Amino acid dipepetide frequency for Flavobacteria bacterium BAL38

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	4.058AlaAla: 4.058 ± 0.109	0.532AlaCys: 0.532 ± 0.03	3.07AlaAsp: 3.07 ± 0.074	3.767AlaGlu: 3.767 ± 0.093	3.393AlaPhe: 3.393 ± 0.07	4.176AlaGly: 4.176 ± 0.113	0.945AlaHis: 0.945 ± 0.034	5.503AlaIle: 5.503 ± 0.102	4.881AlaLys: 4.881 ± 0.09	5.691AlaLeu: 5.691 ± 0.089	1.447AlaMet: 1.447 ± 0.049	3.706AlaAsn: 3.706 ± 0.096	1.839AlaPro: 1.839 ± 0.054	2.43AlaGln: 2.43 ± 0.058	1.734AlaArg: 1.734 ± 0.055	4.144AlaSer: 4.144 ± 0.121	4.275AlaThr: 4.275 ± 0.133	3.921AlaVal: 3.921 ± 0.082	0.589AlaTrp: 0.589 ± 0.038	2.262AlaTyr: 2.262 ± 0.053	0.0AlaXaa: 0.0 ± 0.0
Cys	0.496CysAla: 0.496 ± 0.03	0.096CysCys: 0.096 ± 0.013	0.431CysAsp: 0.431 ± 0.024	0.498CysGlu: 0.498 ± 0.029	0.421CysPhe: 0.421 ± 0.026	0.61CysGly: 0.61 ± 0.032	0.167CysHis: 0.167 ± 0.016	0.592CysIle: 0.592 ± 0.025	0.531CysLys: 0.531 ± 0.026	0.62CysLeu: 0.62 ± 0.03	0.148CysMet: 0.148 ± 0.015	0.507CysAsn: 0.507 ± 0.026	0.313CysPro: 0.313 ± 0.024	0.233CysGln: 0.233 ± 0.017	0.19CysArg: 0.19 ± 0.015	0.665CysSer: 0.665 ± 0.05	0.494CysThr: 0.494 ± 0.043	0.463CysVal: 0.463 ± 0.027	0.058CysTrp: 0.058 ± 0.009	0.325CysTyr: 0.325 ± 0.019	0.0CysXaa: 0.0 ± 0.0
Asp	3.514AspAla: 3.514 ± 0.075	0.422AspCys: 0.422 ± 0.024	2.603AspAsp: 2.603 ± 0.061	3.658AspGlu: 3.658 ± 0.081	3.747AspPhe: 3.747 ± 0.069	3.444AspGly: 3.444 ± 0.121	0.635AspHis: 0.635 ± 0.027	4.124AspIle: 4.124 ± 0.071	4.283AspLys: 4.283 ± 0.081	4.931AspLeu: 4.931 ± 0.081	1.051AspMet: 1.051 ± 0.041	3.159AspAsn: 3.159 ± 0.077	1.304AspPro: 1.304 ± 0.05	1.201AspGln: 1.201 ± 0.04	1.525AspArg: 1.525 ± 0.047	2.932AspSer: 2.932 ± 0.064	2.514AspThr: 2.514 ± 0.057	3.538AspVal: 3.538 ± 0.081	0.716AspTrp: 0.716 ± 0.034	2.694AspTyr: 2.694 ± 0.063	0.0AspXaa: 0.0 ± 0.0
Glu	4.089GluAla: 4.089 ± 0.085	0.365GluCys: 0.365 ± 0.02	3.119GluAsp: 3.119 ± 0.074	4.674GluGlu: 4.674 ± 0.101	3.153GluPhe: 3.153 ± 0.071	3.459GluGly: 3.459 ± 0.068	1.032GluHis: 1.032 ± 0.036	6.197GluIle: 6.197 ± 0.106	6.379GluLys: 6.379 ± 0.109	6.079GluLeu: 6.079 ± 0.105	1.732GluMet: 1.732 ± 0.046	5.372GluAsn: 5.372 ± 0.09	1.462GluPro: 1.462 ± 0.047	2.205GluGln: 2.205 ± 0.058	2.228GluArg: 2.228 ± 0.063	3.232GluSer: 3.232 ± 0.07	3.687GluThr: 3.687 ± 0.07	4.323GluVal: 4.323 ± 0.091	0.578GluTrp: 0.578 ± 0.026	2.26GluTyr: 2.26 ± 0.06	0.0GluXaa: 0.0 ± 0.0
Phe	3.115PheAla: 3.115 ± 0.074	0.508PheCys: 0.508 ± 0.029	3.335PheAsp: 3.335 ± 0.062	3.766PheGlu: 3.766 ± 0.07	3.11PhePhe: 3.11 ± 0.073	3.802PheGly: 3.802 ± 0.061	0.879PheHis: 0.879 ± 0.035	4.269PheIle: 4.269 ± 0.093	3.956PheLys: 3.956 ± 0.083	4.978PheLeu: 4.978 ± 0.107	1.169PheMet: 1.169 ± 0.039	3.625PheAsn: 3.625 ± 0.072	1.815PhePro: 1.815 ± 0.05	1.808PheGln: 1.808 ± 0.048	1.577PheArg: 1.577 ± 0.047	4.468PheSer: 4.468 ± 0.08	3.353PheThr: 3.353 ± 0.076	3.337PheVal: 3.337 ± 0.069	0.595PheTrp: 0.595 ± 0.027	2.422PheTyr: 2.422 ± 0.06	0.0PheXaa: 0.0 ± 0.0
Gly	4.103GlyAla: 4.103 ± 0.093	0.647GlyCys: 0.647 ± 0.04	3.082GlyAsp: 3.082 ± 0.076	3.27GlyGlu: 3.27 ± 0.064	3.811GlyPhe: 3.811 ± 0.079	4.382GlyGly: 4.382 ± 0.111	1.047GlyHis: 1.047 ± 0.039	5.451GlyIle: 5.451 ± 0.093	5.066GlyLys: 5.066 ± 0.096	5.475GlyLeu: 5.475 ± 0.093	1.595GlyMet: 1.595 ± 0.041	3.795GlyAsn: 3.795 ± 0.079	1.312GlyPro: 1.312 ± 0.05	1.808GlyGln: 1.808 ± 0.055	1.838GlyArg: 1.838 ± 0.06	4.012GlySer: 4.012 ± 0.094	4.303GlyThr: 4.303 ± 0.142	4.237GlyVal: 4.237 ± 0.082	0.691GlyTrp: 0.691 ± 0.034	2.68GlyTyr: 2.68 ± 0.057	0.0GlyXaa: 0.0 ± 0.0
His	0.883HisAla: 0.883 ± 0.035	0.165HisCys: 0.165 ± 0.015	0.796HisAsp: 0.796 ± 0.033	0.957HisGlu: 0.957 ± 0.038	1.207HisPhe: 1.207 ± 0.043	0.917HisGly: 0.917 ± 0.036	0.449HisHis: 0.449 ± 0.025	1.328HisIle: 1.328 ± 0.036	1.077HisLys: 1.077 ± 0.042	1.596HisLeu: 1.596 ± 0.044	0.315HisMet: 0.315 ± 0.018	0.951HisAsn: 0.951 ± 0.03	0.785HisPro: 0.785 ± 0.037	0.671HisGln: 0.671 ± 0.027	0.554HisArg: 0.554 ± 0.025	0.987HisSer: 0.987 ± 0.041	0.801HisThr: 0.801 ± 0.028	0.921HisVal: 0.921 ± 0.036	0.193HisTrp: 0.193 ± 0.014	0.785HisTyr: 0.785 ± 0.032	0.0HisXaa: 0.0 ± 0.0
Ile	5.643IleAla: 5.643 ± 0.084	0.665IleCys: 0.665 ± 0.033	4.862IleAsp: 4.862 ± 0.076	5.907IleGlu: 5.907 ± 0.096	3.919IlePhe: 3.919 ± 0.082	5.266IleGly: 5.266 ± 0.095	1.362IleHis: 1.362 ± 0.046	6.713IleIle: 6.713 ± 0.122	6.261IleLys: 6.261 ± 0.112	7.146IleLeu: 7.146 ± 0.118	1.454IleMet: 1.454 ± 0.045	5.128IleAsn: 5.128 ± 0.106	3.2IlePro: 3.2 ± 0.066	2.933IleGln: 2.933 ± 0.064	2.379IleArg: 2.379 ± 0.06	6.167IleSer: 6.167 ± 0.117	5.259IleThr: 5.259 ± 0.104	5.189IleVal: 5.189 ± 0.083	0.704IleTrp: 0.704 ± 0.035	2.991IleTyr: 2.991 ± 0.066	0.0IleXaa: 0.0 ± 0.0
Lys	4.952LysAla: 4.952 ± 0.095	0.395LysCys: 0.395 ± 0.023	4.042LysAsp: 4.042 ± 0.086	6.286LysGlu: 6.286 ± 0.101	3.275LysPhe: 3.275 ± 0.068	4.401LysGly: 4.401 ± 0.088	1.239LysHis: 1.239 ± 0.041	7.275LysIle: 7.275 ± 0.114	7.489LysLys: 7.489 ± 0.118	6.731LysLeu: 6.731 ± 0.112	2.387LysMet: 2.387 ± 0.066	5.909LysAsn: 5.909 ± 0.1	2.443LysPro: 2.443 ± 0.057	2.752LysGln: 2.752 ± 0.063	2.566LysArg: 2.566 ± 0.062	4.7LysSer: 4.7 ± 0.081	4.67LysThr: 4.67 ± 0.081	4.964LysVal: 4.964 ± 0.086	0.748LysTrp: 0.748 ± 0.029	3.224LysTyr: 3.224 ± 0.069	0.0LysXaa: 0.0 ± 0.0
Leu	5.541LeuAla: 5.541 ± 0.087	0.598LeuCys: 0.598 ± 0.024	4.777LeuAsp: 4.777 ± 0.085	5.882LeuGlu: 5.882 ± 0.106	5.304LeuPhe: 5.304 ± 0.104	5.472LeuGly: 5.472 ± 0.094	1.556LeuHis: 1.556 ± 0.048	6.869LeuIle: 6.869 ± 0.112	7.459LeuLys: 7.459 ± 0.115	8.455LeuLeu: 8.455 ± 0.128	1.91LeuMet: 1.91 ± 0.049	5.751LeuAsn: 5.751 ± 0.107	3.35LeuPro: 3.35 ± 0.069	3.213LeuGln: 3.213 ± 0.07	2.781LeuArg: 2.781 ± 0.061	6.382LeuSer: 6.382 ± 0.097	4.924LeuThr: 4.924 ± 0.085	5.521LeuVal: 5.521 ± 0.087	0.698LeuTrp: 0.698 ± 0.033	3.116LeuTyr: 3.116 ± 0.059	0.0LeuXaa: 0.0 ± 0.0
Met	1.516MetAla: 1.516 ± 0.045	0.141MetCys: 0.141 ± 0.013	1.001MetAsp: 1.001 ± 0.035	1.422MetGlu: 1.422 ± 0.044	0.961MetPhe: 0.961 ± 0.044	1.398MetGly: 1.398 ± 0.038	0.38MetHis: 0.38 ± 0.025	1.65MetIle: 1.65 ± 0.049	2.322MetLys: 2.322 ± 0.057	1.888MetLeu: 1.888 ± 0.051	0.641MetMet: 0.641 ± 0.035	1.447MetAsn: 1.447 ± 0.043	0.815MetPro: 0.815 ± 0.032	0.9MetGln: 0.9 ± 0.031	0.791MetArg: 0.791 ± 0.033	1.449MetSer: 1.449 ± 0.045	1.178MetThr: 1.178 ± 0.042	1.374MetVal: 1.374 ± 0.047	0.171MetTrp: 0.171 ± 0.014	0.771MetTyr: 0.771 ± 0.03	0.0MetXaa: 0.0 ± 0.0
Asn	3.831AsnAla: 3.831 ± 0.076	0.549AsnCys: 0.549 ± 0.027	3.331AsnAsp: 3.331 ± 0.071	4.475AsnGlu: 4.475 ± 0.08	3.549AsnPhe: 3.549 ± 0.076	4.381AsnGly: 4.381 ± 0.114	1.13AsnHis: 1.13 ± 0.041	4.997AsnIle: 4.997 ± 0.09	4.842AsnLys: 4.842 ± 0.083	5.735AsnLeu: 5.735 ± 0.096	1.34AsnMet: 1.34 ± 0.041	4.474AsnAsn: 4.474 ± 0.101	3.051AsnPro: 3.051 ± 0.068	2.738AsnGln: 2.738 ± 0.064	2.052AsnArg: 2.052 ± 0.057	4.401AsnSer: 4.401 ± 0.09	3.783AsnThr: 3.783 ± 0.097	3.77AsnVal: 3.77 ± 0.069	0.935AsnTrp: 0.935 ± 0.039	3.265AsnTyr: 3.265 ± 0.07	0.0AsnXaa: 0.0 ± 0.0
Pro	2.013ProAla: 2.013 ± 0.074	0.198ProCys: 0.198 ± 0.015	1.631ProAsp: 1.631 ± 0.047	2.552ProGlu: 2.552 ± 0.054	1.952ProPhe: 1.952 ± 0.05	1.788ProGly: 1.788 ± 0.048	0.525ProHis: 0.525 ± 0.027	2.85ProIle: 2.85 ± 0.061	2.552ProLys: 2.552 ± 0.063	2.599ProLeu: 2.599 ± 0.057	0.697ProMet: 0.697 ± 0.029	2.468ProAsn: 2.468 ± 0.061	0.666ProPro: 0.666 ± 0.032	1.005ProGln: 1.005 ± 0.038	0.812ProArg: 0.812 ± 0.032	2.234ProSer: 2.234 ± 0.052	2.294ProThr: 2.294 ± 0.105	2.191ProVal: 2.191 ± 0.066	0.259ProTrp: 0.259 ± 0.016	1.317ProTyr: 1.317 ± 0.044	0.0ProXaa: 0.0 ± 0.0
Gln	1.884GlnAla: 1.884 ± 0.049	0.203GlnCys: 0.203 ± 0.019	1.623GlnAsp: 1.623 ± 0.046	2.289GlnGlu: 2.289 ± 0.053	1.91GlnPhe: 1.91 ± 0.046	1.687GlnGly: 1.687 ± 0.047	0.575GlnHis: 0.575 ± 0.029	2.906GlnIle: 2.906 ± 0.053	2.983GlnLys: 2.983 ± 0.071	3.471GlnLeu: 3.471 ± 0.07	0.917GlnMet: 0.917 ± 0.033	2.58GlnAsn: 2.58 ± 0.06	1.021GlnPro: 1.021 ± 0.036	1.298GlnGln: 1.298 ± 0.046	0.981GlnArg: 0.981 ± 0.034	1.937GlnSer: 1.937 ± 0.045	1.948GlnThr: 1.948 ± 0.051	1.937GlnVal: 1.937 ± 0.05	0.316GlnTrp: 0.316 ± 0.021	1.384GlnTyr: 1.384 ± 0.05	0.0GlnXaa: 0.0 ± 0.0
Arg	1.814ArgAla: 1.814 ± 0.05	0.173ArgCys: 0.173 ± 0.015	1.546ArgAsp: 1.546 ± 0.048	1.911ArgGlu: 1.911 ± 0.056	1.782ArgPhe: 1.782 ± 0.048	1.733ArgGly: 1.733 ± 0.048	0.504ArgHis: 0.504 ± 0.027	2.625ArgIle: 2.625 ± 0.066	2.577ArgLys: 2.577 ± 0.061	2.717ArgLeu: 2.717 ± 0.065	0.823ArgMet: 0.823 ± 0.032	2.046ArgAsn: 2.046 ± 0.053	0.953ArgPro: 0.953 ± 0.04	0.908ArgGln: 0.908 ± 0.033	1.099ArgArg: 1.099 ± 0.044	1.644ArgSer: 1.644 ± 0.051	1.638ArgThr: 1.638 ± 0.046	1.891ArgVal: 1.891 ± 0.047	0.302ArgTrp: 0.302 ± 0.022	1.313ArgTyr: 1.313 ± 0.041	0.0ArgXaa: 0.0 ± 0.0
Ser	3.758SerAla: 3.758 ± 0.105	0.731SerCys: 0.731 ± 0.042	3.495SerAsp: 3.495 ± 0.063	4.097SerGlu: 4.097 ± 0.072	4.067SerPhe: 4.067 ± 0.084	4.716SerGly: 4.716 ± 0.098	1.065SerHis: 1.065 ± 0.038	5.413SerIle: 5.413 ± 0.092	5.15SerLys: 5.15 ± 0.088	5.728SerLeu: 5.728 ± 0.094	1.272SerMet: 1.272 ± 0.039	4.523SerAsn: 4.523 ± 0.11	1.997SerPro: 1.997 ± 0.062	2.146SerGln: 2.146 ± 0.057	1.859SerArg: 1.859 ± 0.055	4.445SerSer: 4.445 ± 0.116	3.689SerThr: 3.689 ± 0.101	4.1SerVal: 4.1 ± 0.1	0.661SerTrp: 0.661 ± 0.031	2.795SerTyr: 2.795 ± 0.057	0.0SerXaa: 0.0 ± 0.0
Thr	3.827ThrAla: 3.827 ± 0.111	0.45ThrCys: 0.45 ± 0.039	2.988ThrAsp: 2.988 ± 0.068	3.403ThrGlu: 3.403 ± 0.068	3.404ThrPhe: 3.404 ± 0.08	4.033ThrGly: 4.033 ± 0.107	0.917ThrHis: 0.917 ± 0.033	5.631ThrIle: 5.631 ± 0.119	4.173ThrLys: 4.173 ± 0.079	5.181ThrLeu: 5.181 ± 0.1	0.983ThrMet: 0.983 ± 0.041	3.821ThrAsn: 3.821 ± 0.091	2.611ThrPro: 2.611 ± 0.096	1.838ThrGln: 1.838 ± 0.04	1.47ThrArg: 1.47 ± 0.046	4.131ThrSer: 4.131 ± 0.121	4.092ThrThr: 4.092 ± 0.14	3.69ThrVal: 3.69 ± 0.126	0.617ThrTrp: 0.617 ± 0.031	2.445ThrTyr: 2.445 ± 0.08	0.0ThrXaa: 0.0 ± 0.0
Val	4.436ValAla: 4.436 ± 0.097	0.54ValCys: 0.54 ± 0.027	3.387ValAsp: 3.387 ± 0.074	3.839ValGlu: 3.839 ± 0.09	3.56ValPhe: 3.56 ± 0.065	3.856ValGly: 3.856 ± 0.078	0.956ValHis: 0.956 ± 0.034	5.122ValIle: 5.122 ± 0.082	4.542ValLys: 4.542 ± 0.083	5.887ValLeu: 5.887 ± 0.1	1.307ValMet: 1.307 ± 0.037	3.772ValAsn: 3.772 ± 0.072	2.078ValPro: 2.078 ± 0.05	1.826ValGln: 1.826 ± 0.052	1.866ValArg: 1.866 ± 0.047	4.417ValSer: 4.417 ± 0.093	3.92ValThr: 3.92 ± 0.118	4.469ValVal: 4.469 ± 0.099	0.547ValTrp: 0.547 ± 0.025	2.392ValTyr: 2.392 ± 0.062	0.0ValXaa: 0.0 ± 0.0
Trp	0.563TrpAla: 0.563 ± 0.028	0.1TrpCys: 0.1 ± 0.011	0.565TrpAsp: 0.565 ± 0.031	0.628TrpGlu: 0.628 ± 0.028	0.583TrpPhe: 0.583 ± 0.025	0.598TrpGly: 0.598 ± 0.036	0.209TrpHis: 0.209 ± 0.016	0.787TrpIle: 0.787 ± 0.033	0.802TrpLys: 0.802 ± 0.032	0.886TrpLeu: 0.886 ± 0.033	0.281TrpMet: 0.281 ± 0.018	0.836TrpAsn: 0.836 ± 0.035	0.191TrpPro: 0.191 ± 0.018	0.385TrpGln: 0.385 ± 0.027	0.339TrpArg: 0.339 ± 0.023	0.589TrpSer: 0.589 ± 0.032	0.532TrpThr: 0.532 ± 0.027	0.559TrpVal: 0.559 ± 0.026	0.122TrpTrp: 0.122 ± 0.013	0.427TrpTyr: 0.427 ± 0.028	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.329TyrAla: 2.329 ± 0.06	0.381TyrCys: 0.381 ± 0.021	2.309TyrAsp: 2.309 ± 0.062	2.345TyrGlu: 2.345 ± 0.06	2.778TyrPhe: 2.778 ± 0.068	2.48TyrGly: 2.48 ± 0.054	0.767TyrHis: 0.767 ± 0.032	2.845TyrIle: 2.845 ± 0.059	3.124TyrLys: 3.124 ± 0.066	3.764TyrLeu: 3.764 ± 0.062	0.749TyrMet: 0.749 ± 0.031	2.76TyrAsn: 2.76 ± 0.068	1.397TyrPro: 1.397 ± 0.04	1.553TyrGln: 1.553 ± 0.047	1.358TyrArg: 1.358 ± 0.036	2.773TyrSer: 2.773 ± 0.066	2.352TyrThr: 2.352 ± 0.082	2.323TyrVal: 2.323 ± 0.061	0.481TyrTrp: 0.481 ± 0.022	1.894TyrTyr: 1.894 ± 0.052	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2588 proteins (844067 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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