Sphingomonadales bacterium
Average proteome isoelectric point is 6.49
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4688 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4Q5RS48|A0A4Q5RS48_9SPHN Uncharacterized protein OS=Sphingomonadales bacterium OX=1978525 GN=EOP62_20475 PE=4 SV=1
MM1 pKa = 7.73 KK2 pKa = 10.24 YY3 pKa = 10.02 KK4 pKa = 10.81 DD5 pKa = 3.42 SGAVAGGEE13 pKa = 3.71 ARR15 pKa = 11.84 MITDD19 pKa = 3.24 TLFRR23 pKa = 11.84 VGGNDD28 pKa = 3.33 EE29 pKa = 4.7 GPGLTSDD36 pKa = 3.91 VVRR39 pKa = 11.84 TAGGPAYY46 pKa = 10.88 YY47 pKa = 10.55 NIAEE51 pKa = 4.44 EE52 pKa = 4.43 NGSPTSTVFGTLSHH66 pKa = 6.83 SGLGYY71 pKa = 9.94 QALGVDD77 pKa = 4.81 FAGLSSTNVGPGAAFRR93 pKa = 11.84 GIYY96 pKa = 8.03 GTLHH100 pKa = 6.45 LNSDD104 pKa = 3.65 GSFGYY109 pKa = 11.1 ALDD112 pKa = 3.97 NTDD115 pKa = 4.56 ADD117 pKa = 4.42 TNQIAAGEE125 pKa = 4.34 VTDD128 pKa = 4.32 EE129 pKa = 4.32 RR130 pKa = 11.84 FTYY133 pKa = 9.7 TYY135 pKa = 10.6 QLNGQIFTDD144 pKa = 3.29 KK145 pKa = 10.93 VVFHH149 pKa = 6.3 ITGVDD154 pKa = 3.2 EE155 pKa = 4.96 VGQQNHH161 pKa = 5.66 YY162 pKa = 11.16 LNTQMVRR169 pKa = 11.84 TGDD172 pKa = 3.4 MTIGANEE179 pKa = 4.01 RR180 pKa = 11.84 YY181 pKa = 9.93 VFNTDD186 pKa = 3.12 KK187 pKa = 11.3 GISTASDD194 pKa = 3.08 TTLDD198 pKa = 3.64 VIHH201 pKa = 7.05 NYY203 pKa = 10.26 GSVRR207 pKa = 11.84 VGGVSANGILAMTFDD222 pKa = 4.33 PSQYY226 pKa = 10.75 SEE228 pKa = 4.75 GGSQLINDD236 pKa = 4.0 GRR238 pKa = 11.84 ITAISSLPSPANTGNNANAVSVSGITQPVTTNNGVIQAINTVTGAVFGAGALAIGAGNVINTGLVEE304 pKa = 4.63 SYY306 pKa = 9.33 STDD309 pKa = 3.08 AGAGAINLGLYY320 pKa = 10.45 SPTLINSGVIRR331 pKa = 11.84 ASGGPRR337 pKa = 11.84 ANPIEE342 pKa = 4.16 ISAITAAGTGVHH354 pKa = 5.71 VEE356 pKa = 3.85 NSGTIEE362 pKa = 3.79 AWEE365 pKa = 4.81 DD366 pKa = 3.32 NAGTQSIAFRR376 pKa = 11.84 IFLNNTNVYY385 pKa = 9.62 QGGIIANSGRR395 pKa = 11.84 IVADD399 pKa = 3.18 QAINALEE406 pKa = 4.72 GIGGSLHH413 pKa = 6.51 VINTGYY419 pKa = 10.09 IQGDD423 pKa = 3.89 YY424 pKa = 10.98 VRR426 pKa = 11.84 DD427 pKa = 3.96 GVGHH431 pKa = 6.9 DD432 pKa = 3.64 VVTNAANGVWRR443 pKa = 11.84 GNLGFATNADD453 pKa = 3.95 LVRR456 pKa = 11.84 NAGTVVGTISLGGGADD472 pKa = 3.21 MFDD475 pKa = 3.48 ARR477 pKa = 11.84 TGGIVNGTVNGGAGNDD493 pKa = 3.52 VLLGGIHH500 pKa = 7.59 ADD502 pKa = 3.63 TLIGGDD508 pKa = 3.58 GADD511 pKa = 3.57 ALFGGGGADD520 pKa = 3.55 QLTGGAGADD529 pKa = 3.42 LFIYY533 pKa = 10.33 AVGDD537 pKa = 4.13 SIVGARR543 pKa = 11.84 DD544 pKa = 3.53 TITDD548 pKa = 4.21 FQTGQDD554 pKa = 3.85 RR555 pKa = 11.84 IDD557 pKa = 3.72 LSRR560 pKa = 11.84 LGVTSYY566 pKa = 11.55 AITTEE571 pKa = 3.72 GAYY574 pKa = 9.05 TVLSGMGAGGAFNIAASGTIAASDD598 pKa = 4.8 LIFAAQTSVMRR609 pKa = 11.84 GGAGDD614 pKa = 4.54 DD615 pKa = 3.69 LLIAAMIGVTMDD627 pKa = 3.52 GDD629 pKa = 3.58 NGNDD633 pKa = 3.39 VLVGSSGNDD642 pKa = 3.36 VLDD645 pKa = 3.89 GWNGADD651 pKa = 3.54 TMFGGDD657 pKa = 3.5 GDD659 pKa = 3.94 DD660 pKa = 3.84 TYY662 pKa = 12.01 VVDD665 pKa = 3.85 TDD667 pKa = 3.43 GDD669 pKa = 3.84 RR670 pKa = 11.84 TIEE673 pKa = 4.12 ADD675 pKa = 3.46 GGGHH679 pKa = 7.12 DD680 pKa = 4.83 SVFSYY685 pKa = 10.77 IDD687 pKa = 3.32 YY688 pKa = 10.98 SLQFWVEE695 pKa = 4.16 DD696 pKa = 3.55 GTLLGGSSSTIGLLGNRR713 pKa = 11.84 LDD715 pKa = 3.54 NTLRR719 pKa = 11.84 GNAGSNRR726 pKa = 11.84 ILGMDD731 pKa = 3.9 GNDD734 pKa = 3.5 VIIGGGGADD743 pKa = 3.89 EE744 pKa = 4.82 LFGGAGADD752 pKa = 3.14 RR753 pKa = 11.84 FVYY756 pKa = 10.15 EE757 pKa = 4.28 VASDD761 pKa = 3.51 STAAAFDD768 pKa = 3.63 WLKK771 pKa = 10.36 MFEE774 pKa = 4.57 HH775 pKa = 7.18 GIDD778 pKa = 4.07 SIDD781 pKa = 3.56 LRR783 pKa = 11.84 AIPVTTFNFEE793 pKa = 4.02 PYY795 pKa = 9.65 KK796 pKa = 10.89 NSWQYY801 pKa = 9.82 TEE803 pKa = 4.03 WTTVTIGTTSGATMVMRR820 pKa = 11.84 VDD822 pKa = 4.25 GTATMADD829 pKa = 3.74 FLVTARR835 pKa = 11.84 GPTPNDD841 pKa = 4.54 DD842 pKa = 3.96 ILTGTNGVDD851 pKa = 3.99 VIHH854 pKa = 6.74 GLAGNDD860 pKa = 4.08 TIDD863 pKa = 3.74 GLLGADD869 pKa = 3.53 QLYY872 pKa = 10.89 GDD874 pKa = 4.82 EE875 pKa = 4.85 GNDD878 pKa = 3.25 LFVFSAVQFGNTPDD892 pKa = 3.96 LGLIDD897 pKa = 5.12 GGADD901 pKa = 3.55 YY902 pKa = 8.77 DD903 pKa = 4.3 TVDD906 pKa = 4.67 LRR908 pKa = 11.84 NISPAFVGTVALPGGGFSTALFVGSQRR935 pKa = 11.84 YY936 pKa = 7.93 QLTGVEE942 pKa = 5.17 LILFGDD948 pKa = 4.73 GEE950 pKa = 4.19 NSLIGRR956 pKa = 11.84 VSGSAPLEE964 pKa = 3.73 IRR966 pKa = 11.84 AGGGADD972 pKa = 4.23 DD973 pKa = 4.31 LTGSGGDD980 pKa = 3.28 RR981 pKa = 11.84 LYY983 pKa = 11.57 GEE985 pKa = 5.22 AGDD988 pKa = 4.4 DD989 pKa = 3.4 RR990 pKa = 11.84 FFISGGFGVQPVSGVVDD1007 pKa = 4.28 GGTGIDD1013 pKa = 3.44 TLRR1016 pKa = 11.84 LNNAFTVDD1024 pKa = 4.21 LAAGTAVAGSYY1035 pKa = 9.09 TYY1037 pKa = 10.13 QVSGIEE1043 pKa = 4.2 IVSAMTDD1050 pKa = 3.22 SVVRR1054 pKa = 11.84 GDD1056 pKa = 3.37 AGANIFNVNPLFDD1069 pKa = 3.78 STSASVFFDD1078 pKa = 2.99 GRR1080 pKa = 11.84 EE1081 pKa = 4.03 GDD1083 pKa = 3.99 DD1084 pKa = 3.73 SLTGSMGNDD1093 pKa = 3.24 QLIGGAGNDD1102 pKa = 3.92 TLVGGAGADD1111 pKa = 3.35 AMTGGSGNDD1120 pKa = 3.71 LYY1122 pKa = 11.65 SVDD1125 pKa = 3.61 NAGDD1129 pKa = 3.67 VVTEE1133 pKa = 4.07 LLGEE1137 pKa = 4.68 GYY1139 pKa = 10.7 DD1140 pKa = 3.68 EE1141 pKa = 4.56 VSASISYY1148 pKa = 10.6 VLTANVEE1155 pKa = 4.07 GLRR1158 pKa = 11.84 LVNGSGALNGTGNALDD1174 pKa = 4.1 NLITGNDD1181 pKa = 3.59 GANRR1185 pKa = 11.84 LDD1187 pKa = 4.19 GGDD1190 pKa = 3.88 GNDD1193 pKa = 4.23 ILWSSDD1199 pKa = 2.93 GTSDD1203 pKa = 5.23 DD1204 pKa = 3.64 VGLEE1208 pKa = 3.75 HH1209 pKa = 7.52 DD1210 pKa = 4.29 QLFGGAGNDD1219 pKa = 3.39 ALAVGVGDD1227 pKa = 4.87 DD1228 pKa = 3.93 ADD1230 pKa = 4.17 GGSGVDD1236 pKa = 3.46 TLRR1239 pKa = 11.84 LSLAGFSWGVTLDD1252 pKa = 3.39 MSGFTSGGAFNIFGGIISEE1271 pKa = 4.38 IEE1273 pKa = 4.04 VIDD1276 pKa = 4.43 YY1277 pKa = 10.77 IVGSAGNDD1285 pKa = 3.29 KK1286 pKa = 9.37 ITLGGHH1292 pKa = 5.53 TYY1294 pKa = 10.91 GVTVKK1299 pKa = 10.42 SGAGNDD1305 pKa = 3.86 TLLSGAGADD1314 pKa = 3.69 HH1315 pKa = 7.4 LYY1317 pKa = 11.07 GGLGDD1322 pKa = 4.67 DD1323 pKa = 4.1 VFVVDD1328 pKa = 4.59 TGDD1331 pKa = 3.92 DD1332 pKa = 3.79 LVFEE1336 pKa = 4.44 NAGEE1340 pKa = 4.27 GFDD1343 pKa = 3.3 IVYY1346 pKa = 10.45 AAGSYY1351 pKa = 10.83 ALVAGSHH1358 pKa = 5.65 VEE1360 pKa = 3.92 LLTTLSVDD1368 pKa = 3.61 GAVTLVGNEE1377 pKa = 3.85 QANTILGNAGANYY1390 pKa = 10.11 LAGGAGADD1398 pKa = 3.51 TLAGNGGNDD1407 pKa = 3.26 VMFGEE1412 pKa = 5.75 DD1413 pKa = 3.97 GDD1415 pKa = 5.24 DD1416 pKa = 5.76 LIGDD1420 pKa = 4.27 DD1421 pKa = 5.43 AGDD1424 pKa = 4.27 DD1425 pKa = 3.95 YY1426 pKa = 12.22 AEE1428 pKa = 4.66 GGAGNDD1434 pKa = 3.75 YY1435 pKa = 10.8 IYY1437 pKa = 10.88 LGAGNDD1443 pKa = 3.52 RR1444 pKa = 11.84 LYY1446 pKa = 11.41 GGAGNDD1452 pKa = 3.95 LLFGQDD1458 pKa = 3.65 GDD1460 pKa = 4.19 DD1461 pKa = 3.69 TLGDD1465 pKa = 3.72 GDD1467 pKa = 4.98 GDD1469 pKa = 4.11 DD1470 pKa = 4.37 MLDD1473 pKa = 3.53 GGEE1476 pKa = 4.32 GNDD1479 pKa = 3.59 YY1480 pKa = 10.88 LYY1482 pKa = 11.16 AGIGNDD1488 pKa = 3.11 RR1489 pKa = 11.84 LYY1491 pKa = 11.36 GGGGDD1496 pKa = 3.91 DD1497 pKa = 5.18 QMFGQDD1503 pKa = 3.88 GDD1505 pKa = 4.78 DD1506 pKa = 3.34 IMGGGGGADD1515 pKa = 3.53 YY1516 pKa = 10.72 MEE1518 pKa = 5.4 AGAGKK1523 pKa = 10.04 DD1524 pKa = 3.34 FAYY1527 pKa = 10.4 GGDD1530 pKa = 3.81 GNDD1533 pKa = 3.22 VVLGEE1538 pKa = 4.85 AGDD1541 pKa = 3.81 DD1542 pKa = 3.52 QLYY1545 pKa = 11.03 GEE1547 pKa = 5.31 GGDD1550 pKa = 4.91 DD1551 pKa = 3.52 ILIGGTGNDD1560 pKa = 3.3 QLFGGEE1566 pKa = 4.22 GADD1569 pKa = 3.34 ILGDD1573 pKa = 4.01 AEE1575 pKa = 5.1 GDD1577 pKa = 3.75 DD1578 pKa = 4.08 YY1579 pKa = 11.5 MQGGGGADD1587 pKa = 3.52 YY1588 pKa = 10.66 LYY1590 pKa = 10.93 AGAGRR1595 pKa = 11.84 DD1596 pKa = 3.51 TLYY1599 pKa = 11.38 GDD1601 pKa = 4.43 GGNDD1605 pKa = 2.86 ILFGQDD1611 pKa = 3.3 GDD1613 pKa = 4.84 DD1614 pKa = 5.06 LLGDD1618 pKa = 4.22 DD1619 pKa = 5.11 EE1620 pKa = 7.2 GDD1622 pKa = 3.78 DD1623 pKa = 4.38 VLDD1626 pKa = 4.28 GGNGNDD1632 pKa = 3.79 YY1633 pKa = 10.11 MFAGAGADD1641 pKa = 3.44 RR1642 pKa = 11.84 LYY1644 pKa = 11.38 GGAGDD1649 pKa = 5.57 DD1650 pKa = 5.28 LMFGQDD1656 pKa = 4.55 GDD1658 pKa = 4.64 DD1659 pKa = 3.77 EE1660 pKa = 4.48 MAGGDD1665 pKa = 4.13 GADD1668 pKa = 4.01 MLDD1671 pKa = 3.68 GGAGADD1677 pKa = 3.6 YY1678 pKa = 10.44 LYY1680 pKa = 11.02 SGTGNDD1686 pKa = 3.45 RR1687 pKa = 11.84 LYY1689 pKa = 11.34 GGDD1692 pKa = 4.06 GNDD1695 pKa = 4.14 LMFGQEE1701 pKa = 4.53 GDD1703 pKa = 4.06 DD1704 pKa = 3.94 EE1705 pKa = 4.71 LAGGAGADD1713 pKa = 3.98 YY1714 pKa = 11.08 IEE1716 pKa = 5.44 GGDD1719 pKa = 4.26 GNDD1722 pKa = 4.7 LIYY1725 pKa = 11.06 GGAGTDD1731 pKa = 3.42 MLYY1734 pKa = 11.08 GGAGADD1740 pKa = 3.21 AFAFMAAPGAANLQTIGDD1758 pKa = 4.0 FTVGQDD1764 pKa = 3.15 RR1765 pKa = 11.84 LLLSGAVFAGLAPGALAAGAFVTGTAAADD1794 pKa = 3.28 ASDD1797 pKa = 4.48 RR1798 pKa = 11.84 ILYY1801 pKa = 9.22 NAATGALWFDD1811 pKa = 3.79 ADD1813 pKa = 3.76 GTGAIAAVQFGMLSPGLALSATDD1836 pKa = 3.6 FAVII1840 pKa = 4.32
Molecular weight: 184.91 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.74
IPC2_protein 3.465
IPC_protein 3.541
Toseland 3.287
ProMoST 3.719
Dawson 3.554
Bjellqvist 3.706
Wikipedia 3.516
Rodwell 3.35
Grimsley 3.185
Solomon 3.554
Lehninger 3.516
Nozaki 3.656
DTASelect 3.986
Thurlkill 3.35
EMBOSS 3.528
Sillero 3.668
Patrickios 1.074
IPC_peptide 3.541
IPC2_peptide 3.643
IPC2.peptide.svr19 3.66
Protein with the highest isoelectric point:
>tr|A0A4Q5RYX5|A0A4Q5RYX5_9SPHN Uncharacterized protein OS=Sphingomonadales bacterium OX=1978525 GN=EOP62_08285 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNLVRR12 pKa = 11.84 ARR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.58 GFRR19 pKa = 11.84 SRR21 pKa = 11.84 MATPGGRR28 pKa = 11.84 KK29 pKa = 9.09 VIHH32 pKa = 6.46 ARR34 pKa = 11.84 RR35 pKa = 11.84 ARR37 pKa = 11.84 GRR39 pKa = 11.84 SKK41 pKa = 11.12 LSAA44 pKa = 3.81
Molecular weight: 5.06 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 12.998
ProMoST 13.495
Dawson 12.998
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.618
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 12.998
DTASelect 12.998
Thurlkill 12.998
EMBOSS 13.51
Sillero 12.998
Patrickios 12.34
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.193
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4688
0
4688
1519867
22
4969
324.2
35.02
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.074 ± 0.047
0.757 ± 0.011
5.838 ± 0.033
5.454 ± 0.038
3.661 ± 0.022
8.965 ± 0.045
1.939 ± 0.019
5.181 ± 0.025
3.159 ± 0.027
9.686 ± 0.042
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.418 ± 0.02
2.722 ± 0.031
5.227 ± 0.033
3.042 ± 0.02
7.046 ± 0.044
5.474 ± 0.027
5.48 ± 0.052
7.131 ± 0.029
1.459 ± 0.018
2.289 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here