Amino acid dipepetide frequency for Gemmiger sp. An120

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
15.337AlaAla: 15.337 ± 0.227
1.834AlaCys: 1.834 ± 0.047
6.026AlaAsp: 6.026 ± 0.081
6.591AlaGlu: 6.591 ± 0.091
3.692AlaPhe: 3.692 ± 0.067
9.618AlaGly: 9.618 ± 0.128
1.583AlaHis: 1.583 ± 0.042
4.717AlaIle: 4.717 ± 0.077
3.536AlaLys: 3.536 ± 0.069
11.644AlaLeu: 11.644 ± 0.128
2.817AlaMet: 2.817 ± 0.056
2.482AlaAsn: 2.482 ± 0.053
4.191AlaPro: 4.191 ± 0.084
4.155AlaGln: 4.155 ± 0.069
6.728AlaArg: 6.728 ± 0.095
4.494AlaSer: 4.494 ± 0.064
3.952AlaThr: 3.952 ± 0.067
8.651AlaVal: 8.651 ± 0.105
1.318AlaTrp: 1.318 ± 0.044
2.771AlaTyr: 2.771 ± 0.056
0.0AlaXaa: 0.0 ± 0.0
Cys
1.797CysAla: 1.797 ± 0.042
0.35CysCys: 0.35 ± 0.02
0.851CysAsp: 0.851 ± 0.032
0.923CysGlu: 0.923 ± 0.028
0.656CysPhe: 0.656 ± 0.031
1.93CysGly: 1.93 ± 0.054
0.356CysHis: 0.356 ± 0.018
0.86CysIle: 0.86 ± 0.035
0.564CysLys: 0.564 ± 0.029
1.722CysLeu: 1.722 ± 0.041
0.374CysMet: 0.374 ± 0.021
0.5CysAsn: 0.5 ± 0.022
0.935CysPro: 0.935 ± 0.037
0.539CysGln: 0.539 ± 0.023
1.34CysArg: 1.34 ± 0.041
0.906CysSer: 0.906 ± 0.03
0.906CysThr: 0.906 ± 0.032
1.184CysVal: 1.184 ± 0.037
0.211CysTrp: 0.211 ± 0.013
0.56CysTyr: 0.56 ± 0.026
0.0CysXaa: 0.0 ± 0.0
Asp
5.299AspAla: 5.299 ± 0.086
0.858AspCys: 0.858 ± 0.03
2.373AspAsp: 2.373 ± 0.064
4.243AspGlu: 4.243 ± 0.069
2.192AspPhe: 2.192 ± 0.049
4.847AspGly: 4.847 ± 0.082
0.891AspHis: 0.891 ± 0.031
2.806AspIle: 2.806 ± 0.057
2.469AspLys: 2.469 ± 0.061
5.014AspLeu: 5.014 ± 0.083
1.483AspMet: 1.483 ± 0.036
1.47AspAsn: 1.47 ± 0.038
2.948AspPro: 2.948 ± 0.056
1.551AspGln: 1.551 ± 0.038
3.093AspArg: 3.093 ± 0.063
2.682AspSer: 2.682 ± 0.056
3.247AspThr: 3.247 ± 0.059
3.499AspVal: 3.499 ± 0.065
0.867AspTrp: 0.867 ± 0.028
2.193AspTyr: 2.193 ± 0.051
0.0AspXaa: 0.0 ± 0.0
Glu
5.958GluAla: 5.958 ± 0.087
0.749GluCys: 0.749 ± 0.028
3.6GluAsp: 3.6 ± 0.071
5.028GluGlu: 5.028 ± 0.096
2.202GluPhe: 2.202 ± 0.051
4.486GluGly: 4.486 ± 0.081
1.178GluHis: 1.178 ± 0.035
3.367GluIle: 3.367 ± 0.067
3.799GluLys: 3.799 ± 0.076
6.458GluLeu: 6.458 ± 0.083
2.104GluMet: 2.104 ± 0.05
2.77GluAsn: 2.77 ± 0.058
2.677GluPro: 2.677 ± 0.069
3.446GluGln: 3.446 ± 0.068
3.368GluArg: 3.368 ± 0.063
2.942GluSer: 2.942 ± 0.054
3.442GluThr: 3.442 ± 0.065
3.554GluVal: 3.554 ± 0.059
0.64GluTrp: 0.64 ± 0.026
2.489GluTyr: 2.489 ± 0.05
0.0GluXaa: 0.0 ± 0.0
Phe
3.941PheAla: 3.941 ± 0.067
0.957PheCys: 0.957 ± 0.03
2.386PheAsp: 2.386 ± 0.05
2.12PheGlu: 2.12 ± 0.046
1.711PhePhe: 1.711 ± 0.052
3.215PheGly: 3.215 ± 0.068
0.677PheHis: 0.677 ± 0.029
1.828PheIle: 1.828 ± 0.046
1.187PheLys: 1.187 ± 0.035
3.74PheLeu: 3.74 ± 0.065
0.889PheMet: 0.889 ± 0.03
1.095PheAsn: 1.095 ± 0.036
1.405PhePro: 1.405 ± 0.037
1.057PheGln: 1.057 ± 0.034
1.914PheArg: 1.914 ± 0.044
2.634PheSer: 2.634 ± 0.052
2.535PheThr: 2.535 ± 0.05
2.811PheVal: 2.811 ± 0.056
0.54PheTrp: 0.54 ± 0.025
1.422PheTyr: 1.422 ± 0.038
0.0PheXaa: 0.0 ± 0.0
Gly
7.391GlyAla: 7.391 ± 0.105
1.69GlyCys: 1.69 ± 0.043
3.763GlyAsp: 3.763 ± 0.066
4.988GlyGlu: 4.988 ± 0.079
3.223GlyPhe: 3.223 ± 0.059
6.353GlyGly: 6.353 ± 0.099
1.388GlyHis: 1.388 ± 0.037
4.363GlyIle: 4.363 ± 0.069
3.798GlyLys: 3.798 ± 0.064
8.126GlyLeu: 8.126 ± 0.097
2.517GlyMet: 2.517 ± 0.047
2.359GlyAsn: 2.359 ± 0.05
2.476GlyPro: 2.476 ± 0.06
3.092GlyGln: 3.092 ± 0.054
4.841GlyArg: 4.841 ± 0.081
4.364GlySer: 4.364 ± 0.081
4.328GlyThr: 4.328 ± 0.08
6.314GlyVal: 6.314 ± 0.092
1.215GlyTrp: 1.215 ± 0.035
3.035GlyTyr: 3.035 ± 0.063
0.0GlyXaa: 0.0 ± 0.0
His
1.37HisAla: 1.37 ± 0.037
0.352HisCys: 0.352 ± 0.021
0.841HisAsp: 0.841 ± 0.036
0.856HisGlu: 0.856 ± 0.03
0.754HisPhe: 0.754 ± 0.028
1.335HisGly: 1.335 ± 0.04
0.383HisHis: 0.383 ± 0.027
1.07HisIle: 1.07 ± 0.036
0.695HisLys: 0.695 ± 0.024
1.713HisLeu: 1.713 ± 0.05
0.432HisMet: 0.432 ± 0.021
0.581HisAsn: 0.581 ± 0.024
1.104HisPro: 1.104 ± 0.033
0.567HisGln: 0.567 ± 0.024
1.058HisArg: 1.058 ± 0.035
0.973HisSer: 0.973 ± 0.032
1.166HisThr: 1.166 ± 0.032
0.987HisVal: 0.987 ± 0.033
0.239HisTrp: 0.239 ± 0.015
0.715HisTyr: 0.715 ± 0.027
0.0HisXaa: 0.0 ± 0.0
Ile
4.933IleAla: 4.933 ± 0.076
1.001IleCys: 1.001 ± 0.031
2.758IleAsp: 2.758 ± 0.059
2.802IleGlu: 2.802 ± 0.046
1.834IlePhe: 1.834 ± 0.04
3.807IleGly: 3.807 ± 0.071
0.908IleHis: 0.908 ± 0.03
2.267IleIle: 2.267 ± 0.055
1.744IleLys: 1.744 ± 0.051
5.726IleLeu: 5.726 ± 0.083
1.039IleMet: 1.039 ± 0.032
1.572IleAsn: 1.572 ± 0.042
2.739IlePro: 2.739 ± 0.054
1.873IleGln: 1.873 ± 0.047
3.405IleArg: 3.405 ± 0.065
3.0IleSer: 3.0 ± 0.067
3.184IleThr: 3.184 ± 0.061
3.34IleVal: 3.34 ± 0.059
0.575IleTrp: 0.575 ± 0.027
1.573IleTyr: 1.573 ± 0.043
0.0IleXaa: 0.0 ± 0.0
Lys
4.122LysAla: 4.122 ± 0.076
0.439LysCys: 0.439 ± 0.022
2.021LysAsp: 2.021 ± 0.046
3.041LysGlu: 3.041 ± 0.071
1.071LysPhe: 1.071 ± 0.033
2.893LysGly: 2.893 ± 0.057
0.613LysHis: 0.613 ± 0.024
2.157LysIle: 2.157 ± 0.05
2.657LysLys: 2.657 ± 0.067
3.866LysLeu: 3.866 ± 0.078
1.174LysMet: 1.174 ± 0.037
1.707LysAsn: 1.707 ± 0.041
1.846LysPro: 1.846 ± 0.049
1.683LysGln: 1.683 ± 0.047
2.306LysArg: 2.306 ± 0.056
1.92LysSer: 1.92 ± 0.043
2.398LysThr: 2.398 ± 0.053
2.604LysVal: 2.604 ± 0.06
0.422LysTrp: 0.422 ± 0.023
1.402LysTyr: 1.402 ± 0.038
0.0LysXaa: 0.0 ± 0.0
Leu
12.78LeuAla: 12.78 ± 0.174
2.36LeuCys: 2.36 ± 0.049
6.066LeuAsp: 6.066 ± 0.08
6.369LeuGlu: 6.369 ± 0.083
4.152LeuPhe: 4.152 ± 0.075
7.466LeuGly: 7.466 ± 0.103
1.859LeuHis: 1.859 ± 0.046
4.758LeuIle: 4.758 ± 0.075
3.521LeuLys: 3.521 ± 0.074
11.638LeuLeu: 11.638 ± 0.156
2.469LeuMet: 2.469 ± 0.05
3.09LeuAsn: 3.09 ± 0.053
5.543LeuPro: 5.543 ± 0.084
3.428LeuGln: 3.428 ± 0.064
6.477LeuArg: 6.477 ± 0.092
5.834LeuSer: 5.834 ± 0.084
6.294LeuThr: 6.294 ± 0.106
7.084LeuVal: 7.084 ± 0.099
1.311LeuTrp: 1.311 ± 0.044
3.567LeuTyr: 3.567 ± 0.065
0.0LeuXaa: 0.0 ± 0.0
Met
2.855MetAla: 2.855 ± 0.056
0.264MetCys: 0.264 ± 0.018
1.545MetAsp: 1.545 ± 0.04
1.985MetGlu: 1.985 ± 0.045
0.821MetPhe: 0.821 ± 0.029
2.091MetGly: 2.091 ± 0.054
0.379MetHis: 0.379 ± 0.018
1.28MetIle: 1.28 ± 0.033
1.462MetLys: 1.462 ± 0.041
2.79MetLeu: 2.79 ± 0.055
0.686MetMet: 0.686 ± 0.03
1.06MetAsn: 1.06 ± 0.032
1.331MetPro: 1.331 ± 0.034
1.125MetGln: 1.125 ± 0.035
1.478MetArg: 1.478 ± 0.041
1.37MetSer: 1.37 ± 0.035
1.484MetThr: 1.484 ± 0.035
1.778MetVal: 1.778 ± 0.049
0.215MetTrp: 0.215 ± 0.016
0.705MetTyr: 0.705 ± 0.026
0.0MetXaa: 0.0 ± 0.0
Asn
2.899AsnAla: 2.899 ± 0.056
0.523AsnCys: 0.523 ± 0.023
1.46AsnAsp: 1.46 ± 0.044
1.776AsnGlu: 1.776 ± 0.042
1.18AsnPhe: 1.18 ± 0.033
2.87AsnGly: 2.87 ± 0.052
0.541AsnHis: 0.541 ± 0.024
1.785AsnIle: 1.785 ± 0.045
1.274AsnLys: 1.274 ± 0.036
3.144AsnLeu: 3.144 ± 0.064
0.819AsnMet: 0.819 ± 0.028
1.051AsnAsn: 1.051 ± 0.038
1.776AsnPro: 1.776 ± 0.038
1.069AsnGln: 1.069 ± 0.035
1.85AsnArg: 1.85 ± 0.043
1.545AsnSer: 1.545 ± 0.046
1.888AsnThr: 1.888 ± 0.048
1.928AsnVal: 1.928 ± 0.045
0.395AsnTrp: 0.395 ± 0.022
1.145AsnTyr: 1.145 ± 0.037
0.0AsnXaa: 0.0 ± 0.0
Pro
5.848ProAla: 5.848 ± 0.109
0.696ProCys: 0.696 ± 0.03
3.325ProAsp: 3.325 ± 0.057
3.936ProGlu: 3.936 ± 0.064
1.742ProPhe: 1.742 ± 0.044
3.831ProGly: 3.831 ± 0.067
0.768ProHis: 0.768 ± 0.026
1.799ProIle: 1.799 ± 0.043
1.337ProLys: 1.337 ± 0.041
4.34ProLeu: 4.34 ± 0.071
1.05ProMet: 1.05 ± 0.032
1.187ProAsn: 1.187 ± 0.041
1.553ProPro: 1.553 ± 0.047
1.6ProGln: 1.6 ± 0.042
2.106ProArg: 2.106 ± 0.05
2.003ProSer: 2.003 ± 0.049
2.096ProThr: 2.096 ± 0.054
4.217ProVal: 4.217 ± 0.068
0.555ProTrp: 0.555 ± 0.027
1.484ProTyr: 1.484 ± 0.034
0.0ProXaa: 0.0 ± 0.0
Gln
4.353GlnAla: 4.353 ± 0.077
0.432GlnCys: 0.432 ± 0.02
1.856GlnAsp: 1.856 ± 0.043
2.348GlnGlu: 2.348 ± 0.058
1.266GlnPhe: 1.266 ± 0.034
2.607GlnGly: 2.607 ± 0.058
0.557GlnHis: 0.557 ± 0.023
2.024GlnIle: 2.024 ± 0.047
1.899GlnLys: 1.899 ± 0.048
4.231GlnLeu: 4.231 ± 0.069
1.143GlnMet: 1.143 ± 0.036
1.381GlnAsn: 1.381 ± 0.036
1.813GlnPro: 1.813 ± 0.044
1.855GlnGln: 1.855 ± 0.048
2.051GlnArg: 2.051 ± 0.045
1.849GlnSer: 1.849 ± 0.047
2.07GlnThr: 2.07 ± 0.044
2.706GlnVal: 2.706 ± 0.054
0.423GlnTrp: 0.423 ± 0.024
1.411GlnTyr: 1.411 ± 0.037
0.0GlnXaa: 0.0 ± 0.0
Arg
5.467ArgAla: 5.467 ± 0.076
1.111ArgCys: 1.111 ± 0.035
2.886ArgAsp: 2.886 ± 0.054
3.966ArgGlu: 3.966 ± 0.064
2.468ArgPhe: 2.468 ± 0.057
3.566ArgGly: 3.566 ± 0.066
1.129ArgHis: 1.129 ± 0.033
3.325ArgIle: 3.325 ± 0.058
2.48ArgLys: 2.48 ± 0.051
6.591ArgLeu: 6.591 ± 0.106
1.805ArgMet: 1.805 ± 0.043
1.682ArgAsn: 1.682 ± 0.042
2.625ArgPro: 2.625 ± 0.055
3.061ArgGln: 3.061 ± 0.063
4.795ArgArg: 4.795 ± 0.085
2.708ArgSer: 2.708 ± 0.058
3.153ArgThr: 3.153 ± 0.057
4.084ArgVal: 4.084 ± 0.061
0.874ArgTrp: 0.874 ± 0.029
2.322ArgTyr: 2.322 ± 0.047
0.0ArgXaa: 0.0 ± 0.0
Ser
5.19SerAla: 5.19 ± 0.095
0.822SerCys: 0.822 ± 0.031
2.567SerAsp: 2.567 ± 0.052
2.879SerGlu: 2.879 ± 0.052
2.229SerPhe: 2.229 ± 0.044
4.858SerGly: 4.858 ± 0.074
0.979SerHis: 0.979 ± 0.031
2.786SerIle: 2.786 ± 0.052
1.71SerLys: 1.71 ± 0.048
5.486SerLeu: 5.486 ± 0.074
1.426SerMet: 1.426 ± 0.038
1.372SerAsn: 1.372 ± 0.04
2.272SerPro: 2.272 ± 0.05
1.84SerGln: 1.84 ± 0.048
3.136SerArg: 3.136 ± 0.052
2.916SerSer: 2.916 ± 0.066
2.559SerThr: 2.559 ± 0.05
3.89SerVal: 3.89 ± 0.065
0.711SerTrp: 0.711 ± 0.028
1.746SerTyr: 1.746 ± 0.043
0.0SerXaa: 0.0 ± 0.0
Thr
6.013ThrAla: 6.013 ± 0.086
0.689ThrCys: 0.689 ± 0.027
3.02ThrAsp: 3.02 ± 0.058
3.166ThrGlu: 3.166 ± 0.063
1.937ThrPhe: 1.937 ± 0.043
5.431ThrGly: 5.431 ± 0.086
0.922ThrHis: 0.922 ± 0.03
2.893ThrIle: 2.893 ± 0.053
1.677ThrLys: 1.677 ± 0.041
6.208ThrLeu: 6.208 ± 0.08
1.352ThrMet: 1.352 ± 0.042
1.44ThrAsn: 1.44 ± 0.045
2.94ThrPro: 2.94 ± 0.059
1.616ThrGln: 1.616 ± 0.039
2.869ThrArg: 2.869 ± 0.057
2.523ThrSer: 2.523 ± 0.054
2.667ThrThr: 2.667 ± 0.059
5.153ThrVal: 5.153 ± 0.084
0.601ThrTrp: 0.601 ± 0.027
1.579ThrTyr: 1.579 ± 0.038
0.0ThrXaa: 0.0 ± 0.0
Val
6.238ValAla: 6.238 ± 0.092
1.518ValCys: 1.518 ± 0.039
3.936ValAsp: 3.936 ± 0.063
4.419ValGlu: 4.419 ± 0.071
2.973ValPhe: 2.973 ± 0.056
4.677ValGly: 4.677 ± 0.075
1.145ValHis: 1.145 ± 0.038
3.751ValIle: 3.751 ± 0.062
2.632ValLys: 2.632 ± 0.06
8.45ValLeu: 8.45 ± 0.111
1.924ValMet: 1.924 ± 0.048
2.28ValAsn: 2.28 ± 0.053
3.28ValPro: 3.28 ± 0.063
2.669ValGln: 2.669 ± 0.053
4.393ValArg: 4.393 ± 0.073
4.328ValSer: 4.328 ± 0.067
4.496ValThr: 4.496 ± 0.075
5.277ValVal: 5.277 ± 0.091
1.002ValTrp: 1.002 ± 0.038
2.523ValTyr: 2.523 ± 0.055
0.0ValXaa: 0.0 ± 0.0
Trp
1.199TrpAla: 1.199 ± 0.04
0.235TrpCys: 0.235 ± 0.015
0.646TrpAsp: 0.646 ± 0.026
0.747TrpGlu: 0.747 ± 0.03
0.5TrpPhe: 0.5 ± 0.022
1.013TrpGly: 1.013 ± 0.035
0.248TrpHis: 0.248 ± 0.017
0.524TrpIle: 0.524 ± 0.023
0.533TrpLys: 0.533 ± 0.023
1.548TrpLeu: 1.548 ± 0.041
0.401TrpMet: 0.401 ± 0.021
0.542TrpAsn: 0.542 ± 0.027
0.533TrpPro: 0.533 ± 0.023
0.785TrpGln: 0.785 ± 0.029
0.727TrpArg: 0.727 ± 0.031
0.518TrpSer: 0.518 ± 0.024
0.554TrpThr: 0.554 ± 0.025
0.783TrpVal: 0.783 ± 0.033
0.186TrpTrp: 0.186 ± 0.015
0.497TrpTyr: 0.497 ± 0.024
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.149TyrAla: 3.149 ± 0.052
0.584TyrCys: 0.584 ± 0.024
2.101TyrAsp: 2.101 ± 0.057
2.075TyrGlu: 2.075 ± 0.05
1.457TyrPhe: 1.457 ± 0.037
2.729TyrGly: 2.729 ± 0.048
0.684TyrHis: 0.684 ± 0.026
1.723TyrIle: 1.723 ± 0.039
1.366TyrLys: 1.366 ± 0.04
3.634TyrLeu: 3.634 ± 0.058
0.801TyrMet: 0.801 ± 0.029
1.296TyrAsn: 1.296 ± 0.036
1.486TyrPro: 1.486 ± 0.039
1.262TyrGln: 1.262 ± 0.04
2.165TyrArg: 2.165 ± 0.053
1.863TyrSer: 1.863 ± 0.044
2.275TyrThr: 2.275 ± 0.052
2.068TyrVal: 2.068 ± 0.051
0.421TyrTrp: 0.421 ± 0.022
1.45TyrTyr: 1.45 ± 0.045
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3048 proteins (991804 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski