Desulfacinum hydrothermale DSM 13146
Average proteome isoelectric point is 6.94
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3262 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1W1X0J0|A0A1W1X0J0_9DELT Uncharacterized protein OS=Desulfacinum hydrothermale DSM 13146 OX=1121390 GN=SAMN02746041_00270 PE=4 SV=1
MM1 pKa = 7.81 RR2 pKa = 11.84 KK3 pKa = 9.32 NLVVLLAVLMAVAIALPAFAVDD25 pKa = 4.18 FKK27 pKa = 10.63 YY28 pKa = 11.02 GGMMRR33 pKa = 11.84 VRR35 pKa = 11.84 WYY37 pKa = 10.73 SYY39 pKa = 11.51 NNFDD43 pKa = 4.46 GTDD46 pKa = 3.54 DD47 pKa = 4.83 ADD49 pKa = 3.54 DD50 pKa = 3.88 HH51 pKa = 7.25 ANYY54 pKa = 10.02 FDD56 pKa = 3.33 QRR58 pKa = 11.84 VRR60 pKa = 11.84 MYY62 pKa = 8.27 FTFVASEE69 pKa = 3.81 NLQLVTKK76 pKa = 10.11 WEE78 pKa = 4.03 EE79 pKa = 4.08 DD80 pKa = 3.81 NVWGAKK86 pKa = 10.26 GGDD89 pKa = 3.39 RR90 pKa = 11.84 DD91 pKa = 3.98 LTHH94 pKa = 6.61 ATSDD98 pKa = 3.41 FRR100 pKa = 11.84 DD101 pKa = 4.25 PYY103 pKa = 11.82 DD104 pKa = 3.48 MEE106 pKa = 4.54 TKK108 pKa = 10.38 NVYY111 pKa = 10.74 LDD113 pKa = 3.9 FNIPNTPIRR122 pKa = 11.84 AKK124 pKa = 10.51 LGQQGFGLFGGRR136 pKa = 11.84 IIAEE140 pKa = 4.7 DD141 pKa = 3.41 YY142 pKa = 10.32 TGAKK146 pKa = 10.06 FSMPFDD152 pKa = 3.84 PVKK155 pKa = 10.45 VTLGYY160 pKa = 9.99 IAEE163 pKa = 4.22 QNVDD167 pKa = 3.46 YY168 pKa = 11.09 SSEE171 pKa = 3.84 KK172 pKa = 11.05 DD173 pKa = 3.57 NVDD176 pKa = 2.52 IYY178 pKa = 11.43 YY179 pKa = 10.48 LQLQYY184 pKa = 11.85 ANGPFSGEE192 pKa = 4.39 LIGLYY197 pKa = 9.94 QDD199 pKa = 4.34 GSDD202 pKa = 3.38 TAVSLKK208 pKa = 9.91 TEE210 pKa = 4.12 YY211 pKa = 11.06 ADD213 pKa = 4.5 PGVDD217 pKa = 3.07 AQGRR221 pKa = 11.84 EE222 pKa = 4.22 TYY224 pKa = 10.42 DD225 pKa = 3.08 GYY227 pKa = 9.68 TFEE230 pKa = 5.94 DD231 pKa = 3.05 NHH233 pKa = 7.91 LFDD236 pKa = 5.97 LGVKK240 pKa = 9.85 LAYY243 pKa = 10.34 SSDD246 pKa = 3.35 MFKK249 pKa = 10.48 ATVHH253 pKa = 6.49 FIKK256 pKa = 10.83 NFGSVDD262 pKa = 4.05 LNGEE266 pKa = 3.99 SADD269 pKa = 3.69 YY270 pKa = 10.82 KK271 pKa = 11.08 GWFLGADD278 pKa = 3.26 VAIPVNDD285 pKa = 3.61 FTFGLGGFYY294 pKa = 7.96 TTGQDD299 pKa = 3.34 EE300 pKa = 4.6 GDD302 pKa = 4.33 DD303 pKa = 3.83 DD304 pKa = 4.8 VEE306 pKa = 5.03 GYY308 pKa = 10.29 VYY310 pKa = 10.43 PGAVGDD316 pKa = 3.85 SFYY319 pKa = 10.25 WAEE322 pKa = 3.68 IMGFGVLDD330 pKa = 4.13 DD331 pKa = 4.04 MTPRR335 pKa = 11.84 AYY337 pKa = 9.29 TGYY340 pKa = 9.22 PGAGEE345 pKa = 4.0 YY346 pKa = 8.71 TAGGNPSNLYY356 pKa = 8.99 TLYY359 pKa = 11.2 ASVDD363 pKa = 3.42 WQALEE368 pKa = 3.79 ATKK371 pKa = 10.78 LHH373 pKa = 6.11 LAYY376 pKa = 10.3 YY377 pKa = 9.72 YY378 pKa = 10.69 LGTAEE383 pKa = 5.03 DD384 pKa = 4.19 VVANAATGEE393 pKa = 4.16 KK394 pKa = 9.69 DD395 pKa = 3.38 DD396 pKa = 4.38 SVGSEE401 pKa = 3.49 VDD403 pKa = 3.96 FRR405 pKa = 11.84 LTQKK409 pKa = 10.38 VVDD412 pKa = 4.32 GLNLDD417 pKa = 3.83 VIGAYY422 pKa = 10.49 LFADD426 pKa = 4.04 DD427 pKa = 5.34 AFSGTKK433 pKa = 10.29 DD434 pKa = 3.71 DD435 pKa = 4.34 DD436 pKa = 3.85 DD437 pKa = 6.18 AYY439 pKa = 10.59 EE440 pKa = 4.17 VGARR444 pKa = 11.84 LQWAFF449 pKa = 3.54
Molecular weight: 49.76 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.764
IPC2_protein 3.986
IPC_protein 4.024
Toseland 3.795
ProMoST 4.177
Dawson 4.012
Bjellqvist 4.164
Wikipedia 3.961
Rodwell 3.846
Grimsley 3.694
Solomon 4.012
Lehninger 3.973
Nozaki 4.126
DTASelect 4.406
Thurlkill 3.846
EMBOSS 3.961
Sillero 4.139
Patrickios 1.507
IPC_peptide 4.012
IPC2_peptide 4.126
IPC2.peptide.svr19 4.021
Protein with the highest isoelectric point:
>tr|A0A1W1XIP4|A0A1W1XIP4_9DELT Magnesium chelatase family protein OS=Desulfacinum hydrothermale DSM 13146 OX=1121390 GN=SAMN02746041_01807 PE=3 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.44 RR3 pKa = 11.84 TYY5 pKa = 10.19 QPSNIKK11 pKa = 10.14 RR12 pKa = 11.84 KK13 pKa = 7.89 RR14 pKa = 11.84 THH16 pKa = 5.96 GFLVRR21 pKa = 11.84 MSTKK25 pKa = 10.41 AGRR28 pKa = 11.84 QVIKK32 pKa = 10.42 RR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.74 GRR39 pKa = 11.84 KK40 pKa = 8.66 RR41 pKa = 11.84 LAVV44 pKa = 3.41
Molecular weight: 5.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.414
IPC2_protein 11.096
IPC_protein 12.457
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.384
Grimsley 12.661
Solomon 13.1
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.106
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.021
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3262
0
3262
1058542
39
2151
324.5
36.04
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.607 ± 0.038
1.347 ± 0.018
5.239 ± 0.028
6.699 ± 0.041
3.805 ± 0.028
7.878 ± 0.036
2.424 ± 0.019
4.839 ± 0.032
4.101 ± 0.035
10.862 ± 0.051
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.395 ± 0.02
2.387 ± 0.021
5.295 ± 0.025
3.628 ± 0.025
7.81 ± 0.047
5.038 ± 0.028
4.67 ± 0.024
8.027 ± 0.037
1.369 ± 0.019
2.579 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here