Dongia mobilis
Average proteome isoelectric point is 6.58
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3804 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4V3DF21|A0A4V3DF21_9PROT Tfp pilus assembly protein PilF OS=Dongia mobilis OX=578943 GN=A8950_0922 PE=4 SV=1
MM1 pKa = 7.12 VPVAIANLGNLEE13 pKa = 4.15 MKK15 pKa = 10.29 KK16 pKa = 10.52 VLLGTSALLGVGLLAGTAQASDD38 pKa = 3.89 GVKK41 pKa = 10.51 LSLGGYY47 pKa = 6.56 MTNQMGVAFDD57 pKa = 4.69 DD58 pKa = 4.76 DD59 pKa = 4.39 SAGEE63 pKa = 4.12 AGDD66 pKa = 4.17 NINAFGVGTDD76 pKa = 3.38 AEE78 pKa = 4.69 IYY80 pKa = 10.58 FIGSVTLDD88 pKa = 3.1 NGVTVGVRR96 pKa = 11.84 FDD98 pKa = 4.9 LEE100 pKa = 4.98 GGDD103 pKa = 4.07 EE104 pKa = 4.87 NNDD107 pKa = 3.82 QMDD110 pKa = 3.16 QAYY113 pKa = 9.9 AYY115 pKa = 9.73 FKK117 pKa = 11.17 GGFGDD122 pKa = 3.85 IRR124 pKa = 11.84 IGSQAGAAGNMYY136 pKa = 8.76 MLPPGSTANFGPYY149 pKa = 9.84 SPNTTGSALSPGVFDD164 pKa = 5.32 PEE166 pKa = 4.42 GMLANQDD173 pKa = 3.38 KK174 pKa = 10.21 SQKK177 pKa = 9.88 LVYY180 pKa = 10.32 YY181 pKa = 9.65 SPNWSGFSFGVSFTPNDD198 pKa = 3.33 NEE200 pKa = 4.0 KK201 pKa = 10.99 DD202 pKa = 3.88 YY203 pKa = 11.79 NNGDD207 pKa = 3.63 NADD210 pKa = 4.02 GQWRR214 pKa = 11.84 AASAQGDD221 pKa = 3.76 SDD223 pKa = 4.37 NNIGLGLHH231 pKa = 5.58 YY232 pKa = 9.55 THH234 pKa = 7.17 EE235 pKa = 4.85 GDD237 pKa = 4.55 GWGLDD242 pKa = 3.93 LGAAAYY248 pKa = 9.24 WEE250 pKa = 4.62 GDD252 pKa = 3.6 VQQKK256 pKa = 9.63 VAGQDD261 pKa = 3.18 EE262 pKa = 4.41 QAGYY266 pKa = 9.87 NAGINLSFGGFAFGVAGTYY285 pKa = 10.61 LDD287 pKa = 4.89 DD288 pKa = 5.1 GNGAGQDD295 pKa = 2.75 IWSIGTGMTYY305 pKa = 10.38 NVEE308 pKa = 3.64 AWTVGLGWAHH318 pKa = 7.25 AEE320 pKa = 4.18 LEE322 pKa = 4.48 VAGARR327 pKa = 11.84 DD328 pKa = 3.46 ASADD332 pKa = 3.7 RR333 pKa = 11.84 VGVTGNYY340 pKa = 10.83 AMGPGIDD347 pKa = 3.78 LDD349 pKa = 4.12 AGIFYY354 pKa = 9.0 TWGEE358 pKa = 4.15 DD359 pKa = 3.39 AVGEE363 pKa = 4.04 GDD365 pKa = 3.48 YY366 pKa = 11.4 DD367 pKa = 4.39 SIEE370 pKa = 4.34 FGVGSSISFF379 pKa = 3.54
Molecular weight: 39.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.714
IPC2_protein 3.656
IPC_protein 3.694
Toseland 3.465
ProMoST 3.872
Dawson 3.694
Bjellqvist 3.846
Wikipedia 3.643
Rodwell 3.516
Grimsley 3.363
Solomon 3.681
Lehninger 3.643
Nozaki 3.808
DTASelect 4.075
Thurlkill 3.516
EMBOSS 3.656
Sillero 3.821
Patrickios 0.757
IPC_peptide 3.681
IPC2_peptide 3.795
IPC2.peptide.svr19 3.726
Protein with the highest isoelectric point:
>tr|A0A4R6WN28|A0A4R6WN28_9PROT DNA helicase OS=Dongia mobilis OX=578943 GN=A8950_2245 PE=3 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 10.27 QPSKK9 pKa = 9.73 LVRR12 pKa = 11.84 KK13 pKa = 8.95 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.63 GFRR19 pKa = 11.84 SRR21 pKa = 11.84 MATNGGQKK29 pKa = 10.15 VISSRR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.8 GRR39 pKa = 11.84 KK40 pKa = 8.92 RR41 pKa = 11.84 LSAA44 pKa = 3.96
Molecular weight: 5.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.413
IPC2_protein 11.096
IPC_protein 12.457
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.34
Grimsley 12.661
Solomon 13.1
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.076
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.034
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3804
0
3804
1260373
27
4980
331.3
35.87
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.2 ± 0.064
0.886 ± 0.012
5.683 ± 0.042
5.538 ± 0.042
3.64 ± 0.024
8.824 ± 0.044
2.061 ± 0.019
5.361 ± 0.029
3.16 ± 0.032
10.651 ± 0.049
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.478 ± 0.019
2.574 ± 0.027
5.219 ± 0.031
3.313 ± 0.026
7.144 ± 0.049
4.845 ± 0.026
4.807 ± 0.033
7.072 ± 0.032
1.343 ± 0.017
2.201 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here