Leptospira borgpetersenii str. 200701203

Taxonomy: cellular organisms; Bacteria; Spirochaetes; Spirochaetia; Leptospirales; Leptospiraceae; Leptospira; Leptospira borgpetersenii

Average proteome isoelectric point is 7.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4773 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M3GY98|M3GY98_LEPBO Flagellar biosynthetic protein FliQ OS=Leptospira borgpetersenii str. 200701203 OX=1193007 GN=fliQ PE=3 SV=1
MM1 pKa = 7.93DD2 pKa = 5.91ADD4 pKa = 3.76KK5 pKa = 11.2EE6 pKa = 4.36NYY8 pKa = 8.32PNYY11 pKa = 10.41YY12 pKa = 9.82PLILQLLKK20 pKa = 10.87PGGLLIADD28 pKa = 3.61NVLWDD33 pKa = 4.07GSVIAPDD40 pKa = 3.49HH41 pKa = 6.15QEE43 pKa = 3.74PSTIGTPNTFF53 pKa = 3.17

Molecular weight: 5.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M3GKX9|M3GKX9_LEPBO Uncharacterized protein OS=Leptospira borgpetersenii str. 200701203 OX=1193007 GN=LEP1GSC123_2997 PE=4 SV=1
MM1 pKa = 7.02KK2 pKa = 10.22QISFFWNPGLLCVLVFLVTFFFSWIYY28 pKa = 10.17LHH30 pKa = 6.46SKK32 pKa = 9.99RR33 pKa = 11.84FAISDD38 pKa = 3.4ISNEE42 pKa = 3.72RR43 pKa = 11.84SMHH46 pKa = 5.41VGTTKK51 pKa = 10.56KK52 pKa = 10.65SGGIWIFLPVFCLAAIWFGGFEE74 pKa = 3.96IPDD77 pKa = 3.47RR78 pKa = 11.84KK79 pKa = 10.01ILLFLRR85 pKa = 11.84VCCSFSRR92 pKa = 11.84SAFRR96 pKa = 11.84TIYY99 pKa = 9.83FLSVRR104 pKa = 11.84ASVSFWNFFPTSSFPPGTDD123 pKa = 3.08SIFVFGFRR131 pKa = 11.84HH132 pKa = 6.22RR133 pKa = 11.84KK134 pKa = 8.81RR135 pKa = 11.84SGDD138 pKa = 3.3FHH140 pKa = 8.82VDD142 pKa = 3.81FDD144 pKa = 3.78RR145 pKa = 11.84LRR147 pKa = 11.84FVRR150 pKa = 11.84RR151 pKa = 3.34

Molecular weight: 17.78 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4773

0

4773

1094403

33

2305

229.3

25.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.486 ± 0.04

0.863 ± 0.014

4.73 ± 0.024

7.119 ± 0.044

5.795 ± 0.042

6.603 ± 0.041

1.68 ± 0.018

7.753 ± 0.036

7.686 ± 0.038

10.279 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.05 ± 0.016

4.834 ± 0.032

3.933 ± 0.025

3.175 ± 0.023

4.896 ± 0.031

7.756 ± 0.039

4.97 ± 0.032

5.805 ± 0.028

1.081 ± 0.015

3.507 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski