Xylanimonas oleitrophica
Average proteome isoelectric point is 6.16
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3108 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2W5WXY7|A0A2W5WXY7_9MICO Uncharacterized N-acetyltransferase DNL40_02185 OS=Xylanimonas oleitrophica OX=2607479 GN=DNL40_02185 PE=3 SV=1
MM1 pKa = 7.43 IRR3 pKa = 11.84 RR4 pKa = 11.84 HH5 pKa = 5.3 SAAKK9 pKa = 10.28 GLALAAALGLGLAACSTSAEE29 pKa = 4.22 GSGGEE34 pKa = 4.38 GSDD37 pKa = 3.33 SAEE40 pKa = 3.83 PLVIGTILPITGTLAFLGPPEE61 pKa = 4.03 VAGVGLAVDD70 pKa = 5.78 DD71 pKa = 4.17 INEE74 pKa = 4.31 AGGVLGNDD82 pKa = 3.83 VSVEE86 pKa = 3.86 WGDD89 pKa = 4.39 SGDD92 pKa = 3.79 TTDD95 pKa = 4.95 LSVAGGTATDD105 pKa = 5.24 LIGKK109 pKa = 8.41 GVSAVVGAASSGVSLAVVDD128 pKa = 4.01 QFEE131 pKa = 4.48 EE132 pKa = 4.36 AGVMQISPANTATDD146 pKa = 3.94 LSGYY150 pKa = 10.78 GDD152 pKa = 4.22 FFARR156 pKa = 11.84 TAPPDD161 pKa = 4.04 TVQGAALGSQILDD174 pKa = 3.87 DD175 pKa = 3.5 GHH177 pKa = 8.15 LRR179 pKa = 11.84 VGFLVQNEE187 pKa = 4.41 AYY189 pKa = 10.61 GNGLRR194 pKa = 11.84 DD195 pKa = 3.76 NVQKK199 pKa = 10.83 AIEE202 pKa = 4.43 SGGGEE207 pKa = 4.02 AVYY210 pKa = 10.24 GATGAGQEE218 pKa = 4.22 FAPGEE223 pKa = 4.51 TNFGAQVTALLATQPDD239 pKa = 4.88 AIAIIAFEE247 pKa = 4.15 EE248 pKa = 4.32 TVAIINEE255 pKa = 4.31 LVAQGWSFSGTTYY268 pKa = 10.59 FCDD271 pKa = 4.05 GNLSNYY277 pKa = 9.6 GDD279 pKa = 3.62 QFDD282 pKa = 4.38 AGTLAGVKK290 pKa = 8.92 GTLPGAQADD299 pKa = 3.98 EE300 pKa = 4.63 EE301 pKa = 4.37 FQQRR305 pKa = 11.84 LNDD308 pKa = 3.49 WYY310 pKa = 11.24 SEE312 pKa = 4.23 NEE314 pKa = 4.11 SGTLNDD320 pKa = 3.14 YY321 pKa = 11.13 SYY323 pKa = 11.51 AAEE326 pKa = 4.7 SYY328 pKa = 10.76 DD329 pKa = 3.61 ATILAALAAVRR340 pKa = 11.84 GGATDD345 pKa = 3.68 GQTIADD351 pKa = 4.09 NIKK354 pKa = 10.22 AVSGAEE360 pKa = 3.88 GGTEE364 pKa = 3.58 VTTFAEE370 pKa = 4.7 GVEE373 pKa = 3.95 ALEE376 pKa = 4.75 NGDD379 pKa = 3.92 EE380 pKa = 3.72 IHH382 pKa = 6.42 YY383 pKa = 10.0 VGKK386 pKa = 10.72 AGIGPLNEE394 pKa = 4.58 SNDD397 pKa = 3.5 PSSAFIGIYY406 pKa = 10.16 EE407 pKa = 4.2 YY408 pKa = 11.52 GDD410 pKa = 4.15 DD411 pKa = 3.61 NTYY414 pKa = 9.45 TYY416 pKa = 11.0 SRR418 pKa = 11.84 SVEE421 pKa = 4.11 GKK423 pKa = 9.68 AA424 pKa = 4.1
Molecular weight: 42.85 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.741
IPC2_protein 3.757
IPC_protein 3.757
Toseland 3.554
ProMoST 3.897
Dawson 3.732
Bjellqvist 3.884
Wikipedia 3.643
Rodwell 3.579
Grimsley 3.452
Solomon 3.732
Lehninger 3.681
Nozaki 3.846
DTASelect 4.05
Thurlkill 3.592
EMBOSS 3.656
Sillero 3.872
Patrickios 1.85
IPC_peptide 3.732
IPC2_peptide 3.859
IPC2.peptide.svr19 3.794
Protein with the highest isoelectric point:
>tr|A0A2W5YCU3|A0A2W5YCU3_9MICO Alpha/beta hydrolase OS=Xylanimonas oleitrophica OX=2607479 GN=DNL40_13520 PE=4 SV=1
MM1 pKa = 7.4 GSVIKK6 pKa = 10.42 KK7 pKa = 8.47 RR8 pKa = 11.84 RR9 pKa = 11.84 KK10 pKa = 9.22 RR11 pKa = 11.84 MAKK14 pKa = 9.41 KK15 pKa = 9.87 KK16 pKa = 9.7 HH17 pKa = 5.84 RR18 pKa = 11.84 KK19 pKa = 8.56 LLRR22 pKa = 11.84 KK23 pKa = 7.78 TRR25 pKa = 11.84 HH26 pKa = 3.65 QRR28 pKa = 11.84 RR29 pKa = 11.84 NKK31 pKa = 9.78 KK32 pKa = 9.85
Molecular weight: 4.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.493
IPC2_protein 11.14
IPC_protein 12.691
Toseland 12.866
ProMoST 13.349
Dawson 12.866
Bjellqvist 12.852
Wikipedia 13.334
Rodwell 12.735
Grimsley 12.91
Solomon 13.349
Lehninger 13.261
Nozaki 12.866
DTASelect 12.852
Thurlkill 12.866
EMBOSS 13.364
Sillero 12.866
Patrickios 12.457
IPC_peptide 13.349
IPC2_peptide 12.34
IPC2.peptide.svr19 9.071
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3108
0
3108
1032422
30
4136
332.2
35.16
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
14.699 ± 0.075
0.521 ± 0.012
6.126 ± 0.044
5.687 ± 0.046
2.453 ± 0.029
9.648 ± 0.045
2.105 ± 0.027
2.635 ± 0.036
1.486 ± 0.03
10.154 ± 0.06
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.578 ± 0.019
1.433 ± 0.026
6.106 ± 0.039
2.859 ± 0.025
7.879 ± 0.058
4.806 ± 0.029
6.207 ± 0.049
10.317 ± 0.053
1.517 ± 0.022
1.784 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here