Boudabousia liubingyangii

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Actinomycetales; Actinomycetaceae; Boudabousia

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1617 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1Q5PQW1|A0A1Q5PQW1_9ACTO Uncharacterized protein OS=Boudabousia liubingyangii OX=1921764 GN=BSR29_02100 PE=4 SV=1
MM1 pKa = 7.72TDD3 pKa = 4.15FVPPFDD9 pKa = 4.82PALQALQNPNLDD21 pKa = 3.85PFVLAQIASDD31 pKa = 5.57RR32 pKa = 11.84PDD34 pKa = 3.08LWSTIVFHH42 pKa = 6.55PAAYY46 pKa = 9.24PEE48 pKa = 4.95LISYY52 pKa = 9.64LEE54 pKa = 4.02QYY56 pKa = 11.27GDD58 pKa = 3.66DD59 pKa = 3.79QVRR62 pKa = 11.84MAVQNRR68 pKa = 11.84RR69 pKa = 11.84NAEE72 pKa = 3.85AQGYY76 pKa = 7.05PLNYY80 pKa = 9.32PYY82 pKa = 10.77AAQPTMPPAPPAPPAAPAVPQYY104 pKa = 8.61PQPAQTFGTAPVRR117 pKa = 11.84RR118 pKa = 11.84QVFGTFEE125 pKa = 4.83HH126 pKa = 6.18NQQTYY131 pKa = 8.02PQPANEE137 pKa = 4.51PAPASYY143 pKa = 9.87EE144 pKa = 3.94AQPVSGEE151 pKa = 3.98PSPVGDD157 pKa = 3.56QVAPAGNEE165 pKa = 3.86FEE167 pKa = 5.62LPANDD172 pKa = 3.69ASQPFNEE179 pKa = 4.66AEE181 pKa = 4.12QPFNEE186 pKa = 4.47AEE188 pKa = 4.19QPVDD192 pKa = 4.36DD193 pKa = 4.36YY194 pKa = 12.11SEE196 pKa = 5.13LEE198 pKa = 3.92ATRR201 pKa = 11.84LVAHH205 pKa = 6.76PRR207 pKa = 11.84YY208 pKa = 10.19EE209 pKa = 4.15NVQALDD215 pKa = 3.68NEE217 pKa = 4.81TEE219 pKa = 3.86ATRR222 pKa = 11.84YY223 pKa = 8.76VPRR226 pKa = 11.84VTLPEE231 pKa = 4.65AEE233 pKa = 4.59GAQAPLPEE241 pKa = 5.74DD242 pKa = 3.65DD243 pKa = 3.96ATRR246 pKa = 11.84LVPRR250 pKa = 11.84VVLPAAEE257 pKa = 4.63DD258 pKa = 3.87GVYY261 pKa = 10.28VPSSVEE267 pKa = 3.64EE268 pKa = 4.4SPAPEE273 pKa = 4.86AEE275 pKa = 4.03AVPEE279 pKa = 4.22PEE281 pKa = 5.06AEE283 pKa = 4.22VAPEE287 pKa = 4.63LEE289 pKa = 4.68PEE291 pKa = 4.23AAPVPEE297 pKa = 5.01PEE299 pKa = 4.57SEE301 pKa = 4.31VAPEE305 pKa = 4.49PEE307 pKa = 4.52PEE309 pKa = 4.04AAPEE313 pKa = 3.84PVLEE317 pKa = 4.48PEE319 pKa = 4.66EE320 pKa = 4.2PASPEE325 pKa = 3.71VDD327 pKa = 3.34VVPEE331 pKa = 4.24PEE333 pKa = 4.37SEE335 pKa = 4.3VAPEE339 pKa = 4.26PEE341 pKa = 4.74PEE343 pKa = 4.35PEE345 pKa = 4.06VAPAPEE351 pKa = 4.59PEE353 pKa = 4.57PEE355 pKa = 4.32SEE357 pKa = 4.58AEE359 pKa = 3.79AAPAEE364 pKa = 4.41GEE366 pKa = 4.27FFVDD370 pKa = 5.37SIPSEE375 pKa = 4.62LNPQDD380 pKa = 4.83GEE382 pKa = 4.44TEE384 pKa = 4.11PDD386 pKa = 3.07HH387 pKa = 6.99GEE389 pKa = 3.9QSSEE393 pKa = 3.93EE394 pKa = 4.24SEE396 pKa = 4.71LEE398 pKa = 3.66SDD400 pKa = 4.15YY401 pKa = 11.88EE402 pKa = 3.99EE403 pKa = 6.19LEE405 pKa = 4.05ATRR408 pKa = 11.84VVPRR412 pKa = 11.84VHH414 pKa = 6.72KK415 pKa = 10.24PFIAPEE421 pKa = 4.04LQQSQGAPAPTPLEE435 pKa = 3.97VDD437 pKa = 3.8AAPAPSPGEE446 pKa = 4.04FEE448 pKa = 4.61AAPAPSPVDD457 pKa = 4.0FEE459 pKa = 4.46PAPAPSPEE467 pKa = 3.9AHH469 pKa = 6.13EE470 pKa = 4.5VAPAPGPEE478 pKa = 4.12DD479 pKa = 5.22LEE481 pKa = 4.49VAPAPSPADD490 pKa = 3.77YY491 pKa = 10.71EE492 pKa = 4.32PAPAPAPTEE501 pKa = 4.1YY502 pKa = 11.05APAPAPNPQYY512 pKa = 10.58EE513 pKa = 4.35PAPAPSGVMPPAFPDD528 pKa = 3.67VPQYY532 pKa = 10.93RR533 pKa = 11.84PNNEE537 pKa = 3.57EE538 pKa = 3.76TSQKK542 pKa = 10.58GSSKK546 pKa = 10.64RR547 pKa = 11.84KK548 pKa = 9.22LLWIILSVIVVIGLCVGGYY567 pKa = 9.45FLYY570 pKa = 10.46SYY572 pKa = 10.19LASGSDD578 pKa = 3.56NEE580 pKa = 4.51SLGNQATSQLQSEE593 pKa = 5.19YY594 pKa = 10.99VPTMYY599 pKa = 10.49FAA601 pKa = 5.55

Molecular weight:
64.31 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1Q5PPC0|A0A1Q5PPC0_9ACTO Uncharacterized protein OS=Boudabousia liubingyangii OX=1921764 GN=BSR29_00315 PE=4 SV=1
MM1 pKa = 7.41VKK3 pKa = 9.03RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 9.4VHH17 pKa = 5.52GFRR20 pKa = 11.84KK21 pKa = 9.95RR22 pKa = 11.84MSTRR26 pKa = 11.84AGRR29 pKa = 11.84AILAARR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.67GRR40 pKa = 11.84ASISAA45 pKa = 3.59

Molecular weight:
5.22 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1617

0

1617

587279

32

3168

363.2

39.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.064 ± 0.087

0.745 ± 0.019

5.289 ± 0.05

6.494 ± 0.059

3.508 ± 0.038

7.876 ± 0.056

1.882 ± 0.023

5.068 ± 0.055

4.335 ± 0.055

10.35 ± 0.076

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.094 ± 0.026

3.201 ± 0.038

5.384 ± 0.06

4.22 ± 0.045

5.46 ± 0.058

5.835 ± 0.042

5.725 ± 0.048

7.505 ± 0.059

1.446 ± 0.026

2.519 ± 0.033

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski