Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL) (Black stem rust fungus)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Pucciniomycotina; Pucciniomycetes; Pucciniales; Pucciniaceae; Puccinia; Puccinia graminis; Puccinia graminis f. sp. tritici

Average proteome isoelectric point is 7.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 15808 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E3LAP3|E3LAP3_PUCGT Uncharacterized protein OS=Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL) OX=418459 GN=PGTG_19617 PE=4 SV=2
MM1 pKa = 7.58PRR3 pKa = 11.84FSPNEE8 pKa = 4.01DD9 pKa = 3.25SSSDD13 pKa = 3.59DD14 pKa = 4.35FYY16 pKa = 11.75SSSNASPKK24 pKa = 10.46SPSISSKK31 pKa = 10.0ATGDD35 pKa = 3.4SDD37 pKa = 4.63EE38 pKa = 5.05DD39 pKa = 4.27HH40 pKa = 7.3SDD42 pKa = 4.43DD43 pKa = 5.27PNTSDD48 pKa = 5.34DD49 pKa = 4.32QPDD52 pKa = 3.76TNIFNCNLYY61 pKa = 10.88GSTTGSSSCSNSNDD75 pKa = 3.81SNEE78 pKa = 4.16HH79 pKa = 6.62RR80 pKa = 11.84SDD82 pKa = 5.18ADD84 pKa = 4.8DD85 pKa = 4.05KK86 pKa = 11.94DD87 pKa = 4.23EE88 pKa = 4.76NNSCSEE94 pKa = 3.95EE95 pKa = 3.92DD96 pKa = 3.45SYY98 pKa = 11.82LISSSYY104 pKa = 9.56NHH106 pKa = 6.77CKK108 pKa = 9.83PSNSSEE114 pKa = 3.92EE115 pKa = 4.38SEE117 pKa = 4.8DD118 pKa = 4.05GSDD121 pKa = 3.56ATDD124 pKa = 3.12SDD126 pKa = 4.58KK127 pKa = 11.75EE128 pKa = 4.21HH129 pKa = 6.88VDD131 pKa = 3.66YY132 pKa = 11.29SASNN136 pKa = 3.28

Molecular weight: 14.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|H6QSI2|H6QSI2_PUCGT AGC protein kinase OS=Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL) OX=418459 GN=PGTG_21795 PE=4 SV=1
MM1 pKa = 8.38VMGTGRR7 pKa = 11.84WRR9 pKa = 11.84KK10 pKa = 9.59VMPQQDD16 pKa = 3.6RR17 pKa = 11.84QSISLQLTAIRR28 pKa = 11.84TRR30 pKa = 11.84MKK32 pKa = 9.67VTGRR36 pKa = 11.84LPDD39 pKa = 3.64PNMRR43 pKa = 11.84MRR45 pKa = 11.84SRR47 pKa = 11.84SRR49 pKa = 11.84VRR51 pKa = 11.84RR52 pKa = 11.84PISIRR57 pKa = 11.84AGFSNN62 pKa = 3.64

Molecular weight: 7.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

15688

120

15808

6249486

29

5214

395.3

43.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.125 ± 0.018

1.326 ± 0.008

5.413 ± 0.015

5.885 ± 0.02

3.603 ± 0.012

5.8 ± 0.019

2.865 ± 0.011

5.157 ± 0.012

5.539 ± 0.018

9.348 ± 0.02

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.95 ± 0.008

4.494 ± 0.012

6.677 ± 0.021

4.722 ± 0.015

5.689 ± 0.017

9.69 ± 0.026

5.976 ± 0.014

5.141 ± 0.013

1.23 ± 0.006

2.37 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski