Amino acid dipepetide frequency for Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL) (Black stem rust fungus)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.035AlaAla: 6.035 ± 0.042	0.982AlaCys: 0.982 ± 0.013	3.549AlaAsp: 3.549 ± 0.027	4.137AlaGlu: 4.137 ± 0.033	2.441AlaPhe: 2.441 ± 0.022	4.46AlaGly: 4.46 ± 0.034	1.923AlaHis: 1.923 ± 0.018	3.83AlaIle: 3.83 ± 0.03	3.862AlaLys: 3.862 ± 0.031	6.418AlaLeu: 6.418 ± 0.042	1.464AlaMet: 1.464 ± 0.017	3.018AlaAsn: 3.018 ± 0.025	4.361AlaPro: 4.361 ± 0.037	3.221AlaGln: 3.221 ± 0.026	4.142AlaArg: 4.142 ± 0.029	6.807AlaSer: 6.807 ± 0.04	4.379AlaThr: 4.379 ± 0.028	3.724AlaVal: 3.724 ± 0.024	0.844AlaTrp: 0.844 ± 0.014	1.648AlaTyr: 1.648 ± 0.016	0.0AlaXaa: 0.0 ± 0.0
Cys	0.81CysAla: 0.81 ± 0.012	0.295CysCys: 0.295 ± 0.007	0.689CysAsp: 0.689 ± 0.011	0.615CysGlu: 0.615 ± 0.01	0.583CysPhe: 0.583 ± 0.011	0.938CysGly: 0.938 ± 0.012	0.386CysHis: 0.386 ± 0.008	0.61CysIle: 0.61 ± 0.009	0.667CysLys: 0.667 ± 0.011	1.366CysLeu: 1.366 ± 0.015	0.272CysMet: 0.272 ± 0.007	0.499CysAsn: 0.499 ± 0.009	0.87CysPro: 0.87 ± 0.014	0.641CysGln: 0.641 ± 0.012	0.818CysArg: 0.818 ± 0.012	1.225CysSer: 1.225 ± 0.015	0.721CysThr: 0.721 ± 0.012	0.655CysVal: 0.655 ± 0.011	0.205CysTrp: 0.205 ± 0.006	0.398CysTyr: 0.398 ± 0.008	0.0CysXaa: 0.0 ± 0.0
Asp	3.124AspAla: 3.124 ± 0.021	0.736AspCys: 0.736 ± 0.01	3.904AspAsp: 3.904 ± 0.038	4.164AspGlu: 4.164 ± 0.033	2.057AspPhe: 2.057 ± 0.018	3.415AspGly: 3.415 ± 0.025	1.804AspHis: 1.804 ± 0.019	2.377AspIle: 2.377 ± 0.019	2.311AspLys: 2.311 ± 0.024	5.405AspLeu: 5.405 ± 0.033	0.959AspMet: 0.959 ± 0.013	1.988AspAsn: 1.988 ± 0.018	3.935AspPro: 3.935 ± 0.028	2.951AspGln: 2.951 ± 0.026	3.108AspArg: 3.108 ± 0.027	4.691AspSer: 4.691 ± 0.036	2.347AspThr: 2.347 ± 0.022	2.637AspVal: 2.637 ± 0.025	0.822AspTrp: 0.822 ± 0.011	1.391AspTyr: 1.391 ± 0.015	0.0AspXaa: 0.0 ± 0.0
Glu	4.512GluAla: 4.512 ± 0.032	0.61GluCys: 0.61 ± 0.01	4.002GluAsp: 4.002 ± 0.032	5.612GluGlu: 5.612 ± 0.053	1.975GluPhe: 1.975 ± 0.02	3.051GluGly: 3.051 ± 0.025	1.338GluHis: 1.338 ± 0.016	3.289GluIle: 3.289 ± 0.029	3.599GluLys: 3.599 ± 0.034	5.591GluLeu: 5.591 ± 0.035	1.272GluMet: 1.272 ± 0.014	2.482GluAsn: 2.482 ± 0.024	2.856GluPro: 2.856 ± 0.025	2.468GluGln: 2.468 ± 0.022	3.319GluArg: 3.319 ± 0.031	4.598GluSer: 4.598 ± 0.033	3.103GluThr: 3.103 ± 0.025	3.095GluVal: 3.095 ± 0.024	0.72GluTrp: 0.72 ± 0.011	1.36GluTyr: 1.36 ± 0.014	0.0GluXaa: 0.0 ± 0.0
