Mungbean yellow mosaic virus (strain Vigna) (MYMV)
Average proteome isoelectric point is 7.74
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|Q9YPS7|MVP_MYMVV Movement protein BC1 OS=Mungbean yellow mosaic virus (strain Vigna) OX=223295 GN=BC1 PE=3 SV=1
MM1 pKa = 8.03 WDD3 pKa = 3.53 PLVNDD8 pKa = 4.95 FPKK11 pKa = 10.42 SLHH14 pKa = 6.08 GFRR17 pKa = 11.84 CMLAIKK23 pKa = 9.24 YY24 pKa = 8.3 LQYY27 pKa = 10.48 IQEE30 pKa = 4.51 NYY32 pKa = 9.5 PSNSLGFVYY41 pKa = 9.17 LTEE44 pKa = 5.51 LIQVLRR50 pKa = 11.84 IRR52 pKa = 11.84 KK53 pKa = 8.14 HH54 pKa = 5.7 AKK56 pKa = 9.43 AEE58 pKa = 3.7 LRR60 pKa = 11.84 YY61 pKa = 9.62 RR62 pKa = 11.84 LLYY65 pKa = 10.08 PDD67 pKa = 4.13 VEE69 pKa = 4.62 CAEE72 pKa = 4.5 EE73 pKa = 4.23 ADD75 pKa = 3.82 LRR77 pKa = 11.84 HH78 pKa = 6.2 PAFLTCHH85 pKa = 6.56 CGKK88 pKa = 10.35 CPCQRR93 pKa = 11.84 EE94 pKa = 4.33 KK95 pKa = 11.56 EE96 pKa = 4.34 EE97 pKa = 4.13 VDD99 pKa = 3.42 QPTHH103 pKa = 6.8 VEE105 pKa = 3.86 EE106 pKa = 4.39 TEE108 pKa = 4.0 ILSVIPLSS116 pKa = 3.7
Molecular weight: 13.59 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.348
IPC2_protein 5.512
IPC_protein 5.474
Toseland 5.741
ProMoST 5.753
Dawson 5.664
Bjellqvist 5.69
Wikipedia 5.626
Rodwell 5.639
Grimsley 5.83
Solomon 5.664
Lehninger 5.639
Nozaki 5.893
DTASelect 6.059
Thurlkill 6.033
EMBOSS 5.982
Sillero 5.995
Patrickios 3.3
IPC_peptide 5.677
IPC2_peptide 6.008
IPC2.peptide.svr19 5.992
Protein with the highest isoelectric point:
>sp|Q9YPS6|AV2_MYMVV Protein AV2 OS=Mungbean yellow mosaic virus (strain Vigna) OX=223295 GN=AV2 PE=3 SV=1
MM1 pKa = 8.06 PKK3 pKa = 10.14 RR4 pKa = 11.84 NYY6 pKa = 8.53 DD7 pKa = 3.7 TAFSTPMSNVRR18 pKa = 11.84 RR19 pKa = 11.84 RR20 pKa = 11.84 LTFDD24 pKa = 3.33 TPLSLPATAGSVPASAKK41 pKa = 9.31 RR42 pKa = 11.84 RR43 pKa = 11.84 RR44 pKa = 11.84 WTNRR48 pKa = 11.84 PMWRR52 pKa = 11.84 KK53 pKa = 7.82 PRR55 pKa = 11.84 YY56 pKa = 7.63 YY57 pKa = 10.29 RR58 pKa = 11.84 LYY60 pKa = 10.24 RR61 pKa = 11.84 SPDD64 pKa = 3.2 VPRR67 pKa = 11.84 GCEE70 pKa = 4.34 GPCKK74 pKa = 10.07 VQSFEE79 pKa = 4.11 AKK81 pKa = 9.93 HH82 pKa = 6.44 DD83 pKa = 3.51 ISHH86 pKa = 5.76 VGKK89 pKa = 10.03 VICVTDD95 pKa = 3.22 VTRR98 pKa = 11.84 GMGITHH104 pKa = 6.89 RR105 pKa = 11.84 VGKK108 pKa = 9.5 RR109 pKa = 11.84 FCVKK113 pKa = 10.5 SIWVTGKK120 pKa = 9.22 IWMDD124 pKa = 3.31 EE125 pKa = 4.07 NIKK128 pKa = 9.32 TKK130 pKa = 10.61 NHH132 pKa = 6.01 TNTVMFKK139 pKa = 10.36 LVRR142 pKa = 11.84 DD143 pKa = 3.79 RR144 pKa = 11.84 RR145 pKa = 11.84 PFGTPQDD152 pKa = 3.81 FGQVFNMYY160 pKa = 10.66 DD161 pKa = 3.86 NEE163 pKa = 4.34 PSTATVKK170 pKa = 10.81 NDD172 pKa = 2.84 LRR174 pKa = 11.84 DD175 pKa = 3.4 RR176 pKa = 11.84 YY177 pKa = 9.76 QVVRR181 pKa = 11.84 KK182 pKa = 8.4 FQATVTGGQYY192 pKa = 10.83 ASKK195 pKa = 9.91 EE196 pKa = 3.76 QAIVSKK202 pKa = 9.71 FYY204 pKa = 10.67 RR205 pKa = 11.84 VNNYY209 pKa = 7.79 VVYY212 pKa = 10.46 NHH214 pKa = 6.03 QEE216 pKa = 3.42 AAKK219 pKa = 10.4 YY220 pKa = 9.25 EE221 pKa = 4.13 NHH223 pKa = 6.46 TEE225 pKa = 3.99 NALLLYY231 pKa = 7.29 MACTHH236 pKa = 7.07 ASNPVYY242 pKa = 9.86 ATLKK246 pKa = 9.47 IRR248 pKa = 11.84 IYY250 pKa = 10.59 FYY252 pKa = 11.32 DD253 pKa = 4.06 SISNN257 pKa = 3.66
Molecular weight: 29.83 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.267
IPC2_protein 9.531
IPC_protein 9.736
Toseland 10.028
ProMoST 9.794
Dawson 10.277
Bjellqvist 9.984
Wikipedia 10.467
Rodwell 10.584
Grimsley 10.35
Solomon 10.292
Lehninger 10.248
Nozaki 10.058
DTASelect 9.97
Thurlkill 10.116
EMBOSS 10.452
Sillero 10.189
Patrickios 9.882
IPC_peptide 10.292
IPC2_peptide 8.858
IPC2.peptide.svr19 8.459
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8
0
8
1656
99
361
207.0
23.67
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.374 ± 0.573
2.053 ± 0.307
5.435 ± 0.556
4.589 ± 0.648
4.227 ± 0.237
4.891 ± 0.615
3.382 ± 0.394
5.193 ± 0.559
6.824 ± 0.331
8.213 ± 1.079
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.717 ± 0.534
5.616 ± 0.389
5.918 ± 0.459
3.684 ± 0.245
6.159 ± 0.644
8.816 ± 0.814
5.495 ± 0.851
5.676 ± 0.586
1.208 ± 0.118
4.529 ± 0.305
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here