Heliothis zea nudivirus
Average proteome isoelectric point is 7.17
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 154 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q8JKG6|Q8JKG6_9VIRU Orf147 OS=Heliothis zea nudivirus OX=29250 GN=orf147 PE=4 SV=1
MM1 pKa = 7.44 SLLRR5 pKa = 11.84 KK6 pKa = 7.25 PTSEE10 pKa = 4.23 TEE12 pKa = 3.81 FDD14 pKa = 4.94 LEE16 pKa = 4.72 DD17 pKa = 3.61 LTISLSDD24 pKa = 3.68 YY25 pKa = 10.3 PLSNHH30 pKa = 6.83 SIVDD34 pKa = 4.69 DD35 pKa = 3.95 IQHH38 pKa = 6.69 LMGDD42 pKa = 4.81 LYY44 pKa = 10.54 YY45 pKa = 10.62 QCNDD49 pKa = 3.09 STCRR53 pKa = 11.84 CKK55 pKa = 10.41 IEE57 pKa = 4.5 IIVGFLMGFILGSVLARR74 pKa = 11.84 LTSGYY79 pKa = 10.15 AANAHH84 pKa = 6.88 HH85 pKa = 6.75 SRR87 pKa = 11.84 QDD89 pKa = 3.27 QTDD92 pKa = 3.64 DD93 pKa = 3.66 EE94 pKa = 4.85 GLGTSTEE101 pKa = 4.11 EE102 pKa = 5.15 GEE104 pKa = 4.88 NADD107 pKa = 5.8 DD108 pKa = 6.37 DD109 pKa = 5.38 DD110 pKa = 6.87 DD111 pKa = 6.07 DD112 pKa = 5.1 YY113 pKa = 12.16 SSSSEE118 pKa = 3.83 EE119 pKa = 3.8 ATDD122 pKa = 3.74 YY123 pKa = 11.23 GASTDD128 pKa = 4.19 EE129 pKa = 5.32 DD130 pKa = 5.07 DD131 pKa = 5.45 DD132 pKa = 5.73 DD133 pKa = 4.97 EE134 pKa = 5.47 SASEE138 pKa = 4.0 WGNEE142 pKa = 3.84 VHH144 pKa = 7.39 LDD146 pKa = 3.4
Molecular weight: 16.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.724
IPC2_protein 3.668
IPC_protein 3.668
Toseland 3.452
ProMoST 3.808
Dawson 3.668
Bjellqvist 3.859
Wikipedia 3.617
Rodwell 3.503
Grimsley 3.363
Solomon 3.656
Lehninger 3.617
Nozaki 3.783
DTASelect 4.037
Thurlkill 3.516
EMBOSS 3.63
Sillero 3.795
Patrickios 0.769
IPC_peptide 3.656
IPC2_peptide 3.77
IPC2.peptide.svr19 3.732
Protein with the highest isoelectric point:
>tr|Q8JKS1|Q8JKS1_9VIRU Arginine-rich protein a209R OS=Heliothis zea nudivirus OX=29250 GN=orf40 PE=4 SV=1
MM1 pKa = 7.5 RR2 pKa = 11.84 TPLNVKK8 pKa = 9.63 PRR10 pKa = 11.84 LKK12 pKa = 10.12 QQSAKK17 pKa = 10.1 RR18 pKa = 11.84 RR19 pKa = 11.84 LKK21 pKa = 10.52 LLPNVKK27 pKa = 9.54 PRR29 pKa = 11.84 LKK31 pKa = 10.18 RR32 pKa = 11.84 QPNSKK37 pKa = 9.86 RR38 pKa = 11.84 RR39 pKa = 11.84 LKK41 pKa = 10.71 LPPNVKK47 pKa = 9.65 PRR49 pKa = 11.84 LKK51 pKa = 10.18 RR52 pKa = 11.84 QPNSKK57 pKa = 9.94 RR58 pKa = 11.84 RR59 pKa = 11.84 LKK61 pKa = 11.0 LPLNAKK67 pKa = 9.87 RR68 pKa = 11.84 RR69 pKa = 11.84 LKK71 pKa = 10.16 QPKK74 pKa = 9.37 PNVKK78 pKa = 9.9 LRR80 pKa = 11.84 LKK82 pKa = 10.28 QPPNVKK88 pKa = 9.78 RR89 pKa = 11.84 RR90 pKa = 11.84 LKK92 pKa = 10.64 LPPNAKK98 pKa = 9.64 RR99 pKa = 11.84 RR100 pKa = 11.84 LKK102 pKa = 10.95 LPLNAKK108 pKa = 9.85 RR109 pKa = 11.84 RR110 pKa = 11.84 LKK112 pKa = 10.91 LPLNVKK118 pKa = 9.75 PRR120 pKa = 11.84 LKK122 pKa = 10.1 QQNEE126 pKa = 3.91 RR127 pKa = 11.84 PMLKK131 pKa = 9.89 QPPNVKK137 pKa = 10.02 LRR139 pKa = 11.84 LRR141 pKa = 11.84 QQNEE145 pKa = 3.79 RR146 pKa = 11.84 PMLKK150 pKa = 9.89 QPPNVKK156 pKa = 10.02 LRR158 pKa = 11.84 LRR160 pKa = 11.84 QPNAKK165 pKa = 9.62 RR166 pKa = 11.84 RR167 pKa = 11.84 LKK169 pKa = 10.64 LLNEE173 pKa = 5.04 KK174 pKa = 9.26 EE175 pKa = 4.14 LQMNN179 pKa = 4.47
Molecular weight: 21.58 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.448
IPC2_protein 10.979
IPC_protein 12.544
Toseland 12.72
ProMoST 13.203
Dawson 12.72
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.559
Grimsley 12.764
Solomon 13.203
Lehninger 13.1
Nozaki 12.72
DTASelect 12.705
Thurlkill 12.72
EMBOSS 13.217
Sillero 12.72
Patrickios 12.266
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.038
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
154
0
154
52489
44
1585
340.8
38.52
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.152 ± 0.152
2.103 ± 0.121
6.338 ± 0.189
5.715 ± 0.207
3.742 ± 0.13
4.241 ± 0.126
2.351 ± 0.106
5.546 ± 0.13
6.716 ± 0.216
8.329 ± 0.307
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.705 ± 0.101
6.958 ± 0.283
4.494 ± 0.227
3.241 ± 0.121
4.851 ± 0.162
9.048 ± 0.378
7.278 ± 0.347
6.542 ± 0.159
0.583 ± 0.051
4.048 ± 0.173
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here