Leuconostoc sp. DORA_2

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae; Leuconostoc; unclassified Leuconostoc

Average proteome isoelectric point is 6.69

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 844 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W1V720|W1V720_9LACO GP-PDE domain-containing protein (Fragment) OS=Leuconostoc sp. DORA_2 OX=1403934 GN=Q611_LSC00217G0001 PE=4 SV=1
DDD2 pKa = 4.08LDDD5 pKa = 5.16SADDD9 pKa = 3.61LTLAQRR15 pKa = 11.84HHH17 pKa = 4.59QAAKKK22 pKa = 10.49DDD24 pKa = 3.91NLLQADDD31 pKa = 3.86RR32 pKa = 11.84DDD34 pKa = 3.18SDDD37 pKa = 2.65PKKK40 pKa = 10.73YYY42 pKa = 9.49DD43 pKa = 3.72IISFADDD50 pKa = 3.2FNYYY54 pKa = 10.46PTLHHH59 pKa = 7.12DD60 pKa = 4.75KKK62 pKa = 11.42ALAQAAQHHH71 pKa = 6.44NTGGQLLFDDD81 pKa = 5.03ITPYYY86 pKa = 10.34VNEEE90 pKa = 4.6YYY92 pKa = 10.35DDD94 pKa = 3.56YY95 pKa = 10.62YY96 pKa = 11.31YY97 pKa = 11.64NDDD100 pKa = 3.87DD101 pKa = 3.91DD102 pKa = 5.58DDD104 pKa = 4.32IFMWTSYYY112 pKa = 10.54GDDD115 pKa = 4.06INSVDDD121 pKa = 3.71HH122 pKa = 6.63DD123 pKa = 4.59KKK125 pKa = 11.28FVYYY129 pKa = 10.57DD130 pKa = 3.58EE131 pKa = 5.55IDDD134 pKa = 3.71FKKK137 pKa = 10.15LRR139 pKa = 11.84EEE141 pKa = 3.79HHH143 pKa = 6.74HH144 pKa = 6.37EE145 pKa = 3.86TYYY148 pKa = 11.3DD149 pKa = 4.19IVYYY153 pKa = 7.7QALEEE158 pKa = 3.93EE159 pKa = 4.47GFKKK163 pKa = 10.28DD164 pKa = 3.95QVMADDD170 pKa = 3.94GDDD173 pKa = 3.88

Molecular weight: 19.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W1V4W8|W1V4W8_9LACO Uncharacterized protein (Fragment) OS=Leuconostoc sp. DORA_2 OX=1403934 GN=Q611_LSC00168G0002 PE=4 SV=1
MM1 pKa = 7.61KK2 pKa = 10.31VRR4 pKa = 11.84NSLRR8 pKa = 11.84ALKK11 pKa = 9.41NQPGSQVVRR20 pKa = 11.84RR21 pKa = 11.84RR22 pKa = 11.84GRR24 pKa = 11.84VYY26 pKa = 10.8VINKK30 pKa = 9.04KK31 pKa = 9.33NPRR34 pKa = 11.84LKK36 pKa = 10.61ARR38 pKa = 11.84QGG40 pKa = 3.23

Molecular weight: 4.67 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

844

0

844

144346

22

840

171.0

19.17

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.803 ± 0.117

0.347 ± 0.029

5.673 ± 0.098

5.182 ± 0.096

4.634 ± 0.077

6.099 ± 0.082

2.152 ± 0.053

7.668 ± 0.104

6.195 ± 0.095

10.012 ± 0.121

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.701 ± 0.051

5.326 ± 0.098

3.163 ± 0.055

4.89 ± 0.085

3.956 ± 0.064

6.025 ± 0.09

6.234 ± 0.087

7.025 ± 0.096

1.088 ± 0.03

3.67 ± 0.067
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski