Amino acid dipepetide frequency for Pseudomonas phage Fc02

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
17.763AlaAla: 17.763 ± 2.617
1.054AlaCys: 1.054 ± 0.391
6.732AlaAsp: 6.732 ± 0.526
8.598AlaGlu: 8.598 ± 0.775
3.082AlaPhe: 3.082 ± 0.532
10.788AlaGly: 10.788 ± 0.97
1.622AlaHis: 1.622 ± 0.416
5.759AlaIle: 5.759 ± 0.596
4.542AlaLys: 4.542 ± 0.756
12.734AlaLeu: 12.734 ± 1.007
3.731AlaMet: 3.731 ± 0.571
2.758AlaAsn: 2.758 ± 0.482
5.029AlaPro: 5.029 ± 0.569
6.408AlaGln: 6.408 ± 0.771
8.192AlaArg: 8.192 ± 0.745
6.975AlaSer: 6.975 ± 0.834
6.002AlaThr: 6.002 ± 0.836
6.408AlaVal: 6.408 ± 0.693
2.92AlaTrp: 2.92 ± 0.423
2.433AlaTyr: 2.433 ± 0.431
0.0AlaXaa: 0.0 ± 0.0
Cys
0.73CysAla: 0.73 ± 0.273
0.243CysCys: 0.243 ± 0.132
0.649CysAsp: 0.649 ± 0.173
0.324CysGlu: 0.324 ± 0.148
0.162CysPhe: 0.162 ± 0.12
0.73CysGly: 0.73 ± 0.225
0.243CysHis: 0.243 ± 0.127
0.406CysIle: 0.406 ± 0.185
0.324CysLys: 0.324 ± 0.164
0.406CysLeu: 0.406 ± 0.174
0.162CysMet: 0.162 ± 0.106
0.406CysAsn: 0.406 ± 0.175
0.406CysPro: 0.406 ± 0.17
0.487CysGln: 0.487 ± 0.192
0.811CysArg: 0.811 ± 0.274
0.73CysSer: 0.73 ± 0.294
0.406CysThr: 0.406 ± 0.206
0.406CysVal: 0.406 ± 0.211
0.0CysTrp: 0.0 ± 0.0
0.324CysTyr: 0.324 ± 0.139
0.0CysXaa: 0.0 ± 0.0
Asp
6.57AspAla: 6.57 ± 0.762
0.162AspCys: 0.162 ± 0.113
3.407AspAsp: 3.407 ± 0.517
4.542AspGlu: 4.542 ± 0.621
2.352AspPhe: 2.352 ± 0.411
5.597AspGly: 5.597 ± 0.522
0.892AspHis: 0.892 ± 0.286
3.082AspIle: 3.082 ± 0.498
1.541AspLys: 1.541 ± 0.321
5.353AspLeu: 5.353 ± 0.478
0.973AspMet: 0.973 ± 0.248
1.541AspAsn: 1.541 ± 0.289
2.677AspPro: 2.677 ± 0.428
3.569AspGln: 3.569 ± 0.547
3.407AspArg: 3.407 ± 0.587
2.677AspSer: 2.677 ± 0.445
2.596AspThr: 2.596 ± 0.394
4.623AspVal: 4.623 ± 0.542
0.892AspTrp: 0.892 ± 0.246
1.622AspTyr: 1.622 ± 0.305
0.0AspXaa: 0.0 ± 0.0
Glu
8.435GluAla: 8.435 ± 1.097
0.892GluCys: 0.892 ± 0.247
3.325GluAsp: 3.325 ± 0.587
4.299GluGlu: 4.299 ± 0.738
1.784GluPhe: 1.784 ± 0.456
2.352GluGly: 2.352 ± 0.443
1.622GluHis: 1.622 ± 0.444
2.92GluIle: 2.92 ± 0.541
3.488GluLys: 3.488 ± 0.603
7.543GluLeu: 7.543 ± 0.807
1.217GluMet: 1.217 ± 0.333
2.028GluAsn: 2.028 ± 0.438
1.947GluPro: 1.947 ± 0.532
5.191GluGln: 5.191 ± 0.719
5.597GluArg: 5.597 ± 0.682
3.488GluSer: 3.488 ± 0.515
2.19GluThr: 2.19 ± 0.348
3.244GluVal: 3.244 ± 0.523
1.217GluTrp: 1.217 ± 0.31
1.866GluTyr: 1.866 ± 0.308
0.0GluXaa: 0.0 ± 0.0
Phe
2.677PheAla: 2.677 ± 0.49
0.406PheCys: 0.406 ± 0.192
2.677PheAsp: 2.677 ± 0.477
1.947PheGlu: 1.947 ± 0.403
0.649PhePhe: 0.649 ± 0.193
2.92PheGly: 2.92 ± 0.447
0.406PheHis: 0.406 ± 0.134
1.054PheIle: 1.054 ± 0.282
0.568PheLys: 0.568 ± 0.253
2.514PheLeu: 2.514 ± 0.408
0.568PheMet: 0.568 ± 0.184
1.541PheAsn: 1.541 ± 0.344
1.054PhePro: 1.054 ± 0.299
1.379PheGln: 1.379 ± 0.34
2.92PheArg: 2.92 ± 0.504
1.298PheSer: 1.298 ± 0.346
1.703PheThr: 1.703 ± 0.385
1.703PheVal: 1.703 ± 0.358
0.324PheTrp: 0.324 ± 0.179
0.568PheTyr: 0.568 ± 0.178
0.0PheXaa: 0.0 ± 0.0
Gly
8.111GlyAla: 8.111 ± 1.322
0.892GlyCys: 0.892 ± 0.278
4.299GlyAsp: 4.299 ± 0.544
4.218GlyGlu: 4.218 ± 0.498
2.433GlyPhe: 2.433 ± 0.351
5.272GlyGly: 5.272 ± 0.644
1.298GlyHis: 1.298 ± 0.337
3.325GlyIle: 3.325 ± 0.412
4.299GlyLys: 4.299 ± 0.631
7.949GlyLeu: 7.949 ± 0.898
1.784GlyMet: 1.784 ± 0.288
3.163GlyAsn: 3.163 ± 0.516
2.433GlyPro: 2.433 ± 0.51
4.299GlyGln: 4.299 ± 0.628
4.704GlyArg: 4.704 ± 0.598
3.65GlySer: 3.65 ± 0.637
4.461GlyThr: 4.461 ± 0.726
5.678GlyVal: 5.678 ± 0.641
2.028GlyTrp: 2.028 ± 0.313
2.028GlyTyr: 2.028 ± 0.519
0.0GlyXaa: 0.0 ± 0.0
His
1.866HisAla: 1.866 ± 0.375
0.081HisCys: 0.081 ± 0.08
0.973HisAsp: 0.973 ± 0.244
1.298HisGlu: 1.298 ± 0.275
0.324HisPhe: 0.324 ± 0.137
1.622HisGly: 1.622 ± 0.408
0.406HisHis: 0.406 ± 0.167
0.811HisIle: 0.811 ± 0.236
0.649HisLys: 0.649 ± 0.196
1.866HisLeu: 1.866 ± 0.507
0.162HisMet: 0.162 ± 0.104
0.811HisAsn: 0.811 ± 0.249
0.892HisPro: 0.892 ± 0.32
1.541HisGln: 1.541 ± 0.413
1.217HisArg: 1.217 ± 0.294
1.217HisSer: 1.217 ± 0.395
0.73HisThr: 0.73 ± 0.219
0.892HisVal: 0.892 ± 0.277
0.162HisTrp: 0.162 ± 0.103
0.162HisTyr: 0.162 ± 0.11
0.0HisXaa: 0.0 ± 0.0
Ile
5.759IleAla: 5.759 ± 0.669
0.324IleCys: 0.324 ± 0.179
3.082IleAsp: 3.082 ± 0.439
4.137IleGlu: 4.137 ± 0.612
0.73IlePhe: 0.73 ± 0.198
2.352IleGly: 2.352 ± 0.391
1.054IleHis: 1.054 ± 0.297
1.054IleIle: 1.054 ± 0.354
1.217IleLys: 1.217 ± 0.255
3.325IleLeu: 3.325 ± 0.436
0.406IleMet: 0.406 ± 0.202
1.46IleAsn: 1.46 ± 0.298
1.947IlePro: 1.947 ± 0.387
1.703IleGln: 1.703 ± 0.356
3.488IleArg: 3.488 ± 0.489
3.001IleSer: 3.001 ± 0.596
3.163IleThr: 3.163 ± 0.514
1.784IleVal: 1.784 ± 0.369
0.811IleTrp: 0.811 ± 0.391
1.054IleTyr: 1.054 ± 0.274
0.0IleXaa: 0.0 ± 0.0
Lys
6.164LysAla: 6.164 ± 0.715
0.243LysCys: 0.243 ± 0.126
2.19LysAsp: 2.19 ± 0.434
2.514LysGlu: 2.514 ± 0.511
0.811LysPhe: 0.811 ± 0.226
2.271LysGly: 2.271 ± 0.475
0.73LysHis: 0.73 ± 0.205
1.298LysIle: 1.298 ± 0.343
1.866LysLys: 1.866 ± 0.324
4.055LysLeu: 4.055 ± 0.619
0.406LysMet: 0.406 ± 0.187
0.973LysAsn: 0.973 ± 0.259
2.109LysPro: 2.109 ± 0.603
1.298LysGln: 1.298 ± 0.361
3.893LysArg: 3.893 ± 0.698
2.109LysSer: 2.109 ± 0.458
1.947LysThr: 1.947 ± 0.402
1.947LysVal: 1.947 ± 0.386
0.324LysTrp: 0.324 ± 0.163
0.811LysTyr: 0.811 ± 0.22
0.0LysXaa: 0.0 ± 0.0
Leu
12.41LeuAla: 12.41 ± 0.814
1.054LeuCys: 1.054 ± 0.323
7.381LeuAsp: 7.381 ± 0.601
5.515LeuGlu: 5.515 ± 0.612
2.352LeuPhe: 2.352 ± 0.487
7.624LeuGly: 7.624 ± 0.778
2.028LeuHis: 2.028 ± 0.422
4.137LeuIle: 4.137 ± 0.63
3.65LeuLys: 3.65 ± 0.603
9.165LeuLeu: 9.165 ± 0.875
2.028LeuMet: 2.028 ± 0.389
3.001LeuAsn: 3.001 ± 0.475
5.515LeuPro: 5.515 ± 0.668
5.678LeuGln: 5.678 ± 0.719
7.949LeuArg: 7.949 ± 0.776
5.921LeuSer: 5.921 ± 0.676
6.327LeuThr: 6.327 ± 0.878
7.624LeuVal: 7.624 ± 0.694
0.649LeuTrp: 0.649 ± 0.255
2.109LeuTyr: 2.109 ± 0.45
0.0LeuXaa: 0.0 ± 0.0
Met
2.596MetAla: 2.596 ± 0.429
0.0MetCys: 0.0 ± 0.0
1.054MetAsp: 1.054 ± 0.29
1.703MetGlu: 1.703 ± 0.369
0.649MetPhe: 0.649 ± 0.185
2.271MetGly: 2.271 ± 0.419
0.324MetHis: 0.324 ± 0.194
1.379MetIle: 1.379 ± 0.374
1.054MetLys: 1.054 ± 0.268
1.622MetLeu: 1.622 ± 0.371
0.243MetMet: 0.243 ± 0.124
0.649MetAsn: 0.649 ± 0.312
0.811MetPro: 0.811 ± 0.213
0.973MetGln: 0.973 ± 0.292
1.379MetArg: 1.379 ± 0.285
1.947MetSer: 1.947 ± 0.363
1.46MetThr: 1.46 ± 0.354
1.054MetVal: 1.054 ± 0.274
0.162MetTrp: 0.162 ± 0.116
0.0MetTyr: 0.0 ± 0.0
0.0MetXaa: 0.0 ± 0.0
Asn
3.893AsnAla: 3.893 ± 0.542
0.162AsnCys: 0.162 ± 0.141
1.054AsnAsp: 1.054 ± 0.243
1.947AsnGlu: 1.947 ± 0.293
0.892AsnPhe: 0.892 ± 0.223
3.001AsnGly: 3.001 ± 0.459
0.73AsnHis: 0.73 ± 0.231
0.406AsnIle: 0.406 ± 0.165
0.973AsnLys: 0.973 ± 0.271
3.163AsnLeu: 3.163 ± 0.498
0.487AsnMet: 0.487 ± 0.168
0.973AsnAsn: 0.973 ± 0.385
1.622AsnPro: 1.622 ± 0.399
1.784AsnGln: 1.784 ± 0.385
2.758AsnArg: 2.758 ± 0.41
1.217AsnSer: 1.217 ± 0.284
1.866AsnThr: 1.866 ± 0.448
1.784AsnVal: 1.784 ± 0.304
0.73AsnTrp: 0.73 ± 0.222
1.054AsnTyr: 1.054 ± 0.294
0.0AsnXaa: 0.0 ± 0.0
Pro
6.327ProAla: 6.327 ± 0.769
0.243ProCys: 0.243 ± 0.149
2.758ProAsp: 2.758 ± 0.552
2.758ProGlu: 2.758 ± 0.412
1.784ProPhe: 1.784 ± 0.394
4.38ProGly: 4.38 ± 0.778
0.324ProHis: 0.324 ± 0.163
1.298ProIle: 1.298 ± 0.287
1.46ProLys: 1.46 ± 0.386
3.974ProLeu: 3.974 ± 0.597
1.46ProMet: 1.46 ± 0.346
1.054ProAsn: 1.054 ± 0.243
2.271ProPro: 2.271 ± 0.517
1.703ProGln: 1.703 ± 0.29
4.137ProArg: 4.137 ± 0.552
3.163ProSer: 3.163 ± 0.441
2.596ProThr: 2.596 ± 0.403
3.893ProVal: 3.893 ± 0.696
0.892ProTrp: 0.892 ± 0.278
1.703ProTyr: 1.703 ± 0.435
0.0ProXaa: 0.0 ± 0.0
Gln
6.732GlnAla: 6.732 ± 0.818
0.324GlnCys: 0.324 ± 0.15
2.677GlnAsp: 2.677 ± 0.425
2.758GlnGlu: 2.758 ± 0.534
2.028GlnPhe: 2.028 ± 0.369
2.271GlnGly: 2.271 ± 0.4
0.811GlnHis: 0.811 ± 0.247
1.947GlnIle: 1.947 ± 0.301
1.784GlnLys: 1.784 ± 0.589
7.381GlnLeu: 7.381 ± 0.695
0.973GlnMet: 0.973 ± 0.27
0.973GlnAsn: 0.973 ± 0.346
3.569GlnPro: 3.569 ± 0.575
3.488GlnGln: 3.488 ± 0.596
3.569GlnArg: 3.569 ± 0.456
2.109GlnSer: 2.109 ± 0.364
2.677GlnThr: 2.677 ± 0.38
3.812GlnVal: 3.812 ± 0.393
0.73GlnTrp: 0.73 ± 0.244
1.217GlnTyr: 1.217 ± 0.374
0.0GlnXaa: 0.0 ± 0.0
Arg
7.381ArgAla: 7.381 ± 0.667
0.568ArgCys: 0.568 ± 0.223
4.623ArgAsp: 4.623 ± 0.432
4.867ArgGlu: 4.867 ± 0.719
1.784ArgPhe: 1.784 ± 0.336
5.353ArgGly: 5.353 ± 0.638
1.866ArgHis: 1.866 ± 0.406
3.812ArgIle: 3.812 ± 0.645
3.163ArgLys: 3.163 ± 0.714
9.003ArgLeu: 9.003 ± 0.704
1.703ArgMet: 1.703 ± 0.363
1.947ArgAsn: 1.947 ± 0.451
2.839ArgPro: 2.839 ± 0.526
3.974ArgGln: 3.974 ± 0.71
5.921ArgArg: 5.921 ± 0.764
4.137ArgSer: 4.137 ± 0.523
2.433ArgThr: 2.433 ± 0.442
4.623ArgVal: 4.623 ± 0.797
1.784ArgTrp: 1.784 ± 0.412
2.109ArgTyr: 2.109 ± 0.419
0.0ArgXaa: 0.0 ± 0.0
Ser
8.354SerAla: 8.354 ± 0.838
0.406SerCys: 0.406 ± 0.165
3.244SerAsp: 3.244 ± 0.545
3.325SerGlu: 3.325 ± 0.486
2.271SerPhe: 2.271 ± 0.458
4.299SerGly: 4.299 ± 0.556
0.406SerHis: 0.406 ± 0.181
2.433SerIle: 2.433 ± 0.416
2.028SerLys: 2.028 ± 0.379
6.489SerLeu: 6.489 ± 0.552
1.136SerMet: 1.136 ± 0.315
2.028SerAsn: 2.028 ± 0.395
3.407SerPro: 3.407 ± 0.536
1.784SerGln: 1.784 ± 0.402
3.731SerArg: 3.731 ± 0.605
3.569SerSer: 3.569 ± 0.507
3.001SerThr: 3.001 ± 0.332
3.082SerVal: 3.082 ± 0.537
0.811SerTrp: 0.811 ± 0.349
1.46SerTyr: 1.46 ± 0.301
0.0SerXaa: 0.0 ± 0.0
Thr
6.813ThrAla: 6.813 ± 0.859
0.324ThrCys: 0.324 ± 0.165
2.028ThrAsp: 2.028 ± 0.416
2.92ThrGlu: 2.92 ± 0.513
1.541ThrPhe: 1.541 ± 0.31
6.164ThrGly: 6.164 ± 0.915
1.054ThrHis: 1.054 ± 0.259
1.866ThrIle: 1.866 ± 0.391
1.622ThrLys: 1.622 ± 0.333
4.948ThrLeu: 4.948 ± 0.605
0.973ThrMet: 0.973 ± 0.234
2.028ThrAsn: 2.028 ± 0.364
3.731ThrPro: 3.731 ± 0.434
1.703ThrGln: 1.703 ± 0.359
3.001ThrArg: 3.001 ± 0.472
3.731ThrSer: 3.731 ± 0.466
2.514ThrThr: 2.514 ± 0.448
3.65ThrVal: 3.65 ± 0.543
0.811ThrTrp: 0.811 ± 0.305
1.46ThrTyr: 1.46 ± 0.369
0.0ThrXaa: 0.0 ± 0.0
Val
6.894ValAla: 6.894 ± 0.858
0.568ValCys: 0.568 ± 0.181
4.218ValAsp: 4.218 ± 0.505
4.704ValGlu: 4.704 ± 0.709
1.784ValPhe: 1.784 ± 0.307
3.974ValGly: 3.974 ± 0.488
1.136ValHis: 1.136 ± 0.303
3.001ValIle: 3.001 ± 0.506
2.433ValLys: 2.433 ± 0.483
6.164ValLeu: 6.164 ± 0.643
1.379ValMet: 1.379 ± 0.324
1.947ValAsn: 1.947 ± 0.359
3.569ValPro: 3.569 ± 0.412
2.839ValGln: 2.839 ± 0.466
3.731ValArg: 3.731 ± 0.546
3.569ValSer: 3.569 ± 0.563
4.38ValThr: 4.38 ± 0.575
4.867ValVal: 4.867 ± 0.77
1.379ValTrp: 1.379 ± 0.379
1.054ValTyr: 1.054 ± 0.33
0.0ValXaa: 0.0 ± 0.0
Trp
1.379TrpAla: 1.379 ± 0.337
0.243TrpCys: 0.243 ± 0.136
0.649TrpAsp: 0.649 ± 0.231
0.324TrpGlu: 0.324 ± 0.16
0.973TrpPhe: 0.973 ± 0.232
0.973TrpGly: 0.973 ± 0.287
0.324TrpHis: 0.324 ± 0.156
1.136TrpIle: 1.136 ± 0.312
0.406TrpLys: 0.406 ± 0.175
2.109TrpLeu: 2.109 ± 0.426
0.73TrpMet: 0.73 ± 0.263
0.487TrpAsn: 0.487 ± 0.178
0.892TrpPro: 0.892 ± 0.386
1.217TrpGln: 1.217 ± 0.311
1.46TrpArg: 1.46 ± 0.234
1.054TrpSer: 1.054 ± 0.266
1.054TrpThr: 1.054 ± 0.27
1.217TrpVal: 1.217 ± 0.319
0.649TrpTrp: 0.649 ± 0.294
0.324TrpTyr: 0.324 ± 0.156
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.677TyrAla: 2.677 ± 0.444
0.162TyrCys: 0.162 ± 0.12
1.054TyrAsp: 1.054 ± 0.254
2.028TyrGlu: 2.028 ± 0.324
0.487TyrPhe: 0.487 ± 0.172
2.109TyrGly: 2.109 ± 0.394
0.406TyrHis: 0.406 ± 0.293
0.73TyrIle: 0.73 ± 0.235
0.892TyrLys: 0.892 ± 0.269
2.352TyrLeu: 2.352 ± 0.339
0.73TyrMet: 0.73 ± 0.251
0.73TyrAsn: 0.73 ± 0.201
1.46TyrPro: 1.46 ± 0.533
0.811TyrGln: 0.811 ± 0.257
2.028TyrArg: 2.028 ± 0.312
1.703TyrSer: 1.703 ± 0.374
1.379TyrThr: 1.379 ± 0.381
1.298TyrVal: 1.298 ± 0.291
0.324TyrTrp: 0.324 ± 0.148
0.162TyrTyr: 0.162 ± 0.106
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 54 proteins (12330 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski