Sphingomonadales bacterium

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; unclassified Sphingomonadales

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4688 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A4Q5RS48|A0A4Q5RS48_9SPHN Uncharacterized protein OS=Sphingomonadales bacterium OX=1978525 GN=EOP62_20475 PE=4 SV=1
MM1 pKa = 7.73KK2 pKa = 10.24YY3 pKa = 10.02KK4 pKa = 10.81DD5 pKa = 3.42SGAVAGGEE13 pKa = 3.71ARR15 pKa = 11.84MITDD19 pKa = 3.24TLFRR23 pKa = 11.84VGGNDD28 pKa = 3.33EE29 pKa = 4.7GPGLTSDD36 pKa = 3.91VVRR39 pKa = 11.84TAGGPAYY46 pKa = 10.88YY47 pKa = 10.55NIAEE51 pKa = 4.44EE52 pKa = 4.43NGSPTSTVFGTLSHH66 pKa = 6.83SGLGYY71 pKa = 9.94QALGVDD77 pKa = 4.81FAGLSSTNVGPGAAFRR93 pKa = 11.84GIYY96 pKa = 8.03GTLHH100 pKa = 6.45LNSDD104 pKa = 3.65GSFGYY109 pKa = 11.1ALDD112 pKa = 3.97NTDD115 pKa = 4.56ADD117 pKa = 4.42TNQIAAGEE125 pKa = 4.34VTDD128 pKa = 4.32EE129 pKa = 4.32RR130 pKa = 11.84FTYY133 pKa = 9.7TYY135 pKa = 10.6QLNGQIFTDD144 pKa = 3.29KK145 pKa = 10.93VVFHH149 pKa = 6.3ITGVDD154 pKa = 3.2EE155 pKa = 4.96VGQQNHH161 pKa = 5.66YY162 pKa = 11.16LNTQMVRR169 pKa = 11.84TGDD172 pKa = 3.4MTIGANEE179 pKa = 4.01RR180 pKa = 11.84YY181 pKa = 9.93VFNTDD186 pKa = 3.12KK187 pKa = 11.3GISTASDD194 pKa = 3.08TTLDD198 pKa = 3.64VIHH201 pKa = 7.05NYY203 pKa = 10.26GSVRR207 pKa = 11.84VGGVSANGILAMTFDD222 pKa = 4.33PSQYY226 pKa = 10.75SEE228 pKa = 4.75GGSQLINDD236 pKa = 4.0GRR238 pKa = 11.84ITAISSLPSPANTGNNANAVSVSGITQPVTTNNGVIQAINTVTGAVFGAGALAIGAGNVINTGLVEE304 pKa = 4.63SYY306 pKa = 9.33STDD309 pKa = 3.08AGAGAINLGLYY320 pKa = 10.45SPTLINSGVIRR331 pKa = 11.84ASGGPRR337 pKa = 11.84ANPIEE342 pKa = 4.16ISAITAAGTGVHH354 pKa = 5.71VEE356 pKa = 3.85NSGTIEE362 pKa = 3.79AWEE365 pKa = 4.81DD366 pKa = 3.32NAGTQSIAFRR376 pKa = 11.84IFLNNTNVYY385 pKa = 9.62QGGIIANSGRR395 pKa = 11.84IVADD399 pKa = 3.18QAINALEE406 pKa = 4.72GIGGSLHH413 pKa = 6.51VINTGYY419 pKa = 10.09IQGDD423 pKa = 3.89YY424 pKa = 10.98VRR426 pKa = 11.84DD427 pKa = 3.96GVGHH431 pKa = 6.9DD432 pKa = 3.64VVTNAANGVWRR443 pKa = 11.84GNLGFATNADD453 pKa = 3.95LVRR456 pKa = 11.84NAGTVVGTISLGGGADD472 pKa = 3.21MFDD475 pKa = 3.48ARR477 pKa = 11.84TGGIVNGTVNGGAGNDD493 pKa = 3.52VLLGGIHH500 pKa = 7.59ADD502 pKa = 3.63TLIGGDD508 pKa = 3.58GADD511 pKa = 3.57ALFGGGGADD520 pKa = 3.55QLTGGAGADD529 pKa = 3.42LFIYY533 pKa = 10.33AVGDD537 pKa = 4.13SIVGARR543 pKa = 11.84DD544 pKa = 3.53TITDD548 pKa = 4.21FQTGQDD554 pKa = 3.85RR555 pKa = 11.84IDD557 pKa = 3.72LSRR560 pKa = 11.84LGVTSYY566 pKa = 11.55AITTEE571 pKa = 3.72GAYY574 pKa = 9.05TVLSGMGAGGAFNIAASGTIAASDD598 pKa = 4.8LIFAAQTSVMRR609 pKa = 11.84GGAGDD614 pKa = 4.54DD615 pKa = 3.69LLIAAMIGVTMDD627 pKa = 3.52GDD629 pKa = 3.58NGNDD633 pKa = 3.39VLVGSSGNDD642 pKa = 3.36VLDD645 pKa = 3.89GWNGADD651 pKa = 3.54TMFGGDD657 pKa = 3.5GDD659 pKa = 3.94DD660 pKa = 3.84TYY662 pKa = 12.01VVDD665 pKa = 3.85TDD667 pKa = 3.43GDD669 pKa = 3.84RR670 pKa = 11.84TIEE673 pKa = 4.12ADD675 pKa = 3.46GGGHH679 pKa = 7.12DD680 pKa = 4.83SVFSYY685 pKa = 10.77IDD687 pKa = 3.32YY688 pKa = 10.98SLQFWVEE695 pKa = 4.16DD696 pKa = 3.55GTLLGGSSSTIGLLGNRR713 pKa = 11.84LDD715 pKa = 3.54NTLRR719 pKa = 11.84GNAGSNRR726 pKa = 11.84ILGMDD731 pKa = 3.9GNDD734 pKa = 3.5VIIGGGGADD743 pKa = 3.89EE744 pKa = 4.82LFGGAGADD752 pKa = 3.14RR753 pKa = 11.84FVYY756 pKa = 10.15EE757 pKa = 4.28VASDD761 pKa = 3.51STAAAFDD768 pKa = 3.63WLKK771 pKa = 10.36MFEE774 pKa = 4.57HH775 pKa = 7.18GIDD778 pKa = 4.07SIDD781 pKa = 3.56LRR783 pKa = 11.84AIPVTTFNFEE793 pKa = 4.02PYY795 pKa = 9.65KK796 pKa = 10.89NSWQYY801 pKa = 9.82TEE803 pKa = 4.03WTTVTIGTTSGATMVMRR820 pKa = 11.84VDD822 pKa = 4.25GTATMADD829 pKa = 3.74FLVTARR835 pKa = 11.84GPTPNDD841 pKa = 4.54DD842 pKa = 3.96ILTGTNGVDD851 pKa = 3.99VIHH854 pKa = 6.74GLAGNDD860 pKa = 4.08TIDD863 pKa = 3.74GLLGADD869 pKa = 3.53QLYY872 pKa = 10.89GDD874 pKa = 4.82EE875 pKa = 4.85GNDD878 pKa = 3.25LFVFSAVQFGNTPDD892 pKa = 3.96LGLIDD897 pKa = 5.12GGADD901 pKa = 3.55YY902 pKa = 8.77DD903 pKa = 4.3TVDD906 pKa = 4.67LRR908 pKa = 11.84NISPAFVGTVALPGGGFSTALFVGSQRR935 pKa = 11.84YY936 pKa = 7.93QLTGVEE942 pKa = 5.17LILFGDD948 pKa = 4.73GEE950 pKa = 4.19NSLIGRR956 pKa = 11.84VSGSAPLEE964 pKa = 3.73IRR966 pKa = 11.84AGGGADD972 pKa = 4.23DD973 pKa = 4.31LTGSGGDD980 pKa = 3.28RR981 pKa = 11.84LYY983 pKa = 11.57GEE985 pKa = 5.22AGDD988 pKa = 4.4DD989 pKa = 3.4RR990 pKa = 11.84FFISGGFGVQPVSGVVDD1007 pKa = 4.28GGTGIDD1013 pKa = 3.44TLRR1016 pKa = 11.84LNNAFTVDD1024 pKa = 4.21LAAGTAVAGSYY1035 pKa = 9.09TYY1037 pKa = 10.13QVSGIEE1043 pKa = 4.2IVSAMTDD1050 pKa = 3.22SVVRR1054 pKa = 11.84GDD1056 pKa = 3.37AGANIFNVNPLFDD1069 pKa = 3.78STSASVFFDD1078 pKa = 2.99GRR1080 pKa = 11.84EE1081 pKa = 4.03GDD1083 pKa = 3.99DD1084 pKa = 3.73SLTGSMGNDD1093 pKa = 3.24QLIGGAGNDD1102 pKa = 3.92TLVGGAGADD1111 pKa = 3.35AMTGGSGNDD1120 pKa = 3.71LYY1122 pKa = 11.65SVDD1125 pKa = 3.61NAGDD1129 pKa = 3.67VVTEE1133 pKa = 4.07LLGEE1137 pKa = 4.68GYY1139 pKa = 10.7DD1140 pKa = 3.68EE1141 pKa = 4.56VSASISYY1148 pKa = 10.6VLTANVEE1155 pKa = 4.07GLRR1158 pKa = 11.84LVNGSGALNGTGNALDD1174 pKa = 4.1NLITGNDD1181 pKa = 3.59GANRR1185 pKa = 11.84LDD1187 pKa = 4.19GGDD1190 pKa = 3.88GNDD1193 pKa = 4.23ILWSSDD1199 pKa = 2.93GTSDD1203 pKa = 5.23DD1204 pKa = 3.64VGLEE1208 pKa = 3.75HH1209 pKa = 7.52DD1210 pKa = 4.29QLFGGAGNDD1219 pKa = 3.39ALAVGVGDD1227 pKa = 4.87DD1228 pKa = 3.93ADD1230 pKa = 4.17GGSGVDD1236 pKa = 3.46TLRR1239 pKa = 11.84LSLAGFSWGVTLDD1252 pKa = 3.39MSGFTSGGAFNIFGGIISEE1271 pKa = 4.38IEE1273 pKa = 4.04VIDD1276 pKa = 4.43YY1277 pKa = 10.77IVGSAGNDD1285 pKa = 3.29KK1286 pKa = 9.37ITLGGHH1292 pKa = 5.53TYY1294 pKa = 10.91GVTVKK1299 pKa = 10.42SGAGNDD1305 pKa = 3.86TLLSGAGADD1314 pKa = 3.69HH1315 pKa = 7.4LYY1317 pKa = 11.07GGLGDD1322 pKa = 4.67DD1323 pKa = 4.1VFVVDD1328 pKa = 4.59TGDD1331 pKa = 3.92DD1332 pKa = 3.79LVFEE1336 pKa = 4.44NAGEE1340 pKa = 4.27GFDD1343 pKa = 3.3IVYY1346 pKa = 10.45AAGSYY1351 pKa = 10.83ALVAGSHH1358 pKa = 5.65VEE1360 pKa = 3.92LLTTLSVDD1368 pKa = 3.61GAVTLVGNEE1377 pKa = 3.85QANTILGNAGANYY1390 pKa = 10.11LAGGAGADD1398 pKa = 3.51TLAGNGGNDD1407 pKa = 3.26VMFGEE1412 pKa = 5.75DD1413 pKa = 3.97GDD1415 pKa = 5.24DD1416 pKa = 5.76LIGDD1420 pKa = 4.27DD1421 pKa = 5.43AGDD1424 pKa = 4.27DD1425 pKa = 3.95YY1426 pKa = 12.22AEE1428 pKa = 4.66GGAGNDD1434 pKa = 3.75YY1435 pKa = 10.8IYY1437 pKa = 10.88LGAGNDD1443 pKa = 3.52RR1444 pKa = 11.84LYY1446 pKa = 11.41GGAGNDD1452 pKa = 3.95LLFGQDD1458 pKa = 3.65GDD1460 pKa = 4.19DD1461 pKa = 3.69TLGDD1465 pKa = 3.72GDD1467 pKa = 4.98GDD1469 pKa = 4.11DD1470 pKa = 4.37MLDD1473 pKa = 3.53GGEE1476 pKa = 4.32GNDD1479 pKa = 3.59YY1480 pKa = 10.88LYY1482 pKa = 11.16AGIGNDD1488 pKa = 3.11RR1489 pKa = 11.84LYY1491 pKa = 11.36GGGGDD1496 pKa = 3.91DD1497 pKa = 5.18QMFGQDD1503 pKa = 3.88GDD1505 pKa = 4.78DD1506 pKa = 3.34IMGGGGGADD1515 pKa = 3.53YY1516 pKa = 10.72MEE1518 pKa = 5.4AGAGKK1523 pKa = 10.04DD1524 pKa = 3.34FAYY1527 pKa = 10.4GGDD1530 pKa = 3.81GNDD1533 pKa = 3.22VVLGEE1538 pKa = 4.85AGDD1541 pKa = 3.81DD1542 pKa = 3.52QLYY1545 pKa = 11.03GEE1547 pKa = 5.31GGDD1550 pKa = 4.91DD1551 pKa = 3.52ILIGGTGNDD1560 pKa = 3.3QLFGGEE1566 pKa = 4.22GADD1569 pKa = 3.34ILGDD1573 pKa = 4.01AEE1575 pKa = 5.1GDD1577 pKa = 3.75DD1578 pKa = 4.08YY1579 pKa = 11.5MQGGGGADD1587 pKa = 3.52YY1588 pKa = 10.66LYY1590 pKa = 10.93AGAGRR1595 pKa = 11.84DD1596 pKa = 3.51TLYY1599 pKa = 11.38GDD1601 pKa = 4.43GGNDD1605 pKa = 2.86ILFGQDD1611 pKa = 3.3GDD1613 pKa = 4.84DD1614 pKa = 5.06LLGDD1618 pKa = 4.22DD1619 pKa = 5.11EE1620 pKa = 7.2GDD1622 pKa = 3.78DD1623 pKa = 4.38VLDD1626 pKa = 4.28GGNGNDD1632 pKa = 3.79YY1633 pKa = 10.11MFAGAGADD1641 pKa = 3.44RR1642 pKa = 11.84LYY1644 pKa = 11.38GGAGDD1649 pKa = 5.57DD1650 pKa = 5.28LMFGQDD1656 pKa = 4.55GDD1658 pKa = 4.64DD1659 pKa = 3.77EE1660 pKa = 4.48MAGGDD1665 pKa = 4.13GADD1668 pKa = 4.01MLDD1671 pKa = 3.68GGAGADD1677 pKa = 3.6YY1678 pKa = 10.44LYY1680 pKa = 11.02SGTGNDD1686 pKa = 3.45RR1687 pKa = 11.84LYY1689 pKa = 11.34GGDD1692 pKa = 4.06GNDD1695 pKa = 4.14LMFGQEE1701 pKa = 4.53GDD1703 pKa = 4.06DD1704 pKa = 3.94EE1705 pKa = 4.71LAGGAGADD1713 pKa = 3.98YY1714 pKa = 11.08IEE1716 pKa = 5.44GGDD1719 pKa = 4.26GNDD1722 pKa = 4.7LIYY1725 pKa = 11.06GGAGTDD1731 pKa = 3.42MLYY1734 pKa = 11.08GGAGADD1740 pKa = 3.21AFAFMAAPGAANLQTIGDD1758 pKa = 4.0FTVGQDD1764 pKa = 3.15RR1765 pKa = 11.84LLLSGAVFAGLAPGALAAGAFVTGTAAADD1794 pKa = 3.28ASDD1797 pKa = 4.48RR1798 pKa = 11.84ILYY1801 pKa = 9.22NAATGALWFDD1811 pKa = 3.79ADD1813 pKa = 3.76GTGAIAAVQFGMLSPGLALSATDD1836 pKa = 3.6FAVII1840 pKa = 4.32

Molecular weight:
184.91 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A4Q5RYX5|A0A4Q5RYX5_9SPHN Uncharacterized protein OS=Sphingomonadales bacterium OX=1978525 GN=EOP62_08285 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.58GFRR19 pKa = 11.84SRR21 pKa = 11.84MATPGGRR28 pKa = 11.84KK29 pKa = 9.09VIHH32 pKa = 6.46ARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84SKK41 pKa = 11.12LSAA44 pKa = 3.81

Molecular weight:
5.06 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4688

0

4688

1519867

22

4969

324.2

35.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.074 ± 0.047

0.757 ± 0.011

5.838 ± 0.033

5.454 ± 0.038

3.661 ± 0.022

8.965 ± 0.045

1.939 ± 0.019

5.181 ± 0.025

3.159 ± 0.027

9.686 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.418 ± 0.02

2.722 ± 0.031

5.227 ± 0.033

3.042 ± 0.02

7.046 ± 0.044

5.474 ± 0.027

5.48 ± 0.052

7.131 ± 0.029

1.459 ± 0.018

2.289 ± 0.018

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski