Nesiotobacter exalbescens

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Stappiaceae; Nesiotobacter

Average proteome isoelectric point is 6.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3634 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1U7JF22|A0A1U7JF22_9RHOB ABC transporter domain-containing protein OS=Nesiotobacter exalbescens OX=197461 GN=A3843_14080 PE=4 SV=1
MM1 pKa = 7.52KK2 pKa = 10.58LKK4 pKa = 10.83ALLLATAGAAVATSVSAADD23 pKa = 3.89LPVVAEE29 pKa = 4.07PVDD32 pKa = 3.84YY33 pKa = 11.35VQVCDD38 pKa = 4.02AYY40 pKa = 10.52GAGFFKK46 pKa = 10.7IPGKK50 pKa = 8.57DD51 pKa = 2.93TCLKK55 pKa = 9.04IGGRR59 pKa = 11.84IRR61 pKa = 11.84TQIVSDD67 pKa = 4.29NLDD70 pKa = 3.06SDD72 pKa = 4.88ADD74 pKa = 3.87YY75 pKa = 11.59DD76 pKa = 4.69DD77 pKa = 4.26YY78 pKa = 11.8SAYY81 pKa = 10.4ARR83 pKa = 11.84GYY85 pKa = 10.86LYY87 pKa = 9.46LTSMTDD93 pKa = 3.3TEE95 pKa = 4.12IGLIKK100 pKa = 9.96TYY102 pKa = 10.27TEE104 pKa = 5.68FNSEE108 pKa = 3.86WNQDD112 pKa = 3.62GGDD115 pKa = 3.7GSVDD119 pKa = 3.19VGDD122 pKa = 5.12AYY124 pKa = 11.33LQIGTNFADD133 pKa = 4.14VIIGRR138 pKa = 11.84TTSLFDD144 pKa = 3.65GFTGAVDD151 pKa = 3.23VGVIGRR157 pKa = 11.84NWSDD161 pKa = 2.95QGTLQFALTKK171 pKa = 10.58SLGNGVTAAVSAEE184 pKa = 3.82DD185 pKa = 3.13SSYY188 pKa = 11.53RR189 pKa = 11.84DD190 pKa = 3.17GDD192 pKa = 4.11TNSADD197 pKa = 3.76YY198 pKa = 10.89VGKK201 pKa = 10.64LGLAQGWGSVEE212 pKa = 3.9VAAALHH218 pKa = 6.04DD219 pKa = 4.32TVAGAVDD226 pKa = 3.4NYY228 pKa = 10.43GYY230 pKa = 11.01AVRR233 pKa = 11.84GTATVNLDD241 pKa = 3.14MLAAGDD247 pKa = 3.89AFVFQAAYY255 pKa = 10.51ADD257 pKa = 3.79AASAYY262 pKa = 9.74LGKK265 pKa = 8.88STAAWDD271 pKa = 3.9LAGDD275 pKa = 3.99EE276 pKa = 4.4AWALGAGFTHH286 pKa = 6.73YY287 pKa = 7.53WTSEE291 pKa = 3.41IATSIDD297 pKa = 3.05GSYY300 pKa = 10.79LSNDD304 pKa = 3.37DD305 pKa = 3.91ADD307 pKa = 3.95YY308 pKa = 11.42DD309 pKa = 3.38RR310 pKa = 11.84WAIDD314 pKa = 4.23GSVGWYY320 pKa = 8.06PVSGMVVALDD330 pKa = 3.52GGFASEE336 pKa = 4.42DD337 pKa = 3.59AAGVEE342 pKa = 4.02ADD344 pKa = 3.71EE345 pKa = 4.68AKK347 pKa = 10.4IGARR351 pKa = 11.84VQYY354 pKa = 9.48TFF356 pKa = 4.12

Molecular weight: 37.22 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1U7JK17|A0A1U7JK17_9RHOB UPF0229 protein A3843_04610 OS=Nesiotobacter exalbescens OX=197461 GN=A3843_04610 PE=3 SV=1
MM1 pKa = 7.13QAPKK5 pKa = 10.1FFPGSGFPTSSVIRR19 pKa = 11.84LAARR23 pKa = 11.84YY24 pKa = 9.15RR25 pKa = 11.84EE26 pKa = 4.19AGVFPTYY33 pKa = 10.92DD34 pKa = 3.46PAVLMMALRR43 pKa = 11.84MPEE46 pKa = 4.06RR47 pKa = 11.84APEE50 pKa = 4.13ALLAEE55 pKa = 4.35ARR57 pKa = 11.84HH58 pKa = 5.69INRR61 pKa = 11.84TGRR64 pKa = 11.84WQNEE68 pKa = 3.41EE69 pKa = 3.29EE70 pKa = 4.13ARR72 pKa = 11.84RR73 pKa = 11.84KK74 pKa = 9.18RR75 pKa = 11.84RR76 pKa = 11.84KK77 pKa = 9.45ASPRR81 pKa = 11.84KK82 pKa = 8.16PSVKK86 pKa = 10.24ARR88 pKa = 11.84VEE90 pKa = 4.19KK91 pKa = 10.52ILQQEE96 pKa = 3.94AAEE99 pKa = 4.38AGISVEE105 pKa = 4.12RR106 pKa = 11.84LRR108 pKa = 11.84GRR110 pKa = 11.84CRR112 pKa = 11.84KK113 pKa = 9.82RR114 pKa = 11.84PSLQARR120 pKa = 11.84RR121 pKa = 11.84EE122 pKa = 4.14AVARR126 pKa = 11.84CHH128 pKa = 7.25DD129 pKa = 4.2EE130 pKa = 5.26LGLTCKK136 pKa = 10.57QIGEE140 pKa = 4.34YY141 pKa = 10.51FSGRR145 pKa = 11.84GAEE148 pKa = 4.09AVRR151 pKa = 11.84TLLHH155 pKa = 6.56RR156 pKa = 11.84AQAARR161 pKa = 11.84QQQAAEE167 pKa = 4.33DD168 pKa = 3.95DD169 pKa = 3.96VHH171 pKa = 8.76RR172 pKa = 11.84LMEE175 pKa = 4.32MLTEE179 pKa = 4.52AEE181 pKa = 4.21RR182 pKa = 11.84KK183 pKa = 9.42SLKK186 pKa = 9.86QRR188 pKa = 11.84ALALTIAEE196 pKa = 4.62EE197 pKa = 4.31IACKK201 pKa = 10.25HH202 pKa = 5.46EE203 pKa = 4.1RR204 pKa = 11.84TLAEE208 pKa = 3.17IRR210 pKa = 11.84ARR212 pKa = 11.84RR213 pKa = 11.84RR214 pKa = 11.84APALHH219 pKa = 5.79GVRR222 pKa = 11.84RR223 pKa = 11.84EE224 pKa = 3.83ALYY227 pKa = 9.44WAWEE231 pKa = 4.08RR232 pKa = 11.84TNLGWSEE239 pKa = 3.89LGQLFGRR246 pKa = 11.84GAGHH250 pKa = 5.8VAGLVRR256 pKa = 11.84EE257 pKa = 4.13YY258 pKa = 11.56AEE260 pKa = 4.12LHH262 pKa = 6.09GLRR265 pKa = 11.84APEE268 pKa = 3.99GMGRR272 pKa = 11.84VAAA275 pKa = 4.82

Molecular weight: 31.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3634

0

3634

1186005

38

3381

326.4

35.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.905 ± 0.048

0.917 ± 0.012

5.7 ± 0.043

6.355 ± 0.039

3.914 ± 0.03

8.07 ± 0.042

2.079 ± 0.019

5.401 ± 0.028

3.856 ± 0.034

10.195 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.642 ± 0.023

3.062 ± 0.024

4.717 ± 0.027

3.621 ± 0.025

6.045 ± 0.038

5.847 ± 0.032

5.552 ± 0.029

7.463 ± 0.036

1.217 ± 0.017

2.442 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski