Candidatus Photodesmus blepharus
Average proteome isoelectric point is 7.34
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 923 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A084CPI7|A0A084CPI7_9GAMM Lipoyl synthase OS=Candidatus Photodesmus blepharus OX=1179155 GN=lipA PE=3 SV=1
MM1 pKa = 7.7 PKK3 pKa = 9.91 IIVLPHH9 pKa = 6.65 SEE11 pKa = 4.53 LCPEE15 pKa = 4.39 GAVLEE20 pKa = 4.6 AKK22 pKa = 10.33 SGEE25 pKa = 4.45 SILDD29 pKa = 3.42 VALEE33 pKa = 3.84 NAIDD37 pKa = 3.87 IPHH40 pKa = 6.79 ACEE43 pKa = 4.08 KK44 pKa = 10.85 SCACTTCHH52 pKa = 5.75 IVIRR56 pKa = 11.84 EE57 pKa = 4.21 GFDD60 pKa = 3.08 SLEE63 pKa = 4.16 ISDD66 pKa = 4.44 EE67 pKa = 4.52 LEE69 pKa = 5.4 DD70 pKa = 5.7 DD71 pKa = 3.73 MLDD74 pKa = 3.71 RR75 pKa = 11.84 AWGLEE80 pKa = 3.86 PEE82 pKa = 4.65 SRR84 pKa = 11.84 LSCQAKK90 pKa = 10.3 VLDD93 pKa = 4.2 EE94 pKa = 4.7 DD95 pKa = 4.69 LVIEE99 pKa = 4.19 IPKK102 pKa = 8.65 YY103 pKa = 7.63 TVNYY107 pKa = 10.32 VLDD110 pKa = 4.06 NYY112 pKa = 11.39
Molecular weight: 12.45 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.907
IPC2_protein 4.19
IPC_protein 4.113
Toseland 3.935
ProMoST 4.228
Dawson 4.062
Bjellqvist 4.215
Wikipedia 3.948
Rodwell 3.948
Grimsley 3.846
Solomon 4.062
Lehninger 4.012
Nozaki 4.177
DTASelect 4.329
Thurlkill 3.961
EMBOSS 3.961
Sillero 4.215
Patrickios 1.939
IPC_peptide 4.062
IPC2_peptide 4.202
IPC2.peptide.svr19 4.12
Protein with the highest isoelectric point:
>tr|A0A084CNQ7|A0A084CNQ7_9GAMM Protein HflK OS=Candidatus Photodesmus blepharus OX=1179155 GN=hflK PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.5 RR3 pKa = 11.84 TFQPTVLKK11 pKa = 10.55 RR12 pKa = 11.84 SRR14 pKa = 11.84 THH16 pKa = 6.4 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.36 NGRR28 pKa = 11.84 KK29 pKa = 9.19 IINARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.5 GRR39 pKa = 11.84 IRR41 pKa = 11.84 LSKK44 pKa = 10.72
Molecular weight: 5.2 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.213
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.676
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.969
DTASelect 12.954
Thurlkill 12.969
EMBOSS 13.466
Sillero 12.969
Patrickios 12.398
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.148
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
923
0
923
307214
37
1615
332.8
37.46
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.53 ± 0.067
1.166 ± 0.03
4.86 ± 0.05
6.149 ± 0.074
4.395 ± 0.062
6.296 ± 0.067
2.075 ± 0.037
8.544 ± 0.063
7.056 ± 0.077
10.291 ± 0.082
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.508 ± 0.032
5.174 ± 0.056
3.34 ± 0.034
3.78 ± 0.055
5.002 ± 0.058
7.083 ± 0.059
4.854 ± 0.045
6.757 ± 0.078
1.03 ± 0.029
3.111 ± 0.042
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here