Amino acid dipepetide frequency for Glycomyces sp. MMG10089

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
22.831AlaAla: 22.831 ± 0.211
1.027AlaCys: 1.027 ± 0.026
8.67AlaAsp: 8.67 ± 0.095
10.085AlaGlu: 10.085 ± 0.11
4.123AlaPhe: 4.123 ± 0.056
11.947AlaGly: 11.947 ± 0.119
2.512AlaHis: 2.512 ± 0.05
5.273AlaIle: 5.273 ± 0.064
3.138AlaLys: 3.138 ± 0.06
13.941AlaLeu: 13.941 ± 0.117
2.737AlaMet: 2.737 ± 0.052
2.532AlaAsn: 2.532 ± 0.049
6.484AlaPro: 6.484 ± 0.094
3.47AlaGln: 3.47 ± 0.053
8.669AlaArg: 8.669 ± 0.095
6.631AlaSer: 6.631 ± 0.066
6.932AlaThr: 6.932 ± 0.072
12.183AlaVal: 12.183 ± 0.119
2.131AlaTrp: 2.131 ± 0.041
2.773AlaTyr: 2.773 ± 0.045
0.0AlaXaa: 0.0 ± 0.0
Cys
0.957CysAla: 0.957 ± 0.031
0.103CysCys: 0.103 ± 0.011
0.524CysAsp: 0.524 ± 0.021
0.48CysGlu: 0.48 ± 0.018
0.214CysPhe: 0.214 ± 0.014
0.887CysGly: 0.887 ± 0.03
0.157CysHis: 0.157 ± 0.011
0.21CysIle: 0.21 ± 0.011
0.121CysLys: 0.121 ± 0.009
0.649CysLeu: 0.649 ± 0.023
0.13CysMet: 0.13 ± 0.009
0.16CysAsn: 0.16 ± 0.011
0.399CysPro: 0.399 ± 0.019
0.147CysGln: 0.147 ± 0.01
0.489CysArg: 0.489 ± 0.019
0.437CysSer: 0.437 ± 0.019
0.416CysThr: 0.416 ± 0.019
0.569CysVal: 0.569 ± 0.021
0.11CysTrp: 0.11 ± 0.009
0.155CysTyr: 0.155 ± 0.011
0.0CysXaa: 0.0 ± 0.0
Asp
8.133AspAla: 8.133 ± 0.101
0.445AspCys: 0.445 ± 0.017
3.727AspAsp: 3.727 ± 0.065
4.372AspGlu: 4.372 ± 0.058
2.064AspPhe: 2.064 ± 0.044
6.037AspGly: 6.037 ± 0.08
1.415AspHis: 1.415 ± 0.036
1.995AspIle: 1.995 ± 0.045
1.126AspLys: 1.126 ± 0.03
7.214AspLeu: 7.214 ± 0.069
0.94AspMet: 0.94 ± 0.028
1.115AspAsn: 1.115 ± 0.027
4.867AspPro: 4.867 ± 0.069
1.727AspGln: 1.727 ± 0.04
5.047AspArg: 5.047 ± 0.069
2.549AspSer: 2.549 ± 0.049
3.097AspThr: 3.097 ± 0.048
4.119AspVal: 4.119 ± 0.056
1.145AspTrp: 1.145 ± 0.033
1.492AspTyr: 1.492 ± 0.038
0.0AspXaa: 0.0 ± 0.0
Glu
9.547GluAla: 9.547 ± 0.113
0.396GluCys: 0.396 ± 0.017
3.593GluAsp: 3.593 ± 0.06
3.803GluGlu: 3.803 ± 0.065
1.9GluPhe: 1.9 ± 0.044
5.105GluGly: 5.105 ± 0.064
1.716GluHis: 1.716 ± 0.034
2.597GluIle: 2.597 ± 0.048
1.273GluLys: 1.273 ± 0.037
6.524GluLeu: 6.524 ± 0.07
0.959GluMet: 0.959 ± 0.025
1.204GluAsn: 1.204 ± 0.031
4.067GluPro: 4.067 ± 0.074
2.189GluGln: 2.189 ± 0.04
5.788GluArg: 5.788 ± 0.084
3.249GluSer: 3.249 ± 0.055
3.556GluThr: 3.556 ± 0.052
4.987GluVal: 4.987 ± 0.066
0.944GluTrp: 0.944 ± 0.022
1.338GluTyr: 1.338 ± 0.033
0.0GluXaa: 0.0 ± 0.0
Phe
4.513PheAla: 4.513 ± 0.06
0.244PheCys: 0.244 ± 0.013
2.557PheAsp: 2.557 ± 0.042
1.897PheGlu: 1.897 ± 0.04
0.935PhePhe: 0.935 ± 0.028
3.433PheGly: 3.433 ± 0.055
0.618PheHis: 0.618 ± 0.021
1.104PheIle: 1.104 ± 0.032
0.652PheLys: 0.652 ± 0.022
2.717PheLeu: 2.717 ± 0.057
0.499PheMet: 0.499 ± 0.017
0.782PheAsn: 0.782 ± 0.024
1.324PhePro: 1.324 ± 0.032
0.804PheGln: 0.804 ± 0.025
1.684PheArg: 1.684 ± 0.037
1.572PheSer: 1.572 ± 0.034
2.26PheThr: 2.26 ± 0.038
2.195PheVal: 2.195 ± 0.042
0.474PheTrp: 0.474 ± 0.019
0.7PheTyr: 0.7 ± 0.025
0.0PheXaa: 0.0 ± 0.0
Gly
10.812GlyAla: 10.812 ± 0.121
0.732GlyCys: 0.732 ± 0.027
5.475GlyAsp: 5.475 ± 0.062
5.881GlyGlu: 5.881 ± 0.066
3.242GlyPhe: 3.242 ± 0.052
8.18GlyGly: 8.18 ± 0.132
1.991GlyHis: 1.991 ± 0.037
3.669GlyIle: 3.669 ± 0.05
2.182GlyLys: 2.182 ± 0.044
9.309GlyLeu: 9.309 ± 0.097
1.851GlyMet: 1.851 ± 0.038
1.975GlyAsn: 1.975 ± 0.045
4.977GlyPro: 4.977 ± 0.066
2.494GlyGln: 2.494 ± 0.05
6.698GlyArg: 6.698 ± 0.081
4.927GlySer: 4.927 ± 0.071
5.463GlyThr: 5.463 ± 0.066
7.015GlyVal: 7.015 ± 0.077
1.679GlyTrp: 1.679 ± 0.033
2.254GlyTyr: 2.254 ± 0.045
0.0GlyXaa: 0.0 ± 0.0
His
2.354HisAla: 2.354 ± 0.04
0.194HisCys: 0.194 ± 0.011
1.268HisAsp: 1.268 ± 0.03
1.228HisGlu: 1.228 ± 0.029
0.692HisPhe: 0.692 ± 0.021
2.032HisGly: 2.032 ± 0.041
0.653HisHis: 0.653 ± 0.023
0.643HisIle: 0.643 ± 0.024
0.322HisLys: 0.322 ± 0.015
2.502HisLeu: 2.502 ± 0.046
0.335HisMet: 0.335 ± 0.016
0.409HisAsn: 0.409 ± 0.021
1.558HisPro: 1.558 ± 0.033
0.609HisGln: 0.609 ± 0.019
1.986HisArg: 1.986 ± 0.045
0.948HisSer: 0.948 ± 0.026
1.042HisThr: 1.042 ± 0.03
1.516HisVal: 1.516 ± 0.031
0.391HisTrp: 0.391 ± 0.017
0.545HisTyr: 0.545 ± 0.021
0.0HisXaa: 0.0 ± 0.0
Ile
6.422IleAla: 6.422 ± 0.072
0.306IleCys: 0.306 ± 0.014
3.191IleAsp: 3.191 ± 0.05
2.873IleGlu: 2.873 ± 0.049
0.848IlePhe: 0.848 ± 0.022
4.361IleGly: 4.361 ± 0.053
0.618IleHis: 0.618 ± 0.019
1.286IleIle: 1.286 ± 0.037
0.745IleLys: 0.745 ± 0.024
3.091IleLeu: 3.091 ± 0.054
0.554IleMet: 0.554 ± 0.023
0.761IleAsn: 0.761 ± 0.024
1.919IlePro: 1.919 ± 0.04
0.821IleGln: 0.821 ± 0.027
2.36IleArg: 2.36 ± 0.041
1.643IleSer: 1.643 ± 0.037
2.251IleThr: 2.251 ± 0.043
3.42IleVal: 3.42 ± 0.053
0.505IleTrp: 0.505 ± 0.02
0.604IleTyr: 0.604 ± 0.023
0.0IleXaa: 0.0 ± 0.0
Lys
3.029LysAla: 3.029 ± 0.054
0.112LysCys: 0.112 ± 0.009
1.14LysAsp: 1.14 ± 0.029
1.171LysGlu: 1.171 ± 0.034
0.557LysPhe: 0.557 ± 0.024
1.655LysGly: 1.655 ± 0.04
0.484LysHis: 0.484 ± 0.021
0.944LysIle: 0.944 ± 0.028
0.632LysLys: 0.632 ± 0.03
1.987LysLeu: 1.987 ± 0.043
0.375LysMet: 0.375 ± 0.016
0.464LysAsn: 0.464 ± 0.016
1.34LysPro: 1.34 ± 0.031
0.637LysGln: 0.637 ± 0.022
1.878LysArg: 1.878 ± 0.039
1.178LysSer: 1.178 ± 0.032
1.313LysThr: 1.313 ± 0.034
1.728LysVal: 1.728 ± 0.039
0.317LysTrp: 0.317 ± 0.013
0.421LysTyr: 0.421 ± 0.019
0.0LysXaa: 0.0 ± 0.0
Leu
15.387LeuAla: 15.387 ± 0.139
0.719LeuCys: 0.719 ± 0.022
6.862LeuAsp: 6.862 ± 0.082
5.435LeuGlu: 5.435 ± 0.073
2.824LeuPhe: 2.824 ± 0.053
8.815LeuGly: 8.815 ± 0.094
2.104LeuHis: 2.104 ± 0.037
3.825LeuIle: 3.825 ± 0.061
2.106LeuLys: 2.106 ± 0.043
10.19LeuLeu: 10.19 ± 0.124
1.625LeuMet: 1.625 ± 0.031
1.94LeuAsn: 1.94 ± 0.038
5.709LeuPro: 5.709 ± 0.065
2.338LeuGln: 2.338 ± 0.043
7.742LeuArg: 7.742 ± 0.093
5.003LeuSer: 5.003 ± 0.063
6.164LeuThr: 6.164 ± 0.07
8.391LeuVal: 8.391 ± 0.087
1.43LeuTrp: 1.43 ± 0.035
1.861LeuTyr: 1.861 ± 0.039
0.0LeuXaa: 0.0 ± 0.0
Met
2.208MetAla: 2.208 ± 0.04
0.119MetCys: 0.119 ± 0.01
0.851MetAsp: 0.851 ± 0.026
0.819MetGlu: 0.819 ± 0.022
0.576MetPhe: 0.576 ± 0.022
1.271MetGly: 1.271 ± 0.034
0.357MetHis: 0.357 ± 0.016
0.783MetIle: 0.783 ± 0.025
0.438MetLys: 0.438 ± 0.022
1.818MetLeu: 1.818 ± 0.041
0.326MetMet: 0.326 ± 0.018
0.511MetAsn: 0.511 ± 0.018
1.083MetPro: 1.083 ± 0.025
0.467MetGln: 0.467 ± 0.019
1.521MetArg: 1.521 ± 0.03
1.414MetSer: 1.414 ± 0.035
1.479MetThr: 1.479 ± 0.031
1.338MetVal: 1.338 ± 0.032
0.257MetTrp: 0.257 ± 0.014
0.325MetTyr: 0.325 ± 0.017
0.0MetXaa: 0.0 ± 0.0
Asn
2.609AsnAla: 2.609 ± 0.043
0.165AsnCys: 0.165 ± 0.012
1.128AsnAsp: 1.128 ± 0.03
1.129AsnGlu: 1.129 ± 0.029
0.61AsnPhe: 0.61 ± 0.021
2.119AsnGly: 2.119 ± 0.042
0.413AsnHis: 0.413 ± 0.02
0.715AsnIle: 0.715 ± 0.023
0.349AsnLys: 0.349 ± 0.018
2.227AsnLeu: 2.227 ± 0.037
0.355AsnMet: 0.355 ± 0.016
0.482AsnAsn: 0.482 ± 0.018
1.647AsnPro: 1.647 ± 0.034
0.618AsnGln: 0.618 ± 0.024
1.522AsnArg: 1.522 ± 0.03
0.975AsnSer: 0.975 ± 0.028
1.153AsnThr: 1.153 ± 0.034
1.5AsnVal: 1.5 ± 0.039
0.391AsnTrp: 0.391 ± 0.017
0.509AsnTyr: 0.509 ± 0.02
0.0AsnXaa: 0.0 ± 0.0
Pro
7.237ProAla: 7.237 ± 0.097
0.268ProCys: 0.268 ± 0.014
4.278ProAsp: 4.278 ± 0.065
4.996ProGlu: 4.996 ± 0.074
1.631ProPhe: 1.631 ± 0.034
5.827ProGly: 5.827 ± 0.075
1.182ProHis: 1.182 ± 0.029
1.929ProIle: 1.929 ± 0.038
1.267ProLys: 1.267 ± 0.032
4.635ProLeu: 4.635 ± 0.058
0.985ProMet: 0.985 ± 0.025
1.205ProAsn: 1.205 ± 0.03
3.17ProPro: 3.17 ± 0.095
1.703ProGln: 1.703 ± 0.045
3.5ProArg: 3.5 ± 0.059
3.083ProSer: 3.083 ± 0.057
3.103ProThr: 3.103 ± 0.056
4.897ProVal: 4.897 ± 0.065
0.919ProTrp: 0.919 ± 0.028
1.194ProTyr: 1.194 ± 0.034
0.0ProXaa: 0.0 ± 0.0
Gln
3.499GlnAla: 3.499 ± 0.053
0.161GlnCys: 0.161 ± 0.012
1.198GlnAsp: 1.198 ± 0.029
1.289GlnGlu: 1.289 ± 0.03
0.845GlnPhe: 0.845 ± 0.026
1.983GlnGly: 1.983 ± 0.037
0.581GlnHis: 0.581 ± 0.023
1.43GlnIle: 1.43 ± 0.031
0.483GlnLys: 0.483 ± 0.021
2.759GlnLeu: 2.759 ± 0.047
0.553GlnMet: 0.553 ± 0.021
0.545GlnAsn: 0.545 ± 0.02
1.548GlnPro: 1.548 ± 0.05
1.088GlnGln: 1.088 ± 0.038
2.321GlnArg: 2.321 ± 0.046
1.484GlnSer: 1.484 ± 0.037
1.527GlnThr: 1.527 ± 0.034
2.34GlnVal: 2.34 ± 0.041
0.548GlnTrp: 0.548 ± 0.019
0.647GlnTyr: 0.647 ± 0.02
0.0GlnXaa: 0.0 ± 0.0
Arg
8.436ArgAla: 8.436 ± 0.098
0.52ArgCys: 0.52 ± 0.02
3.937ArgAsp: 3.937 ± 0.059
4.729ArgGlu: 4.729 ± 0.064
2.541ArgPhe: 2.541 ± 0.045
5.337ArgGly: 5.337 ± 0.089
1.892ArgHis: 1.892 ± 0.039
3.432ArgIle: 3.432 ± 0.058
1.708ArgLys: 1.708 ± 0.035
8.397ArgLeu: 8.397 ± 0.089
1.652ArgMet: 1.652 ± 0.034
1.405ArgAsn: 1.405 ± 0.032
4.31ArgPro: 4.31 ± 0.067
2.031ArgGln: 2.031 ± 0.042
7.203ArgArg: 7.203 ± 0.123
4.051ArgSer: 4.051 ± 0.058
4.256ArgThr: 4.256 ± 0.06
5.098ArgVal: 5.098 ± 0.066
1.331ArgTrp: 1.331 ± 0.033
1.728ArgTyr: 1.728 ± 0.04
0.0ArgXaa: 0.0 ± 0.0
Ser
6.458SerAla: 6.458 ± 0.07
0.374SerCys: 0.374 ± 0.019
2.949SerAsp: 2.949 ± 0.052
3.097SerGlu: 3.097 ± 0.047
1.532SerPhe: 1.532 ± 0.034
5.91SerGly: 5.91 ± 0.071
0.986SerHis: 0.986 ± 0.022
2.034SerIle: 2.034 ± 0.046
1.137SerLys: 1.137 ± 0.03
4.781SerLeu: 4.781 ± 0.059
1.075SerMet: 1.075 ± 0.024
1.076SerAsn: 1.076 ± 0.031
2.939SerPro: 2.939 ± 0.055
1.337SerGln: 1.337 ± 0.034
3.48SerArg: 3.48 ± 0.054
2.712SerSer: 2.712 ± 0.057
2.915SerThr: 2.915 ± 0.047
4.017SerVal: 4.017 ± 0.052
0.952SerTrp: 0.952 ± 0.024
1.162SerTyr: 1.162 ± 0.031
0.0SerXaa: 0.0 ± 0.0
Thr
8.3ThrAla: 8.3 ± 0.093
0.407ThrCys: 0.407 ± 0.015
3.679ThrAsp: 3.679 ± 0.053
3.744ThrGlu: 3.744 ± 0.054
1.656ThrPhe: 1.656 ± 0.039
6.374ThrGly: 6.374 ± 0.071
0.969ThrHis: 0.969 ± 0.027
2.109ThrIle: 2.109 ± 0.04
1.037ThrLys: 1.037 ± 0.028
5.228ThrLeu: 5.228 ± 0.06
0.955ThrMet: 0.955 ± 0.026
1.06ThrAsn: 1.06 ± 0.031
3.204ThrPro: 3.204 ± 0.055
1.247ThrGln: 1.247 ± 0.031
3.424ThrArg: 3.424 ± 0.052
2.803ThrSer: 2.803 ± 0.052
3.318ThrThr: 3.318 ± 0.059
5.867ThrVal: 5.867 ± 0.067
1.008ThrTrp: 1.008 ± 0.028
1.193ThrTyr: 1.193 ± 0.031
0.0ThrXaa: 0.0 ± 0.0
Val
10.901ValAla: 10.901 ± 0.104
0.693ValCys: 0.693 ± 0.021
5.279ValAsp: 5.279 ± 0.063
5.441ValGlu: 5.441 ± 0.069
2.823ValPhe: 2.823 ± 0.052
6.161ValGly: 6.161 ± 0.081
1.734ValHis: 1.734 ± 0.036
3.227ValIle: 3.227 ± 0.053
1.844ValLys: 1.844 ± 0.037
8.454ValLeu: 8.454 ± 0.089
1.398ValMet: 1.398 ± 0.036
1.924ValAsn: 1.924 ± 0.041
4.535ValPro: 4.535 ± 0.06
1.913ValGln: 1.913 ± 0.036
5.537ValArg: 5.537 ± 0.07
4.112ValSer: 4.112 ± 0.051
4.874ValThr: 4.874 ± 0.06
7.05ValVal: 7.05 ± 0.078
1.187ValTrp: 1.187 ± 0.032
1.748ValTyr: 1.748 ± 0.037
0.0ValXaa: 0.0 ± 0.0
Trp
1.817TrpAla: 1.817 ± 0.038
0.15TrpCys: 0.15 ± 0.009
0.944TrpAsp: 0.944 ± 0.03
0.877TrpGlu: 0.877 ± 0.024
0.586TrpPhe: 0.586 ± 0.021
1.185TrpGly: 1.185 ± 0.035
0.419TrpHis: 0.419 ± 0.017
0.754TrpIle: 0.754 ± 0.025
0.397TrpLys: 0.397 ± 0.017
1.734TrpLeu: 1.734 ± 0.034
0.329TrpMet: 0.329 ± 0.015
0.558TrpAsn: 0.558 ± 0.022
0.813TrpPro: 0.813 ± 0.025
0.56TrpGln: 0.56 ± 0.021
1.378TrpArg: 1.378 ± 0.029
1.036TrpSer: 1.036 ± 0.028
1.187TrpThr: 1.187 ± 0.028
1.037TrpVal: 1.037 ± 0.029
0.411TrpTrp: 0.411 ± 0.016
0.36TrpTyr: 0.36 ± 0.017
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.641TyrAla: 2.641 ± 0.049
0.179TyrCys: 0.179 ± 0.011
1.376TyrAsp: 1.376 ± 0.032
1.389TyrGlu: 1.389 ± 0.028
0.766TyrPhe: 0.766 ± 0.026
2.191TyrGly: 2.191 ± 0.052
0.425TyrHis: 0.425 ± 0.017
0.623TyrIle: 0.623 ± 0.021
0.404TyrLys: 0.404 ± 0.018
2.314TyrLeu: 2.314 ± 0.043
0.324TyrMet: 0.324 ± 0.016
0.509TyrAsn: 0.509 ± 0.021
1.117TyrPro: 1.117 ± 0.03
0.63TyrGln: 0.63 ± 0.022
1.767TyrArg: 1.767 ± 0.041
1.113TyrSer: 1.113 ± 0.028
1.275TyrThr: 1.275 ± 0.035
1.569TyrVal: 1.569 ± 0.032
0.398TyrTrp: 0.398 ± 0.017
0.538TyrTyr: 0.538 ± 0.019
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4346 proteins (1448852 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski