Pseudoxanthomonas composti
Average proteome isoelectric point is 6.73
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3624 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4Q1JRI1|A0A4Q1JRI1_9GAMM Uncharacterized protein OS=Pseudoxanthomonas composti OX=2137479 GN=EPA99_17125 PE=4 SV=1
MM1 pKa = 6.99 QVAPYY6 pKa = 10.79 LFFNGDD12 pKa = 3.41 CRR14 pKa = 11.84 QAMQFYY20 pKa = 10.78 ADD22 pKa = 3.76 MLGGQLVALSTVDD35 pKa = 3.53 QAPPDD40 pKa = 3.45 ARR42 pKa = 11.84 MPGMPDD48 pKa = 3.4 DD49 pKa = 4.51 SVMHH53 pKa = 6.22 AMVVAGDD60 pKa = 3.86 VTLMASDD67 pKa = 4.35 ACPPQDD73 pKa = 3.55 YY74 pKa = 8.57 TPPHH78 pKa = 6.53 GMTVSLQVDD87 pKa = 3.66 TVEE90 pKa = 4.15 QAEE93 pKa = 4.19 RR94 pKa = 11.84 LYY96 pKa = 11.26 AALAEE101 pKa = 4.57 GAQVQMPLGKK111 pKa = 8.51 TFWSEE116 pKa = 3.51 AFAMLTDD123 pKa = 4.12 RR124 pKa = 11.84 FGTPWMINVVQACGPDD140 pKa = 3.33 ASAA143 pKa = 5.04
Molecular weight: 15.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.745
IPC2_protein 3.923
IPC_protein 3.884
Toseland 3.668
ProMoST 4.075
Dawson 3.897
Bjellqvist 4.062
Wikipedia 3.872
Rodwell 3.719
Grimsley 3.579
Solomon 3.884
Lehninger 3.846
Nozaki 4.024
DTASelect 4.291
Thurlkill 3.745
EMBOSS 3.872
Sillero 4.024
Patrickios 0.896
IPC_peptide 3.884
IPC2_peptide 3.986
IPC2.peptide.svr19 3.894
Protein with the highest isoelectric point:
>tr|A0A4Q1JWA4|A0A4Q1JWA4_9GAMM Uncharacterized protein OS=Pseudoxanthomonas composti OX=2137479 GN=EPA99_08065 PE=4 SV=1
MM1 pKa = 7.44 LKK3 pKa = 10.42 AEE5 pKa = 4.12 HH6 pKa = 6.6 FYY8 pKa = 10.65 WLVGAFLLATAWHH21 pKa = 6.08 NLRR24 pKa = 11.84 RR25 pKa = 11.84 GRR27 pKa = 11.84 NAMAAFWTLLVAPFAFGQAILDD49 pKa = 3.58 AHH51 pKa = 6.91 KK52 pKa = 10.7 AGQALPAQLMGVGVIALGVLAARR75 pKa = 11.84 VRR77 pKa = 11.84 LRR79 pKa = 11.84 AAPDD83 pKa = 3.49 SPDD86 pKa = 3.13 EE87 pKa = 3.9 QLRR90 pKa = 11.84 RR91 pKa = 11.84 KK92 pKa = 10.17 ASASQLGNRR101 pKa = 11.84 LFFPALVIPVMTLLLFVGARR121 pKa = 11.84 WVPGMQGLLDD131 pKa = 4.22 AKK133 pKa = 10.84 ALTLSALALASLVALSAALWVTRR156 pKa = 11.84 AGAGLAAQQGGRR168 pKa = 11.84 LLDD171 pKa = 3.74 ALGWAVVLPMMLATLGGVFAATGIGEE197 pKa = 5.0 LIAALVGKK205 pKa = 8.11 VIPVQSRR212 pKa = 11.84 SACLLAFAGGMVLFTVIMGNAFAAFPVMMAGIGLPLLIQRR252 pKa = 11.84 HH253 pKa = 5.01 GADD256 pKa = 3.76 PASLGAIGMLTGYY269 pKa = 10.05 CGTLLTPMAANFNIVPAILLEE290 pKa = 4.65 LKK292 pKa = 10.29 DD293 pKa = 3.49 QYY295 pKa = 11.88 GVIRR299 pKa = 11.84 MQAATAVVLLVFNIGLMAWVCFRR322 pKa = 4.58
Molecular weight: 33.72 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.355
IPC2_protein 9.575
IPC_protein 10.394
Toseland 10.628
ProMoST 10.584
Dawson 10.73
Bjellqvist 10.452
Wikipedia 10.95
Rodwell 10.891
Grimsley 10.774
Solomon 10.847
Lehninger 10.818
Nozaki 10.643
DTASelect 10.438
Thurlkill 10.628
EMBOSS 11.038
Sillero 10.672
Patrickios 10.643
IPC_peptide 10.862
IPC2_peptide 9.619
IPC2.peptide.svr19 8.702
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3624
0
3624
1257634
30
4077
347.0
37.61
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.237 ± 0.066
0.783 ± 0.013
5.614 ± 0.032
5.147 ± 0.041
3.287 ± 0.029
8.651 ± 0.049
2.174 ± 0.024
3.933 ± 0.028
2.657 ± 0.033
11.042 ± 0.055
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.16 ± 0.02
2.42 ± 0.033
5.399 ± 0.033
4.406 ± 0.029
7.447 ± 0.044
5.368 ± 0.033
5.019 ± 0.036
7.342 ± 0.038
1.549 ± 0.019
2.365 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here