Cow vetch latent virus
Average proteome isoelectric point is 6.83
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2H4T2E8|A0A2H4T2E8_9VIRU Cell cycle link protein OS=Cow vetch latent virus OX=2056780 GN=clink PE=4 SV=1
MM1 pKa = 6.46 VLKK4 pKa = 10.54 YY5 pKa = 10.18 FSLLPSEE12 pKa = 4.55 LKK14 pKa = 10.02 EE15 pKa = 4.23 KK16 pKa = 10.32 IVRR19 pKa = 11.84 EE20 pKa = 3.91 HH21 pKa = 6.38 LKK23 pKa = 10.53 EE24 pKa = 3.73 EE25 pKa = 4.22 RR26 pKa = 11.84 KK27 pKa = 9.81 RR28 pKa = 11.84 EE29 pKa = 3.85 FLEE32 pKa = 4.01 NSVLAACRR40 pKa = 11.84 RR41 pKa = 11.84 YY42 pKa = 10.41 EE43 pKa = 4.55 DD44 pKa = 5.45 LLLKK48 pKa = 10.61 DD49 pKa = 5.48 DD50 pKa = 4.09 ITDD53 pKa = 3.51 EE54 pKa = 4.27 EE55 pKa = 4.78 LLHH58 pKa = 6.52 LSKK61 pKa = 10.77 FLDD64 pKa = 3.53 SLVQYY69 pKa = 8.42 VTDD72 pKa = 4.39 YY73 pKa = 10.89 IGSRR77 pKa = 11.84 CLIRR81 pKa = 11.84 WKK83 pKa = 10.49 KK84 pKa = 10.41 DD85 pKa = 2.85 VSPRR89 pKa = 11.84 VKK91 pKa = 10.16 YY92 pKa = 10.76 SVMEE96 pKa = 3.98 EE97 pKa = 3.77 QHH99 pKa = 5.23 IQLYY103 pKa = 9.12 GFRR106 pKa = 11.84 DD107 pKa = 3.79 MEE109 pKa = 4.49 DD110 pKa = 3.73 LSCEE114 pKa = 3.99 EE115 pKa = 4.22 LLLPEE120 pKa = 4.18 VEE122 pKa = 4.17 EE123 pKa = 5.38 DD124 pKa = 2.86 ITYY127 pKa = 10.26 EE128 pKa = 3.95 DD129 pKa = 4.43 GMIVNCGQLDD139 pKa = 3.68 ALFRR143 pKa = 11.84 DD144 pKa = 3.94 LGVTVVYY151 pKa = 8.92 ITIGNGLILLPMCKK165 pKa = 9.84 EE166 pKa = 3.9 IVITT170 pKa = 4.5
Molecular weight: 19.93 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.686
IPC2_protein 4.736
IPC_protein 4.647
Toseland 4.507
ProMoST 4.736
Dawson 4.584
Bjellqvist 4.736
Wikipedia 4.431
Rodwell 4.495
Grimsley 4.418
Solomon 4.584
Lehninger 4.533
Nozaki 4.685
DTASelect 4.825
Thurlkill 4.495
EMBOSS 4.457
Sillero 4.762
Patrickios 3.757
IPC_peptide 4.596
IPC2_peptide 4.749
IPC2.peptide.svr19 4.743
Protein with the highest isoelectric point:
>tr|A0A2H4T2E3|A0A2H4T2E3_9VIRU U4 protein OS=Cow vetch latent virus OX=2056780 GN=U4 PE=4 SV=1
MM1 pKa = 6.96 VSNWSSSGRR10 pKa = 11.84 KK11 pKa = 6.9 WRR13 pKa = 11.84 HH14 pKa = 4.79 SGRR17 pKa = 11.84 KK18 pKa = 9.11 GGGRR22 pKa = 11.84 PFKK25 pKa = 10.36 PIVPTTRR32 pKa = 11.84 LVVHH36 pKa = 5.97 STALLNKK43 pKa = 9.63 DD44 pKa = 3.92 DD45 pKa = 4.44 VVGCEE50 pKa = 4.49 IRR52 pKa = 11.84 PKK54 pKa = 10.58 DD55 pKa = 3.1 ISRR58 pKa = 11.84 YY59 pKa = 8.95 KK60 pKa = 8.73 MRR62 pKa = 11.84 KK63 pKa = 9.02 VMLTCTVRR71 pKa = 11.84 MPPGEE76 pKa = 4.21 LLNYY80 pKa = 10.26 LVVKK84 pKa = 10.6 CSSPLVNWGAAFSSPALLVKK104 pKa = 10.28 EE105 pKa = 5.02 DD106 pKa = 4.18 CQDD109 pKa = 3.48 MITILGKK116 pKa = 10.87 GKK118 pKa = 10.06 IEE120 pKa = 4.13 SSGVAGSDD128 pKa = 3.44 CTKK131 pKa = 10.46 SFSRR135 pKa = 11.84 FVKK138 pKa = 10.28 LGSGVTQTQHH148 pKa = 7.01 LYY150 pKa = 10.12 VILYY154 pKa = 8.22 TSVSMKK160 pKa = 10.43 SVLEE164 pKa = 3.56 HH165 pKa = 6.12 RR166 pKa = 11.84 MYY168 pKa = 11.42 VEE170 pKa = 4.06 VV171 pKa = 4.09
Molecular weight: 18.94 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.112
IPC2_protein 9.165
IPC_protein 9.121
Toseland 9.97
ProMoST 9.604
Dawson 10.145
Bjellqvist 9.823
Wikipedia 10.277
Rodwell 10.643
Grimsley 10.189
Solomon 10.175
Lehninger 10.145
Nozaki 10.043
DTASelect 9.78
Thurlkill 10.014
EMBOSS 10.365
Sillero 10.087
Patrickios 10.321
IPC_peptide 10.175
IPC2_peptide 8.624
IPC2.peptide.svr19 8.025
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8
0
8
1269
104
286
158.6
18.28
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.546 ± 0.561
2.837 ± 0.484
5.516 ± 0.498
6.619 ± 1.036
4.098 ± 0.471
6.147 ± 0.728
1.655 ± 0.208
5.91 ± 0.696
6.225 ± 0.493
10.402 ± 1.292
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.861 ± 0.249
3.783 ± 0.587
3.388 ± 0.589
3.783 ± 0.483
6.698 ± 0.71
7.092 ± 0.95
4.019 ± 0.478
8.668 ± 0.988
1.497 ± 0.24
4.255 ± 0.372
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here