Gordonia phage Asapag
Average proteome isoelectric point is 6.44
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 99 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A410TDS0|A0A410TDS0_9CAUD Major capsid protein OS=Gordonia phage Asapag OX=2507862 GN=7 PE=4 SV=1
MM1 pKa = 7.2 MNRR4 pKa = 11.84 RR5 pKa = 11.84 LAIAAVCAAAACILSSCSSDD25 pKa = 3.9 SGDD28 pKa = 3.21 TAAASSSSMAPSTVEE43 pKa = 3.81 TNGRR47 pKa = 11.84 GAIEE51 pKa = 4.15 VALGEE56 pKa = 4.22 PVAITGSDD64 pKa = 3.7 GAPVLTITGTRR75 pKa = 11.84 LDD77 pKa = 3.8 STGCAGDD84 pKa = 3.79 HH85 pKa = 7.05 PEE87 pKa = 3.94 VAHH90 pKa = 5.96 TKK92 pKa = 10.09 FVATIQTGDD101 pKa = 3.42 VEE103 pKa = 4.67 TEE105 pKa = 3.5 QWLWPSDD112 pKa = 3.5 FYY114 pKa = 11.89 YY115 pKa = 11.37 VNDD118 pKa = 4.05 ANKK121 pKa = 9.42 VAQNSEE127 pKa = 4.02 IEE129 pKa = 4.3 QATDD133 pKa = 2.9 TAFACEE139 pKa = 4.18 GSVEE143 pKa = 4.82 FIDD146 pKa = 4.98 VPPNTSKK153 pKa = 11.11 DD154 pKa = 3.52 GSPTLSVPVMTTAIGYY170 pKa = 9.22 HH171 pKa = 7.11 LDD173 pKa = 3.51 TAGVDD178 pKa = 4.07 QRR180 pKa = 11.84 VEE182 pKa = 3.79 WKK184 pKa = 10.76 LPAEE188 pKa = 4.22 WRR190 pKa = 11.84 QALAPVTSEE199 pKa = 4.27 APATTTEE206 pKa = 4.15 QPAPEE211 pKa = 4.28 PTNPAPEE218 pKa = 4.12 TTAPDD223 pKa = 3.35 TAAPDD228 pKa = 3.44 SGGIPPGWDD237 pKa = 2.97 KK238 pKa = 11.77 NGDD241 pKa = 3.45 GMIDD245 pKa = 3.22 TDD247 pKa = 4.84 APIGDD252 pKa = 4.12 DD253 pKa = 4.88 CEE255 pKa = 4.46 TPEE258 pKa = 4.42 CLMGEE263 pKa = 4.58 GGG265 pKa = 3.8
Molecular weight: 27.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.98
IPC2_protein 3.846
IPC_protein 3.834
Toseland 3.63
ProMoST 3.986
Dawson 3.821
Bjellqvist 3.973
Wikipedia 3.732
Rodwell 3.668
Grimsley 3.541
Solomon 3.808
Lehninger 3.77
Nozaki 3.923
DTASelect 4.139
Thurlkill 3.668
EMBOSS 3.745
Sillero 3.948
Patrickios 2.918
IPC_peptide 3.808
IPC2_peptide 3.935
IPC2.peptide.svr19 3.876
Protein with the highest isoelectric point:
>tr|A0A410TDW7|A0A410TDW7_9CAUD DUF1508 domain-containing protein OS=Gordonia phage Asapag OX=2507862 GN=72 PE=4 SV=1
MM1 pKa = 7.74 SSPLLEE7 pKa = 4.48 GLSDD11 pKa = 3.92 LARR14 pKa = 11.84 RR15 pKa = 11.84 RR16 pKa = 11.84 WFRR19 pKa = 11.84 WWVMLWQIPSAIVLPMSLLLTHH41 pKa = 6.93 WNVALTAAGSIATALFLFALSADD64 pKa = 3.06 VDD66 pKa = 3.69 QRR68 pKa = 11.84 RR69 pKa = 11.84 KK70 pKa = 9.59 AEE72 pKa = 3.61 EE73 pKa = 4.37 RR74 pKa = 11.84 ITYY77 pKa = 10.01 RR78 pKa = 11.84 INFDD82 pKa = 2.22 VDD84 pKa = 3.08 AAMKK88 pKa = 9.42 RR89 pKa = 11.84 AEE91 pKa = 4.26 RR92 pKa = 11.84 RR93 pKa = 11.84 RR94 pKa = 11.84 MQDD97 pKa = 2.12 GGQAA101 pKa = 3.15
Molecular weight: 11.63 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.331
IPC2_protein 9.458
IPC_protein 10.628
Toseland 10.789
ProMoST 10.847
Dawson 10.847
Bjellqvist 10.672
Wikipedia 11.169
Rodwell 10.76
Grimsley 10.891
Solomon 11.125
Lehninger 11.067
Nozaki 10.774
DTASelect 10.672
Thurlkill 10.789
EMBOSS 11.228
Sillero 10.804
Patrickios 10.599
IPC_peptide 11.14
IPC2_peptide 9.809
IPC2.peptide.svr19 8.938
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
99
0
99
17781
41
1875
179.6
19.74
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.079 ± 0.43
1.181 ± 0.182
6.372 ± 0.294
5.855 ± 0.313
2.745 ± 0.179
8.025 ± 0.405
2.233 ± 0.179
4.561 ± 0.169
3.661 ± 0.273
7.823 ± 0.264
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.356 ± 0.17
2.851 ± 0.174
5.517 ± 0.206
3.532 ± 0.154
7.502 ± 0.359
5.922 ± 0.281
6.462 ± 0.305
7.564 ± 0.328
2.351 ± 0.137
2.407 ± 0.18
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here