Amino acid dipepetide frequency for Gordonia phage Asapag

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.486AlaAla: 12.486 ± 0.956
1.012AlaCys: 1.012 ± 0.347
7.368AlaAsp: 7.368 ± 0.793
7.255AlaGlu: 7.255 ± 0.815
2.812AlaPhe: 2.812 ± 0.819
8.774AlaGly: 8.774 ± 0.808
1.575AlaHis: 1.575 ± 0.374
5.962AlaIle: 5.962 ± 0.731
3.881AlaLys: 3.881 ± 0.511
9.786AlaLeu: 9.786 ± 0.737
3.375AlaMet: 3.375 ± 0.541
2.812AlaAsn: 2.812 ± 0.397
5.287AlaPro: 5.287 ± 0.689
4.668AlaGln: 4.668 ± 0.622
8.155AlaArg: 8.155 ± 0.945
5.849AlaSer: 5.849 ± 0.616
6.187AlaThr: 6.187 ± 0.741
8.549AlaVal: 8.549 ± 1.079
1.969AlaTrp: 1.969 ± 0.29
3.037AlaTyr: 3.037 ± 0.413
0.0AlaXaa: 0.0 ± 0.0
Cys
1.181CysAla: 1.181 ± 0.315
0.112CysCys: 0.112 ± 0.071
1.294CysAsp: 1.294 ± 0.326
0.731CysGlu: 0.731 ± 0.245
0.169CysPhe: 0.169 ± 0.104
1.125CysGly: 1.125 ± 0.347
0.619CysHis: 0.619 ± 0.213
0.394CysIle: 0.394 ± 0.173
0.45CysLys: 0.45 ± 0.174
0.731CysLeu: 0.731 ± 0.19
0.112CysMet: 0.112 ± 0.075
0.394CysAsn: 0.394 ± 0.135
0.506CysPro: 0.506 ± 0.223
0.506CysGln: 0.506 ± 0.194
0.731CysArg: 0.731 ± 0.252
0.844CysSer: 0.844 ± 0.297
0.45CysThr: 0.45 ± 0.173
0.506CysVal: 0.506 ± 0.19
0.562CysTrp: 0.562 ± 0.198
0.394CysTyr: 0.394 ± 0.161
0.0CysXaa: 0.0 ± 0.0
Asp
6.974AspAla: 6.974 ± 0.688
0.675AspCys: 0.675 ± 0.232
4.274AspAsp: 4.274 ± 0.693
4.274AspGlu: 4.274 ± 0.542
2.137AspPhe: 2.137 ± 0.448
5.287AspGly: 5.287 ± 0.67
1.406AspHis: 1.406 ± 0.31
2.531AspIle: 2.531 ± 0.418
2.643AspLys: 2.643 ± 0.417
5.174AspLeu: 5.174 ± 0.597
1.012AspMet: 1.012 ± 0.25
1.406AspAsn: 1.406 ± 0.29
3.993AspPro: 3.993 ± 0.434
1.294AspGln: 1.294 ± 0.271
4.106AspArg: 4.106 ± 0.611
3.656AspSer: 3.656 ± 0.415
3.543AspThr: 3.543 ± 0.535
6.187AspVal: 6.187 ± 0.493
1.462AspTrp: 1.462 ± 0.305
1.687AspTyr: 1.687 ± 0.297
0.0AspXaa: 0.0 ± 0.0
Glu
7.424GluAla: 7.424 ± 0.874
0.9GluCys: 0.9 ± 0.236
3.093GluAsp: 3.093 ± 0.438
2.812GluGlu: 2.812 ± 0.406
1.575GluPhe: 1.575 ± 0.265
3.937GluGly: 3.937 ± 0.49
1.687GluHis: 1.687 ± 0.318
2.306GluIle: 2.306 ± 0.366
2.362GluLys: 2.362 ± 0.333
5.737GluLeu: 5.737 ± 0.874
1.237GluMet: 1.237 ± 0.282
1.237GluAsn: 1.237 ± 0.259
2.812GluPro: 2.812 ± 0.481
2.868GluGln: 2.868 ± 0.53
4.724GluArg: 4.724 ± 0.698
3.262GluSer: 3.262 ± 0.485
3.431GluThr: 3.431 ± 0.434
4.162GluVal: 4.162 ± 0.519
1.856GluTrp: 1.856 ± 0.483
1.125GluTyr: 1.125 ± 0.283
0.0GluXaa: 0.0 ± 0.0
Phe
3.206PheAla: 3.206 ± 0.447
0.225PheCys: 0.225 ± 0.099
1.519PheAsp: 1.519 ± 0.319
1.181PheGlu: 1.181 ± 0.218
0.731PhePhe: 0.731 ± 0.212
3.037PheGly: 3.037 ± 0.527
0.45PheHis: 0.45 ± 0.173
0.956PheIle: 0.956 ± 0.269
1.125PheLys: 1.125 ± 0.259
1.575PheLeu: 1.575 ± 0.321
0.9PheMet: 0.9 ± 0.291
0.956PheAsn: 0.956 ± 0.27
1.462PhePro: 1.462 ± 0.289
0.9PheGln: 0.9 ± 0.203
2.193PheArg: 2.193 ± 0.347
1.912PheSer: 1.912 ± 0.313
1.912PheThr: 1.912 ± 0.271
1.744PheVal: 1.744 ± 0.313
0.506PheTrp: 0.506 ± 0.169
0.956PheTyr: 0.956 ± 0.203
0.0PheXaa: 0.0 ± 0.0
Gly
6.862GlyAla: 6.862 ± 1.245
0.619GlyCys: 0.619 ± 0.185
4.668GlyAsp: 4.668 ± 0.658
3.825GlyGlu: 3.825 ± 0.495
2.868GlyPhe: 2.868 ± 0.628
7.312GlyGly: 7.312 ± 1.288
1.744GlyHis: 1.744 ± 0.345
3.937GlyIle: 3.937 ± 0.621
3.206GlyLys: 3.206 ± 0.46
5.962GlyLeu: 5.962 ± 0.576
2.137GlyMet: 2.137 ± 0.382
2.025GlyAsn: 2.025 ± 0.376
3.543GlyPro: 3.543 ± 0.378
2.756GlyGln: 2.756 ± 0.406
6.637GlyArg: 6.637 ± 0.574
5.343GlySer: 5.343 ± 0.811
5.681GlyThr: 5.681 ± 0.539
6.805GlyVal: 6.805 ± 0.874
2.531GlyTrp: 2.531 ± 0.406
1.8GlyTyr: 1.8 ± 0.3
0.0GlyXaa: 0.0 ± 0.0
His
1.8HisAla: 1.8 ± 0.299
0.281HisCys: 0.281 ± 0.129
0.956HisAsp: 0.956 ± 0.237
1.237HisGlu: 1.237 ± 0.326
0.675HisPhe: 0.675 ± 0.187
1.856HisGly: 1.856 ± 0.32
0.562HisHis: 0.562 ± 0.186
0.844HisIle: 0.844 ± 0.233
0.506HisLys: 0.506 ± 0.222
2.812HisLeu: 2.812 ± 0.516
1.012HisMet: 1.012 ± 0.244
0.394HisAsn: 0.394 ± 0.169
1.237HisPro: 1.237 ± 0.199
0.731HisGln: 0.731 ± 0.198
1.631HisArg: 1.631 ± 0.33
0.956HisSer: 0.956 ± 0.263
1.631HisThr: 1.631 ± 0.328
1.8HisVal: 1.8 ± 0.328
0.731HisTrp: 0.731 ± 0.282
0.675HisTyr: 0.675 ± 0.2
0.0HisXaa: 0.0 ± 0.0
Ile
5.512IleAla: 5.512 ± 0.611
0.45IleCys: 0.45 ± 0.189
3.543IleAsp: 3.543 ± 0.445
3.262IleGlu: 3.262 ± 0.517
0.844IlePhe: 0.844 ± 0.311
4.331IleGly: 4.331 ± 0.456
0.562IleHis: 0.562 ± 0.18
1.462IleIle: 1.462 ± 0.336
1.912IleLys: 1.912 ± 0.555
3.431IleLeu: 3.431 ± 0.431
0.619IleMet: 0.619 ± 0.227
1.631IleAsn: 1.631 ± 0.337
2.362IlePro: 2.362 ± 0.36
1.744IleGln: 1.744 ± 0.305
3.375IleArg: 3.375 ± 0.377
1.912IleSer: 1.912 ± 0.323
3.487IleThr: 3.487 ± 0.52
3.487IleVal: 3.487 ± 0.467
0.619IleTrp: 0.619 ± 0.197
1.069IleTyr: 1.069 ± 0.259
0.0IleXaa: 0.0 ± 0.0
Lys
4.274LysAla: 4.274 ± 0.552
0.337LysCys: 0.337 ± 0.148
2.418LysAsp: 2.418 ± 0.501
1.462LysGlu: 1.462 ± 0.303
1.406LysPhe: 1.406 ± 0.285
2.868LysGly: 2.868 ± 0.564
0.9LysHis: 0.9 ± 0.207
1.35LysIle: 1.35 ± 0.257
2.081LysLys: 2.081 ± 0.441
2.925LysLeu: 2.925 ± 0.293
0.394LysMet: 0.394 ± 0.126
1.181LysAsn: 1.181 ± 0.31
2.025LysPro: 2.025 ± 0.347
1.406LysGln: 1.406 ± 0.335
2.531LysArg: 2.531 ± 0.393
2.081LysSer: 2.081 ± 0.313
3.093LysThr: 3.093 ± 0.424
2.475LysVal: 2.475 ± 0.385
0.844LysTrp: 0.844 ± 0.205
0.562LysTyr: 0.562 ± 0.166
0.0LysXaa: 0.0 ± 0.0
Leu
9.505LeuAla: 9.505 ± 0.878
0.731LeuCys: 0.731 ± 0.241
5.062LeuAsp: 5.062 ± 0.702
4.274LeuGlu: 4.274 ± 0.701
2.081LeuPhe: 2.081 ± 0.499
5.568LeuGly: 5.568 ± 0.66
1.462LeuHis: 1.462 ± 0.28
3.937LeuIle: 3.937 ± 0.544
2.531LeuLys: 2.531 ± 0.334
6.468LeuLeu: 6.468 ± 0.789
1.687LeuMet: 1.687 ± 0.341
1.744LeuAsn: 1.744 ± 0.344
4.612LeuPro: 4.612 ± 0.528
2.418LeuGln: 2.418 ± 0.351
5.568LeuArg: 5.568 ± 0.673
5.287LeuSer: 5.287 ± 0.544
5.624LeuThr: 5.624 ± 0.566
5.962LeuVal: 5.962 ± 0.731
1.744LeuTrp: 1.744 ± 0.423
1.969LeuTyr: 1.969 ± 0.392
0.0LeuXaa: 0.0 ± 0.0
Met
2.925MetAla: 2.925 ± 0.485
0.394MetCys: 0.394 ± 0.161
1.069MetAsp: 1.069 ± 0.26
0.619MetGlu: 0.619 ± 0.188
0.45MetPhe: 0.45 ± 0.166
1.519MetGly: 1.519 ± 0.335
0.619MetHis: 0.619 ± 0.239
0.844MetIle: 0.844 ± 0.235
1.181MetLys: 1.181 ± 0.282
0.956MetLeu: 0.956 ± 0.251
0.394MetMet: 0.394 ± 0.141
0.956MetAsn: 0.956 ± 0.241
1.631MetPro: 1.631 ± 0.293
0.844MetGln: 0.844 ± 0.21
2.025MetArg: 2.025 ± 0.461
2.531MetSer: 2.531 ± 0.303
2.475MetThr: 2.475 ± 0.372
1.406MetVal: 1.406 ± 0.326
0.281MetTrp: 0.281 ± 0.155
0.45MetTyr: 0.45 ± 0.148
0.0MetXaa: 0.0 ± 0.0
Asn
3.6AsnAla: 3.6 ± 0.571
0.45AsnCys: 0.45 ± 0.146
1.912AsnAsp: 1.912 ± 0.357
1.181AsnGlu: 1.181 ± 0.206
0.45AsnPhe: 0.45 ± 0.132
2.418AsnGly: 2.418 ± 0.436
0.844AsnHis: 0.844 ± 0.26
1.406AsnIle: 1.406 ± 0.339
0.619AsnLys: 0.619 ± 0.182
2.137AsnLeu: 2.137 ± 0.338
0.394AsnMet: 0.394 ± 0.127
0.844AsnAsn: 0.844 ± 0.273
2.193AsnPro: 2.193 ± 0.323
1.012AsnGln: 1.012 ± 0.313
2.756AsnArg: 2.756 ± 0.474
1.575AsnSer: 1.575 ± 0.31
1.912AsnThr: 1.912 ± 0.338
1.912AsnVal: 1.912 ± 0.403
0.225AsnTrp: 0.225 ± 0.105
0.675AsnTyr: 0.675 ± 0.216
0.0AsnXaa: 0.0 ± 0.0
Pro
5.568ProAla: 5.568 ± 0.554
1.012ProCys: 1.012 ± 0.289
4.331ProAsp: 4.331 ± 0.614
4.443ProGlu: 4.443 ± 0.554
1.181ProPhe: 1.181 ± 0.281
4.556ProGly: 4.556 ± 0.414
1.125ProHis: 1.125 ± 0.194
2.362ProIle: 2.362 ± 0.409
2.756ProLys: 2.756 ± 0.428
3.262ProLeu: 3.262 ± 0.433
1.35ProMet: 1.35 ± 0.299
1.294ProAsn: 1.294 ± 0.349
2.925ProPro: 2.925 ± 0.551
1.462ProGln: 1.462 ± 0.308
3.15ProArg: 3.15 ± 0.418
3.543ProSer: 3.543 ± 0.537
3.993ProThr: 3.993 ± 0.448
4.274ProVal: 4.274 ± 0.513
0.956ProTrp: 0.956 ± 0.248
1.631ProTyr: 1.631 ± 0.402
0.0ProXaa: 0.0 ± 0.0
Gln
4.162GlnAla: 4.162 ± 0.513
0.731GlnCys: 0.731 ± 0.217
1.744GlnAsp: 1.744 ± 0.31
1.8GlnGlu: 1.8 ± 0.298
1.237GlnPhe: 1.237 ± 0.253
2.25GlnGly: 2.25 ± 0.394
0.731GlnHis: 0.731 ± 0.259
1.969GlnIle: 1.969 ± 0.312
0.731GlnLys: 0.731 ± 0.211
2.475GlnLeu: 2.475 ± 0.4
0.787GlnMet: 0.787 ± 0.206
1.237GlnAsn: 1.237 ± 0.224
1.969GlnPro: 1.969 ± 0.316
1.631GlnGln: 1.631 ± 0.311
3.431GlnArg: 3.431 ± 0.473
2.025GlnSer: 2.025 ± 0.328
1.969GlnThr: 1.969 ± 0.384
2.531GlnVal: 2.531 ± 0.41
1.181GlnTrp: 1.181 ± 0.286
0.731GlnTyr: 0.731 ± 0.188
0.0GlnXaa: 0.0 ± 0.0
Arg
6.918ArgAla: 6.918 ± 0.643
1.012ArgCys: 1.012 ± 0.287
4.499ArgAsp: 4.499 ± 0.547
4.556ArgGlu: 4.556 ± 0.588
2.25ArgPhe: 2.25 ± 0.347
5.062ArgGly: 5.062 ± 0.574
2.587ArgHis: 2.587 ± 0.608
3.656ArgIle: 3.656 ± 0.513
2.756ArgLys: 2.756 ± 0.518
6.412ArgLeu: 6.412 ± 0.578
2.025ArgMet: 2.025 ± 0.344
2.306ArgAsn: 2.306 ± 0.369
3.656ArgPro: 3.656 ± 0.571
2.756ArgGln: 2.756 ± 0.406
7.537ArgArg: 7.537 ± 0.991
3.881ArgSer: 3.881 ± 0.553
3.937ArgThr: 3.937 ± 0.45
4.781ArgVal: 4.781 ± 0.592
2.306ArgTrp: 2.306 ± 0.372
2.137ArgTyr: 2.137 ± 0.319
0.0ArgXaa: 0.0 ± 0.0
Ser
6.862SerAla: 6.862 ± 0.674
0.619SerCys: 0.619 ± 0.316
3.543SerAsp: 3.543 ± 0.525
4.106SerGlu: 4.106 ± 0.546
1.406SerPhe: 1.406 ± 0.3
6.355SerGly: 6.355 ± 0.91
1.294SerHis: 1.294 ± 0.286
2.418SerIle: 2.418 ± 0.394
1.856SerLys: 1.856 ± 0.301
4.218SerLeu: 4.218 ± 0.554
2.193SerMet: 2.193 ± 0.374
1.631SerAsn: 1.631 ± 0.317
2.925SerPro: 2.925 ± 0.342
1.631SerGln: 1.631 ± 0.285
3.6SerArg: 3.6 ± 0.585
3.881SerSer: 3.881 ± 0.778
3.825SerThr: 3.825 ± 0.617
4.781SerVal: 4.781 ± 0.698
1.012SerTrp: 1.012 ± 0.216
1.069SerTyr: 1.069 ± 0.228
0.0SerXaa: 0.0 ± 0.0
Thr
8.043ThrAla: 8.043 ± 0.865
0.619ThrCys: 0.619 ± 0.22
4.162ThrAsp: 4.162 ± 0.487
3.318ThrGlu: 3.318 ± 0.436
1.462ThrPhe: 1.462 ± 0.278
4.837ThrGly: 4.837 ± 0.567
1.687ThrHis: 1.687 ± 0.338
3.825ThrIle: 3.825 ± 0.534
1.406ThrLys: 1.406 ± 0.298
5.456ThrLeu: 5.456 ± 0.609
0.844ThrMet: 0.844 ± 0.209
2.25ThrAsn: 2.25 ± 0.298
4.781ThrPro: 4.781 ± 0.554
2.025ThrGln: 2.025 ± 0.38
3.825ThrArg: 3.825 ± 0.524
3.543ThrSer: 3.543 ± 0.578
3.6ThrThr: 3.6 ± 0.58
5.737ThrVal: 5.737 ± 0.597
1.575ThrTrp: 1.575 ± 0.328
1.575ThrTyr: 1.575 ± 0.297
0.0ThrXaa: 0.0 ± 0.0
Val
9.224ValAla: 9.224 ± 0.732
0.956ValCys: 0.956 ± 0.252
4.218ValAsp: 4.218 ± 0.562
5.737ValGlu: 5.737 ± 0.597
2.193ValPhe: 2.193 ± 0.309
5.399ValGly: 5.399 ± 0.806
1.462ValHis: 1.462 ± 0.295
3.6ValIle: 3.6 ± 0.501
2.7ValLys: 2.7 ± 0.523
4.949ValLeu: 4.949 ± 0.497
1.8ValMet: 1.8 ± 0.34
2.25ValAsn: 2.25 ± 0.427
4.443ValPro: 4.443 ± 0.533
2.812ValGln: 2.812 ± 0.456
5.062ValArg: 5.062 ± 0.605
4.612ValSer: 4.612 ± 0.691
4.556ValThr: 4.556 ± 0.629
6.13ValVal: 6.13 ± 0.666
2.025ValTrp: 2.025 ± 0.417
1.519ValTyr: 1.519 ± 0.376
0.0ValXaa: 0.0 ± 0.0
Trp
1.969TrpAla: 1.969 ± 0.373
0.281TrpCys: 0.281 ± 0.107
1.8TrpAsp: 1.8 ± 0.298
1.181TrpGlu: 1.181 ± 0.296
0.731TrpPhe: 0.731 ± 0.207
1.8TrpGly: 1.8 ± 0.367
0.45TrpHis: 0.45 ± 0.187
1.181TrpIle: 1.181 ± 0.196
0.956TrpLys: 0.956 ± 0.244
2.306TrpLeu: 2.306 ± 0.344
0.562TrpMet: 0.562 ± 0.174
1.462TrpAsn: 1.462 ± 0.335
1.406TrpPro: 1.406 ± 0.268
1.181TrpGln: 1.181 ± 0.2
2.025TrpArg: 2.025 ± 0.443
1.125TrpSer: 1.125 ± 0.232
1.237TrpThr: 1.237 ± 0.249
0.787TrpVal: 0.787 ± 0.264
0.619TrpTrp: 0.619 ± 0.223
0.45TrpTyr: 0.45 ± 0.144
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.306TyrAla: 2.306 ± 0.487
0.394TyrCys: 0.394 ± 0.162
2.25TyrAsp: 2.25 ± 0.406
1.294TyrGlu: 1.294 ± 0.317
0.787TyrPhe: 0.787 ± 0.214
1.969TyrGly: 1.969 ± 0.337
0.562TyrHis: 0.562 ± 0.177
0.675TyrIle: 0.675 ± 0.232
0.956TyrLys: 0.956 ± 0.266
1.462TyrLeu: 1.462 ± 0.317
0.675TyrMet: 0.675 ± 0.19
0.506TyrAsn: 0.506 ± 0.144
1.406TyrPro: 1.406 ± 0.329
0.675TyrGln: 0.675 ± 0.236
1.969TyrArg: 1.969 ± 0.357
1.406TyrSer: 1.406 ± 0.265
2.081TyrThr: 2.081 ± 0.346
1.631TyrVal: 1.631 ± 0.268
0.506TyrTrp: 0.506 ± 0.181
0.562TyrTyr: 0.562 ± 0.181
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 99 proteins (17781 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski