Gemmiger sp. An120

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Eubacteriales incertae sedis; Gemmiger; unclassified Gemmiger

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3048 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Y4TL65|A0A1Y4TL65_9FIRM Peptide ABC transporter permease OS=Gemmiger sp. An120 OX=1965549 GN=B5E65_09290 PE=3 SV=1
MM1 pKa = 7.3NRR3 pKa = 11.84TDD5 pKa = 3.31IIKK8 pKa = 10.53RR9 pKa = 11.84IAAGVLAAVLTAALAGCGKK28 pKa = 8.44TPASSVGGQDD38 pKa = 2.83TFEE41 pKa = 5.14PDD43 pKa = 3.27SAVSTTGAWRR53 pKa = 11.84EE54 pKa = 4.03EE55 pKa = 4.24EE56 pKa = 4.23VTPDD60 pKa = 3.03EE61 pKa = 4.29NARR64 pKa = 11.84SYY66 pKa = 8.17KK67 pKa = 10.29TPVTLADD74 pKa = 3.53GSVRR78 pKa = 11.84MLEE81 pKa = 4.06AGADD85 pKa = 3.77NTLHH89 pKa = 7.12LLTSADD95 pKa = 5.03NGATWQEE102 pKa = 4.11ITVDD106 pKa = 3.55WQPAGAEE113 pKa = 4.23SFGVCRR119 pKa = 11.84MLPDD123 pKa = 4.17GGCFVTAVKK132 pKa = 10.3EE133 pKa = 4.21DD134 pKa = 3.02NGQRR138 pKa = 11.84KK139 pKa = 8.19EE140 pKa = 4.37SYY142 pKa = 7.35WTLDD146 pKa = 3.25PEE148 pKa = 4.66TGVLDD153 pKa = 4.13PVTLPEE159 pKa = 5.08NIVQLSDD166 pKa = 3.62AYY168 pKa = 10.07PLGGDD173 pKa = 3.66SLLLLGMSLVPASADD188 pKa = 3.3APAVLVDD195 pKa = 3.98EE196 pKa = 4.74NGMMKK201 pKa = 8.06TTSPWKK207 pKa = 10.44LDD209 pKa = 3.76LATGACEE216 pKa = 4.4GLTALTNTLQGSIISGMAADD236 pKa = 3.96PTEE239 pKa = 4.08LGAYY243 pKa = 9.78YY244 pKa = 10.61SLAYY248 pKa = 9.5MDD250 pKa = 4.26TGVQLLRR257 pKa = 11.84TDD259 pKa = 3.62ADD261 pKa = 3.6EE262 pKa = 4.41ATACVFDD269 pKa = 4.31SLPNASAAAFAACADD284 pKa = 3.7AEE286 pKa = 4.42GNYY289 pKa = 10.38YY290 pKa = 9.83YY291 pKa = 10.98ASSKK295 pKa = 10.55GIYY298 pKa = 9.26RR299 pKa = 11.84VAKK302 pKa = 10.47GGDD305 pKa = 3.36LAEE308 pKa = 4.43LVVDD312 pKa = 4.92GASTALSRR320 pKa = 11.84SEE322 pKa = 3.95AVVMGLTCCADD333 pKa = 3.31GSFLTVSLEE342 pKa = 4.06GGQDD346 pKa = 2.86AMTAHH351 pKa = 8.4LYY353 pKa = 9.78RR354 pKa = 11.84YY355 pKa = 9.24YY356 pKa = 10.39WDD358 pKa = 3.97ADD360 pKa = 3.5APAEE364 pKa = 4.08EE365 pKa = 4.51STAALTVWSLEE376 pKa = 4.06NNDD379 pKa = 3.46TVRR382 pKa = 11.84AAIEE386 pKa = 4.23VYY388 pKa = 9.51EE389 pKa = 4.01QQNPGLTVTYY399 pKa = 9.94QVAVEE404 pKa = 4.71DD405 pKa = 4.37GATTRR410 pKa = 11.84EE411 pKa = 4.17DD412 pKa = 3.58ALSALNTALLAGSGPDD428 pKa = 3.61VLILDD433 pKa = 4.11GLDD436 pKa = 2.76WQAYY440 pKa = 7.83RR441 pKa = 11.84DD442 pKa = 3.85KK443 pKa = 11.51GLLADD448 pKa = 4.2LSEE451 pKa = 4.23WVDD454 pKa = 4.38LSALQSNLVDD464 pKa = 3.59PFRR467 pKa = 11.84EE468 pKa = 4.0QEE470 pKa = 4.34GVFVLPARR478 pKa = 11.84FSVPVLCGAPEE489 pKa = 4.22DD490 pKa = 4.18LAGLDD495 pKa = 3.91SLDD498 pKa = 3.89ALAEE502 pKa = 4.06YY503 pKa = 10.72LLQLPARR510 pKa = 11.84PAWDD514 pKa = 3.62YY515 pKa = 11.31TSAGYY520 pKa = 10.1YY521 pKa = 8.88EE522 pKa = 4.53QLEE525 pKa = 4.32PPYY528 pKa = 10.68GLGFVSVEE536 pKa = 3.77QLLDD540 pKa = 3.79FVLGSSAAALTEE552 pKa = 4.6DD553 pKa = 4.41GANGDD558 pKa = 3.5AVEE561 pKa = 4.37AVLKK565 pKa = 9.76FVQQVGRR572 pKa = 11.84YY573 pKa = 9.67YY574 pKa = 11.44GMEE577 pKa = 4.27DD578 pKa = 3.5YY579 pKa = 10.88KK580 pKa = 11.49GLLSNGVISSGSEE593 pKa = 4.05GQSVSYY599 pKa = 11.36GDD601 pKa = 3.76GSYY604 pKa = 11.16EE605 pKa = 4.37CFSTGNALLAWEE617 pKa = 4.41EE618 pKa = 4.17MLTPSYY624 pKa = 9.61LTAVRR629 pKa = 11.84KK630 pKa = 9.98DD631 pKa = 3.31RR632 pKa = 11.84AGVSLIQRR640 pKa = 11.84PGLVQGAYY648 pKa = 8.55TPRR651 pKa = 11.84TLAAVCADD659 pKa = 3.18ASEE662 pKa = 4.12EE663 pKa = 3.92AGAFVAILFGSEE675 pKa = 4.28VQASWQQDD683 pKa = 3.63GMPVRR688 pKa = 11.84ADD690 pKa = 3.37ALQSSIEE697 pKa = 4.18ANCPDD702 pKa = 3.67PEE704 pKa = 4.44ARR706 pKa = 11.84QNVQALIDD714 pKa = 3.99ALQTPVTQPDD724 pKa = 3.57DD725 pKa = 3.8TVRR728 pKa = 11.84DD729 pKa = 3.71ALLQNANALIAGDD742 pKa = 3.67VTLEE746 pKa = 3.81QARR749 pKa = 11.84TGVEE753 pKa = 3.96DD754 pKa = 4.41ALNLYY759 pKa = 9.86LAEE762 pKa = 4.19QEE764 pKa = 4.22

Molecular weight: 80.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Y4TER5|A0A1Y4TER5_9FIRM Ribonuclease P protein component OS=Gemmiger sp. An120 OX=1965549 GN=rnpA PE=3 SV=1
MM1 pKa = 7.36KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 9.67QPKK8 pKa = 9.27KK9 pKa = 7.52RR10 pKa = 11.84QRR12 pKa = 11.84SAVHH16 pKa = 6.23GFLNRR21 pKa = 11.84MSSRR25 pKa = 11.84SGRR28 pKa = 11.84KK29 pKa = 8.66VIKK32 pKa = 10.16ARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.35GRR39 pKa = 11.84AKK41 pKa = 10.44LAAA44 pKa = 4.52

Molecular weight: 5.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3048

0

3048

991804

25

2252

325.4

35.84

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.614 ± 0.072

1.746 ± 0.018

5.403 ± 0.032

6.426 ± 0.038

3.806 ± 0.029

7.962 ± 0.042

1.719 ± 0.019

5.008 ± 0.041

4.034 ± 0.042

10.607 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.67 ± 0.022

3.092 ± 0.027

4.547 ± 0.03

3.827 ± 0.03

6.01 ± 0.045

5.137 ± 0.033

5.469 ± 0.031

7.191 ± 0.035

1.272 ± 0.019

3.459 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski