Amino acid dipepetide frequency for Sphingomonadales bacterium

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	18.62AlaAla: 18.62 ± 0.203	0.992AlaCys: 0.992 ± 0.024	7.358AlaAsp: 7.358 ± 0.083	7.718AlaGlu: 7.718 ± 0.099	4.447AlaPhe: 4.447 ± 0.062	11.389AlaGly: 11.389 ± 0.112	2.33AlaHis: 2.33 ± 0.041	6.982AlaIle: 6.982 ± 0.076	4.331AlaLys: 4.331 ± 0.061	13.48AlaLeu: 13.48 ± 0.149	3.848AlaMet: 3.848 ± 0.053	3.39AlaAsn: 3.39 ± 0.055	6.27AlaPro: 6.27 ± 0.084	4.192AlaGln: 4.192 ± 0.058	8.965AlaArg: 8.965 ± 0.101	6.922AlaSer: 6.922 ± 0.07	6.787AlaThr: 6.787 ± 0.099	8.428AlaVal: 8.428 ± 0.093	1.707AlaTrp: 1.707 ± 0.036	2.585AlaTyr: 2.585 ± 0.038	0.0AlaXaa: 0.0 ± 0.0
Cys	0.976CysAla: 0.976 ± 0.029	0.084CysCys: 0.084 ± 0.007	0.481CysAsp: 0.481 ± 0.016	0.386CysGlu: 0.386 ± 0.017	0.312CysPhe: 0.312 ± 0.015	0.783CysGly: 0.783 ± 0.025	0.208CysHis: 0.208 ± 0.012	0.329CysIle: 0.329 ± 0.015	0.197CysLys: 0.197 ± 0.011	0.628CysLeu: 0.628 ± 0.022	0.151CysMet: 0.151 ± 0.011	0.218CysAsn: 0.218 ± 0.011	0.397CysPro: 0.397 ± 0.018	0.194CysGln: 0.194 ± 0.01	0.537CysArg: 0.537 ± 0.019	0.431CysSer: 0.431 ± 0.017	0.42CysThr: 0.42 ± 0.016	0.553CysVal: 0.553 ± 0.02	0.114CysTrp: 0.114 ± 0.009	0.168CysTyr: 0.168 ± 0.01	0.0CysXaa: 0.0 ± 0.0
Asp	7.737AspAla: 7.737 ± 0.077	0.455AspCys: 0.455 ± 0.017	3.228AspAsp: 3.228 ± 0.06	3.231AspGlu: 3.231 ± 0.05	2.197AspPhe: 2.197 ± 0.043	5.33AspGly: 5.33 ± 0.085	1.226AspHis: 1.226 ± 0.03	2.783AspIle: 2.783 ± 0.049	1.806AspLys: 1.806 ± 0.037	5.577AspLeu: 5.577 ± 0.067	1.327AspMet: 1.327 ± 0.027	1.361AspAsn: 1.361 ± 0.036	3.69AspPro: 3.69 ± 0.054	1.732AspGln: 1.732 ± 0.038	4.513AspArg: 4.513 ± 0.061	2.474AspSer: 2.474 ± 0.045	2.831AspThr: 2.831 ± 0.049	4.179AspVal: 4.179 ± 0.055	1.101AspTrp: 1.101 ± 0.032	1.6AspTyr: 1.6 ± 0.036	0.0AspXaa: 0.0 ± 0.0
Glu	7.618GluAla: 7.618 ± 0.095	0.322GluCys: 0.322 ± 0.018	2.68GluAsp: 2.68 ± 0.05	2.8GluGlu: 2.8 ± 0.057	1.65GluPhe: 1.65 ± 0.037	4.547GluGly: 4.547 ± 0.055	1.174GluHis: 1.174 ± 0.029	3.195GluIle: 3.195 ± 0.047	1.984GluLys: 1.984 ± 0.039	5.071GluLeu: 5.071 ± 0.062	1.444GluMet: 1.444 ± 0.033	1.428GluAsn: 1.428 ± 0.035	2.49GluPro: 2.49 ± 0.049	2.013GluGln: 2.013 ± 0.038	4.848GluArg: 4.848 ± 0.07	2.486GluSer: 2.486 ± 0.044	3.208GluThr: 3.208 ± 0.053	3.604GluVal: 3.604 ± 0.044	0.862GluTrp: 0.862 ± 0.026	1.111GluTyr: 1.111 ± 0.029	0.0GluXaa: 0.0 ± 0.0
Phe	4.928PheAla: 4.928 ± 0.058	0.326PheCys: 0.326 ± 0.014	2.731PheAsp: 2.731 ± 0.053	2.138PheGlu: 2.138 ± 0.038	1.299PhePhe: 1.299 ± 0.032	3.898PheGly: 3.898 ± 0.065	0.736PheHis: 0.736 ± 0.021	1.539PheIle: 1.539 ± 0.034	1.004PheLys: 1.004 ± 0.027	3.079PheLeu: 3.079 ± 0.047	0.729PheMet: 0.729 ± 0.024	1.113PheAsn: 1.113 ± 0.032	1.508PhePro: 1.508 ± 0.035	0.95PheGln: 0.95 ± 0.026	2.267PheArg: 2.267 ± 0.04	2.12PheSer: 2.12 ± 0.039	2.095PheThr: 2.095 ± 0.053	2.667PheVal: 2.667 ± 0.036	0.525PheTrp: 0.525 ± 0.018	0.957PheTyr: 0.957 ± 0.03	0.0PheXaa: 0.0 ± 0.0
Gly	10.13GlyAla: 10.13 ± 0.102	0.786GlyCys: 0.786 ± 0.022	4.946GlyAsp: 4.946 ± 0.079	4.916GlyGlu: 4.916 ± 0.061	3.759GlyPhe: 3.759 ± 0.048	8.337GlyGly: 8.337 ± 0.135	1.748GlyHis: 1.748 ± 0.039	4.489GlyIle: 4.489 ± 0.063	3.547GlyLys: 3.547 ± 0.052	8.492GlyLeu: 8.492 ± 0.08	2.38GlyMet: 2.38 ± 0.046	2.592GlyAsn: 2.592 ± 0.072	3.673GlyPro: 3.673 ± 0.051	2.871GlyGln: 2.871 ± 0.048	6.002GlyArg: 6.002 ± 0.073	5.154GlySer: 5.154 ± 0.076	5.21GlyThr: 5.21 ± 0.106	6.545GlyVal: 6.545 ± 0.074	1.634GlyTrp: 1.634 ± 0.037	2.438GlyTyr: 2.438 ± 0.043	0.0GlyXaa: 0.0 ± 0.0
His	2.374HisAla: 2.374 ± 0.046	0.205HisCys: 0.205 ± 0.011	1.11HisAsp: 1.11 ± 0.031	0.935HisGlu: 0.935 ± 0.022	0.823HisPhe: 0.823 ± 0.024	1.915HisGly: 1.915 ± 0.043	0.536HisHis: 0.536 ± 0.022	0.967HisIle: 0.967 ± 0.024	0.488HisLys: 0.488 ± 0.018	1.853HisLeu: 1.853 ± 0.036	0.441HisMet: 0.441 ± 0.018	0.48HisAsn: 0.48 ± 0.017	1.211HisPro: 1.211 ± 0.03	0.557HisGln: 0.557 ± 0.022	1.443HisArg: 1.443 ± 0.035	0.935HisSer: 0.935 ± 0.025	0.752HisThr: 0.752 ± 0.02	1.409HisVal: 1.409 ± 0.036	0.376HisTrp: 0.376 ± 0.017	0.578HisTyr: 0.578 ± 0.021	0.0HisXaa: 0.0 ± 0.0
Ile	7.968IleAla: 7.968 ± 0.077	0.445IleCys: 0.445 ± 0.017	3.986IleAsp: 3.986 ± 0.054	3.775IleGlu: 3.775 ± 0.061	1.634IlePhe: 1.634 ± 0.036	5.278IleGly: 5.278 ± 0.065	0.893IleHis: 0.893 ± 0.025	2.001IleIle: 2.001 ± 0.042	1.284IleLys: 1.284 ± 0.031	4.182IleLeu: 4.182 ± 0.057	0.837IleMet: 0.837 ± 0.026	1.407IleAsn: 1.407 ± 0.038	2.334IlePro: 2.334 ± 0.039	1.274IleGln: 1.274 ± 0.031	3.15IleArg: 3.15 ± 0.054	2.759IleSer: 2.759 ± 0.046	2.705IleThr: 2.705 ± 0.06	4.194IleVal: 4.194 ± 0.061	0.636IleTrp: 0.636 ± 0.02	1.07IleTyr: 1.07 ± 0.028	0.0IleXaa: 0.0 ± 0.0
Lys	4.076LysAla: 4.076 ± 0.061	0.174LysCys: 0.174 ± 0.01	1.578LysAsp: 1.578 ± 0.035	1.269LysGlu: 1.269 ± 0.033	0.977LysPhe: 0.977 ± 0.027	2.661LysGly: 2.661 ± 0.048	0.595LysHis: 0.595 ± 0.019	1.666LysIle: 1.666 ± 0.037	1.124LysLys: 1.124 ± 0.037	3.454LysLeu: 3.454 ± 0.051	0.831LysMet: 0.831 ± 0.025	0.793LysAsn: 0.793 ± 0.023	2.198LysPro: 2.198 ± 0.043	0.993LysGln: 0.993 ± 0.026	2.468LysArg: 2.468 ± 0.044	1.68LysSer: 1.68 ± 0.039	1.801LysThr: 1.801 ± 0.037	2.18LysVal: 2.18 ± 0.042	0.444LysTrp: 0.444 ± 0.019	0.624LysTyr: 0.624 ± 0.021	0.0LysXaa: 0.0 ± 0.0
Leu	13.631LeuAla: 13.631 ± 0.134	0.77LeuCys: 0.77 ± 0.024	5.97LeuAsp: 5.97 ± 0.076	4.783LeuGlu: 4.783 ± 0.066	3.531LeuPhe: 3.531 ± 0.057	8.378LeuGly: 8.378 ± 0.084	1.76LeuHis: 1.76 ± 0.039	5.126LeuIle: 5.126 ± 0.063	3.145LeuLys: 3.145 ± 0.05	9.242LeuLeu: 9.242 ± 0.125	2.122LeuMet: 2.122 ± 0.041	2.521LeuAsn: 2.521 ± 0.04	5.302LeuPro: 5.302 ± 0.062	2.463LeuGln: 2.463 ± 0.045	6.576LeuArg: 6.576 ± 0.085	5.978LeuSer: 5.978 ± 0.061	5.531LeuThr: 5.531 ± 0.073	6.832LeuVal: 6.832 ± 0.073	1.19LeuTrp: 1.19 ± 0.033	2.011LeuTyr: 2.011 ± 0.04	0.0LeuXaa: 0.0 ± 0.0
Met	3.067MetAla: 3.067 ± 0.057	0.149MetCys: 0.149 ± 0.01	1.048MetAsp: 1.048 ± 0.028	1.012MetGlu: 1.012 ± 0.029	0.761MetPhe: 0.761 ± 0.024	1.917MetGly: 1.917 ± 0.041	0.443MetHis: 0.443 ± 0.016	1.439MetIle: 1.439 ± 0.03	0.924MetLys: 0.924 ± 0.025	2.744MetLeu: 2.744 ± 0.043	0.657MetMet: 0.657 ± 0.024	0.634MetAsn: 0.634 ± 0.022	1.484MetPro: 1.484 ± 0.034	0.748MetGln: 0.748 ± 0.024	1.9MetArg: 1.9 ± 0.037	1.398MetSer: 1.398 ± 0.029	1.701MetThr: 1.701 ± 0.033	1.649MetVal: 1.649 ± 0.037	0.219MetTrp: 0.219 ± 0.011	0.288MetTyr: 0.288 ± 0.014	0.0MetXaa: 0.0 ± 0.0
Asn	3.393AsnAla: 3.393 ± 0.058	0.257AsnCys: 0.257 ± 0.013	1.538AsnAsp: 1.538 ± 0.06	1.181AsnGlu: 1.181 ± 0.029	1.08AsnPhe: 1.08 ± 0.029	2.615AsnGly: 2.615 ± 0.062	0.503AsnHis: 0.503 ± 0.019	1.372AsnIle: 1.372 ± 0.033	0.736AsnLys: 0.736 ± 0.022	2.605AsnLeu: 2.605 ± 0.05	0.573AsnMet: 0.573 ± 0.021	0.803AsnAsn: 0.803 ± 0.032	1.856AsnPro: 1.856 ± 0.036	0.82AsnGln: 0.82 ± 0.029	1.793AsnArg: 1.793 ± 0.032	1.449AsnSer: 1.449 ± 0.04	1.289AsnThr: 1.289 ± 0.041	2.064AsnVal: 2.064 ± 0.052	0.488AsnTrp: 0.488 ± 0.018	0.802AsnTyr: 0.802 ± 0.028	0.0AsnXaa: 0.0 ± 0.0
Pro	6.74ProAla: 6.74 ± 0.085	0.314ProCys: 0.314 ± 0.015	3.43ProAsp: 3.43 ± 0.049	3.662ProGlu: 3.662 ± 0.057	1.908ProPhe: 1.908 ± 0.036	4.84ProGly: 4.84 ± 0.062	1.007ProHis: 1.007 ± 0.031	2.571ProIle: 2.571 ± 0.045	1.66ProLys: 1.66 ± 0.037	4.859ProLeu: 4.859 ± 0.063	1.24ProMet: 1.24 ± 0.029	1.412ProAsn: 1.412 ± 0.035	2.504ProPro: 2.504 ± 0.057	1.632ProGln: 1.632 ± 0.034	2.991ProArg: 2.991 ± 0.049	2.761ProSer: 2.761 ± 0.047	2.64ProThr: 2.64 ± 0.051	4.304ProVal: 4.304 ± 0.062	0.682ProTrp: 0.682 ± 0.02	1.114ProTyr: 1.114 ± 0.025	0.0ProXaa: 0.0 ± 0.0
Gln	3.875GlnAla: 3.875 ± 0.055	0.192GlnCys: 0.192 ± 0.012	1.415GlnAsp: 1.415 ± 0.032	1.266GlnGlu: 1.266 ± 0.034	1.122GlnPhe: 1.122 ± 0.027	2.429GlnGly: 2.429 ± 0.036	0.617GlnHis: 0.617 ± 0.02	1.811GlnIle: 1.811 ± 0.039	0.914GlnLys: 0.914 ± 0.028	3.015GlnLeu: 3.015 ± 0.041	0.809GlnMet: 0.809 ± 0.026	0.822GlnAsn: 0.822 ± 0.027	1.773GlnPro: 1.773 ± 0.036	1.147GlnGln: 1.147 ± 0.033	2.475GlnArg: 2.475 ± 0.047	1.815GlnSer: 1.815 ± 0.037	1.68GlnThr: 1.68 ± 0.042	2.166GlnVal: 2.166 ± 0.04	0.431GlnTrp: 0.431 ± 0.021	0.641GlnTyr: 0.641 ± 0.023	0.0GlnXaa: 0.0 ± 0.0
Arg	8.464ArgAla: 8.464 ± 0.111	0.493ArgCys: 0.493 ± 0.022	4.173ArgAsp: 4.173 ± 0.056	4.05ArgGlu: 4.05 ± 0.059	3.077ArgPhe: 3.077 ± 0.051	5.202ArgGly: 5.202 ± 0.068	1.582ArgHis: 1.582 ± 0.037	4.138ArgIle: 4.138 ± 0.059	2.172ArgLys: 2.172 ± 0.038	7.364ArgLeu: 7.364 ± 0.096	1.859ArgMet: 1.859 ± 0.036	1.91ArgAsn: 1.91 ± 0.038	3.417ArgPro: 3.417 ± 0.056	2.29ArgGln: 2.29 ± 0.043	5.183ArgArg: 5.183 ± 0.081	3.709ArgSer: 3.709 ± 0.056	3.587ArgThr: 3.587 ± 0.054	4.739ArgVal: 4.739 ± 0.062	1.188ArgTrp: 1.188 ± 0.033	1.861ArgTyr: 1.861 ± 0.04	0.0ArgXaa: 0.0 ± 0.0
Ser	6.719SerAla: 6.719 ± 0.071	0.379SerCys: 0.379 ± 0.017	3.283SerAsp: 3.283 ± 0.047	2.827SerGlu: 2.827 ± 0.05	2.288SerPhe: 2.288 ± 0.039	5.755SerGly: 5.755 ± 0.065	1.007SerHis: 1.007 ± 0.026	2.963SerIle: 2.963 ± 0.061	1.586SerLys: 1.586 ± 0.032	5.07SerLeu: 5.07 ± 0.061	1.27SerMet: 1.27 ± 0.031	1.576SerAsn: 1.576 ± 0.031	2.917SerPro: 2.917 ± 0.054	1.598SerGln: 1.598 ± 0.035	3.585SerArg: 3.585 ± 0.055	2.998SerSer: 2.998 ± 0.062	2.788SerThr: 2.788 ± 0.059	3.893SerVal: 3.893 ± 0.061	0.831SerTrp: 0.831 ± 0.02	1.411SerTyr: 1.411 ± 0.036	0.0SerXaa: 0.0 ± 0.0
Thr	6.699ThrAla: 6.699 ± 0.105	0.377ThrCys: 0.377 ± 0.016	2.94ThrAsp: 2.94 ± 0.05	2.498ThrGlu: 2.498 ± 0.041	1.877ThrPhe: 1.877 ± 0.039	5.689ThrGly: 5.689 ± 0.098	0.944ThrHis: 0.944 ± 0.022	3.094ThrIle: 3.094 ± 0.055	1.467ThrLys: 1.467 ± 0.033	5.811ThrLeu: 5.811 ± 0.069	1.222ThrMet: 1.222 ± 0.029	1.515ThrAsn: 1.515 ± 0.051	3.52ThrPro: 3.52 ± 0.06	1.556ThrGln: 1.556 ± 0.04	3.511ThrArg: 3.511 ± 0.056	3.04ThrSer: 3.04 ± 0.072	2.94ThrThr: 2.94 ± 0.065	4.213ThrVal: 4.213 ± 0.086	0.633ThrTrp: 0.633 ± 0.02	1.251ThrTyr: 1.251 ± 0.043	0.0ThrXaa: 0.0 ± 0.0
Val	9.503ValAla: 9.503 ± 0.102	0.501ValCys: 0.501 ± 0.018	4.201ValAsp: 4.201 ± 0.059	4.3ValGlu: 4.3 ± 0.058	2.311ValPhe: 2.311 ± 0.041	5.414ValGly: 5.414 ± 0.062	1.288ValHis: 1.288 ± 0.025	3.725ValIle: 3.725 ± 0.06	2.082ValLys: 2.082 ± 0.044	6.445ValLeu: 6.445 ± 0.071	1.676ValMet: 1.676 ± 0.038	2.075ValAsn: 2.075 ± 0.045	3.937ValPro: 3.937 ± 0.054	2.058ValGln: 2.058 ± 0.043	5.093ValArg: 5.093 ± 0.062	4.315ValSer: 4.315 ± 0.06	4.839ValThr: 4.839 ± 0.095	5.223ValVal: 5.223 ± 0.063	0.886ValTrp: 0.886 ± 0.027	1.434ValTyr: 1.434 ± 0.028	0.0ValXaa: 0.0 ± 0.0
Trp	1.46TrpAla: 1.46 ± 0.038	0.132TrpCys: 0.132 ± 0.01	0.732TrpAsp: 0.732 ± 0.023	0.631TrpGlu: 0.631 ± 0.021	0.61TrpPhe: 0.61 ± 0.021	1.023TrpGly: 1.023 ± 0.032	0.32TrpHis: 0.32 ± 0.015	0.742TrpIle: 0.742 ± 0.022	0.526TrpLys: 0.526 ± 0.02	1.766TrpLeu: 1.766 ± 0.036	0.356TrpMet: 0.356 ± 0.015	0.491TrpAsn: 0.491 ± 0.02	0.699TrpPro: 0.699 ± 0.021	0.59TrpGln: 0.59 ± 0.02	1.271TrpArg: 1.271 ± 0.034	0.915TrpSer: 0.915 ± 0.026	0.855TrpThr: 0.855 ± 0.025	0.894TrpVal: 0.894 ± 0.024	0.265TrpTrp: 0.265 ± 0.014	0.311TrpTyr: 0.311 ± 0.015	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.763TyrAla: 2.763 ± 0.04	0.215TyrCys: 0.215 ± 0.012	1.55TyrAsp: 1.55 ± 0.04	1.159TyrGlu: 1.159 ± 0.029	0.945TyrPhe: 0.945 ± 0.026	2.25TyrGly: 2.25 ± 0.043	0.47TyrHis: 0.47 ± 0.017	0.88TyrIle: 0.88 ± 0.027	0.609TyrLys: 0.609 ± 0.02	2.125TyrLeu: 2.125 ± 0.038	0.411TyrMet: 0.411 ± 0.017	0.676TyrAsn: 0.676 ± 0.028	1.089TyrPro: 1.089 ± 0.029	0.738TyrGln: 0.738 ± 0.022	1.89TyrArg: 1.89 ± 0.039	1.402TyrSer: 1.402 ± 0.029	1.138TyrThr: 1.138 ± 0.045	1.57TyrVal: 1.57 ± 0.033	0.377TyrTrp: 0.377 ± 0.016	0.637TyrTyr: 0.637 ± 0.022	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4688 proteins (1519867 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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