Simian adenovirus 3
Average proteome isoelectric point is 6.59
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 34 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q695Q3|Q695Q3_9ADEN Isoform of Q695Q7 E4 ORF1 OS=Simian adenovirus 3 OX=38420 GN=E4 PE=4 SV=1
MM1 pKa = 7.87 RR2 pKa = 11.84 NFLLSPGLPATVAAEE17 pKa = 3.97 LLEE20 pKa = 5.42 DD21 pKa = 3.79 IVTGALGDD29 pKa = 3.78 EE30 pKa = 4.42 PQVISHH36 pKa = 6.57 FCEE39 pKa = 4.58 DD40 pKa = 4.2 FSLHH44 pKa = 6.83 DD45 pKa = 5.55 LYY47 pKa = 11.28 DD48 pKa = 4.02 IDD50 pKa = 5.51 PGVEE54 pKa = 4.09 GQADD58 pKa = 3.67 EE59 pKa = 4.34 WLEE62 pKa = 4.07 SVDD65 pKa = 5.06 GFFPDD70 pKa = 4.93 AMLLEE75 pKa = 5.06 ADD77 pKa = 5.18 LPPSHH82 pKa = 6.94 NSNTEE87 pKa = 3.9 PEE89 pKa = 4.37 SAAIPQLSSGEE100 pKa = 4.2 LDD102 pKa = 3.56 LACYY106 pKa = 7.85 EE107 pKa = 4.29 TMPPEE112 pKa = 4.3 SDD114 pKa = 4.0 EE115 pKa = 4.2 EE116 pKa = 4.34 DD117 pKa = 3.49 SGISNPTDD125 pKa = 3.38 YY126 pKa = 10.37 MVSKK130 pKa = 10.84 AIAILKK136 pKa = 10.16 EE137 pKa = 4.3 DD138 pKa = 5.31 DD139 pKa = 4.82 DD140 pKa = 6.93 DD141 pKa = 7.13 GDD143 pKa = 4.14 DD144 pKa = 4.83 GFRR147 pKa = 11.84 LDD149 pKa = 4.08 APAVPGRR156 pKa = 11.84 DD157 pKa = 3.41 CKK159 pKa = 10.14 SCEE162 pKa = 3.74 YY163 pKa = 10.35 HH164 pKa = 7.11 RR165 pKa = 11.84 EE166 pKa = 4.08 RR167 pKa = 11.84 TGDD170 pKa = 3.4 PCMLCSLCYY179 pKa = 10.36 LRR181 pKa = 11.84 LNAAFVYY188 pKa = 10.67 SPVSDD193 pKa = 3.8 VEE195 pKa = 4.59 EE196 pKa = 4.72 PDD198 pKa = 3.39 STTGNEE204 pKa = 4.11 EE205 pKa = 4.27 EE206 pKa = 5.01 KK207 pKa = 10.6 PSPPKK212 pKa = 9.52 LTQRR216 pKa = 11.84 CRR218 pKa = 11.84 PNILRR223 pKa = 11.84 PSAQRR228 pKa = 11.84 VSSRR232 pKa = 11.84 KK233 pKa = 8.93 RR234 pKa = 11.84 AAVNCIEE241 pKa = 5.07 DD242 pKa = 4.13 LLEE245 pKa = 4.59 EE246 pKa = 4.21 PTEE249 pKa = 4.11 PLDD252 pKa = 4.98 LSLKK256 pKa = 10.28 RR257 pKa = 11.84 PRR259 pKa = 11.84 PQQ261 pKa = 3.17
Molecular weight: 28.71 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.169
IPC2_protein 4.088
IPC_protein 4.075
Toseland 3.872
ProMoST 4.202
Dawson 4.037
Bjellqvist 4.19
Wikipedia 3.935
Rodwell 3.897
Grimsley 3.783
Solomon 4.037
Lehninger 3.986
Nozaki 4.139
DTASelect 4.342
Thurlkill 3.897
EMBOSS 3.948
Sillero 4.177
Patrickios 3.363
IPC_peptide 4.037
IPC2_peptide 4.164
IPC2.peptide.svr19 4.102
Protein with the highest isoelectric point:
>tr|Q695S7|Q695S7_9ADEN Penton protein OS=Simian adenovirus 3 OX=38420 GN=L2 PE=2 SV=1
MM1 pKa = 7.65 GKK3 pKa = 8.39 LTCRR7 pKa = 11.84 LRR9 pKa = 11.84 MAVPGYY15 pKa = 9.66 RR16 pKa = 11.84 GRR18 pKa = 11.84 SRR20 pKa = 11.84 RR21 pKa = 11.84 RR22 pKa = 11.84 RR23 pKa = 11.84 TLGGSGLRR31 pKa = 11.84 RR32 pKa = 11.84 RR33 pKa = 11.84 RR34 pKa = 11.84 AVHH37 pKa = 6.26 RR38 pKa = 11.84 RR39 pKa = 11.84 LKK41 pKa = 10.98 GGILPALIPIIAAAIGAIPGIASVAVQASQRR72 pKa = 11.84 HH73 pKa = 4.3
Molecular weight: 7.88 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.397
IPC2_protein 10.994
IPC_protein 12.442
Toseland 12.603
ProMoST 13.1
Dawson 12.603
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.164
Grimsley 12.647
Solomon 13.1
Lehninger 12.998
Nozaki 12.603
DTASelect 12.603
Thurlkill 12.603
EMBOSS 13.1
Sillero 12.603
Patrickios 11.901
IPC_peptide 13.1
IPC2_peptide 12.091
IPC2.peptide.svr19 9.128
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
18
16
34
11135
73
1169
327.5
36.78
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.921 ± 0.423
1.94 ± 0.282
4.733 ± 0.277
6.098 ± 0.536
4.041 ± 0.349
6.107 ± 0.373
2.371 ± 0.243
3.96 ± 0.268
3.404 ± 0.329
9.897 ± 0.534
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.209 ± 0.241
4.652 ± 0.513
6.87 ± 0.346
4.41 ± 0.232
7.319 ± 0.608
6.87 ± 0.337
5.855 ± 0.404
6.412 ± 0.38
1.383 ± 0.085
3.547 ± 0.308
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here