033 ± 0.334
2.067TyrSer: 2.067 ± 0.455
3.1TyrThr: 3.1 ± 0.742
2.48TyrVal: 2.48 ± 0.48
0.413TyrTrp: 0.413 ± 0.186
2.067TyrTyr: 2.067 ± 0.545
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 57 proteins (9679 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski