Gordonia phage Asapag

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Langleyhallvirinae; Getalongvirus; Gordonia virus Asapag

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 99 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A410TDS0|A0A410TDS0_9CAUD Major capsid protein OS=Gordonia phage Asapag OX=2507862 GN=7 PE=4 SV=1
MM1 pKa = 7.2MNRR4 pKa = 11.84RR5 pKa = 11.84LAIAAVCAAAACILSSCSSDD25 pKa = 3.9SGDD28 pKa = 3.21TAAASSSSMAPSTVEE43 pKa = 3.81TNGRR47 pKa = 11.84GAIEE51 pKa = 4.15VALGEE56 pKa = 4.22PVAITGSDD64 pKa = 3.7GAPVLTITGTRR75 pKa = 11.84LDD77 pKa = 3.8STGCAGDD84 pKa = 3.79HH85 pKa = 7.05PEE87 pKa = 3.94VAHH90 pKa = 5.96TKK92 pKa = 10.09FVATIQTGDD101 pKa = 3.42VEE103 pKa = 4.67TEE105 pKa = 3.5QWLWPSDD112 pKa = 3.5FYY114 pKa = 11.89YY115 pKa = 11.37VNDD118 pKa = 4.05ANKK121 pKa = 9.42VAQNSEE127 pKa = 4.02IEE129 pKa = 4.3QATDD133 pKa = 2.9TAFACEE139 pKa = 4.18GSVEE143 pKa = 4.82FIDD146 pKa = 4.98VPPNTSKK153 pKa = 11.11DD154 pKa = 3.52GSPTLSVPVMTTAIGYY170 pKa = 9.22HH171 pKa = 7.11LDD173 pKa = 3.51TAGVDD178 pKa = 4.07QRR180 pKa = 11.84VEE182 pKa = 3.79WKK184 pKa = 10.76LPAEE188 pKa = 4.22WRR190 pKa = 11.84QALAPVTSEE199 pKa = 4.27APATTTEE206 pKa = 4.15QPAPEE211 pKa = 4.28PTNPAPEE218 pKa = 4.12TTAPDD223 pKa = 3.35TAAPDD228 pKa = 3.44SGGIPPGWDD237 pKa = 2.97KK238 pKa = 11.77NGDD241 pKa = 3.45GMIDD245 pKa = 3.22TDD247 pKa = 4.84APIGDD252 pKa = 4.12DD253 pKa = 4.88CEE255 pKa = 4.46TPEE258 pKa = 4.42CLMGEE263 pKa = 4.58GGG265 pKa = 3.8

Molecular weight: 27.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A410TDW7|A0A410TDW7_9CAUD DUF1508 domain-containing protein OS=Gordonia phage Asapag OX=2507862 GN=72 PE=4 SV=1
MM1 pKa = 7.74SSPLLEE7 pKa = 4.48GLSDD11 pKa = 3.92LARR14 pKa = 11.84RR15 pKa = 11.84RR16 pKa = 11.84WFRR19 pKa = 11.84WWVMLWQIPSAIVLPMSLLLTHH41 pKa = 6.93WNVALTAAGSIATALFLFALSADD64 pKa = 3.06VDD66 pKa = 3.69QRR68 pKa = 11.84RR69 pKa = 11.84KK70 pKa = 9.59AEE72 pKa = 3.61EE73 pKa = 4.37RR74 pKa = 11.84ITYY77 pKa = 10.01RR78 pKa = 11.84INFDD82 pKa = 2.22VDD84 pKa = 3.08AAMKK88 pKa = 9.42RR89 pKa = 11.84AEE91 pKa = 4.26RR92 pKa = 11.84RR93 pKa = 11.84RR94 pKa = 11.84MQDD97 pKa = 2.12GGQAA101 pKa = 3.15

Molecular weight: 11.63 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

99

0

99

17781

41

1875

179.6

19.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.079 ± 0.43

1.181 ± 0.182

6.372 ± 0.294

5.855 ± 0.313

2.745 ± 0.179

8.025 ± 0.405

2.233 ± 0.179

4.561 ± 0.169

3.661 ± 0.273

7.823 ± 0.264

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.356 ± 0.17

2.851 ± 0.174

5.517 ± 0.206

3.532 ± 0.154

7.502 ± 0.359

5.922 ± 0.281

6.462 ± 0.305

7.564 ± 0.328

2.351 ± 0.137

2.407 ± 0.18
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski