Boudabousia liubingyangii
Average proteome isoelectric point is 6.18
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1617 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1Q5PQW1|A0A1Q5PQW1_9ACTO Uncharacterized protein OS=Boudabousia liubingyangii OX=1921764 GN=BSR29_02100 PE=4 SV=1
MM1 pKa = 7.72 TDD3 pKa = 4.15 FVPPFDD9 pKa = 4.82 PALQALQNPNLDD21 pKa = 3.85 PFVLAQIASDD31 pKa = 5.57 RR32 pKa = 11.84 PDD34 pKa = 3.08 LWSTIVFHH42 pKa = 6.55 PAAYY46 pKa = 9.24 PEE48 pKa = 4.95 LISYY52 pKa = 9.64 LEE54 pKa = 4.02 QYY56 pKa = 11.27 GDD58 pKa = 3.66 DD59 pKa = 3.79 QVRR62 pKa = 11.84 MAVQNRR68 pKa = 11.84 RR69 pKa = 11.84 NAEE72 pKa = 3.85 AQGYY76 pKa = 7.05 PLNYY80 pKa = 9.32 PYY82 pKa = 10.77 AAQPTMPPAPPAPPAAPAVPQYY104 pKa = 8.61 PQPAQTFGTAPVRR117 pKa = 11.84 RR118 pKa = 11.84 QVFGTFEE125 pKa = 4.83 HH126 pKa = 6.18 NQQTYY131 pKa = 8.02 PQPANEE137 pKa = 4.51 PAPASYY143 pKa = 9.87 EE144 pKa = 3.94 AQPVSGEE151 pKa = 3.98 PSPVGDD157 pKa = 3.56 QVAPAGNEE165 pKa = 3.86 FEE167 pKa = 5.62 LPANDD172 pKa = 3.69 ASQPFNEE179 pKa = 4.66 AEE181 pKa = 4.12 QPFNEE186 pKa = 4.47 AEE188 pKa = 4.19 QPVDD192 pKa = 4.36 DD193 pKa = 4.36 YY194 pKa = 12.11 SEE196 pKa = 5.13 LEE198 pKa = 3.92 ATRR201 pKa = 11.84 LVAHH205 pKa = 6.76 PRR207 pKa = 11.84 YY208 pKa = 10.19 EE209 pKa = 4.15 NVQALDD215 pKa = 3.68 NEE217 pKa = 4.81 TEE219 pKa = 3.86 ATRR222 pKa = 11.84 YY223 pKa = 8.76 VPRR226 pKa = 11.84 VTLPEE231 pKa = 4.65 AEE233 pKa = 4.59 GAQAPLPEE241 pKa = 5.74 DD242 pKa = 3.65 DD243 pKa = 3.96 ATRR246 pKa = 11.84 LVPRR250 pKa = 11.84 VVLPAAEE257 pKa = 4.63 DD258 pKa = 3.87 GVYY261 pKa = 10.28 VPSSVEE267 pKa = 3.64 EE268 pKa = 4.4 SPAPEE273 pKa = 4.86 AEE275 pKa = 4.03 AVPEE279 pKa = 4.22 PEE281 pKa = 5.06 AEE283 pKa = 4.22 VAPEE287 pKa = 4.63 LEE289 pKa = 4.68 PEE291 pKa = 4.23 AAPVPEE297 pKa = 5.01 PEE299 pKa = 4.57 SEE301 pKa = 4.31 VAPEE305 pKa = 4.49 PEE307 pKa = 4.52 PEE309 pKa = 4.04 AAPEE313 pKa = 3.84 PVLEE317 pKa = 4.48 PEE319 pKa = 4.66 EE320 pKa = 4.2 PASPEE325 pKa = 3.71 VDD327 pKa = 3.34 VVPEE331 pKa = 4.24 PEE333 pKa = 4.37 SEE335 pKa = 4.3 VAPEE339 pKa = 4.26 PEE341 pKa = 4.74 PEE343 pKa = 4.35 PEE345 pKa = 4.06 VAPAPEE351 pKa = 4.59 PEE353 pKa = 4.57 PEE355 pKa = 4.32 SEE357 pKa = 4.58 AEE359 pKa = 3.79 AAPAEE364 pKa = 4.41 GEE366 pKa = 4.27 FFVDD370 pKa = 5.37 SIPSEE375 pKa = 4.62 LNPQDD380 pKa = 4.83 GEE382 pKa = 4.44 TEE384 pKa = 4.11 PDD386 pKa = 3.07 HH387 pKa = 6.99 GEE389 pKa = 3.9 QSSEE393 pKa = 3.93 EE394 pKa = 4.24 SEE396 pKa = 4.71 LEE398 pKa = 3.66 SDD400 pKa = 4.15 YY401 pKa = 11.88 EE402 pKa = 3.99 EE403 pKa = 6.19 LEE405 pKa = 4.05 ATRR408 pKa = 11.84 VVPRR412 pKa = 11.84 VHH414 pKa = 6.72 KK415 pKa = 10.24 PFIAPEE421 pKa = 4.04 LQQSQGAPAPTPLEE435 pKa = 3.97 VDD437 pKa = 3.8 AAPAPSPGEE446 pKa = 4.04 FEE448 pKa = 4.61 AAPAPSPVDD457 pKa = 4.0 FEE459 pKa = 4.46 PAPAPSPEE467 pKa = 3.9 AHH469 pKa = 6.13 EE470 pKa = 4.5 VAPAPGPEE478 pKa = 4.12 DD479 pKa = 5.22 LEE481 pKa = 4.49 VAPAPSPADD490 pKa = 3.77 YY491 pKa = 10.71 EE492 pKa = 4.32 PAPAPAPTEE501 pKa = 4.1 YY502 pKa = 11.05 APAPAPNPQYY512 pKa = 10.58 EE513 pKa = 4.35 PAPAPSGVMPPAFPDD528 pKa = 3.67 VPQYY532 pKa = 10.93 RR533 pKa = 11.84 PNNEE537 pKa = 3.57 EE538 pKa = 3.76 TSQKK542 pKa = 10.58 GSSKK546 pKa = 10.64 RR547 pKa = 11.84 KK548 pKa = 9.22 LLWIILSVIVVIGLCVGGYY567 pKa = 9.45 FLYY570 pKa = 10.46 SYY572 pKa = 10.19 LASGSDD578 pKa = 3.56 NEE580 pKa = 4.51 SLGNQATSQLQSEE593 pKa = 5.19 YY594 pKa = 10.99 VPTMYY599 pKa = 10.49 FAA601 pKa = 5.55
Molecular weight: 64.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.754
IPC2_protein 3.783
IPC_protein 3.745
Toseland 3.579
ProMoST 3.846
Dawson 3.681
Bjellqvist 3.834
Wikipedia 3.528
Rodwell 3.579
Grimsley 3.49
Solomon 3.681
Lehninger 3.63
Nozaki 3.795
DTASelect 3.897
Thurlkill 3.579
EMBOSS 3.554
Sillero 3.846
Patrickios 0.947
IPC_peptide 3.681
IPC2_peptide 3.846
IPC2.peptide.svr19 3.766
Protein with the highest isoelectric point:
>tr|A0A1Q5PPC0|A0A1Q5PPC0_9ACTO Uncharacterized protein OS=Boudabousia liubingyangii OX=1921764 GN=BSR29_00315 PE=4 SV=1
MM1 pKa = 7.41 VKK3 pKa = 9.03 RR4 pKa = 11.84 TFQPNNRR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 AKK15 pKa = 9.4 VHH17 pKa = 5.52 GFRR20 pKa = 11.84 KK21 pKa = 9.95 RR22 pKa = 11.84 MSTRR26 pKa = 11.84 AGRR29 pKa = 11.84 AILAARR35 pKa = 11.84 RR36 pKa = 11.84 RR37 pKa = 11.84 KK38 pKa = 9.67 GRR40 pKa = 11.84 ASISAA45 pKa = 3.59
Molecular weight: 5.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.286
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.042
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.676
Grimsley 13.086
Solomon 13.539
Lehninger 13.437
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.539
Sillero 13.042
Patrickios 12.398
IPC_peptide 13.539
IPC2_peptide 12.53
IPC2.peptide.svr19 9.215
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1617
0
1617
587279
32
3168
363.2
39.61
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.064 ± 0.087
0.745 ± 0.019
5.289 ± 0.05
6.494 ± 0.059
3.508 ± 0.038
7.876 ± 0.056
1.882 ± 0.023
5.068 ± 0.055
4.335 ± 0.055
10.35 ± 0.076
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.094 ± 0.026
3.201 ± 0.038
5.384 ± 0.06
4.22 ± 0.045
5.46 ± 0.058
5.835 ± 0.042
5.725 ± 0.048
7.505 ± 0.059
1.446 ± 0.026
2.519 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here