Phe	2.203PheAla: 2.203 ± 0.022	0.6PheCys: 0.6 ± 0.01	2.204PheAsp: 2.204 ± 0.022	2.092PheGlu: 2.092 ± 0.019	1.594PhePhe: 1.594 ± 0.017	2.463PheGly: 2.463 ± 0.026	1.024PheHis: 1.024 ± 0.014	1.86PheIle: 1.86 ± 0.02	1.992PheLys: 1.992 ± 0.019	3.514PheLeu: 3.514 ± 0.029	0.701PheMet: 0.701 ± 0.012	1.72PheAsn: 1.72 ± 0.016	1.899PhePro: 1.899 ± 0.018	1.647PheGln: 1.647 ± 0.017	1.907PheArg: 1.907 ± 0.021	3.185PheSer: 3.185 ± 0.026	1.896PheThr: 1.896 ± 0.017	2.033PheVal: 2.033 ± 0.02	0.522PheTrp: 0.522 ± 0.011	0.978PheTyr: 0.978 ± 0.013	0.0PheXaa: 0.0 ± 0.0
Gly	3.743GlyAla: 3.743 ± 0.034	0.888GlyCys: 0.888 ± 0.012	2.679GlyAsp: 2.679 ± 0.025	2.796GlyGlu: 2.796 ± 0.026	2.524GlyPhe: 2.524 ± 0.025	4.693GlyGly: 4.693 ± 0.068	1.576GlyHis: 1.576 ± 0.019	3.077GlyIle: 3.077 ± 0.025	3.203GlyLys: 3.203 ± 0.026	5.595GlyLeu: 5.595 ± 0.04	1.187GlyMet: 1.187 ± 0.017	2.385GlyAsn: 2.385 ± 0.023	3.29GlyPro: 3.29 ± 0.028	2.441GlyGln: 2.441 ± 0.025	3.391GlyArg: 3.391 ± 0.03	5.563GlySer: 5.563 ± 0.045	3.333GlyThr: 3.333 ± 0.03	3.086GlyVal: 3.086 ± 0.026	0.874GlyTrp: 0.874 ± 0.012	1.682GlyTyr: 1.682 ± 0.021	0.0GlyXaa: 0.0 ± 0.0
His	1.564HisAla: 1.564 ± 0.019	0.42HisCys: 0.42 ± 0.008	1.292HisAsp: 1.292 ± 0.015	1.386HisGlu: 1.386 ± 0.014	1.06HisPhe: 1.06 ± 0.013	1.414HisGly: 1.414 ± 0.015	1.783HisHis: 1.783 ± 0.028	1.229HisIle: 1.229 ± 0.014	1.149HisLys: 1.149 ± 0.015	2.97HisLeu: 2.97 ± 0.024	0.487HisMet: 0.487 ± 0.01	1.098HisAsn: 1.098 ± 0.014	2.566HisPro: 2.566 ± 0.025	2.01HisGln: 2.01 ± 0.024	1.736HisArg: 1.736 ± 0.016	2.739HisSer: 2.739 ± 0.028	1.385HisThr: 1.385 ± 0.014	1.286HisVal: 1.286 ± 0.014	0.35HisTrp: 0.35 ± 0.007	0.726HisTyr: 0.726 ± 0.009	0.0HisXaa: 0.0 ± 0.0
Ile	3.331IleAla: 3.331 ± 0.028	0.865IleCys: 0.865 ± 0.013	3.172IleAsp: 3.172 ± 0.022	3.162IleGlu: 3.162 ± 0.028	1.943IlePhe: 1.943 ± 0.018	2.995IleGly: 2.995 ± 0.026	1.494IleHis: 1.494 ± 0.018	2.684IleIle: 2.684 ± 0.027	2.997IleLys: 2.997 ± 0.024	4.779IleLeu: 4.779 ± 0.031	0.924IleMet: 0.924 ± 0.011	2.441IleAsn: 2.441 ± 0.019	3.375IlePro: 3.375 ± 0.026	2.447IleGln: 2.447 ± 0.021	2.973IleArg: 2.973 ± 0.021	4.566IleSer: 4.566 ± 0.03	2.789IleThr: 2.789 ± 0.022	2.705IleVal: 2.705 ± 0.022	0.638IleTrp: 0.638 ± 0.009	1.289IleTyr: 1.289 ± 0.016	0.0IleXaa: 0.0 ± 0.0
Lys	4.249LysAla: 4.249 ± 0.031	0.61LysCys: 0.61 ± 0.01	2.8LysAsp: 2.8 ± 0.022	3.505LysGlu: 3.505 ± 0.03	1.881LysPhe: 1.881 ± 0.022	2.576LysGly: 2.576 ± 0.023	1.305LysHis: 1.305 ± 0.014	3.01LysIle: 3.01 ± 0.023	4.581LysLys: 4.581 ± 0.045	5.277LysLeu: 5.277 ± 0.032	1.147LysMet: 1.147 ± 0.016	2.512LysAsn: 2.512 ± 0.023	3.45LysPro: 3.45 ± 0.031	2.311LysGln: 2.311 ± 0.023	3.485LysArg: 3.485 ± 0.026	4.647LysSer: 4.647 ± 0.031	3.429LysThr: 3.429 ± 0.03	2.674LysVal: 2.674 ± 0.024	0.646LysTrp: 0.646 ± 0.012	1.295LysTyr: 1.295 ± 0.017	0.0LysXaa: 0.0 ± 0.0
Leu	7.295LeuAla: 7.295 ± 0.036	1.233LeuCys: 1.233 ± 0.014	5.508LeuAsp: 5.508 ± 0.03	5.675LeuGlu: 5.675 ± 0.038	3.305LeuPhe: 3.305 ± 0.027	5.428LeuGly: 5.428 ± 0.036	2.361LeuHis: 2.361 ± 0.02	5.016LeuIle: 5.016 ± 0.035	5.571LeuLys: 5.571 ± 0.04	8.742LeuLeu: 8.742 ± 0.055	1.908LeuMet: 1.908 ± 0.021	4.22LeuAsn: 4.22 ± 0.031	6.031LeuPro: 6.031 ± 0.041	3.945LeuGln: 3.945 ± 0.029	5.126LeuArg: 5.126 ± 0.03	8.492LeuSer: 8.492 ± 0.044	5.195LeuThr: 5.195 ± 0.031	5.269LeuVal: 5.269 ± 0.036	1.057LeuTrp: 1.057 ± 0.016	2.101LeuTyr: 2.101 ± 0.02	0.0LeuXaa: 0.0 ± 0.0
Met	1.753MetAla: 1.753 ± 0.019	0.24MetCys: 0.24 ± 0.006	1.143MetAsp: 1.143 ± 0.016	1.16MetGlu: 1.16 ± 0.015	0.688MetPhe: 0.688 ± 0.01	1.149MetGly: 1.149 ± 0.015	0.38MetHis: 0.38 ± 0.008	1.199MetIle: 1.199 ± 0.014	1.195MetLys: 1.195 ± 0.015	1.573MetLeu: 1.573 ± 0.017	0.559MetMet: 0.559 ± 0.011	0.99MetAsn: 0.99 ± 0.013	0.96MetPro: 0.96 ± 0.014	0.659MetGln: 0.659 ± 0.012	1.023MetArg: 1.023 ± 0.013	1.791MetSer: 1.791 ± 0.016	1.188MetThr: 1.188 ± 0.011	1.192MetVal: 1.192 ± 0.013	0.216MetTrp: 0.216 ± 0.006	0.446MetTyr: 0.446 ± 0.007	0.0MetXaa: 0.0 ± 0.0
Asn	2.553AsnAla: 2.553 ± 0.022	0.627AsnCys: 0.627 ± 0.01	2.11AsnAsp: 2.11 ± 0.015	2.321AsnGlu: 2.321 ± 0.019	1.609AsnPhe: 1.609 ± 0.017	2.617AsnGly: 2.617 ± 0.027	1.691AsnHis: 1.691 ± 0.019	2.003AsnIle: 2.003 ± 0.017	2.217AsnLys: 2.217 ± 0.02	4.402AsnLeu: 4.402 ± 0.027	0.763AsnMet: 0.763 ± 0.012	2.586AsnAsn: 2.586 ± 0.038	3.711AsnPro: 3.711 ± 0.026	2.805AsnGln: 2.805 ± 0.022	2.557AsnArg: 2.557 ± 0.021	4.214AsnSer: 4.214 ± 0.033	2.441AsnThr: 2.441 ± 0.021	1.984AsnVal: 1.984 ± 0.018	0.567AsnTrp: 0.567 ± 0.01	1.156AsnTyr: 1.156 ± 0.015	0.0AsnXaa: 0.0 ± 0.0
Pro	5.239ProAla: 5.239 ± 0.044	0.601ProCys: 0.601 ± 0.011	3.279ProAsp: 3.279 ± 0.026	3.597ProGlu: 3.597 ± 0.032	1.994ProPhe: 1.994 ± 0.017	3.576ProGly: 3.576 ± 0.037	1.808ProHis: 1.808 ± 0.02	3.355ProIle: 3.355 ± 0.023	3.334ProLys: 3.334 ± 0.03	5.178ProLeu: 5.178 ± 0.038	1.091ProMet: 1.091 ± 0.014	3.122ProAsn: 3.122 ± 0.028	6.241ProPro: 6.241 ± 0.062	2.824ProGln: 2.824 ± 0.027	3.217ProArg: 3.217 ± 0.026	8.14ProSer: 8.14 ± 0.057	4.955ProThr: 4.955 ± 0.035	3.454ProVal: 3.454 ± 0.027	0.569ProTrp: 0.569 ± 0.01	1.192ProTyr: 1.192 ± 0.015	0.0ProXaa: 0.0 ± 0.0
Gln	3.884GlnAla: 3.884 ± 0.031	0.479GlnCys: 0.479 ± 0.009	2.311GlnAsp: 2.311 ± 0.021	2.771GlnGlu: 2.771 ± 0.025	1.467GlnPhe: 1.467 ± 0.017	2.061GlnGly: 2.061 ± 0.023	1.4GlnHis: 1.4 ± 0.017	2.461GlnIle: 2.461 ± 0.021	2.467GlnLys: 2.467 ± 0.025	4.681GlnLeu: 4.681 ± 0.038	0.919GlnMet: 0.919 ± 0.012	1.997GlnAsn: 1.997 ± 0.019	3.44GlnPro: 3.44 ± 0.032	3.352GlnGln: 3.352 ± 0.058	2.415GlnArg: 2.415 ± 0.021	4.394GlnSer: 4.394 ± 0.03	2.853GlnThr: 2.853 ± 0.022	2.414GlnVal: 2.414 ± 0.022	0.525GlnTrp: 0.525 ± 0.01	0.928GlnTyr: 0.928 ± 0.013	0.0GlnXaa: 0.0 ± 0.0
Arg	4.061ArgAla: 4.061 ± 0.029	0.762ArgCys: 0.762 ± 0.011	2.601ArgAsp: 2.601 ± 0.023	2.971ArgGlu: 2.971 ± 0.03	2.147ArgPhe: 2.147 ± 0.017	2.944ArgGly: 2.944 ± 0.028	1.518ArgHis: 1.518 ± 0.018	2.964ArgIle: 2.964 ± 0.021	3.516ArgLys: 3.516 ± 0.027	5.836ArgLeu: 5.836 ± 0.036	1.205ArgMet: 1.205 ± 0.014	2.315ArgAsn: 2.315 ± 0.021	3.678ArgPro: 3.678 ± 0.03	2.554ArgGln: 2.554 ± 0.023	4.302ArgArg: 4.302 ± 0.034	5.289ArgSer: 5.289 ± 0.039	3.185ArgThr: 3.185 ± 0.025	2.83ArgVal: 2.83 ± 0.022	0.82ArgTrp: 0.82 ± 0.012	1.391ArgTyr: 1.391 ± 0.016	0.0ArgXaa: 0.0 ± 0.0
Ser	6.616SerAla: 6.616 ± 0.038	1.136SerCys: 1.136 ± 0.016	4.832SerAsp: 4.832 ± 0.029	4.631SerGlu: 4.631 ± 0.031	3.36SerPhe: 3.36 ± 0.024	5.307SerGly: 5.307 ± 0.039	2.736SerHis: 2.736 ± 0.025	4.802SerIle: 4.802 ± 0.034	4.974SerLys: 4.974 ± 0.029	8.37SerLeu: 8.37 ± 0.046	1.752SerMet: 1.752 ± 0.016	4.738SerAsn: 4.738 ± 0.038	6.453SerPro: 6.453 ± 0.058	4.451SerGln: 4.451 ± 0.036	5.223SerArg: 5.223 ± 0.036	13.239SerSer: 13.239 ± 0.107	6.91SerThr: 6.91 ± 0.049	4.413SerVal: 4.413 ± 0.029	0.987SerTrp: 0.987 ± 0.016	1.973SerTyr: 1.973 ± 0.019	0.0SerXaa: 0.0 ± 0.0
Thr	4.157ThrAla: 4.157 ± 0.03	0.827ThrCys: 0.827 ± 0.013	2.792ThrAsp: 2.792 ± 0.022	2.971ThrGlu: 2.971 ± 0.023	1.997ThrPhe: 1.997 ± 0.019	3.581ThrGly: 3.581 ± 0.03	1.656ThrHis: 1.656 ± 0.015	3.18ThrIle: 3.18 ± 0.021	2.971ThrLys: 2.971 ± 0.026	5.27ThrLeu: 5.27 ± 0.033	1.062ThrMet: 1.062 ± 0.012	2.837ThrAsn: 2.837 ± 0.028	4.562ThrPro: 4.562 ± 0.03	2.596ThrGln: 2.596 ± 0.021	3.331ThrArg: 3.331 ± 0.023	6.143ThrSer: 6.143 ± 0.046	4.886ThrThr: 4.886 ± 0.047	2.935ThrVal: 2.935 ± 0.026	0.702ThrTrp: 0.702 ± 0.011	1.303ThrTyr: 1.303 ± 0.016	0.0ThrXaa: 0.0 ± 0.0
Val	3.831ValAla: 3.831 ± 0.029	0.783ValCys: 0.783 ± 0.012	3.241ValAsp: 3.241 ± 0.023	3.327ValGlu: 3.327 ± 0.022	1.954ValPhe: 1.954 ± 0.019	3.144ValGly: 3.144 ± 0.029	1.358ValHis: 1.358 ± 0.015	2.761ValIle: 2.761 ± 0.024	2.799ValLys: 2.799 ± 0.024	4.737ValLeu: 4.737 ± 0.028	1.047ValMet: 1.047 ± 0.015	2.177ValAsn: 2.177 ± 0.019	3.083ValPro: 3.083 ± 0.025	2.186ValGln: 2.186 ± 0.023	2.676ValArg: 2.676 ± 0.022	4.162ValSer: 4.162 ± 0.029	2.84ValThr: 2.84 ± 0.024	3.233ValVal: 3.233 ± 0.028	0.706ValTrp: 0.706 ± 0.01	1.361ValTyr: 1.361 ± 0.016	0.0ValXaa: 0.0 ± 0.0
Trp	0.873TrpAla: 0.873 ± 0.013	0.184TrpCys: 0.184 ± 0.005	0.718TrpAsp: 0.718 ± 0.011	0.725TrpGlu: 0.725 ± 0.011	0.43TrpPhe: 0.43 ± 0.008	0.66TrpGly: 0.66 ± 0.01	0.285TrpHis: 0.285 ± 0.006	0.722TrpIle: 0.722 ± 0.012	0.866TrpLys: 0.866 ± 0.014	1.221TrpLeu: 1.221 ± 0.015	0.315TrpMet: 0.315 ± 0.007	0.706TrpAsn: 0.706 ± 0.012	0.521TrpPro: 0.521 ± 0.01	0.475TrpGln: 0.475 ± 0.009	0.716TrpArg: 0.716 ± 0.012	1.013TrpSer: 1.013 ± 0.013	0.762TrpThr: 0.762 ± 0.012	0.625TrpVal: 0.625 ± 0.01	0.183TrpTrp: 0.183 ± 0.005	0.298TrpTyr: 0.298 ± 0.008	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.415TyrAla: 1.415 ± 0.017	0.387TyrCys: 0.387 ± 0.007	1.298TyrAsp: 1.298 ± 0.016	1.232TyrGlu: 1.232 ± 0.014	1.026TyrPhe: 1.026 ± 0.014	1.532TyrGly: 1.532 ± 0.023	0.826TyrHis: 0.826 ± 0.012	1.142TyrIle: 1.142 ± 0.014	1.119TyrLys: 1.119 ± 0.014	2.552TyrLeu: 2.552 ± 0.021	0.47TyrMet: 0.47 ± 0.01	1.104TyrAsn: 1.104 ± 0.015	1.484TyrPro: 1.484 ± 0.018	1.234TyrGln: 1.234 ± 0.014	1.424TyrArg: 1.424 ± 0.015	2.004TyrSer: 2.004 ± 0.017	1.161TyrThr: 1.161 ± 0.014	1.162TyrVal: 1.162 ± 0.013	0.344TyrTrp: 0.344 ± 0.008	0.779TyrTyr: 0.779 ± 0.012	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 15808 proteins (6249486 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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