Delftia acidovorans (strain DSM 14801 / SPH-1)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Comamonadaceae; Delftia; Delftia acidovorans

Average proteome isoelectric point is 6.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5968 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A9C0X4|A9C0X4_DELAS ABC transporter related OS=Delftia acidovorans (strain DSM 14801 / SPH-1) OX=398578 GN=Daci_5809 PE=4 SV=1
MM1 pKa = 6.22TTAPIPVSADD11 pKa = 3.01SAASADD17 pKa = 4.3PMPQPPEE24 pKa = 4.0QPDD27 pKa = 3.63LDD29 pKa = 4.02ACCGNGCDD37 pKa = 3.59PCIFDD42 pKa = 5.22SYY44 pKa = 11.25DD45 pKa = 3.17LAMDD49 pKa = 4.64EE50 pKa = 4.14YY51 pKa = 10.78RR52 pKa = 11.84QALRR56 pKa = 11.84AWKK59 pKa = 10.03ARR61 pKa = 11.84NEE63 pKa = 4.17TPAGQGG69 pKa = 3.1

Molecular weight: 7.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A9C1X9|A9C1X9_DELAS Outer membrane lipoprotein Blc OS=Delftia acidovorans (strain DSM 14801 / SPH-1) OX=398578 GN=Daci_2977 PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.25QPSKK9 pKa = 7.79TRR11 pKa = 11.84RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.79GFLVRR21 pKa = 11.84MKK23 pKa = 9.4TKK25 pKa = 10.4GGRR28 pKa = 11.84AVINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.58GRR39 pKa = 11.84KK40 pKa = 8.75RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5968

0

5968

2005225

35

6176

336.0

36.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.273 ± 0.044

0.963 ± 0.011

5.077 ± 0.025

5.089 ± 0.027

3.221 ± 0.019

8.507 ± 0.038

2.319 ± 0.018

4.086 ± 0.024

2.877 ± 0.033

10.823 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.536 ± 0.015

2.452 ± 0.022

5.426 ± 0.025

4.726 ± 0.028

7.01 ± 0.031

5.608 ± 0.03

5.021 ± 0.031

7.308 ± 0.028

1.539 ± 0.014

2.139 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski